The invention discloses a novel activating agent for immune cell amplification and a preparation method thereof. The novel activating agent is prepared from the following ingredients in parts by weight: 20 to 26 percent of biological conductive nanometer fiber, 1 to 3 percent of power generation cells, 3 to 4 percent of gel mixed solution, 9 to 11 percent of composite agonists, 12 to 14 percent ofartificial cell membranes, 0.3 to 0.8 percent of cell membrane activators, 8 to 10 percent of specific antibodies and the balance of culture base solution. A great part of biological conductive nanometer fiber is used for performing loading on the composite agonists; then, the artificial cell membranes are used for coating; finally, after the treatment and activation by cell membrane activating materials, a specific antibody is combined; the power generation cells and the gel mixed solution are planted on the other small part of biological conductive nanometer fiber; then, the two parts of biological conductive nanometer fiber are put into a culture base solution to form the novel activating agent for immune cell amplification. Generally, the novel activating agent can improve the in vitro proliferation rate of immune cells by 10 to 15 times.