180 results about "Human platelet" patented technology

Methods for inducing calcium mobilization in cells through the application of nanosecond pulsed electric fields (“nsPEFs”) are provided. The invention also provides a method of increasing intracellular calcium in cells through the application of nsPEFs. In one embodiment of the invention, the cells are human platelets, whereby activation and aggregation of the platelets is induced. Methods for treating an injury, trauma, or loss of blood in a subject, through the application of nsPEFs are also provided.

The invention discloses a biologically-safe quality control material for a blood tester and a preparation method thereof. The preparation method comprises that animal red blood cells having the sizes similar to sizes of human red blood cells are used for simulation of human red blood cells; animal red blood cells having the sizes similar to sizes of large cells in human white blood cells and animal red blood cells have the sizes similar to sizes of small cells in human white blood cells are used for simulation of human white blood cells; animal red blood cells have the sizes similar to sizes of human blood platelets are used for simulation of human blood platelets; the animal red blood cells for simulation of human white blood cells and human blood platelets are cured by formaldehyde having the content of 2-8%; the cured animal red blood cells are cleaned and then are added with bovine serum albumin so that the cure agent is removed; and according to requirements, through blending, the single-component or whole blood quality control material is obtained. The biologically-safe quality control material has good stability, a low cost and high safety, and is suitable for a blood cell analyzer, a urinary sediment analyzer and a hemoglobin analyzer.

The invention discloses a serum-free medium of a stem cell, which comprises a basal medium and an additive, wherein the basal medium is DMEM / F12 (Dulbecco Modified Eagle Medium / F12); the additive comprises 2-15%(v / v) of serum replacement, 20-100ug / ml vitamin C, 0.5-10ng / ml stem cell growth factor, 5-20ng / ml human platelet-derived growth factor and 1-5mmol / ml L-glutamine. The serum-free medium of the stem cell for cell culture has the advantages of short cell cycle, strong multiplication capacity, good cell uniformity and high purity, effectively inhibits differentiation of the stem cell and adherence growth of an endothelial cell, ensures purity and dryness of the stem cell and is free from ingredients of animal origin such as fetal calf serum, and the stem cell obtained by the medium is suitable for clinical application.

This invention provides an agonist antibody to a human thrombopoietin receptor (alias: human c-Mpl). More particularly, this invention provides an agonist antibody to a human thrombopoietin receptor, wherein the agonist antibody comprises: antibody constant regions comprising (1) amino acid sequences in a heavy chain constant region and a light chain constant region of a human antibody, (2) an amino acid sequence of a heavy chain constant region with a domain substituted between human antibody subclasses, and an amino acid sequence of a light chain constant region of a human antibody, or (3) amino acid sequences comprising a deletion(s), substitution(s), addition(s), or insertion(s) of one or several amino acid residues in the amino acid sequences of (1) or (2) above; and antibody variable regions capable of binding to and activating a human thrombopoietin receptor; and wherein the agonist antibody has the properties: (a) that the antibody induces colony formation at a concentration of 10,000 ng / ml or lower as determined by the CFU-MK colony formation assay using human umbilical-cord-blood-derived CD34+ cells; and (b) that the antibody has a maximal activity at least 50% higher than that of PEG-rHuMGDF and an 50% effective concentration (EC50) of 100 nM or less in the cell proliferation assay using UT7 / TPO cell. Also provided is a pharmaceutical composition for treating thrombocytopenia comprising said antibody.

The invention provides preparation of human platelet-rich plasma and application of the same in isolation and culture of human mesenchymal stem cells. According to the invention, erythrocytes and a mixture of leucocytes and platelets are separated from human whole blood through centrifugation, then centrifugation with a centrifugal force greater than a centrifugal force used in above-mentioned centrifugation is carried out on residual blood plasma, obtained sediment is collected and is subjected to freezing-thawing treatment respectively at a temperature no more than -20 DEG C and a temperature no less than normal temperature, and the sediment is removed through separation so as to obtain human platelet-rich plasma. The invention has the following beneficial effects: raw materials used inthe method are widely available; the blood resource is fully utilized; the obtained product of platelets is purer, contains more abundant nutritional components including growth factors and has a more ideal effect when used for isolation and culture of human mesenchymal stem cells.

