37 results about "Botryococcus braunii" patented technology

The invention discloses novel methods of producing hydrocarbons through heterotrophic cultivation of Botryococcus braunii. Also provided are novel hydrocarbon compositions. A preferred species for engineering is the microalgae species Botryococcus braunii. Additional methods of cultivation include providing certain nutrient sources.

The invention relates to a high-density culture process of autotrophic oil-producing microalgae. A fermentation method combining a partition method and a two-step method is adopted, CO2 waste gas discharged from a thermal power plant is used as a carbon source, municipal sewage after living contaminant or miscellaneous algae filtering removal is used as a culture medium, the autotrophic oil-producing microalgae is cultured in a high-density way for growth, perfectly, sea salt is added into the municipal sewage for simulating sea water so that the permeation pressure is increased, the living contaminant or miscellaneous algae pollution is prevented, and the autotrophic oil-producing microalgae is selected from one kind or several kinds of materials from autotrophic chlorella, nannochloropsis salina, botryococcus braunii, chaetoceros gracile, marine green algae, isochrysis galbana, nitzschia closterium, phaeodactylum tricornutum, dunaliella and prism-shaped algae. When the high-density culture process is adopted, high removal efficiency of nitrogen, phosphorus and CO2 can be realized, in addition, the algae concentration, the grease content and the protein content are high, the double effects of environment protection and biological resources production are reached, the operation is simple, convenient and fast, the cost is low, no pollution is caused, social and economic integrated benefits are high, and the high-density culture process is suitable for large-scale popularization and application.

The invention discloses a culture method for efficiently inducing lipid accumulation in Botryococcus braunii, which comprises the following steps of: preparing a culture medium and daily added nutrient solution, culturing the Botryococcus braunii for 10 to 18 days of biomass accumulation, introducing CO2 into the Botryococcus braunii culture solution, regulating the concentration of the added nutrient solution, performing ultrasonic treatment on the Botryococcus braunii culture solution for 1 to 3 times by using an ultrasonic instrument at a certain frequency, spacing 10 seconds each time, and treating 5 to 60 seconds each time to inducing the lipid accumulation in the Botryococcus braunii. For the Botryococcus braunii cultured by the method, the biomass is improved by 1 to 1.5 times, thetotal fat content is improved by 30 to 55 percent, and the growth cycle is shortened by 2 to 3 days. A carbon source is supplemented in a mode of introducing CO2; and in the process of producing the lipid accumulation in the Botryococcus braunii on a large scale, a great amount of CO2 waste gas generated in the industrial production process is absorbed, and the method has significance for saving energy, reducing emission and protecting environment.

The invention discloses a method for producing biodiesel raw materials from Botryococcus braunii. The method includes the steps of (1), seed culture, (2), seed collection, (3), photoautotrophic culture, (4), microalgae harvest and (5) grease extraction. The method is used for culturing algae cells capable of controlling the oil content and the high-grease quality through introduction of indexes for representing the biodiesel quality, such as a cetane number and a ratio of unsaturated fatty acid to saturated fatty acid in terms of a culture condition and a culture technology of microalgae, and the grease content of the finally obtained algae cells is above 30%, a CN value is stabilized above 50, and the ratio of the unsaturated fatty acid to the saturated fatty acid between 1.5 and 2.5. The method is capable of effectively controlling the output and the quality of biodiesel from source raw materials and meets the application requirements of microalgae biodiesel industrialization.

Acceleration of botryococcenoids and growth by concomitant provision of appropriate light, minerals, and assimilable carbon. Specifically, methods, compositions and systems for the in vitro growth of hydrocarbons in photosynthetic organisms while maintaining a biologically exclusive monocultural environment, as for example, from Botryococcus species, is disclosed. Niche-nutrients can include about 200 ppm to about 3% nitrogen, and about 100 ppm to about 15% P205, and about 100 ppm to about 3.5% K20. In certain embodiments, the present invention relates to the growth of the Chlorophyta such as Botryococcus sp. in a nutrient medium that includes up to 15% phosphates, at least 3 ppm soluble iron, and up to about 70 ppm soluble zinc. Also disclosed is a substantially pure culture of Botryococcus braunii var. Showa, strain Ninsei, having the ATCC Accession No. PTA-7441, its parts, and hydrocarbons produced therefrom.

