The invention discloses a method for preparing
cysteine-
histidine dipeptide /
copper ion coordination compound nano-
enzyme (CH-Cu). The method comprises the following steps: 1) weighing 20-120 mg of
cysteine-
histidine dipeptide powder, dissolving the
cysteine-
histidine dipeptide powder in 0.5-6 mL of deionized water, and carrying out ultrasonic treatment at
room temperature for 10-60 min; 2) weighing 10-120 mg of soluble
copper salt, dissolving the soluble
copper salt in 0.5-5 mL of deionized water, and performing ultrasonic treatment at
room temperature for 10-60 min; 3) mixing the cysteine-histidine dipeptide solution obtained in the step 1) and the
copper salt solution obtained in the step 2) with 2-8 mL of N,N-
dimethylformamide (DMF), and performing ultrasonic treatment for 10-60 minutes; and 4) transferring the
mixed solution obtained in the step 3) into a pressure-resistant tube or a hydrothermal kettle, carrying out a reaction for 2-8 h at a temperature of 80-140 DEG C, then carrying out centrifugal collection, and respectively carrying out cleaning three times with N,N-
dimethylformamide and deionized water so as to obtain cysteine-histidine dipeptide and copper
ion coordination compound nano-
enzyme particles with a particle size of 30-200 nm. The invention also discloses application of the prepared nano-
enzyme in degradation of 2,4-
dichlorophenol,
hydroquinone and
epinephrine.