72 results about "Cell growth rate" patented technology

Expression systems are disclosed for the direct expression of peptide products into the culture media where genetically engineered host cells are grown. High yield was achieved with novel vectors, a special selection of hosts, and / or fermentation processes which include careful control of cell growth rate, and use of an inducer during growth phase. Special vectors are provided which include control regions having multiple promoters linked operably with coding regions encoding a signal peptide upstream from a coding region encoding the peptide of interest. Multiple transcription cassettes are also used to increase yield. The production of amidated peptides using the expression systems is also disclosed.

A first principles model may be used to simulate a batch process, and the first principles model may be used to configure a multiple-input / multiple-output control routine for controlling the batch process. The first principles model may generate estimates of batch parameters that cannot, or are not, measured during operation of the actual batch process. An example of such a parameter may be a rate of change of a component (e.g., a production rate, a cell growth rate, etc.) of the batch process. The first principles model and the configured multiple-input / multiple-output control routine may be used to facilitate control of the batch process.

The present invention relates to a stem cell derived from cambium of family Gingkoaceae and to method for the isolated culturing thereof. The stem cell derived from cambium of family Gingkoaceae according to the present invention is advantageous as it is isolated at a non-differentiated state without passing through a dedifferentiation process and stably maintained without causing a variation in the cellular growth rate and growth pattern even during a long-term culture, thereby enabling a mass culture. In addition, the stem cell derived from cambium of family Gingkoaceae according to the present invention showed an antioxidant effect similar to or higher than that of existing synthetic antioxidants when the radical-scavenging activity for scavenging radicals generated by the treatment of an oxidant is measured, and thus can be valuably used as an excellent anti-inflammatory composition.

The present invention relates to a plant stem cell derived from the cambium of family Solanaceae, and to a method for isolating and culturing the same. A stem cell derived from the cambium of family Solanaceae, according to the present invention, is useful as it can be isolated in an undifferentiated state without undergoing a dedifferentiation process and stably maintained without causing any variation in the cell growth rate and growth pattern even during long-term culture, thereby enabling large-scale culture. Moreover, the stem cell derived from the cambium of family Solanaceae and the culture thereof, according to the present invention, are useful for preventing and delaying aging as they have the effects of inducing biosynthesis of procollagen and more efficiently inhibiting the generation of enzymes related to aging than retinoic acid, which is known as having an excellent effect in skin wrinkle improvement.

The invention relates to a high-yield cultivation method of botryococcus and a production method of grease and / or hydrocarbon. The method comprises a heterotrophic or poly-culture (also named as mixed-culture) cultivation step of botryococcus algae and a photoautotrophic cultivation step which takes algae cells obtained by the heterotrophic or poly-culture cultivation as seeds. The method disclosed by the invention can be adopted to sufficiently bring rapid growth advantages of the algae cells of the botryococcus in the heterotrophic or poly-culture stage, so that a great deal of algae is provided for the botryococcus in the heterotrophic stage, and the botryococcus quickly grows in the heterotrophic stage and is accumulated with helpful substances (grease and hydrocarbon), and therefore, important technical means are provided for solving the problems that the botryococcus is low in cell growth rate in the heterotrophic stage and low in yield of the helpful substances (grease and hydrocarbon).

The invention provides an overflow tilting novel microalgae culture system. The culture system comprises an inclined plate subsystem and a microalgae solution circulation subsystem. The inclined plate subsystem contains an inclined plate for supporting a microalgae solution thin-layer, a support frame for supporting the inclined plate and an overflow device used for receiving a microalgae solution and supplying the microalgae solution to the inclined plate. The microalgae solution circulating subsystem contains a device used for conveying the microalgae solution to the overflow device and a collecting unit used for collecting the microalgae solution flowing off from the inclined plate device. The invention also relates to a microalgae culture method. According to the culture method, a microalgae solution is conveyed into an overflow groove through a microalgae solution conveying power source, and the microalgae solution overflows along the inclined plate into a microalgae accumulating groove under the effect of gravity, so as to realize microalgae culture. The culture system of the invention has a simple structure, is low-cost, is convenient for processing and manufacturing, and is simple to operate. What is more crucial is that the culture system has large unit-volume microalgae solution sunlight area and good sunlight illuminating angle so as to effectively enhance light use efficiency of microalgae cells and microalgae cell growth rate. Thus, low-cost, large-scale, high-density and efficient culture of microalgae is realized.

