145 results about "Doubling time" patented technology

The invention discloses a method for preparing a dense enhancement type ceramic membrane based on micro-arc oxidizing and laser remelting, and relates to a method for preparing a dense enhancement type ceramic membrane on the surfaces of light metals such as magnesium, aluminium and titanium and other alloys. The method is used for solving the problems that a micro-arc oxidizing surface treatment technology for preparing the dense enhancement type ceramic membrane on the surfaces of light metals such as magnesium, aluminium and titanium and other alloys cannot have low energy consumption and high membrane performance at the same time. The method comprises steps of: 1, pretreatment of basic materials; 2, working liquid preparation treatment; 3, micro-arc oxidation; and 4, laser remelting treatment of the micro-arc oxidation (MAO) membrane, and thus obtaining a sample piece of the dense enhancement type ceramic membrane. The prepared membrane has good binding force with a base body, dense tissues, low porosity, high hardness and good corrosion resistance and wear resistance; and the porosity of the membrane can be reduced to below 3%, the harness number can be improved by double times of the original MAO membrane which is not subjected to laser remelting treatment. The method can be used for preparing the dense enhancement type ceramic membrane on the surfaces of light metals such as magnesium, aluminium and titanium and other alloys.

The invention relates to the field of biotechnology and mainly relates to a use of an early-generation human umbilical cord mesenchymal stem cell exosome in promotion of human bone mesenchymal stem cell growth. The use method is a method for promoting bone mesenchymal stem cell growth. The invention also relates to an extraction method of a human umbilical cord mesenchymal stem cell exosome. The early-generation human umbilical cord mesenchymal stem cell exosome and human bone mesenchymal stem cells are co-cultured so that human bone mesenchymal stem cell growth is obviously promoted, cell doubling time is shortened and a ratio of an S period in a cell period is increased.

The invention discloses a low-voltage traversing control method for a double-fed wind power generation system. The method comprises the following steps of: 1) detecting zero frequency transient flux component of a motor; 2) when the motor has zero frequency transient flux component outburst, controlling a rotor current transformer and a network current transformer, and attenuating the zero frequency transient flux components of the current transformers; and 3) controlling torque current and power grid reactive compensation current to inhibit the growth of harmful voltage and prevent the formation of harmful current. The method can really realize the elimination of the transient flux caused by sudden decline and sudden rise of a stator voltage and inhibit negative sequence current so as to avoid offline of the fan power generation system at the same time of protecting the rotor current transformer and the network current transformer; meanwhile, by the method, double times ultrahigh dangerous voltage of rotor induction caused by zero frequency transient flux superposition when the voltage is recovered can be prevented, and power grid-friendly intelligent low-voltage traversing is realized.

There is provided a cell culture evaluation system, a cell culture evaluation method, and a cell culture evaluation program which are capable of estimating and evaluating the lag time or the minimum doubling time and objectively and adequately determining whether or not a cell population is stimulated for proliferation by using an average projected area of a cultured cell population or the rate of increasing the ratio of the non-single-cells as an evaluation parameter when culturing the cells. Images of the cell population to be cultured statically are acquired in a culture vessel, the average projected areas of the cells are calculated from the images for the respective culture times, and the lag times at lag phase are calculated from the calculated average projected areas of the cells. The single-cells and the non-single-cells are discriminated from the images, and the increasing rate of the non-single-cells is calculated from the ratio of the non-single-cells in the cell population to determine the minimum doubling time from the increasing rate of the non-single-cells. Whether or not the cell population is stimulated for proliferation is determined from the ratio of the non-single-cells.

The invention discloses a serum-free animal origin-free culture medium for suspension culture of BHK-21 cells and a preparation method of the serum-free animal origin-free culture medium. According to the serum-free animal origin-free culture medium for suspension culture of the BHK-21 cells and the preparation method of the serum-free animal origin-free culture medium, through the addition and component optimization of amino acids, inorganic salt, vitamins, lipids, hydrolysates and the rest of additives in the culture medium, the multiplication speed of the BHK-21 cells is remarkably improved, the doubling time is shortened, and the high activity is maintained while the cells reach higher growth density. The culture medium is suitable for high-density suspension culture of the BHK-21 cells, low in cost, free of serum, free of animal origins and low in protein, the biological function of the serum is completely replaced through the addition of the ingredients including the lipids, the vitamins, the microelements, recombinant protein and the hydrolysates,, and some negative effects caused by the serum to later purification and separation of an antigen are avoided. Through optimization on the components of the culture medium, the multiplication speed of the BHK-21 cells in suspension culture is higher, the cell density is higher, the cell activity is higher, and the BHK-21 cells are more suitable for large-scale industrial production.