The invention discloses a culture method for improving the yield of botryococcus polysaccharides, comprising the following steps: culturing purified botryococcus to a logarithmic growth period, selecting botryococcus liquid in logarithmic growth, performing induced mutation under the irradiation of argon ion laser, then inoculating to a BG11 liquid medium, and culturing under the conditions that the temperature is 28-33 DEG C, the illumination intensity is 43-86mumol.m<-2>.s<-1> and the illumination period is 14 hours every day; performing ultrasonic radiation treatment after culturing 2-3 days, continuously culturing the botryococcus subjected to the ultrasonic radiation treatment for 12-13 days under the conditions that the culture temperature is 28-33 DEG C, the illumination intensity is 43-86mumol.m<-2>.s<-1> and the illumination period is 14 hours every day, and performing centrifugal separation and sterile water washing on the obtained culture solution to obtain botryococcus rich in polysaccharides. By combining the argon ion laser induced mutation with the ultrasonic treatment, the yield of the cultured botryococcus is high, many polysaccharides are accumulated, and the polysaccharide content of the botryococcus can reach 35.99% of the dry weight.

The invention relates to a method for cultivating Botryococcus braunii, which is technically characterized by including: using an inorganic carbon source, a nitrogen source and a phosphorus source additionally fed to adjust the concentration of nutrient salt in waste water; allowing the concentrations of the inorganic carbon source Na2CO3, the nitrogen source KNO3 and the phosphorus source KH2PO4 to be 5-15mg / L, 10-20mg / L and 1-2mg / L respectively; placing chlorella into culture solution at the temperature of 20 DEG C to 30 DEG C with lamination intensity 3000Lx-8000Lx and brightness darkness ratio 13h; and cultivating for 30 days with the brightness darkness ratio 11h, wherein the waste water can be domestic sewage, eutrophicating river water or lake water, and aquacultural water, and the waste water is used to cultivate high-concentration Botryococcus braunii rich in greases and hydrocarbons. By the method, the waste water is used for cultivating the botryococcus braunii with high material energy.

The invention relates to a new technology for utilizing sewage to culture oil-rich algae and further to purify water, and realizing sewage resourceful processing by recovering algae and extracting microcapsule oil. By analyzing literatures correlated to sewage purification and oil-producing algae, five algae species are preliminarily screened out for experimental analysis, and the five algae species comprise scenedesmus, chlorella, botryococcus braunii, nitzschia and navicula. Chongqing Municipality local sewage is employed for investigating the adaptability of algae species to sewage, the investigation process comprises determining growth speed and removal rate of nitrogen and phosphorus, determining biomass yield and determining algae oil content, and finally screening out a most proper algae species as a further research target. Further analysis is performed on the preponderant algae species, influences of illumination intensity and sewage concentration on algae growth and pollutant removal efficiency are researched, and the oil content of algae cells is preliminarily determined.

The invention relates to a method for extracting botryococcus braunii oil from wet botryococcus braunii fronds. The method for extracting botryococcus braunii oil from wet botryococcus braunii fronds comprises carrying out an extraction process on wet botryococcus braunii fronds by glycol dimethyl ether. The method comprises the following steps of 1, carrying out filtration, flocculation, settlement and centrifugation of a botryococcus braunii culture solution to obtain wet botryococcus braunii fronds, 2, putting the wet botryococcus braunii fronds obtained by the step 1 into an extraction container, adding an ether reagent into the extraction container, and carrying out shaking treatment, 3, adding a C5-C10 alkane solvent into the extraction container, carrying out extraction with stirring, and collecting the botryococcus braunii oil-containing extraction solvent, and 4, carrying out reduced-pressure distillation of the botryococcus braunii oil-containing extraction solvent obtained by the step 3 to obtain the botryococcus braunii oil. The method provided by the invention has the advantage that wet botryococcus braunii fronds as raw materials are directly used in hydrocarbon extraction of botryococcus braunii oil so that the problem that wet microalgae has a low extraction ratio and long extraction time is solved.

