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Culture medium and culture method for Dicrateria zhanjiangensis

A culture method and medium technology, applied in the field of microalgae cultivation, can solve the problems of long doubling time, slow growth rate, and troublesome operation of algal cells, and achieve the effects of shortened culture period, slow growth rate, and easy operation.

Active Publication Date: 2014-05-21
江苏津沂菊源生物健康产业研究院有限公司
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Problems solved by technology

[0005] The cultivation of Chrysoflagellate Zhanjiang traditionally uses f / 2 medium and open cement pond culture, the algal cell doubling time is long, the growth rate is slow, the investment is large, the yield is low, easy to pollute, and the operation is troublesome

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  • Culture medium and culture method for Dicrateria zhanjiangensis

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Embodiment Construction

[0020] 1) Chrysoflagellate zhanjiang used for inoculation is pre-purified (conventional methods in the art, as long as purification can be achieved, such as Soxhlet extraction method), to ensure that the algae species are in the exponential growth phase during inoculation;

[0021] 2) 920 plant growth stimulant is a white crystalline powder, which is not easy to dissolve in water. It can be fully dissolved in alcohol first (generally dissolve with 50 ml of alcohol or 60% white wine for more than 1 hour per gram), and then add water according to the required concentration dilution;

[0022] 3) Due to iron citrate (FeC 6 h 5 o 7 ·5H 2 O) It is difficult to dissolve, you can add a small amount of tap water and heat it slightly to 80-90°C on the stove, and keep stirring until it is completely melted;

[0023] 4) Various nutrient salts should be added in the order provided in the formula to avoid chemical reactions;

[0024] 5) All kinds of nutrient salts, especially f / 2 trace...

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Abstract

The invention belongs to the field of microalgae culture and discloses a formula of a Dicrateria zhanjiangensis improved culture medium and a simple culture technique of Dicrateria zhanjiangensis. Dicrateria zhanjiangensis is traditionally cultured by using an f / 2 culture medium in an open cement pit manner, so the doubling time of algae cells is long, the grow speed is low, the investment is high, the yield is low, pollution is easily caused, and the operation is troublesome. When Dicrateria zhanjiangensis cells are cultured by using the culture medium provided by the invention and mineral water barrels, the content of unsaturated fatty acid is high, the cost is low, pollution is avoided, the operation is easy, and the workload is greatly reduced; and nutrients such as sodium hydrogen carbonate, ferric citrate, potassium sorbate, glucose, sea mud extract, soil extract, ethylenediamine tetraacetic acid complexing agent and cow dung and goat manure extract are added in the culture medium, and organic fertilizers and inorganic fertilizers are used together, so that more nutrients are available, the growth speed of the Dicrateria zhanjiangensis is greatly improved, the culture period is shortened, and the yield is improved by 92%.

Description

technical field [0001] The invention belongs to the field of microalgae culture, and in particular relates to a culture medium and a culture method of Chrysopsis zhanjiang. Background technique [0002] Dicrateria zhanjiangensis belongs to Chrysophyta Chrysophyta, Chrysomonadales, Isochrysidaceae, Dicrateria, is a planktonic unicellular algae with a size of 2-7 microns. Because it has no cell wall, is rich in unsaturated fatty acids, has strong ecological adaptability, fast growth, and is suitable for large-scale reproduction, it is considered to be an ideal bait for artificial breeding of marine animals. Excellent opening feed for aquatic products. [0003] Inorganic carbon in seawater exists in the form of HCO 3 - Mainly (90%), while free CO 2 Less than 1%. In the past, it was believed that free CO in water 2 It is the only form of exogenous inorganic carbon used by algae. Zhang Xuecheng's work showed that adding 50-100mmol / L NaHCO to the Spirulina medium 3 , not o...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 王培磊
Owner 江苏津沂菊源生物健康产业研究院有限公司
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