266 results about "Protamine" patented technology

Methods and devices for point of care determination of heparin concentration in blood are described. Cartridges including protamine ion sensitive electrodes (ISEs) and reference electrodes and systems for automatically determining heparin concentration in the cartridges are provided. Some systems add blood to a protamine bolus sufficient to bind all heparin, leaving excess protamine. The excess protamine concentration can be determined by measuring the initial slope of the electrode potential rate of change, and comparing the slope to known protamine concentration slope values In some cartridges, an oscillating pressure source moves the blood-protamine mixture back and forth across the protamine ISE. Some systems also use a second blood sample having the heparin removed or degraded to create a blank reference sample. Protamine ISEs can include polyurethane polymer, DNNS ionophore, and NPOE plasticizer. The polyurethane may include hard segments and soft segments, where both hard and soft segments may include cyclic and straight chain aliphatic moieties having essentially no ester or ether groups. Some hard segments may include methylene diphenyl groups. Some reference electrodes have the same polymer, plasticizer, and ionophore as the measurement electrode, but with a different concentration of ionophore.

Described herein are compositions and methods of use of targeted delivery complexes for delivery of siRNA to a disease-associated cell, tissue or pathogen. The targeted delivery complex comprises a targeting molecule, such as an antibody or fragment thereof, conjugated to one or more siRNA carriers. In preferred embodiments the siRNA carrier is a dendrimer or protamine and the targeting molecule is an anti-cancer antibody, such as hRS7. More preferably, the antibody or fragment is rapidly internalized into the target cell to facilitate uptake of the siRNA. Most preferably, the targeted delivery complex is made by the DNL technique. The compositions and methods are of use to treat a variety of disease states, such as cancer, autoimmune disease, immune dysfunction, cardiac disease, neurologic disease, inflammatory disease or infectious disease.

The invention discloses a swelling granular mixed feed for adult pelteobagrus fulvidraco, relating to the field of pelteobagrus fulvidraco feed. The feed is suitable for feeding the pelteobagrus fulvidraco in adult stage (namely the pelteobagrus fulvidraco in the adult stage with the weight of more than 20g); the feed is a mixture prepared by all raw materials according to the processing method of swelling granular feeds; according to the weight percentage, the feed comprises the following raw materials of: 36-40% of fish meal, 15.58-19.6% of flour, 3.5-4% of cottonseed meal, 16-20% of soybean meal, 6-7% of maize protamine, 4.5-5.5% of fish oil, 6-7% of beer yeast, 1-1.5% of mineral matters, 0.5-0.6% of choline chloride and 0.2% of multivitamin. Compared with normal mixed feed, the swelling granular mixed feed has the advantages of comprehensive nutrition, high livability, little pollution on culture water, greenness, and no public nuisance.

Disclosed is a pharmaceutical composition for gene delivery, comprising: (i) a gene; (ii) a water-soluble chitosan; (iii) a thiamine pyrophosphate or a pharmaceutically acceptable salt thereof; (iv) a protamine or a pharmaceutically acceptable salt thereof; and (v) a neutral or anionic phospholipid. The composition can introduce a gene into cells safely and effectively. Composed of non-toxic and injectable components, the composition is safe for the body and can be advantageously commercialized. Notably, it can deliver a gene at high efficiency in vivo as well as in vitro, and is stable in the blood.

Described herein are compositions and methods of use of targeted delivery complexes for delivery of siRNA to a disease-associated cell, tissue or pathogen. The targeted delivery complex comprises a targeting molecule, such as an antibody or fragment thereof, conjugated to one or more siRNA carriers. In preferred embodiments the siRNA carrier is a dendrimer or protamine and the targeting molecule is an anti-cancer antibody, such as hRS7. More preferably, the antibody or fragment is rapidly internalized into the target cell to facilitate uptake of the siRNA. Most preferably, the targeted delivery complex is made by the DNL technique. The compositions and methods are of use to treat a variety of disease states, such as cancer, autoimmune disease, immune dysfunction, cardiac disease, neurologic disease, inflammatory disease or infectious disease.

The invention discloses a process for making low-temperature vacuum fried anchovy. The invention effectively solves the problem that an anchovy product is hard in meat quality, insufficient in crisp degree and not easy to chew because the anchovy is easy to rot and deteriorate in a processing procedure. The process comprises the following steps of: (1) raw material preprocessing; (2) deodorization; (3) dipping; (4) baking at a low temperature; (5) freezing; (6) frying in vacuum; and (7) deoiling. In the dipping step of the process, additives composed of 30-35% of dehydrated celery powder, 2-5% of protamine, 20-30% of microcrystalline cellulose, 30-35% of methylcellulose, 2-5% of sodium hydrogen sulfite and 3-8% of vitamin C by weight percentage are added. The process provided by the invention is simple and practicable, convenient to operate and suitable for factorized production and application; the anchovy in the making process is unlikely to rot and deteriorate; and the low-temperature vacuum fried anchovy obtained through the process provided by the invention is less in fishy smell, good in color and luster, crisp in taste, low in oil content and low in nutrition loss.

The invention discloses a natural fresh-keeping antiseptic containing protamine. The invention is characterized in that the antiseptic is prepared from the following raw materials by weight ratio: 0.3-0.5 parts of the protamine, 1-1.5 parts of carboxymethyl chitosan, 1.2-1.8 parts of tea polyphenol and 0.6-0.8 parts of oxalic acid. The antiseptic overcomes limitations that present natural antiseptic is difficult to effectively maintain freshness and prolong preservation time; through effective combination of the protamine with the tea polyphenol and the carboxymethyl chitosan, the antiseptic can both significantly prolong preservation time of food and improve freshness of preserved food, and the antiseptic is safe, reliable, and easy for large-scale promotion.

The invention discloses a swelling granular mixed feed for parr of pelteobagrus fulvidraco, relating to the field of pelteobagrus fulvidraco feed. The feed is suitable for feeding the pelteobagrus fulvidraco in parr stage (namely the pelteobagrus fulvidraco in the parr stage with the weight of 2-20g); the feed is a mixture prepared by all raw materials according to the processing method of swelling granular feeds; according to the weight percentage, the feed comprises the following raw materials of: 44-48% of fish meal, 15.05-19.55% of flour, 2-5% of cottonseed meal, 15-17% of soybean meal, 3.5-5% of maize protamine, 3.5-4.5% of fish oil, 5-6% of beer yeast, 1-1.2% of mineral matters, 0.5-0.6% of choline chloride and 0.2% of multivitamin. Compared with normal mixed feed, the swelling granular mixed feed has the advantages of comprehensive nutrition, high livability, little pollution on culture water, greenness, and no public nuisance.

The invention provides an environment-friendly and non-toxic preservative for condiments and a preparation method of the preservative and relates to the technical field of food additives. The preservative is prepared from the following raw materials: protamine, chitosan, a tea polyphenol extract, a Chinese herbal medicine extract, trans-cinnamic acid, agaro-oligosaccharides, bacteriocin, nisin, a spice extract, an acidulant, ethanol and water. The prepared preservative is environment-friendly, non-toxic and pure-natural, compounds a plant source, an animal source and a microorganism source, is non-toxic and harmless for a human body, cannot affect human health, can effectively prolong the shelf life of a hot sauce product and has very high sterilization and bacteriostasis capabilities.