Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

High-yield cultivation method of botryococcus

A kind of botrytis culture, botrytis technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, single-cell algae, etc., can solve the problem of low cell growth rate and yield, reduce the number of devices and equipment, and reduce area production The effect of high efficiency and small footprint

Inactive Publication Date: 2014-03-19
JIAXING ZEYUAN BIOLOGICAL PROD
View PDF2 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Adopting the method of the present invention can give full play to the advantage of the rapid growth of botrytis algae cells in the heterotrophic or polytrophic stage, provide a large number of algae species for the microalgae in the photoautotrophic stage and the microalgae to grow rapidly and accumulate useful substances in the photoautotrophic stage ( oils and hydrocarbons) and obtain high cell yields and metabolite yields, providing an important technical means to solve the problem of low cell growth rate and low yields of useful substances (oils and hydrocarbons) in the process of botrytis photoautotrophic culture

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High-yield cultivation method of botryococcus
  • High-yield cultivation method of botryococcus
  • High-yield cultivation method of botryococcus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] Example 1: Research on algae cell growth during heterotrophic, polytrophic and photoautotrophic culture of Botryococcus braunii cells

[0092] The botrytis of this example was cultured in heterotrophic, polytrophic and photoautotrophic cultures respectively in 500 ml shake flasks. The inoculation density of botrytis heterotrophic culture is 0.05g / l, the temperature is 25°C, and the rotational speed is 150rmp. The conditions of botrytis polyculture culture are the same as those of heterotrophic culture, except that there is light outside, and the light intensity is 80 μmolm -2 the s -1 . After 12 days of heterotrophic and mixed cultivation of grape algae, the glucose in the culture medium is exhausted, and the algae cell density is 0.70g / l and 0.78g / L, which can be used for the seeds of the next step of photoautotrophic cultivation; the seeds of grape algae are photoautotrophic The inoculation density during cultivation is 0.05g / l, the temperature is 25°C, and the lig...

Embodiment 2

[0093] Example 2: Comparison of Heterotrophic and Polycultured Culture Processes of Brown Botrytis (Botryococcus braunii) in a 5L Bioreactor under Different Culture Process Conditions

[0094] Add the heterotrophic or mixed culture medium and water to 2.5L in a 5L bioreactor and steam sterilize it, and then insert the botrytis when the temperature drops to 25° C., and start the heterotrophic or mixed culture. During mixed culture, the external light is 700 μmolm -2 the s -1 . In the case of heterotrophic or mixed culture, feed feeding is started, and the pH is kept constant at 8.0±0.3 by controlling the continuous flow of feed medium. The feed medium consisted of an organic carbon source (glucose), a nitrogen source (KNO 3 ), plant growth hormones, and inorganic salts and other nutrient salts. The added nutrient salts are the above-mentioned corresponding medium after concentration to promote the continued growth of microalgae. At the same time, timely monitor the content o...

Embodiment 3

[0096] Embodiment 3: Research on the photoautotrophic cultivation of botrytis heterotrophic seeds, polytrophic seeds and photoautotrophic seeds in indoor 2L photobioreactor

[0097] In the present embodiment, in the indoor 2L cylindrical air-lift photobioreactor, photoautotrophic cultivation of heterotrophic, polytrophic and photoautotrophic Botrytis seeds was carried out, and the conditions of heterotrophic, polytrophic and photoautotrophic seeds were measured respectively. Algae cell density, final oil and total hydrocarbon content and yield during culture. The inoculation density of heterotrophic, polytrophic and photoautotrophic seeds is 0.3g / l, the temperature of photoautotrophic culture is 25℃, and 2% CO 2 , The ventilation volume is 0.25vvm. In polyculture and photoautotrophic culture, the light intensity is 111 μmolm -2 the s -1 , continuous light. After 12 days of photoautotrophic culture, the algal cell density of heterotrophic seeds was 1.98g / l, the total hydroc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a high-yield cultivation method of botryococcus and a production method of grease and / or hydrocarbon. The method comprises a heterotrophic or poly-culture (also named as mixed-culture) cultivation step of botryococcus algae and a photoautotrophic cultivation step which takes algae cells obtained by the heterotrophic or poly-culture cultivation as seeds. The method disclosed by the invention can be adopted to sufficiently bring rapid growth advantages of the algae cells of the botryococcus in the heterotrophic or poly-culture stage, so that a great deal of algae is provided for the botryococcus in the heterotrophic stage, and the botryococcus quickly grows in the heterotrophic stage and is accumulated with helpful substances (grease and hydrocarbon), and therefore, important technical means are provided for solving the problems that the botryococcus is low in cell growth rate in the heterotrophic stage and low in yield of the helpful substances (grease and hydrocarbon).

Description

technical field [0001] The invention belongs to the field of bioenergy, and relates to a method for rapidly obtaining botrytis with high oil and / or hydrocarbon yield. Background technique [0002] Botryococcus spp. is a colony of unicellular microalgae widely distributed in freshwater in tropical and temperate regions, and occasionally in brackish water or seawater (Huang et al.1999; Volova et al. 2003). An important feature of botrytis is that it can synthesize and accumulate a large amount of various lipids, hydrocarbons (Brown and Knights1969; Knights et al.1970) and ether lipids and other components (Metzger and Casadevall1991; Metzger1999). The hydrocarbon content of Botrytis can account for 15-80% of the dry cell weight, which is much higher than that of other microorganisms (Banerjee et al. 2002). Due to their high hydrocarbon content and their ability to multiply under certain conditions (Wake and Hillen 1980; Townsend 2001), Botrytis is considered a renewable reso...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/12C12P7/64C12P5/00C12R1/89
CPCC12N1/12C12P7/64
Inventor 李元广王伟良黄建科范建华王军梁松涛王俊潘荣华陈杰沈国敏
Owner JIAXING ZEYUAN BIOLOGICAL PROD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com