The invention relates to a method for culturing red algae with high-yield phycocyanin. The method comprises the steps of red algae heterotrophy or polyculture and photoautotrophic cultivation. The photoautotrophic cultivation is performed by taking algae cells obtained by heterotrophism or polyculture as seeds. The invention further relates to a method for quickly accumulating phycocyanin. By adoption of the method, the advantage that the algae cells grow quickly when the red algae are at a heterotrophy or polyculture stage, and plenty of algae seeds are provided for a follow-up photoautotrophic stage. The red algae grow quickly at the photoautotrophic stage, and phycocyanin is accumulated. According to the method, the problems that in the existing photoautotrophic cultivation process of the red algae, the cell growth rate is too low and the content and yield are low when microalgae are utilized to produce phycocyanin can be solved.

The invention discloses a cottonseed protein for fermentation and a preparation method and application thereof. A nutrient solution is sprayed to wet cottonseed meal, and then the cottonseed protein is obtained by drying and velvet removal, wherein every 100mL of nutrient solution contains 1.0-5.0g of enzymolysis cottonseed peptone, 1.0-40g of betaine, 1.0-10g of CuSO4.5H2O, 1.0-10g of MgSO4.7H2O, 0.1-1.0g of CoCl2.7H2O and 0.01-0.5g of Triton X100. According to the invention, thallus reproduction is promoted while maintaining the advantages of the fermentation technology of the existing cottonseed protein product, the cell growth rate is improved, the biomass of the growing period is increased, and the growing period is shortened; and meanwhile, the release of metabolite from the cell inside to outside can be promoted so as to increase the output of target products and even improve the product quality or shorten the fermentation period.

The invention discloses a construction method of an HDAC8 gene knockout BHK-21 cell line, which comprises the following steps: carrying out gene knockout on HDAC8 in a cell line BHK-21 for foot-and-mouth disease vaccine production by utilizing a CRISPR / Cas9 technology, transfecting BHK-21 cells by utilizing CRISPR plasmids for knocking out the HDAC8, and performing separation to obtain a plurality of cell clones by utilizing antibiotic screening in combination with gradient dilution and a cloning ring method. After genome DNA is extracted, PCR amplification and sequencing are carried out, and cell clones of which two HDAC8 genes are subjected to homozygous frameshift mutation are successfully identified. In the HDAC8 knockout BHK-21 cell line, the replication rate of foot-and-mouth disease virus is obviously accelerated, the final virus titer is obviously improved, and the HDAC8 knockout has no obvious influence on the cell growth rate, which shows that the HDAC8 knockout BHK-21 cell line has the prospect of being used for foot-and-mouth disease vaccine production. A foundation is laid for further knocking out HDAC8 from suspension culture type BHK-21 cells and directly applying HDAC8 to production of foot-and-mouth disease vaccines.

Method for simultaneous selection and maintenance of a selected microbial consortium for polyhydroxyalkanoates (PHA) production in only one step, said microbial consortium being fed with at least one readily biodegradable carbon substrate, comprising a first step of aerobic cultivation of said microbial consortium in a mixed biological reactor. According to the invention, the specific cell growth rate μl of said microbial consortium is fixed at a target value, in said first step of aerobic cultivation and in that said first step of aerobic cultivation is performed under nutrient limitation such as the readily biodegradable carbon substrate uptake rate qS1 unbalanced with said fixed specific cell growth rate μ1.

