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Stem cell medium and application thereof

A culture medium and stem cell technology, applied in the field of stem cell culture, can solve the problems of difficult stem cell culture and reduced differentiation potential.

Inactive Publication Date: 2014-07-16
苏州博棠再生医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a stem cell culture medium and its application, aiming to solve the problem that stem cell culture is difficult and its differentiation potential is reduced due to backward stem cell culture methods

Method used

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  • Stem cell medium and application thereof
  • Stem cell medium and application thereof
  • Stem cell medium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0196] Example 1: Experiments on Acceleration of Stem Cell Proliferation

[0197] 1. Test medium: conventional medium components: DMEM / F12 basic medium, 10% fetal bovine serum (FBS); the medium of the present invention.

[0198] 2. Source of cultured stem cells: human stem cells (ADSC, BMSC, UMBSC) derived from fat, bone marrow and umbilical cord respectively.

[0199] 3. In vitro cell culture experiments. The cell seeding density was controlled between 10-20%, and culture medium was added after seeding (37°C, 5% CO2). Cell density was measured every 12 hours by the MTT method. Figure 1-3 As the experimental results of this example, it can be seen from the figure that the stem cells (solid column) cultured in the medium of the present invention show a faster expansion rate than the cells (hollow column) cultured in the conventional medium , [data are the mean ± SEM of three independent experiments (P <0.0001)]. Therefore, compared with the conventional culture medium, the...

Embodiment 2

[0200] Example 2: Influence experiment on the potential analysis of proliferating mesenchymal stem cells divided into chondrogenic cells in vitro

[0201] 1. Test medium: conventional medium components: DMEM / F12 basic medium, 10% fetal bovine serum (FBS); the medium of the present invention.

[0202] 2. Source of cultured stem cells: human stem cells (ADSC, BMSC, UMBSC) derived from fat, bone marrow and umbilical cord respectively.

[0203]3. In vitro cell culture experiments. The chondrogenic differentiation experiments of human stem cells (ADSC, BMSC, UMBSC) derived from fat, bone marrow, and umbilical cord tissues and organs after expansion were determined according to the method described in the literature (Nat Protoc 2010; 5: 1294–1311; Biomaterials. 2004 Jul;25(16):3211-22; Tissue Eng Part A. 2009 Feb;15(2):231-41). When the stem cells grow to a suitable density (cell density 20-80%), add chondrogenic induction medium (DMEM / F12 medium with 1% FBS, 10 mg / L TGF, 50 nM vi...

Embodiment 3

[0204] Embodiment 3: Effect experiment on the potential analysis of adipogenic cells

[0205] 1. Test medium: conventional medium components: DMEM / F12 basic medium, 10% fetal bovine serum (FBS); the medium of the present invention.

[0206] 2. Source of cultured stem cells: human stem cells (ADSC, BMSC, UMBSC) derived from fat, bone marrow and umbilical cord respectively.

[0207] 3. In vitro cell culture experiments. In order to further examine the influence of stem cells on their differentiation potential after being amplified by the medium described in the present invention for 8 generations, we measured the The method described in the literature measured the differentiation experiment of adipocytes after the expansion of human stem cells (ADSC, BMSC, UMBSC) derived from fat, bone marrow and umbilical cord (Methods in Molecular Biology, 2011, Volume 702, Part 3 , 193-200; Methods. Volume 45, Issue 2, June 2008, Pages 115–120; Cytotherapy. 2003, Vol.5, No.5, Pages 362-369)...

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Abstract

The invention discloses a stem cell medium and an application thereof. The medium comprises a basic medium, fetal bovine serum, cytokine or protein polypeptide, vitamin and lipid. The medium provided by the invention can quickly amplify stem cells and does not influence the potential of the stem cells, and the amplification velocity of the stem cells is improved by 3-5 times than a conventional medium; and moreover, the medium can be used for culturing the stem cells of various tissues and has excellent applicability, the cultured stem cells have strong differentiation capability and can be differentiated into cells with various functions; therefore, the stem cell medium has high scientific and research and medical application values.

Description

technical field [0001] The invention relates to the technical field of stem cell culture, in particular to a stem cell culture medium and its application. Background technique [0002] Many studies have shown that stem cells can not only self-renew, but also differentiate into other functional cells under suitable conditions. Therefore, stem cells are expected to become an effective means of treating difficult human diseases. However, the content of stem cells in normal adult tissues is very small. How to rapidly expand and cultivate stem cells in vitro is an important technology for studying the mechanism of stem cells and exploring their therapeutic methods in the treatment of human diseases. [0003] The content of stem cells is as small as possible, but they are widely distributed in various tissues and organs of mammals, including but not limited to bone marrow, umbilical cord, adipose tissue, brain tissue, retina, heart, liver, lung and skin. A large number of studies...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775C12N5/0789C12N5/074C12N5/071
Inventor 刘小青
Owner 苏州博棠再生医学科技有限公司
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