The invention discloses novel methods of producing hydrocarbons through heterotrophic cultivation of Botryococcus braunii. Also provided are novel hydrocarbon compositions. A preferred species for engineering is the microalgae species Botryococcus braunii. Additional methods of cultivation include providing certain nutrient sources.

The invention discloses a culture medium and culture method for cultivating Botry coccus braunii by utilizing monosodium glutamate plant wastewater. The technique can treat monosodium glutamate plant wastewater at low cost, utilize waste, change wastes into valuable substances and obtain the Botry coccus braunii with important economic value, thereby implementing multiple purposes. The traditional culture medium for Botry coccus braunii is the Zhu's No.13 culture solution which has the defects of low growth speed, low yield, high investment, high tendency to pollution and low benefits. In the invention, a closed circular cement track tank, which has the advantages of low cost and low tendency to pollution and is easy to operate, is utilized for culture; the monosodium glutamate plant wastewater, sodium fluoride, soil extract, sea mud extract, cattle manure extract, chicken manure extract, L-arginine, potassium dihydrogen phosphate, sorbitol, mannitol, sodium thioglycolate and the like are added to the culture medium, and the organic fertilizers and inorganic fertilizers are mixed; and thus, the nutrients are more comprehensive and balanced, the growth speed of the Botry coccus braunii is greatly enhanced, and the yield is enhanced by 320%.

The invention relates to a method for promoting the quick proliferation of Botryococcus braunii, belonging to the technical field of microbe. In the method, trace elements are added in the conventional culture medium of Botryococcus; and the culture conditions are as follows: the culture temperature is 25+ / -5 DEG C; a fluorescent lamp is used as a light source, the light intensity is 80-120mu mol.m<-2>.s<-1>, and continuous illumination is adopted. The trace elements are metal ions of vanadium, tungsten, cerium and bismuth or a mixture thereof. By adopting the method that the metal ions of vanadium, tungsten, cerium, bismuth and the like are added in the culture medium of Botryococcus, the quick proliferation of microalgae can be significantly promoted, higher biomass can be obtained within unit volume and unit time and the yield of Botryococcus can be increased. A feasible technology is provided for the microalgae biofuel industrialization.

The present invention relates to Rhizobium sp. KB10 strain having properties of promoting growth of Botryococcus braunii, which is an alga capable of producing biodiesel, and also enhancing production performance of biodiesel. Mores specifically, it relates to novel Rhizobium sp. KB10 strain which has properties of promoting growth of Botryococcus braunii used for biodiesel production and also enhancing content of C18 (i.e., oleate) corresponding to high quality biodiesel component as much as 900%. By using root colonizing bacteria like Rhizobium, it is possible to promote effectively the slow cell growth of Botryococcus braunii and increase as much as possible the oleate amount, which is a high quality biodiesel component. Further, by carrying out mixture culture using such bacteria, problems associated with contamination by other microorganisms during a process of producing biodiesel by culture in an outside environment can be dramatically solved.

The invention discloses a method for preparing microalgae biodiesel. The method includes the following steps that botryococcus braunii is inoculated to a culture medium and is irradiated by using a red and blue double-color LED light supplementing lamp for 10-14 hours per day, culture is performed at the temperature of 23-25 DEG C for 7-8 days, and then botryococcus braunii cells can be collected; the collected botryococcus braunii cells are subjected to wall-breaking treatment by ultrasonic waves, extraction is performed by using an organic solvent to obtain microalgae oil, then the microalgae oil, methanol and a zeolite molecular sieve catalyst are added to a rector according to the weight ratio of 1 to 4-6 to 0.02-0.04, esterification reaction is performed to obtain an esterification product, and the esterification product is subjected to separation and purification to obtain the biodiesel. According to the method, the botryococcus braunii short in production cycle, widely distributed, strong in photosynthesis and high in oil content is used as the raw material, and the problems existing in plant oil raw materials for biodiesel development are solved. In addition, the method also has the advantages of being simple in preparation process, high in catalytic efficiency, high in oil extraction rate, high in product yield and the like, and a good foundation is laid for large-scale biodiesel industrialization.