The invention discloses a construction method of an HDAC3 gene knockout BHK-21 cell line, which comprises the following steps: carrying out gene knockout on HDAC3 in a cell line BHK-21 for foot-and-mouth disease vaccine production by utilizing a CRISPR / Cas9 technology, transfecting BHK-21 cells by utilizing CRISPR plasmids for knocking out the HDAC3, and separating the cells to obtain a plurality of cell clones by utilizing antibiotic screening in combination with gradient dilution and a cloning ring method; extracting the genome DNA, performing PCR amplification and sequencing, and successfully identifying the cell clones of which two HDAC3 genes are subjected to homozygous frameshift mutation. In the HDAC3 knockout BHK-21 cell line, the replication rate of foot-and-mouth disease virus is obviously accelerated, the final virus titer is obviously improved, and the HDAC3 knockout has no obvious influence on the cell growth rate, so that the HDAC3 knockout BHK-21 cell line has the prospect of being used for foot-and-mouth disease vaccine production. Therefore, the foundation is laid for further knocking out HDAC3 from suspension culture type BHK-21 cells and directly applying HDAC3 to foot-and-mouth disease vaccine production.

The present invention relates to a process for producing biodegradable polymeric materials including polyhydroxyalkanoates (PHAs) by using the cell debris left from PHA recovery and purification. The process comprises: (a) cultivating PHA-producing microbial cells in a medium solution containing an organic carbon source to form PHAs that are accumulated in the cells as inclusion bodies; (b) harvesting the cells from the spent medium and solubilizing the non-PHA cell mass to obtain a PHA solid and a cell debris solution; (c) separating the PHA solid from the cell debris solution; (d) feeding the cell debris solution to the cultivation step (a). By reusing the cell debris generated from PHA recovery, the invention avoids disposal of a large amount of aqueous waste. In addition, a remarkable increase of cell growth and PHA synthesis is achieved, because the cell debris can be readily assimilated by the microbial cells as the nutrients.

The invention relates to Escherichia coli CICC 11021S having phage CICC 80001 resistance and l-aspartase activity and capable of transforming fumaric acid to produce l-aspartic acid. The Escherichia coli CICC 11021S is capable of effectively resisting the pollution of phage CICC 80001, excellent in cell growth rate and therefore capable of effectively improving the economic benefit of l-aspartic acid production enterprises.

The invention provides a preparation method and culture medium of a saussurea medusa high yielding flavone cell line. The method comprises the following steps: inducing callus tissue through a saussurea medusa seed; performing solid successive transfer culture on obtained callus tissue; directly selecting a cell line with high total flavone content in the callus tissue subjected to successive transfer culture through a visual method; performing liquid culture, performing solid culture from liquid culture, and continuously performing repeated screening and subculture, so as to obtain a high yielding cell line which is rapid in development and high in total flavone content. The cell line and the culture medium obtained through the method have the advantages that the cell growth rate can reach 45g to 50g dry weight / liter / month when the lighting suspension culture temperature is about 25 DEG C, and the content of flavonoid can reach 12 percent of the dry weight of culture.

The present invention discloses a microbial growth phenotype predication method based on a control-metabolic network integration model. The method comprises: firstly, constructing a gene control network, running multiple linear regression based on a large number of gene expression profile data so as to deduce a linear equation of expression variation of each target gene with a transcription factor, and calculating a fault discovery rate (FDR); and then taking a control relation (the FDR is less than or equal to 0.05) as a global control network, finding out transcription factors of control metabolic genes in the control network, calculating a reaction flow value F corresponding to a growth rate during a knockout period of the transcription factors according to the types of the transcription factors, performing same flow equilibrium analysis by an original metabolic network so as to obtain a maximal cell growth rate Fmax, and predicating growth phenotype changes of microorganisms during the knockout period of the transcription factors by calculating F / Fmax. The method provided by the present invention enables analysis precision to be improved, so that a growth phenotype of the microorganisms can be better predicted.

The invention discloses a construction method of an HDAC5 gene knockout BHK-21 cell line, which comprises the following steps: carrying out gene knockout on HDAC5 in a cell line BHK-21 for foot-and-mouth disease vaccine production by utilizing a CRISPR / Cas9 technology, transfecting BHK-21 cells by utilizing CRISPR plasmids for knocking out the HDAC5, and separating the cells to obtain a plurality of cell clones by utilizing antibiotic screening in combination with gradient dilution and a cloning ring method; extracting the genome DNA, performing PCR amplification and sequencing and successfully identifying the cell cloning of which one HDAC5 gene is subjected to homozygous frameshift mutation, wherein the HDAC5-KO-A2 has deletion of 13 basic groups at a Cas9 predetermined cutting position. In the HDAC5 knockout BHK-21 cell line, the replication rate of foot-and-mouth disease virus is obviously accelerated, the final virus titer is obviously improved, and the HDAC5 knockout has no obvious influence on the cell growth rate, so that the HDAC5 knockout BHK-21 cell line has the prospect of being used for foot-and-mouth disease vaccine production. Therefore, the foundation is laid for further knocking out HDAC5 from suspension culture type BHK-21 cells and directly applying HDAC5 to production of foot-and-mouth disease vaccines.

