37 results about "Botryococcus braunii" patented technology

Acceleration of botryococcenoids and growth by concomitant provision of appropriate light, minerals, and assimilable carbon. Specifically, methods, compositions and systems for the in vitro growth of hydrocarbons in photosynthetic organisms while maintaining a biologically exclusive monocultural environment, as for example, from Botryococcus species, is disclosed. Niche-nutrients can include about 200 ppm to about 3% nitrogen, and about 100 ppm to about 15% P2O5, and about 100 ppm to about 3.5% K2O. In certain embodiments, the present invention relates to the growth of the Chlorophyta such as Botryococcus sp. in a nutrient medium that includes up to 15% phosphates, at least 3 ppm soluble iron, and up to about 70 ppm soluble zinc. Also disclosed is a substantially pure culture of Botryococcus braunii var. Showa, strain Ninsei, having the ATCC Accession No. PTA-7441, its parts, and hydrocarbons produced therefrom.

The invention discloses novel methods of producing hydrocarbons through heterotrophic cultivation of Botryococcus braunii. Also provided are novel hydrocarbon compositions. A preferred species for engineering is the microalgae species Botryococcus braunii. Additional methods of cultivation include providing certain nutrient sources.

The invention provides a grease composition containing a thickener and a base oil, wherein the base oil contains (a) a hydrocarbon oil extracted from Botryococcus braunii and / or (b) a hydrocarbon oil obtainable by hydrogenating the hydrocarbon oil (a).

The invention relates to a method for promoting the quick proliferation of Botryococcus braunii, belonging to the technical field of microbe. In the method, trace elements are added in the conventional culture medium of Botryococcus; and the culture conditions are as follows: the culture temperature is 25+ / -5 DEG C; a fluorescent lamp is used as a light source, the light intensity is 80-120mu mol.m<-2>.s<-1>, and continuous illumination is adopted. The trace elements are metal ions of vanadium, tungsten, cerium and bismuth or a mixture thereof. By adopting the method that the metal ions of vanadium, tungsten, cerium, bismuth and the like are added in the culture medium of Botryococcus, the quick proliferation of microalgae can be significantly promoted, higher biomass can be obtained within unit volume and unit time and the yield of Botryococcus can be increased. A feasible technology is provided for the microalgae biofuel industrialization.

The invention relates to a high-density culture process of autotrophic oil-producing microalgae. A fermentation method combining a partition method and a two-step method is adopted, CO2 waste gas discharged from a thermal power plant is used as a carbon source, municipal sewage after living contaminant or miscellaneous algae filtering removal is used as a culture medium, the autotrophic oil-producing microalgae is cultured in a high-density way for growth, perfectly, sea salt is added into the municipal sewage for simulating sea water so that the permeation pressure is increased, the living contaminant or miscellaneous algae pollution is prevented, and the autotrophic oil-producing microalgae is selected from one kind or several kinds of materials from autotrophic chlorella, nannochloropsis salina, botryococcus braunii, chaetoceros gracile, marine green algae, isochrysis galbana, nitzschia closterium, phaeodactylum tricornutum, dunaliella and prism-shaped algae. When the high-density culture process is adopted, high removal efficiency of nitrogen, phosphorus and CO2 can be realized, in addition, the algae concentration, the grease content and the protein content are high, the double effects of environment protection and biological resources production are reached, the operation is simple, convenient and fast, the cost is low, no pollution is caused, social and economic integrated benefits are high, and the high-density culture process is suitable for large-scale popularization and application.

The invention discloses botryococcus braunii ENN41 with collection number CGMCC No.5149. The botryococcus braunii ENN41 disclosed by the invention has the advantages of high biomass, high hydrocarbon content, high growth rate and high culture density, and is suitable for industrialization application. The invention also discloses an application of the botryococcus braunii ENN41 to biofuels and particularly to biological hydrocarbon, biological diesel or biological kerosene, an application to wastewater treatment, waste gas treatment and CO2 emission reduction, and an application to the production of pigment and animal feed.

The invention belongs to the technical field of marine organisms and provides a botryococcus braunii ethanol extract as well as a preparation method and application thereof. The botryococcus braunii ethanol extract provided by the invention is prepared by the steps of repeatedly freezing and thawing fresh botryococcus braunii, centrifuging, removing supernatant, taking precipitate, adding ethanol and performing ultrasonic extraction, can relieve the premature senility phenomenon caused by oxidative stress, promote removal of in vivo reactive oxygen free radical and obviously reduce the level of the in vivo reactive oxygen free radical, has a remarkable anti-oxidation effect and can be applied to preparation of health-care food and medicines with an anti-oxidation function. The preparation method of the botryococcus braunii ethanol extract is simple and practical, low in organic solvent consumption and suitable for industrialized production.

The invention discloses a method for obtaining botryococcus braunii single cells by combining Tween80 and ultrasonic waves, which comprises the following steps of: collecting botryococcus braunii cells in a stable stage, and incubating the botryococcus braunii cells by use of Tween80; breaking the incubated botryococcus braunii cells by use of the mechanical force of ultrasonic waves; separating to obtain a single free cell; detecting the activity of the single cell and the like. The method disclosed by the invention successfully obtains the botryococcus braunii single cells, the nutrient substances and illumination are uniformly absorbed in a culture process, the preparation time is short, and the yield of the botryococcus braunii single cells is high.

The present invention relates to Rhizobium sp. KB10 strain having properties of promoting growth of Botryococcus braunii, which is an alga capable of producing biodiesel, and also enhancing production performance of biodiesel. Mores specifically, it relates to novel Rhizobium sp. KB10 strain which has properties of promoting growth of Botryococcus braunii used for biodiesel production and also enhancing content of C18 (i.e., oleate) corresponding to high quality biodiesel component as much as 900%. By using root colonizing bacteria like Rhizobium, it is possible to promote effectively the slow cell growth of Botryococcus braunii and increase as much as possible the oleate amount, which is a high quality biodiesel component. Further, by carrying out mixture culture using such bacteria, problems associated with contamination by other microorganisms during a process of producing biodiesel by culture in an outside environment can be dramatically solved.