The invention provides a mesenchymal stem cell serum-free medium. The mesenchymal stem cell serum-free medium is prepared from, by volume, 94-97 parts of DMEM / F12, 2-5 parts of human blood platelet lysate and 1 part of non-essential amino acid. The invention further provides application of the medium and isolation and cultivation methods of the mesenchymal stem cell serum-free medium. The serum-free medium can be used for effectively separating and cultivating mesenchymal stem cells derived from placentae and umbilical cords, the effects are superior to commercially available media, the cost is low, the safety is high, and the application prospect is good.

Methods for forming activated platelet gels using nsPEF's and applying the activated gels to wounds, such as heart tissue after myocardial infarction. The platelets are activated by applying at least one nsPEF with a duration between about 10 picoseconds to 1 microsecond and electrical field strengths between about 10 kV / cm and 350 kV / cm.

The invention discloses a stem cell culture medium. The stem cell culture medium comprises an improved DMEM (Dulbecco's Modified Eagle Medium) / F12 basal culture medium and an additive, wherein the additive comprises sodium bicarbonate, selenium amino acid chelate, recombinant human insulin growth factors, recombinant human basic fibroblast growth factors, recombinant human lactoferrin, ascorbic acid, recombinant human platelet-derived growth factors, recombinant human vascular endothelial cell growth factors, octacosanol, polyvinyl alcohol, polyvinylpyrrolidone and recombinant human epidermal growth factors. According to the stem cell culture medium, the potential of stem cells is not influenced while the stem cells can be proliferated rapidly, the proliferation speed of the stem cells is increased by 3-5 times compared with a common culture medium, and further, the stem cell culture medium can be used for culturing the stem cells of various kinds of tissue and has excellent applicability; the cultured stem cells have high differentiation capability, can be differentiated into multiple functional cells and have very high scientific research and medical application values, culture medium components are exact, the quality is stable, and accordingly, the cultured stem cells are not likely to generate human body rejection reaction after transplanting.

Methods for forming activated platelet gels using nsPEF's and applying the activated gels to wounds, such as heart tissue after myocardial infarction. The platelets are activated by applying at least one nsPEF with a duration between about 10 picoseconds to 1 microsecond and electrical field strengths between about 10 kV / cm and 350 kV / cm.

Invented is a method for the preservation of human platelet lifespan and / or efficacy during storage which comprises the addition of an effective amount of a non-peptide TPO receptor agonists to a storage solution containing human platelets.

The present invention provides an antibody which has the following features, its active fragment, or a derivative thereof:a) It specifically binds to human platelet membrane glycoprotein VI (GPVI);b) The function to activate a platelet and / or the function to induce a thrombocytopenia in vivo are low; andc) It at least partially depletes GPVI on the platelet membrane by contacting with a platelet.

A ligand derived from, e.g. a Fab fragment of, a monoclonal antibody obtainable from the cell line deposited with the Belgian Coordinated Collections of Micro-organisms under accession number LMBP 5108CB binds to the human platelet glycoprotein GPib and prevents the binding of von Willebrand factor to said GPIb without inducing thrombocytopenia. The said ligand is useful, in admixture with a pharmaceutically acceptable carrier, in a pharmaceutical composition, optionally further comprising a thrombolytic agent, for preventing and / or treating haemostasis disorders.

A ligand derived from, e.g., a Fab fragment of a monoclonal antibody obtainable from the cell line deposited with the Belgian Coordinated Collections of Micro-Organisms under accession number LMBP 5108CB binds to the human platelet glycoprotein GPIb and prevents the binding of von Willebrand factor to said GPIb without inducing thrombocytopenia. The said ligand is useful, in admixture with a pharmaceutically acceptable carrier, in a pharmaceutical composition, optionally further comprising a thrombolytic agent, for preventing and / or treating haemostasis disorders.

The present invention relates to polypeptides to be administered especially to humans and in particular for therapeutic use. The polypeptides are modified polypeptides whereby the modification results in a reduced propensity for the polypeptide to elicit an immune response upon administration to the human subject. The invention in particular relates to the modification of human thrombopoietin (TPO) to result in TPO proteins that are substantially non-immunogenic or less immunogenic than any non-modified counterpart when used in vivo.