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Hyaluronic acid method for promoting proliferation of human amniotic membrane stem cells and application thereof

A technology of stem cell proliferation and hyaluronic acid, applied in the field of pharmaceuticals, can solve the problems of high-efficiency expansion, limitation, and high price of hASCs that have not been seen in vitro

Active Publication Date: 2016-08-17
AFFILIATED HOSPITAL OF ZUNYI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These exogenous factors that can promote the proliferation of mesenchymal stem cells have greater risks in their clinical application, for example, safety issues such as immunogenicity and chemical toxicity. In addition, many factors are extremely expensive, which limits their clinical application.
In addition, there are no relevant reports on the high-efficiency expansion of hASCs in vitro

Method used

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  • Hyaluronic acid method for promoting proliferation of human amniotic membrane stem cells and application thereof
  • Hyaluronic acid method for promoting proliferation of human amniotic membrane stem cells and application thereof
  • Hyaluronic acid method for promoting proliferation of human amniotic membrane stem cells and application thereof

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Embodiment 1

[0077] Embodiment 1 of the present invention: a method for promoting the proliferation of human amniotic membrane stem cells by hyaluronic acid, collecting the cultured second-generation human amniotic membrane epithelial cells, seeding them in 96-well plates with 3000-5000 cells / well, and replacing them after 24 hours L-DMEM full medium; then add hyaluronic acid to the culture medium to make the concentration of hyaluronic acid reach 0.01-20 mg / mL; place the culture plate in saturated humidity, 5% CO 2 In an incubator, culture at 37° C. for 12-168 h and stop; the molecular weight of the hyaluronic acid is 20-2200 kDa.

Embodiment 2

[0078] Embodiment 2 of the present invention: the application of hyaluronic acid in the preparation of a drug for promoting the proliferation of human amniotic membrane stem cells. Hyaluronic acid can be used alone, or can be combined with cell growth factors to make powder or water according to conventional methods.

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Abstract

The invention discloses a hyaluronic acid method for promoting proliferation of human amniotic membrane stem cells and application thereof. The present invention applies hyaluronic acid in drugs for promoting proliferation of human amniotic stem cells. Through a series of tests, it is fully proved that hyaluronic acid can significantly reduce the doubling time of human amniotic stem cells (hASCs) and promote hASCs proliferation, has safety, and does not affect hASCs multi-differentiation potential of stem cells. Hyaluronic acid can be used alone, or a composition containing hyaluronic acid can be used for promoting the hASCs proliferation or manufacturing pharmaceuticals for the same purpose. The present invention has the advantages of wide range of sources, low cost, and side effects.

Description

technical field [0001] The invention relates to the field of pharmacy, in particular to a method for promoting the proliferation of human amniotic stem cells by hyaluronic acid and its application. Background technique [0002] Hyaluronic acid, also known as hyaluronic acid or hyaluronic acid, the English name is hyaluronan or hyaluronate or hyaluronic acid (HA), which is composed of glucuronic acid and N - Acetylglucosamine as a disaccharide unit via -1,4-glycosidic bond and -A chain polymer acidic mucopolysaccharide formed by alternating 1,3-glucosidic linkages, with a molecular weight ranging from 5 to 20,000kDa. In nature, hyaluronic acid is synthesized by hyaluronic acid synthase, widely distributed in the extracellular matrix of animals and human tissues (such as skin, cartilage, eye vitreous and microbial extracellular capsule, etc.), with its unique Molecular structure and physicochemical properties show a variety of important physiological functions in the body...

Claims

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Application Information

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IPC IPC(8): C12N5/074
Inventor 肖建辉钟建江刘如明孙仁刚陈代雄
Owner AFFILIATED HOSPITAL OF ZUNYI UNIV
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