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Serum-free and heterologous component-free mesenchymal stem cell culture medium and application thereof

A technology of mesenchymal stem cells and culture medium, applied in the field of mesenchymal stem cell culture medium, can solve the problems of restricting the industrial transformation and clinical application of mesenchymal stem cells, poor batch stability of culture medium, and low efficiency of adipocytes, etc. Proliferation ability, improve multi-directional differentiation function, improve the effect of immune regulation ability

Active Publication Date: 2020-07-28
BEIJING TRANSGEN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But, all there is following shortcoming in the developed serum-free mesenchymal stem cell culture medium: 1) complex composition
There are as many as ten or more types of ingredients, resulting in poor stability between batches of culture media and difficulties in quality control; 2) the immune regulation function of mesenchymal stem cells cannot be well maintained
The ability of the cultured mesenchymal stem cells to secrete the immunoregulatory factor PGE2 and inhibit the proliferation of PBMC cells is weak; 3) the multidirectional differentiation potential of mesenchymal stem cells cannot be well maintained
The directed differentiation of cultured mesenchymal stem cells into osteoblasts, chondrocytes, and adipocytes is low
These problems largely limit the industrial transformation and clinical application of mesenchymal stem cells

Method used

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  • Serum-free and heterologous component-free mesenchymal stem cell culture medium and application thereof
  • Serum-free and heterologous component-free mesenchymal stem cell culture medium and application thereof
  • Serum-free and heterologous component-free mesenchymal stem cell culture medium and application thereof

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Experimental program
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Effect test

Embodiment 1

[0109] Embodiment 1 A kind of serum-free, xenogeneous component-free mesenchymal stem cell culture medium

[0110] A serum-free, heterogeneous-free mesenchymal stem cell culture medium, said culture medium is composed of DMEM / F12 medium (1×), human platelet lysate and human lipocalin 2, said human platelet lysate The volume content of the liquid in the mesenchymal stem cell medium is 20%, and the content of the human lipocalin 2 in the mesenchymal stem cell medium is 200 μg / mL.

[0111] The pH value of the mesenchymal stem cell culture medium is 7.38, and the osmotic pressure is 341 mOsm / kg.

Embodiment 2

[0112] Embodiment 2 A kind of serum-free, xenogeneous component-free mesenchymal stem cell culture medium

[0113] A serum-free, heterogeneous-free mesenchymal stem cell culture medium, said culture medium is composed of DMEM / F12 medium (1×), human platelet lysate and human lipocalin 2, said human platelet lysate The volume content of the liquid in the mesenchymal stem cell medium is 10%, and the content of the human lipocalin 2 in the mesenchymal stem cell medium is 100 μg / mL.

[0114] The pH value of the mesenchymal stem cell culture medium is 7.35, and the osmotic pressure is 335mOsm / kg.

Embodiment 3

[0115] Embodiment 3 A kind of serum-free, xenogeneous component-free mesenchymal stem cell culture medium

[0116] A serum-free, heterogeneous-free mesenchymal stem cell culture medium, said culture medium is composed of DMEM / F12 medium (1×), human platelet lysate and human lipocalin 2, said human platelet lysate The volume content of the liquid in the mesenchymal stem cell medium is 5%, and the content of the human lipocalin 2 in the mesenchymal stem cell medium is 50 μg / mL.

[0117] The pH value of the mesenchymal stem cell culture medium is 7.42, and the osmotic pressure is 346mOsm / kg.

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Abstract

The present invention discloses a serum-free and heterologous component-free mesenchymal stem cell culture medium and an application thereof, and firstly discloses the mesenchymal stem cell culture medium. The mesenchymal stem cell culture medium comprises a basal culture medium and a supplement, the basal culture medium is a DMEM / F12 culture medium, and the supplement is a human platelet lysate and / or human lipocalin 2. The present invention further discloses the application of the mesenchymal stem cell culture medium in culturing mesenchymal stem cells. The provided mesenchymal stem cell culture medium is free of serum and heterologous components, ensures safety of clinical application, is simple in components, good in batch-to-batch stability and strong in cell expansion ability in vitro, meets industrial transformation requirements, can well maintain immune regulation ability and multi-directional differentiation ability of the mesenchymal stem cells, and ensures effective functions in clinical treatment.

Description

technical field [0001] The invention relates to the technical field of cell culture. More specifically, it relates to a serum-free, xenogeneous component-free medium for mesenchymal stem cells and its application. Background technique [0002] Mesenchymal stem cells are a type of adult stem cells with self-replication, renewal and multi-lineage differentiation potential, which widely exist in fetal and adult tissues. In addition to self-renewal, mesenchymal stem cells have unique immune regulation and tissue regeneration functions. On the one hand, mesenchymal stem cells regulate immune cells through the interaction with immune cells and paracrine soluble factors, mediate the balance of immune responses, and help the body form a microenvironment that is conducive to the repair or regeneration of damaged tissue cells. The immune regulation of mesenchymal stem cells includes inhibiting the activation of T lymphocytes, reducing the activation and proliferation of B cells and ...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0663C12N5/0665C12N5/0667C12N2500/84C12N2501/998
Inventor 佟曼付丽娜薛博涵马静辛文
Owner BEIJING TRANSGEN BIOTECH CO LTD
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