The invention discloses a method for synchronizing redundant time of an intelligent substation. A system for synchronizing the redundant time of the intelligent substation is of the redundant configuration with double master clocks and two sets of extension clocks. The double master clocks receive and synchronize double time source signals, the extension clocks receive and synchronize double master clock time source signals, a time service device receives and synchronizes the time source signals coming from the extension clocks, the time service device having high requirements for time synchronization and accuracy transmits the time source signals through IRIG-B and IEC61588, the effectiveness of the time source signals is judged before synchronization of the double master clocks and double time sources, synchronization of the extension clocks and the double master clocks and synchronization of the time service device and the extension clocks, and the time source signals are synchronized in a mode of carrying out regulation step by step with the fixed step length. The method meets the requirement for reliability, stability and high accuracy of time synchronization of the intelligent substation.

The invention belongs to the technical field of wine brewing and particularly relates to mild thick-flavor white spirit and a production method thereof. A brewing technique of the mild thick-flavor white spirit comprises the following steps: feeding raw materials for the first time; roughly treating the raw materials; feeding the raw materials for the second time; treating the raw materials; fermenting grains after the first to six wine taking in turn, and taking wine; double times of feeding, one time of raw material treatment, nine times of fermentation, ten times of stewing and seven times of wine taking are performed during one production period. The brewing technique is characterized in that in comparison with traditional thick-flavor white spirit, the mild thick-flavor white spirit disclosed by the invention is characterized in that the crushing rate of fed broomcorn, the amount of used yeast for preparing hard liquor, the amount of used grain wetting water and the stacking fermentation temperature are regulated; no pit bottom liquor is generated during production; the yield and the labor productivity are increased; the grain consumption and the energy consumption are reduced; the sour and bitter tastes of first and second produced wine, and the burned food taste of six and seven produced wine are reduced; the wine is mild, mellow, elegant and smooth in style, remarkable in thick flavor, and lasting in tail taste, and the fragrance can be retained in an empty cup for a long time.

The invention belongs to the field of microalgae culture and discloses a formula of a Dicrateria zhanjiangensis improved culture medium and a simple culture technique of Dicrateria zhanjiangensis. Dicrateria zhanjiangensis is traditionally cultured by using an f / 2 culture medium in an open cement pit manner, so the doubling time of algae cells is long, the grow speed is low, the investment is high, the yield is low, pollution is easily caused, and the operation is troublesome. When Dicrateria zhanjiangensis cells are cultured by using the culture medium provided by the invention and mineral water barrels, the content of unsaturated fatty acid is high, the cost is low, pollution is avoided, the operation is easy, and the workload is greatly reduced; and nutrients such as sodium hydrogen carbonate, ferric citrate, potassium sorbate, glucose, sea mud extract, soil extract, ethylenediamine tetraacetic acid complexing agent and cow dung and goat manure extract are added in the culture medium, and organic fertilizers and inorganic fertilizers are used together, so that more nutrients are available, the growth speed of the Dicrateria zhanjiangensis is greatly improved, the culture period is shortened, and the yield is improved by 92%.

