51results about How to "Short doubling time" patented technology

The present invention belongs to the technique category of the medical instrument, relates to a tectorial conformal radiate NiTi alloy vascular inner rack which is implanted to the blood vessel of the human body. To settle the problems of that the existing vascular inner rack can not restrain the growth of the tumour and the thrombus is easy to form, etc., the bracket used by the invention is self-expanding NiTi alloy vascular inner rack, a layer of polycarbonate type polyurethane membrane is covered at the inner wall, the antineoplastic medicine is spraying-coated to the inner surface of the membrane, and the active particles which are used for the inner radiating of the tumour are inserted at the outer bracket of the membrane according to the conformal property. The NiTi alloy vascular inner rack applied by the invention can effectively expand and support the blood vessel; the novel film forming material polycarbonate type polyurethane has an excellent bioavailability; the active particles for the conformal radiotheraphy outside the membrane are inserted to the bracket with a simple and convenient double-buckle mode, the growth surround the blood vessel area is effectively restrained and the radioactive side injury of the normal tissue is reduced. The invention has the advantages of easy making, low cost, strong innovation and wide market prospect.

The invention provides cell culture fluid of serum-free cultivation for enhancing immunosuppression function of animal mesenchymal stem cells. Multiplication factors and specific additives are added into a basic nutrient solution for serum-free cultivation of animal mesenchymal stem cells, in order to promote healthy growth and proliferation of animal mesenchymal stem cells, and provide functions for maintaining and enhancing immunosuppression of animal mesenchymal stem cells. The fluid has the function for enhancing function of animal mesenchymal stem cells. Experiments prove that the serum-free culture fluid for enhancing immunosuppression function of animal mesenchymal stem cells can be used for serum-free cultivation of animal mesenchymal stem cells, and can be applied to clinic research and application in the aspect of immunosuppression application research of mesenchymal stem cells.

The invention discloses an shRNA (Short Hairpin Ribonucleic Acid) molecule of a PC9 cell strain knocked down by a TAZ factor, a recombinant vector of the shRNA molecule, the recombinant vector of the PC9 cell strain over-expressed by the TAZ factor, and an establishment method of the PC9 cell strain knocked down and over-expressed by the TAZ. The method comprises the following steps: establishing the PC9 cell strain knocked down by the TAZ by transfecting PC9 cells via TAZ-shRNA by adopting an shRNA technology; establishing the PC9 cell strain over-expressed by the TAZ by transfecting the PC9 cells via a TAZ expression vector; detecting the TAZ in the cell strain by adopting an immune cell fluorescence technology, a RT-PCR (Reverse Transcription-Polymerase Chain Reaction) technology and a Western blot technology so as to prove the successful establishment of the cell strain; and measuring a cell growth curve and the cell doubling time. Thus, the powerful experimental material for researching the functions of the TAZ in the targeted therapy sensitivity of lung cancers is provided on the basis of the establishment of the PC9 cell strain knocked down and over-expressed by the TAZ.

The invention relates to a domesticated CHO-S cell system and a culture method and application thereof. The domesticated CHO-S cell system is obtained by passing CHO-S cells for at least 5 generations through a specially formed XH001 culture medium by a gradual domestication method. The invention also discloses a domestication method and application of the domesticated CHO-S cell system. The domesticated CHO-S cell system has the characteristics of high growth speed, short multiplication time, good cell form, uniform shape and the like. When the domesticated CHO-S cell system is used for recombinant antibody expression, the expression quantity can be obviously improved.

The invention provides a culture medium for in-vitro amplification of autologous human hair follicle mesenchymal stem cells and a culture method of the culture medium, and belongs to the technical field of cell culture. The culture medium for in-vitro amplification of the autologous human hair follicle mesenchymal stem cells takes DMEM / F-12 as a basic culture medium and is prepared from the following components in percentage by volume: 4 to 6 percent of autologous serum, 9 to 11ng / ml of bFGF and 0.8 to 1.2 percent of penicillin-streptomycin. According to the culture method, human autoserum isused as an additive to prepare an amplification culture medium, and in-vitro large-scale amplification of autologous human hair follicle mesenchymal stem cells is carried out. Compared with the traditional mesenchymal stem cells, the hair follicle mesenchymal stem cells cultured by the method have the advantages that other animal serum or allogeneic blood products are not required to be added, thesafety is high, the allogeneic disease propagation risk is avoided, the cell proliferation rate is high, and the characteristics of the mesenchymal stem cells can still be maintained after multiple passages.

The invention belongs to the field of environmental engineering, which relates to an integrated expanded granular sludge bed-membrane bioreactor whole-course autotrophy denitrification device and a process thereof. The device comprises a reactor main body, an air / liquid separation tank, a three-phase separation device, a membrane assembly, an air pump, an air flow meter, a perforated aeration pipe, a water feeding pump, a reflux pump, a water discharging pump and the like, wherein the reactor main body is divided into two function regions, i.e., a lower whole-course autotrophy denitrification function region and an upper membrane filtration function region, wherein an air aeration mode is adopted by the bottom of the immersion-type membrane assembly in the upper membrane filtration function region; the upper part of the reactor is externally connected with the air / liquid separation tank; the reflux system is used for refluxing heavy-oxygen-enriched water in the tank to a water inlet in the bottom of the reactor so as to provide the necessary dissolved oxygen for the whole-course autotrophy denitrification function region; and the fed sewage is firstly denitrified by the whole-course autotrophy denitrification function region and then filtered by the membrane so as to obtain the clean discharging water. The integrated reactor is small in land occupation. The whole-course autotrophy denitrification process is simple and convenient to operate.

A serum-free C3A clonal cell line and methods for generating the same are provided. The C3A cell line has a reduced doubling time in serum-free medium compared to a corresponding C3A cell line from which it is derived. Methods using the cells of the serum-free C3A clonal cell line for the production, expression and recovery of harvestable polypeptides, screening compounds for metabolic activity, studying enteric disease and for use in a bio-artificial liver device are also provided.

The present invention discloses a low-serum culture medium suitable for BHK-21 cell flask rotation culture, and a preparation method thereof. According to the present invention, in the flask rotation adherence culture, the serum consumption is substantially to 1% from the conventional 10%, the cell growth rate is rapid, the achieved cell density is high, and the high vitality is maintained; and during the culture medium development process, a plurality of compounds having special functions, lipid components, yeast, plant hydrolyzates and recombinant proteins are used to replace the conventionally-added animal-derived components in the culture medium, hormones, lipids, trace elements, hydrolyzates and other components are added to replace the partial functions of the serum so as to substantially reduce the serum addition amount, and reduce the serum cost while reduce the probability of the pollution caused by exogenous pathogenic bacteria carried by the serum and the animal-derived proteins in the culture medium, and the protein content in the culture medium is substantially reduced, such that the purification process at the late stage is easily achieved, and the stability of the cell product is improved.