Stem cell culture perfusion device

Abstract

The invention provides a stem cell culture perfusion device which comprises a perfusion bottle, a feeder layer cell culture unit, a cell perfusion tank and a waste liquid bottle, wherein the opening of the to-be-cultured cell perfusion bottle is provided with a first filter head and an input tube; the input tube is provided with a first controller capable of displaying pH value and controlling temperature; the tail end of the input tube is immersed in the feeder layer cell culture unit; a first connecting tube, which is connected through the feeder layer cell culture unit of which the substrate is provided with a semi-permeable membrane, is provided with a second controller and a first peristaltic pump; both ends of the cell perfusion tank are respectively connected with a second connecting tube and a third connecting tube; the other end of the second connecting tube is connected with the first peristaltic pump; the other end of the third connecting tube is connected with the a second peristaltic pump; the opening of the waste liquid bottle is provided with an output tube and a second filter head; and the other end of the output tube is connected with the second peristaltic pump. Under the actions of the first controller and second controller, the temperature and pH value variations can be observed, the rate of the peristaltic pumps can be controlled, and error values caused by manual observation and recording can be avoided; and the equipment output of the cell perfusion tank can enhance the stem cell culture rate.

Description

technical field [0001] The invention belongs to a perfusion device, in particular to a perfusion device for stem cell culture. Background technique [0002] In the in vitro culture of embryonic stem cells, in addition to the normal medium, it is necessary to culture a layer of special cells (such as mouse embryonic fibroblast MEF cells, etc.), and then inoculate the stem cells to be tested on it. These special cells are feeder cells, usually fibroblasts, which can secrete some unknown essential growth factors such as leukemia inhibitory factor (leukemia inhibitory factor, LIF), stem cell factor (stem cell factor, SCF) and basic growth factor. Fibroblast growth factor (basic Fibroblast growth factor, bFGF), etc., provide better nutrition for embryonic stem cells and induced pluripotent stem cells. These factors are necessary for the survival and proliferation of stem cells, not only to increase their number, but also related to the differentiation of stem cells in vitro and ...

AI Technical Summary

Problems solved by technology

Usually in the embryonic stem cell / induced pluripotent stem cell culture system, the feeder layer cells and stem cells are in a co-culture state, and the two cannot be completely separated

If the feeder layer and stem cells are cultured separately, the factors secreted by the feeder layer cells cannot be transferred to the stem cell culture system in real time, and the operation process is too many, so most of them are not used.

The traditional co-culture method affects the purity of stem cells, which has played a very restrictive role in the application of stem cells. Improving the reaction rate of the culture device is undoubtedly the common goal of those skilled in the art

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