The invention relates to a process for producing ginkgo extract and belongs to the technical field of plan extraction. The process comprises the following steps of: adding ginkgo leaves serving as raw materials into 50 to 70 percent ethanol of which the weight is eight times that of ginkgo leaves serving as the raw materials to perform leaching three times, wherein the leaching temperature is 70 DEG C; collecting extracting solution, evaporating the extracting solution under vacuum, recovering ethanol, centrifuging the extracting solution and collecting supernatant solution; and subjecting the extracting solution to the centrifugal treatment by a macroporous resin chromatographic column of which the diameter-to-height ratio is 1:10, leaching the extracting solution in turn with 5 to 15 percent ethanol until the eluate is clear and bright, desorbing the extracting solution with 75 percent ethanol, after desorption, collecting desorbing solution, allowing the desorbing solution to pass through a macroporous resin chromatographic column of which the diameter-to-height ratio is 1:30, collecting the desorbing solution, decompression-recovering ethanol, condensing the desorbing solution into thick paste, and vacuum-drying the thick paste to obtain the ginkgo extract. The process adopts multiple leaching and multistage centrifuging, and also adopts a double-time desorbing method of coarse extraction and refined extraction, so that the contents of the active components are greatly improved; and the ginkgo extract with different ratios of active components can be prepared as required.

The invention belongs to the field of marine microalgae cultivation, and relates to a formula of a Chaetoceros muelleri medium and a white plastic barrel aerated culture method. Chaetoceros muelleri is a small marine diatom, rich in polyunsaturated fatty acid and protein, and is a high-quality bait for raising sea treasures such as prawn and abalone. At present, an f / 2 culture medium formula used to produce Chaetoceros muelleri has problems of imbalanced and incomprehensive nutrients, slow growth, long multiplication time and low yield. ccording to experiments, nitrate is substituted by urea, which is used as a nitrogen source and supplemented by NaHCO3 and growth hormone, VB12 and VB1, so that the medium gains more comprehensive and balanced nutrients, and yield of Chaetoceros muelleri is increased by 87%. In addition, cement pool and glass cylinder barrel used for culture in current production have many disadvantages: easy pollution, difficult washing, inconvenient operation and easy cutting. Study in the invention shows that a white plastic barrel aerated culture method for culturing Chaetoceros muelleri has advantages of flexible operation, no pollution, easy washing, no cutting, low cost, high yield and large-scale promotion.

The invention relates to a method for aligning section raw data on the basis of double time scale quantitative evaluation measurement point delay and performing prediction and modification. By the method, multi-source section raw data with different delay can be aligned. According to data delay differences among different regions, different stations and different transmission types, the method comprises the following steps of: quantitatively evaluating delay time of each measurement point by using sampling sequences with two time scales; predicting and modifying the current numerical value through an autoregression-sliding average model; evaluating the precision grade of the numerical value and a delay distribution interval; and providing for a state estimator. By the method, the data delay of different measurement points can be quantified accurately; the current section numerical value of the measurement point can be predicted and modified according to the delay; and precision weightis endowed, so that the aligned multi-source real-time data is integrated into a section closer to the operation condition of the current power grid.

The invention provides a stem cell culture medium and a method for culturing endometrium stem cells by using the stem cell culture medium. The method comprises the following steps: separately collecting menstrual blood and endometrium tissues, respectively culturing the menstrual blood and endometrium tissues in the stem cell culture medium provided by the invention to respectively obtain menstrual blood adherent cells and endometrium adherent cells, culturing the menstrual blood adherent cells and endometrium adherent cells in a cell culture bottle, collecting the adherent cells by trypsinization, inoculating the adherent cells in a cell coculture dish, and culturing the adherent cells in the stem cell culture medium provided by the invention. The stem cell culture medium has the advantages of simple components, fewer added components and lower cost. After more than 20 generations of in-vitro culture, the cells can not easily have the phenomenon of aging or degeneration, and can maintain the activity and stem property of the stem cells for a long time. The stem cell culture method is simple and effective, the cell proliferation efficiency is high, and the in-vitro culture doubling time is only 20 hours or so. The cells can be stably amplified by 50 generations.