A method for culturing suspended photosynthetic organisms is provided, comprising culturing the photosynthetic organism in a flowing thin film photobioreactor, wherein the flowing thin film photobioreactor comprises a light source and a trickle-film insert comprising screen material. In a preferred embodiment, the organism is Rhodobacter grown anaerobically; In an additional preferred embodiment, the organism is Botryococcus braunii.

The invention discloses a method for obtaining botryococcus braunii single cells by combining Tween80 and ultrasonic waves, which comprises the following steps of: collecting botryococcus braunii cells in a stable stage, and incubating the botryococcus braunii cells by use of Tween80; breaking the incubated botryococcus braunii cells by use of the mechanical force of ultrasonic waves; separating to obtain a single free cell; detecting the activity of the single cell and the like. The method disclosed by the invention successfully obtains the botryococcus braunii single cells, the nutrient substances and illumination are uniformly absorbed in a culture process, the preparation time is short, and the yield of the botryococcus braunii single cells is high.

The invention provides a method for inducing a botryococcus braunii B12 strain to efficiently accumulate carotenoids by utilizing a plant growth regulator EBR. The method is characterized by adopting the following steps: 1) preparing an alga liquid culturing alga cells till the logarithmic phase by adopting 1 / 4 times concentration of BG11 nutritive salt under the conditions that the temperature is 23+ / -2 DEG C, the light intensity is 1900+ / -100lx and the light-dark ratio is 12h / 12h, adding 1 times concentration of BG11 nutritive salt every other 14+ / -1 days and shaking algae 3+ / -1 times every day; and 2) accumulating carotenoids: putting 200ml of alga solution in the logarithmic phase in a 300mL conical flask, then adding EBR till 0.01+ / -0.005mg / L and carrying out culture under the same conditions for 14+ / -1 days and inducing carotenoids to be quickly accumulated. The technology is mature, simple and practicable, is low in cost, is efficient and pollution-free and can achieve the effect of obviously increasing the yield of carotenoids of the botryococcus braunii B12 strain in a short time.

The invention provides a method for inducing Botryococcus braunii B12 to efficiently accumulate linolenic acid. The method is characterized by adopting the following steps : 1) preparation of algae solution : under the conditions of the temperature being 23+ / -2 DEG C, the light intensity being 1900+ / -1001 x and the ratio of light to dark being 12h / 12h, a 1 / 4 BG11 culture medium is adopted for culturing Botryococcus braunii B12 algal strain cells from 28 days to an exponential phase, an algae solution used for plant growth regulator induction is obtained, and BG11 nutritive salt with twice concentration is added once every 14 days during the culture period; 2) accumulation of linolenic acid: 200ml of the algae solution in the exponential phase is taken and placed into a 300ml conical flask, then a plant growth regulator ETH is added, the concentration of the plant growth regulator ETH in the algae solution is 0.01+ / -0.001 mg / L, induction treatment is performed at 23+ / -2 DEG C for 14+ / -1 days, manual shaking of the algae solution is not less than three times every day in the treatment stage, time intervals between adjacent shaking of the algae solution are not less than 2 hours, and the accumulation of linolenic acid in the algae solution is realized. The method is simple and feasible, the cost is low, and the accumulation of the linolenic acid content in the Botryococcus braunii B12 algal strain cells can be remarkably improved in a short term.

