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Botryococcus braunii ethanol extract as well as preparation method and application thereof

A technology for extraction of Botrytis brauneni and ethanol, applied in the field of marine biology, can solve the problems of few applied research, achieve the effect of simple process, improve survival rate, and promote ROS scavenging

Active Publication Date: 2017-07-21
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main application of Botrytis branici is related to bio-oil production, etc. There are not many studies on its application in other aspects, and the research on its anti-oxidation has not been found after searching.

Method used

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  • Botryococcus braunii ethanol extract as well as preparation method and application thereof
  • Botryococcus braunii ethanol extract as well as preparation method and application thereof
  • Botryococcus braunii ethanol extract as well as preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0034] The preparation of embodiment 1 botrytis brannica ethanol extract

[0035] S1. Take fresh Botrytis bransiella, freeze at -40°C for 12 hours, thaw at room temperature for 12 hours, repeat freezing and thawing 4 times, centrifuge at 10000r / min for 10min, and remove the supernatant Separate from the precipitate, and take the precipitate for subsequent use;

[0036] S2. To the precipitation described in step S1, according to the solid-to-liquid ratio of precipitation and ethanol 1g / mL, add ethanol with a volume fraction of 80% for ultrasonic extraction for 3 times, the frequency of ultrasonic extraction is 40kHz, and the temperature is 4°C. The time is 25min, centrifuged at 10000r / min for 15min, the supernatant and the precipitate are separated, the supernatant is taken for vacuum drying, the product after vacuum drying is dissolved in first-grade water, and its relative ratio to Brown grape The weight concentration of algae (wet algae) is 1 gA / mL (A represents the wet alg...

Embodiment 2

[0037] The preparation of embodiment 2 botrytis brannica ethanol extract

[0038]S1. Take fresh Botrytis bransiella, freeze at -20°C for 12 hours, thaw at room temperature for 12 hours, repeat freezing and thawing 5 times, centrifuge at a speed of 5000r / min for 15min, and remove the supernatant Separate from the precipitate, and take the precipitate for subsequent use;

[0039] S2. Add ethanol with a volume fraction of 70% to the precipitation described in step S1 according to the solid-to-liquid ratio of the precipitation to ethanol 1g / mL for ultrasonic extraction 3 times, the frequency of ultrasonic extraction is 35kHz, and the temperature is 4°C. The time is 30 minutes, centrifuged at 8000r / min for 15 minutes, the supernatant and the precipitate are separated, the supernatant is taken for vacuum drying, and the product after vacuum drying is dissolved in first-grade water, and its relative to Brown grape The weight concentration of algae (wet algae) is 1 gA / mL (A represent...

Embodiment 3

[0040] The preparation of embodiment 3 botrytis brannica ethanol extract

[0041] S1. Take fresh Botrytis bransiella, freeze at -30°C for 12 hours, thaw at room temperature for 12 hours, repeat freezing and thawing 4 times, centrifuge at 8000r / min for 15min, and remove the supernatant Separate from the precipitate, and take the precipitate for subsequent use;

[0042] S2. To the precipitation described in step S1, according to the solid-to-liquid ratio of precipitation and ethanol 1g / mL, add ethanol with a volume fraction of 90% for ultrasonic extraction twice, the frequency of ultrasonic extraction is 40kHz, and the temperature is 4°C. The time is 20 minutes, centrifuged at 8000r / min for 15 minutes, the supernatant and the precipitate are separated, the supernatant is taken for vacuum drying, and the product after vacuum drying is dissolved in first-grade water to adjust its relative to Brown grape The weight concentration of algae (wet algae) is 1 gA / mL (A represents the we...

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Abstract

The invention belongs to the technical field of marine organisms and provides a botryococcus braunii ethanol extract as well as a preparation method and application thereof. The botryococcus braunii ethanol extract provided by the invention is prepared by the steps of repeatedly freezing and thawing fresh botryococcus braunii, centrifuging, removing supernatant, taking precipitate, adding ethanol and performing ultrasonic extraction, can relieve the premature senility phenomenon caused by oxidative stress, promote removal of in vivo reactive oxygen free radical and obviously reduce the level of the in vivo reactive oxygen free radical, has a remarkable anti-oxidation effect and can be applied to preparation of health-care food and medicines with an anti-oxidation function. The preparation method of the botryococcus braunii ethanol extract is simple and practical, low in organic solvent consumption and suitable for industrialized production.

Description

technical field [0001] The invention belongs to the technical field of marine organisms, and in particular relates to an ethanol extract of staphylococcus branici and its preparation method and application. Background technique [0002] Microalgae refer to those tiny algae whose shape can only be distinguished under a microscope. There are many kinds of microalgae, short growth cycle, strong adaptability, capable of photosynthetic autotrophy, and synthesize many biologically active substances with unique structures and physiological functions, such as polysaccharides, pigments, proteins, unsaturated fatty acids, sterols, and various vitamins and minerals etc. It is favored in the development and application of food health care and pharmaceutical products, and has great potential. [0003] Brown Botrytis (Botryococcus braunii), also known as cluster granules, belongs to Xanthophyta, Xanthophyceae, Mischococcales, Botryococcusaceae, Botryococcus ), is a unicellular photosynt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/02A61P39/06A23L33/105A23L5/30
CPCA23V2002/00A61K36/02A61K2236/333A61K2236/39A61K2236/51A61K2236/53A23V2200/30A23V2200/302A23V2250/21
Inventor 胡丽莉黄泽波崔慧君宋立荣余程明
Owner GUANGDONG PHARMA UNIV
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