The invention provides a serum-free culture medium used for full-suspension culture of MDCK (Madin Darby Canine Kidney) cells. The culture medium is prepared from a DMEM / F12 culture medium containing 5g / L of NaCl and the following components: sodium stannite, recombinant human epidermal growth factors, hydrocortisone, recombinant full-chain insulin, prostaglandin E1, human transferrin, thyroxine (T3), vitamin E, cholesterol, ethanol amine, beta-mercaptoethanol, Tween-80, Hypep1510, recombinant human serum albumin ACF and mannitol. With the adoption of the culture medium provided by the invention, the full-suspension culture of the MDCK cells can be carried out very well under the condition that serum is not added; after the MDCK cells are continuously cultured for 20 generations, the average proliferation concentration of the MDCK cells is 2.308*10<6> / mL, the average cell viability is 97.4 percent and the average doubling time is 34.48 hours, so that the serum-free culture medium provides technical supports for developing influenza vaccines of cell matrixes of mammals in China.

The invention discloses a method for producing glutamic acid through double-feeding fermentation optimization of corn steep liquor and glucose, which comprises the following steps of: in fermentation culture of glutamic acid, initially adding 1-10g / L of glucose to a fermentation culture medium, and initially adding 1-10g / L of corn steep liquor; after the fermentation culture starts, starting feeding the corn steep liquor and glucose at the same time, wherein 5-19g / L of corn steep liquor is fed and completely added within 5-10 hours since the fermentation starts, and the total amount of the corn steep liquor is 15-20g / L; feeding the concentrated solution of glucose until the fermentation is over; maintaining the amount of residual sugar in the fermentation culture medium at 0.05-10g / L; and controlling the pH value of the liquid ammonia in the whole process of fermentation to 6.8-7.3. The method disclosed by the invention effectively increases the growth speed of thalli and shortens the adaptation period in the initial stage of thallus fermentation and the thallus doubling time, thus shortening the overall fermentation time and reducing the overall production cost of glutamic acid.

The invention discloses an SOC and SOH joint estimation method, which comprises the following steps: obtaining a training sample set by using data measured by an experiment, wherein each training sample in the training sample set comprises average SOC, environment temperature, current, accumulated charge quantity and corresponding SOH; training a BP neural network to estimate SOH based on the training sample set; and estimating the SOC by using the UKF algorithm every first preset time based on the present SOH, and when the total time reaches second preset time, estimating the SOH by using theneural network on the basis of the environment temperature and current acquired by the current sensor and the SOC and accumulated charge quantity acquired by the SOC estimation algorithm, thereby realizing the joint estimation of the SOC and the SOH. According to the invention, the coupling relationship between the SOC and the SOH and the influence of various aging factors (such as temperature, current and the like) are fully considered in the estimation process of the SOC and the SOH; meanwhile, double-time estimation is adopted according to the change speed difference of the SOC and the SOH, the calculation efficiency is improved on the premise that the estimation precision is not reduced, and intelligent recognition can be well carried out.

A weighted fraction Fourier transform domain double-time-slot diversity and multiplexing cooperated transmission method belongs to the field of wireless communication technology. The method settles aproblem that diversity gain and multiplexing gain cannot be realized in an existing single-antenna system. The method utilizes double-time-slot expansion and weighted fraction Fourier technology. Through expanding a single-time-slot signal to double time slots, fraction domain calculation and transmission are successively performed, thereby realizing diversity gain and multiplexing gain in the single-antenna system. In the method of the invention, a receiving end can receive multiple data signals through de-multiplexing, thereby supplying higher user accommodation amount to the system, and realizing relatively high integral system performance. The method according to the invention can be applied to the field of wireless communication technology.

The invention relates to an intelligent desktop 3D printer based on the Internet of Things. The intelligent desktop 3D printer comprises a base, two stand columns, a cross beam and a printing mechanism, wherein the two stand columns are vertically arranged on the base and located at the two ends of the base; the cross beam is erected at the top ends of the stand columns; and the printing mechanism is arranged below the cross beam. It can be guaranteed that a user can remotely operate and control the intelligent desktop 3D printer based on the Internet of Things through a wireless communication module, so that intelligence of the printer is realized. Two feeding assemblies can conduct uniform and regular feeding through a driving wheel, and reliability and accuracy of material addition are realized, so that the printing reliability of the printer is ensured. Meanwhile, the feeding quantity can be further accurately controlled through a timing module, and accordingly the reliability of the printer is further improved. Besides, a double-time-base integrated circuit is adopted as a timing circuit and has the characteristics of stable performance and low price, so that the production cost of the printer is reduced, and the market competitiveness of the printer is improved.