The invention belongs to the technical field of marine organisms and provides a botryococcus braunii ethanol extract as well as a preparation method and application thereof. The botryococcus braunii ethanol extract provided by the invention is prepared by the steps of repeatedly freezing and thawing fresh botryococcus braunii, centrifuging, removing supernatant, taking precipitate, adding ethanol and performing ultrasonic extraction, can relieve the premature senility phenomenon caused by oxidative stress, promote removal of in vivo reactive oxygen free radical and obviously reduce the level of the in vivo reactive oxygen free radical, has a remarkable anti-oxidation effect and can be applied to preparation of health-care food and medicines with an anti-oxidation function. The preparation method of the botryococcus braunii ethanol extract is simple and practical, low in organic solvent consumption and suitable for industrialized production.

The invention relates to a method for promoting the quick proliferation of Botryococcus braunii, belonging to the technical field of microbe. In the method, trace elements are added in the conventional culture medium of Botryococcus; and the culture conditions are as follows: the culture temperature is 25+ / -5 DEG C; a fluorescent lamp is used as a light source, the light intensity is 80-120mu mol.m<-2>.s<-1>, and continuous illumination is adopted. The trace elements are metal ions of vanadium, tungsten, cerium and bismuth or a mixture thereof. By adopting the method that the metal ions of vanadium, tungsten, cerium, bismuth and the like are added in the culture medium of Botryococcus, the quick proliferation of microalgae can be significantly promoted, higher biomass can be obtained within unit volume and unit time and the yield of Botryococcus can be increased. A feasible technology is provided for the microalgae biofuel industrialization.

The invention discloses a culture method for efficiently inducing lipid accumulation in Botryococcus braunii, which comprises the following steps of: preparing a culture medium and daily added nutrient solution, culturing the Botryococcus braunii for 10 to 18 days of biomass accumulation, introducing CO2 into the Botryococcus braunii culture solution, regulating the concentration of the added nutrient solution, performing ultrasonic treatment on the Botryococcus braunii culture solution for 1 to 3 times by using an ultrasonic instrument at a certain frequency, spacing 10 seconds each time, and treating 5 to 60 seconds each time to inducing the lipid accumulation in the Botryococcus braunii. For the Botryococcus braunii cultured by the method, the biomass is improved by 1 to 1.5 times, thetotal fat content is improved by 30 to 55 percent, and the growth cycle is shortened by 2 to 3 days. A carbon source is supplemented in a mode of introducing CO2; and in the process of producing the lipid accumulation in the Botryococcus braunii on a large scale, a great amount of CO2 waste gas generated in the industrial production process is absorbed, and the method has significance for saving energy, reducing emission and protecting environment.

The invention relates to a high-density culture process of autotrophic oil-producing microalgae. A fermentation method combining a partition method and a two-step method is adopted, CO2 waste gas discharged from a thermal power plant is used as a carbon source, municipal sewage after living contaminant or miscellaneous algae filtering removal is used as a culture medium, the autotrophic oil-producing microalgae is cultured in a high-density way for growth, perfectly, sea salt is added into the municipal sewage for simulating sea water so that the permeation pressure is increased, the living contaminant or miscellaneous algae pollution is prevented, and the autotrophic oil-producing microalgae is selected from one kind or several kinds of materials from autotrophic chlorella, nannochloropsis salina, botryococcus braunii, chaetoceros gracile, marine green algae, isochrysis galbana, nitzschia closterium, phaeodactylum tricornutum, dunaliella and prism-shaped algae. When the high-density culture process is adopted, high removal efficiency of nitrogen, phosphorus and CO2 can be realized, in addition, the algae concentration, the grease content and the protein content are high, the double effects of environment protection and biological resources production are reached, the operation is simple, convenient and fast, the cost is low, no pollution is caused, social and economic integrated benefits are high, and the high-density culture process is suitable for large-scale popularization and application.