The invention belongs to the technical field of biological experiment equipment, and particularly relates to a culture device for accelerating growth rate of cells. The culture device comprises a bottom tray and a plurality of culture dish units which are sequentially arranged from bottom to top, wherein the bottom tray and the culture dish units are rotationally connected with a same connecting column; each culture dish unit can be moved along the connecting column up and down; each culture dish unit is of a bulge boss-shaped structure with a small bottom part and a large upper part, namely that each culture dish unit comprises a dish body with larger outer diameter and a dish bottom with smaller outer diameter; the dish bodies and dish bottoms of the adjacent culture dish units are sheathed together in a matching way; the different culture dish units are laminated by layers; the dish bottom of the culture dish unit at the bottommost layer is sheathed into the bottom tray in a matching way. The culture device has the advantages that by arranging each culture dish unit of the bulge boss-shaped structure, the connecting column and the bottom tray, the multiple stabilizing function is realized; the problem of easiness in scattering or turnover after the stacking of multiple common culture dishes is effectively solved.

The invention provides a method for improving the fermentation yield and saccharic acid conversion rate of L-phenylalanine. The method comprises the following steps of fermenting phenylalanine escherichia coli producing bacteria to obtain L-phenylalanine, and feeding amino acid chelated trace elements along with an organic nitrogen source in the fermentation process, wherein the amino acid chelated trace elements are composed of methionine chelated iron, glutamic acid chelated manganese, glutamic acid chelated zinc, tyrosine chelated copper and glutamic acid chelated calcium; and inorganic elements are changed into organic elements through chelation reaction of amino acid and metal cations, and the amino acid chelate with more stable chemical properties is formed. Therefore, trace elementions can be effectively prevented from forming insoluble compounds, the cell growth rate and the cell viability are improved, and then the yield and the saccharic acid conversion efficiency of phenylalanine are improved.

The present disclosure relates to methods and devices for automated control of cell growth rates where cell growth is measured in situ and the devices can be used as a stand-alone device or as a module in an automated environment, e.g., as one module in a multi-station or multi-module cell processing environment. The cell growth device comprises a temperature-controlled vial, a motor assembly to spin the vial, a spectrophotometer for measuring, e.g., OD of the cells in the vial, and a processor to accept input from a user and control the growth rate of the cells.

The invention discloses a construction method of an HDAC2 gene knockout BHK-21 cell line. The construction method comprises the following steps: carrying out gene knockout on HDAC2 in a cell line BHK-21 for foot-and-mouth disease vaccine production by utilizing a CRISPR / Cas9 technology; the inventor transfects BHK-21 cells with CRISPR plasmids for knocking out HDAC2, and then separates a plurality of cell clones by using a cloning ring method by combining antibiotic screening with gradient dilution; after genome DNA is extracted, PCR amplification and sequencing are carried out, and cell cloning of which one HDAC2 gene is subjected to homozygous frameshift mutation is successfully recognized; wherein the HDAC2-KO-B3 has the deletion of one basic group at the predetermined cutting position of the Cas9. In the BHK-21 cell line with HDAC2 knockout, the replication rate of foot-and-mouth disease virus is obviously accelerated, the final virus titer is obviously improved, and the cell growth rate is not obviously influenced after HDAC2 knockout, so that the HDAC2 knockout BHK-21 cell line has the prospect of being used for foot-and-mouth disease vaccine production. A foundation is laid for further knocking out HDAC2 from suspension culture type BHK-21 cells and directly applying HDAC2 to production of foot-and-mouth disease vaccines.