The invention relates to a method for accurately estimating the Doppler rate in a large-strabismus SAR imaging mode. The method comprises the steps that 100) distance walk compensation, distance bending correction and secondary distance compression are implemented on echo data to obtain a double-time-domain signal which is compressed in the distance direction; 101) a dechirp processing is implemented on the distance compressed data in an orientation time domain by utilizing a reference function Sdechirp(t); 102) FFT (Fast Fourier Transform) is implemented on the orientation direction data to change the data from the orientation time domain to an orientation frequency domain; 103) a special display point is selected from each distance door in a maximal energy method, the orientation-direction position of the point is estimated, and a compensation function Scomp(t) of each special display point is calculated according to the orientation-direction position; 104) windowing is implemented, data within the main fuzzy width of the special display point selected from each distance door is reserved, and data which has no contribution to estimation of the Doppler rate is abandoned; 105) reverse Fourier transformer is implemented, and space change phase is compensated in the orientation time domain; and 106) the Doppler rate is estimated in a classic MD algorithm.

Compositions and methods for the culturing, propagation, cryopreservation and manipulation of neural progenitor cells (NPC) and pluripotent stem cells (PSC) are provided. The cells exhibit rapid doubling times and can be maintained in vitro for extended periods. Also provided is a method of propagating neural progenitor cells, and a method of transplanting human NPC and / or PSC to a host. The cells can be genetically modified to express a therapeutic agent prior to the transplanting.

A method and apparatus is provided for clamping of an electrostatic discharge using multiple time-constants. A rise in a voltage level is detected. A first time-constant is provided to determine whether the rise in the voltage level is caused by an electrostatic event. A clamping function is provided to clamp the voltage level in response to a determination that the rise in the voltage level is caused by the electrostatic event. A second time-constant is provided to provide a time period for maintaining the clamping function for a period of time to substantially extinguish the electrostatic event.

The invention discloses a high voltage NMOS using the tagma double-times doping way, wherein the second time doping is performed by the self-alignment and the step phenomenon in the channel is eliminated and the device performance is increased.

A method and apparatus is provided for clamping of an electrostatic discharge using multiple time-constants. A rise in a voltage level is detected. A first time-constant is provided to determine whether the rise in the voltage level is caused by an electrostatic event. A clamping function is provided to clamp the voltage level in response to a determination that the rise in the voltage level is caused by the electrostatic event. A second time-constant is provided to provide a time period for maintaining the clamping function for a period of time to substantially extinguish the electrostatic event.

A method and composition for preventing prostate cancer and / or reducing PSA levels and / or alleviating the symptoms of BPH (Benign Prostatic Hyperplasia) or PIN (prostatic intraepithelial neoplasia) by administration of an effective amount of a mixture of flavopereirine and alstonine. A method and composition for treating low-grade prostate cancer and preventing the onset of metastatic disease and / or reducing the doubling time of PSA levels in men with positive biopsies showing relatively low Gleason scores and morphologies characteristic of non-invasive, slow-progressing prostate cancer. The flavopereirine and alstonine can be in the form of natural extracts derived from Pao Pereira and Rauwolfia Vomitoria, respectively. Alternatively, these two active compounds can be administered in purified form. The composition can be in included in a kit along with instructions for use in a treatment regimen.

The invention discloses a processing method of camellia red tea and relates to the field of tea processing. The processing method comprises the following steps: picking fresh leaves and camellia; withering the fresh leaves and the camellia in a withering tank; rolling the withered leaves and the withered camellia in a rolling machine; fermenting in a fermentation room; drying the fermented tea and camellia in a drier; finally flavoring the dried tea and camellia in a flavoring machine. According to the processing method disclosed by the invention, three times of flavoring and double times of cooling are adopted especially in the flavoring step, so that the processed red tea is red, transparent and bright in tea soup, has the fragrance of the camellia and is mellow, normal and thick in taste.