The invention discloses botryococcus braunii ENN41 with collection number CGMCC No.5149. The botryococcus braunii ENN41 disclosed by the invention has the advantages of high biomass, high hydrocarbon content, high growth rate and high culture density, and is suitable for industrialization application. The invention also discloses an application of the botryococcus braunii ENN41 to biofuels and particularly to biological hydrocarbon, biological diesel or biological kerosene, an application to wastewater treatment, waste gas treatment and CO2 emission reduction, and an application to the production of pigment and animal feed.

The invention belongs to the technical field of marine organisms and provides a botryococcus braunii ethanol extract as well as a preparation method and application thereof. The botryococcus braunii ethanol extract provided by the invention is prepared by the steps of repeatedly freezing and thawing fresh botryococcus braunii, centrifuging, removing supernatant, taking precipitate, adding ethanol and performing ultrasonic extraction, can relieve the premature senility phenomenon caused by oxidative stress, promote removal of in vivo reactive oxygen free radical and obviously reduce the level of the in vivo reactive oxygen free radical, has a remarkable anti-oxidation effect and can be applied to preparation of health-care food and medicines with an anti-oxidation function. The preparation method of the botryococcus braunii ethanol extract is simple and practical, low in organic solvent consumption and suitable for industrialized production.

The invention discloses a method for obtaining botryococcus braunii single cells by combining Tween80 and ultrasonic waves, which comprises the following steps of: collecting botryococcus braunii cells in a stable stage, and incubating the botryococcus braunii cells by use of Tween80; breaking the incubated botryococcus braunii cells by use of the mechanical force of ultrasonic waves; separating to obtain a single free cell; detecting the activity of the single cell and the like. The method disclosed by the invention successfully obtains the botryococcus braunii single cells, the nutrient substances and illumination are uniformly absorbed in a culture process, the preparation time is short, and the yield of the botryococcus braunii single cells is high.

The invention discloses botryococcus braunii ENN41 with collection number CGMCC No.5149. The botryococcus braunii ENN41 disclosed by the invention has the advantages of high biomass, high hydrocarbon content, high growth rate and high culture density, and is suitable for industrialization application. The invention also discloses an application of the botryococcus braunii ENN41 to biofuels and particularly to biological hydrocarbon, biological diesel or biological kerosene, an application to wastewater treatment, waste gas treatment and CO2 emission reduction, and an application to the production of pigment and animal feed.

The invention relates to the fields of biotechnology and energy, in particular to a method for extracting bio-oil by recycling botryococcus braunii cells, comprising the following steps of: (1) culturing and collecting wet fronds of botryococcus braunii; (2) putting the wet fronds into a stirring reactor, adding an extraction solvent composed of an organic solvent and an extraction assisting agent for extracting bio-oil, separating the frond cells and collecting the extraction solvent containing the bio-oil; and (3) recovering the extraction solvent from the extraction solvent containing the bio-oil collected in the step (2), and collecting the obtained bio-oil. According to the method provided by the invention, the wet fronds are directly used for extracting the bio-oil, thereby avoidingan algae powder drying step in the traditional microalgal oil extraction process, and saving the cost and energy consumption of microalgal oil extraction; and because the botryococcus braunii cells are repeatedly used, the oil accumulation speed is high, thereby shortening the time for culturing the botryococcus braunii, reducing the culturing cost, and improving the oil production intensity of the botryococcus braunii.

The present invention relates to Rhizobium sp. KB10 strain having properties of promoting growth of Botryococcus braunii, which is an alga capable of producing biodiesel, and also enhancing production performance of biodiesel. Mores specifically, it relates to novel Rhizobium sp. KB10 strain which has properties of promoting growth of Botryococcus braunii used for biodiesel production and also enhancing content of C18 (i.e., oleate) corresponding to high quality biodiesel component as much as 900%. By using root colonizing bacteria like Rhizobium, it is possible to promote effectively the slow cell growth of Botryococcus braunii and increase as much as possible the oleate amount, which is a high quality biodiesel component. Further, by carrying out mixture culture using such bacteria, problems associated with contamination by other microorganisms during a process of producing biodiesel by culture in an outside environment can be dramatically solved.