The invention belongs to the field of molecular biology and cytobiology, and provides an Ago2 gene knockout BHK-21 cell line. The preservation number of the cell line is CCTCC NO:C2021206. The Ago2 gene knockout BHK-21 cell line can be used for Seneca proliferation and production of Seneca vaccines. The Ago2 gene knockout BHK-21 cell line is successfully constructed by using a CRISPR / Cas9 technology; the replication rate of Seneca virus in the cell line is increased, the virus titer is remarkably increased, and the cell growth rate is not remarkably influenced, so that the cell line can be used for commercial production of the Seneca vaccines.

The invention provides a preparation method of a wild antheraea pernyi silk fibroin composite maltodextrin active stent material. The composite stent material takes wild antheraea pernyi silk fibroin and maltodextrin as main raw materials; the wild antheraea pernyi silk fibroin can promote fiber cell adhesion; the maltodextrin has functions of material modifier, pore-foaming agents, cell nutrients and neutrophil chemotactic agents; pore size distribution and porosity of the material are adjusted by the aid of phase separation-ultrasonic leaching hole technology in the preparation process of the material, mechanical property and degradation property of the material are favorably and flexibly adjusted, and matching of stent degradation rate and cell growth rate is realized; the preparation method is simple in process, short in production circle, high in clinical application value and especially suitable for preparation of artificial skin.

An embodiment of the invention discloses a wireless service growth estimation method and a wireless service growth estimation device. The wireless service growth estimation method comprises the following steps of: calculating self-busy time mean value indexes of a single cell in consecutive pre- and post-preset days and a post-self-busy time index of the single cell in the post-preset days according to original data of a wireless service, and further calculating to obtain a cell satisfying capacity expansion in a current network and a frequency satisfying capacity expansion in the post-presetdays; acquiring a target cell which does not meet the capacity expansion in the current network according to the frequency and the cell which meets the capacity expansion, calculating a growth rate ofeach index of the target cell, and calculating a cell growth rate of the target cell according to the growth rate of each index of the target cell; and carrying out wireless service growth estimationaccording to the cell growth rate. Through calculating the cell indexes based on the original data, the estimated data is closer to the real level of the network, and the estimation accuracy rate ishigh; differentiation of different scenes can be reflected by calculating the indexes of the single cell in consecutive pre- and post-preset days; and wireless service growth estimation is performed through utilizing the growth rate, so that the estimation efficiency can be greatly improved, and the time consumption is reduced.

The invention relates to the technical fields of fermentation engineering and aquaculture, and especially relates to a method for high-efficiency and high-density culture of a biological bait. The method comprises the following steps: 1, performing batch culture; 2, performing feeding-hunger cycle culture: feeding a medium when the cell density reaches 80-100 g / L until the pH value is 6-8, stopping the feeding for 2-8 h to adjust the cell metabolism to a hungry state, afresh recovering the feeding of the medium to recover the cell metabolism to a nutrition metabolism state, and repeating aboveprocesses; and 3, inducing the target product: going to an induction stage when the cell density reaches 100 g / L or above, and reducing the feeding rate or removing at least one substrate nutrient component from the medium to reduce the cell growth rate and induce the synthesis of the target product, wherein the substrate nutrient component can be one or more of a carbon source, a nitrogen source, a phosphorus source, a sulfur source, vitamins and heavy metal ions. The method has the advantages of high culture efficiency, one-step realization of protein accumulation, and low harvesting cost.

Expression systems are disclosed for the direct expression of peptide products into the culture media where genetically engineered host cells are grown. High yield was achieved with a special selection of hosts, and / or fermentation processes which include careful control of cell growth rate, and use of an inducer during growth phase. Special universal cloning vectors are provided for the preparation of expression vectors which include control regions having multiple promoters linked operably with coding regions encoding a signal peptide upstream from a coding region encoding the peptide of interest. Multiple transcription cassettes are also used to increase yield. The production of amidated peptides using the expression systems is also disclosed.

The present disclosure relates to methods for control of cell growth rates where cell growth is measured in situ. The methods are applicable to bacterial cells, mammalian cells, non-mammalian eukaryotic cells, plant cells, yeast cells, fungi, and archea.