The present invention is double time phase protamine zinc insulin injection (30 %) and its preparation process, and belongs to the field of medicine preparation technology. The double time phase protamine zinc insulin injection (30 %) consists of insulin 100000 IU, isosmotic agent 35-45 g, preservative 5.5-7 g, pH regulator 12-12.5 g and fish protamine sulfate 0.21-0.42 g, and has pH value of 6.9-7.8. Its preparation process includes the steps of preparing neutral insulin injection, preparing low protamine zinc insulin injection, and mixing these two kinds of injection. The double time phase protamine zinc insulin injection (30 %) has two forms of insulin, including one in solution for quick acting and the other in precipitate with combined fish protamine sulfate as the middle acting phase. It has less required injection times.

The invention discloses a networked multi-sensor fusion fault detection method, and belongs to the field of networked systems. The method comprises the steps of firstly establishing a double-time-scale system model with parameter perturbation and faults, solving the data communication problem of a shared network through a weighted Try-Once-Discard protocol, designing a local fault detection filterand a global fusion fault detection filter, and introducing a residual evaluation mechanism to judge whether the system has faults or not; applying a Lyapunov stability theory and an LMI technology to obtain the sufficient conditions of the local fault detection filter and the global fusion fault detection filter; and using an LMI toolbox to solve the optimization problem, and obtaining a local optimal fault detection filter parameter and a global optimal fusion weight parameter. According to the method, the parameter perturbation, network bandwidth limitation and faults of the system under the actual condition are considered, and the method is suitable for the fault detection of a general networked system and has better universality.

The invention discloses an antenna complex weight feeding network based on double time modulation, which comprises N double time modulation complex weight feeding units, an FPGA control circuit and 1-N power divider. The double time modulation complex weight feeding unit is used for completing the single sideband amplitude and phase weighting of the network; The FPGA control circuit is used for issuing a periodic time series to control the double time modulation complex weight feeding unit; and 1-N power divider is used for feeding the N double time modulation complex weight feeding units. Theinvention is based on the time modulation technology, and periodically sends out a control signal to change the state of a radio frequency switch through the FPGA circuit, so that the antenna can simultaneously complete the amplitude and phase weighting of the feeding network without the variable gain amplifier and the phase shifter.

The invention discloses a multi-purpose water-containing granular air purifying agent and a production method thereof. The multi-purpose water-containing granular air purifying agent consists of purified water, a water storage agent, a composite slow-release agent, an adsorbing agent, a composite disinfector, a plant aroma purifying agent, an oxygen release agent and a plant binding agent, wherein the water storage agent is expanded perlite, the composite slow-release agent is propylene glycol, glycerol and 1,3-butanediol, the composite adsorbing agent is activated carbon and diatom mud, the composite disinfector is chlorine dioxide and sodium trichlorosiocyanurate, the composite aroma purifying agent is peppermint oil, lemon oil, Chinese mugwort leaf essential oil, rosemary essential oil and sweet-scented osmanthus essential oil, the oxygen release agent is sodium percarbonate, and the plant binding agent is guar gum. A product disclosed by the invention has the characteristics that the product is high in purification degree, can be used for effectively prolonging the use time for double times compared with a conventional product, is low in investment, low in cost and easily-purchasable in raw material, has a large amount of adsorbing effective liquid materials by finished product particles, cannot cause three wastes, is high in air purification degree, and has multiple effects on oxygen release, sterilization, purification and the like, and can be applied in rooms or vehicles.

The invention relates to the field of wireless communications, and discloses a transmission strategy that fully utilizes double time slots to transmit information, and a multi-resource joint optimization algorithm in a DF (Decode-and-Forward) cooperative multi-relay OFDM (Orthogonal Frequency Division Multiplexing) system under the transmission strategy. The multi-resource joint optimization method comprises the following steps: considering a time-division half-duplex relay, and sending information by a source node on each subcarrier in two time slots; and under a total power constraint condition, performing joint optimization on multi-relay selection and cooperative transmission, time slot freedom, transmission mode, subcarrier pairing and power allocation and other resources, so as to maximize the system transmission rate. The whole system structure is composed of one source node, one target node and K relay nodes, and a selective transmission bandwidth is divided into N subcarriers through an OFDM technology. Compared with the prior art, the optimization algorithm of the invention has a higher information transmission rate and spectral efficiency.