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Method for promoting quick proliferation of Botryococcus braunii

A fast technology of botrytis, applied in the field of microorganisms, can solve the problems of long doubling time, shortening the age of botrytis, slow growth of botrytis, etc., and achieve the effects of large biomass, increase yield, and promote rapid proliferation

Active Publication Date: 2012-12-26
溧阳常大技术转移中心有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these mediums are not ideal for shortening the generation time of botrytis, increasing the growth rate, and realizing high-density cultivation, which makes the growth of botrytis slow and the doubling time is long, which is not conducive to large-scale cultivation.

Method used

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  • Method for promoting quick proliferation of Botryococcus braunii
  • Method for promoting quick proliferation of Botryococcus braunii
  • Method for promoting quick proliferation of Botryococcus braunii

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0032] Example 1 Effect of V Elements on the Growth of Botrytis 357

[0033] With the ability to provide light and CO for the growth of microalgae 2 The airlift photobioreactor was used as a container for grape algae cultivation. The effective volume of the reactor is 8.5L. The culture temperature is 25±2 ℃; fluorescent lamps are used as the light source, and the light intensity is about 120 μmol m -2 s -1 , continuous light; air flow rate of 0.85 L / min, CO 2 The flow rate is 0.15 L / min. The concentration of cells in each sample (OD 680 ), to characterize the cell growth of Botrytis in culture medium.

[0034] Store Botrytis 357 in a 250 mL glass Erlenmeyer flask as a culture algae, and culture it on a shaker until the cell OD 680 about 1.0. Microalgae were inoculated into the above-mentioned airlift photoreactor containing BGII-containing medium.

[0035] Dissolve ammonium vanadate into the prepared BGⅡ medium, so that the final concentration of elemental va...

example 2

[0040] Example 2 Effect of W element on the growth of Botrytis 357

[0041] Dissolve sodium tungstate into the prepared BGⅡ medium, so that the final concentration of element tungsten in the medium is 0 mg / mL, 10 mg / mL, respectively. -5 mg / mL, 10 -4 mg / mL, 10 -3 mg / mL, 10 -2 mg / mL, five experiments were carried out.

[0042] The rest are the same as Example 1.

[0043]The growth speed and concentration, dry cell weight of botrytis in the culture solution containing different concentrations of tungsten, and the comparison with the culture medium BGⅡ without adding the trace element of the present invention are as follows: image 3 , 4 shown.

[0044] Depend on image 3 , 4 It can be seen that when the concentration of sodium tungstate in the medium is lower than 10 -3 mg / mL, the concentration of botrytis algae cells increased. When the concentration of W element in the medium is 10 -4 mg / mL, the dry weight of algal cells is 2.2 g / L.

example 3

[0045] Example 3 Effect of Ce element on the growth of Botrytis 357

[0046] Dissolve cerium nitrate into the prepared BGⅡ medium so that the final concentrations of elemental cerium in the medium are 0 mg / mL, 10 mg / mL, respectively. -5 mg / mL, 10 -4 mg / mL, 10 -3 mg / mL, 10 -2 mg / mL, five experiments were carried out.

[0047] The rest are the same as Example 1.

[0048] The speed and concentration, dry cell weight of botrytis growing in the culture solution containing different concentrations of cerium, and the comparison with the medium BGⅡ without adding the trace elements of the present invention are as follows: Figure 5 , 6 shown.

[0049] Depend on Figure 5 , 6 It can be seen that when cerium nitrate is added to the medium, the growth of botrytis can be promoted. The optimum concentration for botrytis growth is 10 -5 mg / mL. At this time, the dry weight of algae cells can reach 2.4 g / L.

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Abstract

The invention relates to a method for promoting the quick proliferation of Botryococcus braunii, belonging to the technical field of microbe. In the method, trace elements are added in the conventional culture medium of Botryococcus; and the culture conditions are as follows: the culture temperature is 25+ / -5 DEG C; a fluorescent lamp is used as a light source, the light intensity is 80-120mu mol.m<-2>.s<-1>, and continuous illumination is adopted. The trace elements are metal ions of vanadium, tungsten, cerium and bismuth or a mixture thereof. By adopting the method that the metal ions of vanadium, tungsten, cerium, bismuth and the like are added in the culture medium of Botryococcus, the quick proliferation of microalgae can be significantly promoted, higher biomass can be obtained within unit volume and unit time and the yield of Botryococcus can be increased. A feasible technology is provided for the microalgae biofuel industrialization.

Description

technical field [0001] The invention relates to a method for promoting the rapid proliferation of microalgae, in particular to adding a catalyst capable of promoting the rapid proliferation of hydrocarbon-producing botrytis into a conventional culture medium, and belongs to the technical field of microorganisms. Background technique [0002] Brown Botrytis ( Botryococcus braunii ), also known as Conglomerate, its hydrocarbon content usually accounts for 15%-76% of the dry weight of algae biomass, which is much higher than that of other microorganisms. The composition and structure of the hydrocarbons produced by botrytis are very similar to petroleum, and it is expected to become a renewable and non-polluting alternative energy source for petroleum. [0003] Botrytis mainly obtains the main nutrient elements (N, P) and some basic trace metal elements from the aqueous solution and air in which it grows. At present, there are many kinds of medium for grape algae. Chu10 medi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/38
Inventor 单玉华唐敏徐文杰潘学林
Owner 溧阳常大技术转移中心有限公司
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