38results about How to "Promote directed differentiation" patented technology

The invention relates to the technical field of cell culture, and specifically provides a method for obtaining macrophages with phagocytic function through differentiation of pluripotent stem cells. The cell induction medium can realize rapid and large-scale induction of embryoid somatic cells to differentiate into macrophages. Among them, the first six media include serum-free media, which can provide basic nutrients for cell growth, proliferation and differentiation at various stages, and avoid the introduction of exogenous substances to reduce the risk of contamination. The seventh medium contains serum and additionally added FBS, which can well maintain the growth of macrophages. Each medium contains various cytokines which can promote the directional differentiation of cells. The cell induction culture method provided by the present invention is simple, efficient, low in cost, and can be expanded in a large amount in a short period to obtain high-quality and high-purity macrophages, which is of great significance for clinical treatment and research.

The invention discloses an antibacterial macromolecular polyvinyl alcohol material, a method and an application thereof. The antibacterial macromolecular polyvinyl alcohol material uses a silicon chip as a base material, and prepares a PVA-TP-CA polymer coating on the silicon chip. The polymer coating further introduces Co 2+ Ion, which has the structural formula shown in formula (1). The material of the present invention forms Co 2+ ion‑terpyridine complexes, the terpyridine group provides a platform for the efficient anchoring of bioactive cobalt ions with good control over the introduced Co 2+ The concentration of ions avoids the cytotoxic reaction and protein adsorption induced by high concentrations of metal ions, and ensures that Co 2+ The ions are stably fixed on the surface of the material to avoid being released into liquid matrices such as cell culture fluid. co 2+ The ions can provide long-term antibacterial ability on the surface of the material and improve the mechanical strength of the polymer coating surface, thereby having a physical stimulation effect of promoting the directional differentiation of bone marrow mesenchymal stem cells into bone cells.

The invention relates to the field of cell engineering, and in particular relates to a culture medium and a culture method for inducing multipotent stem cells for directional differentiation of renal cells. The culture medium for inducing the multipotent stem cells for directional differentiation of the renal cellscomprises 10-15g / L of a DEME basal medium, 0.1-0.5mM of mannitol, 4-8U of heparin sodium, 1-1.5mM of L-glutamine, 0.5-0.85mM of sodium pyruvate, 0.15-0.2mM of beta-mercaptoethanol, 1-3mg / L of fibronectin, 27-39ng / mL of directional differentiation induction factors, and a serum replacement. The culture medium for inducing the multipotent stem cells for directional differentiation of the renal cells, provided by the invention, can effectively promote induction of the directional differentiation from the multipotent stem cells to the renal cells, and no fetal calf serum is added in the culture medium, thereby avoiding a risk of infection of animal pathogens.

The invention relates to the field of biotechnology and in particular relates to a PF-127-LV-NFcocktail compound loaded with portable neural stem cells as well as a preparation method and application of the PF-127-LV-NFcocktail compound. The compound comprises Pluronic F-127 hydrogel, a complete neural stem cell culture medium, a Lingo-1 shRNA slow virus expression vector and a nerve growth factor mixture. The preparation method of the PF-127-LV-NFcocktail compound comprises the following steps: 1, dissolving the Pluronic F-127 hydrogel in the complete neural stem cell culture medium; 2, adding the Lingo-1 shRNA slow virus expression vector; 3, adding the nerve growth factor mixture; and 4, performing filtration and sterilization. The compound can load the portable neural stem cells and can promote directional differentiation and nerve regeneration of the neural stem cells.

The invention discloses a preparation method of a tissue engineering scaffold with bioactivity. The preparation method comprises the following steps: crosslinking thermally treated soybean proteins by using oxidized sodium alginate under the condition of high speed stirring; and freezing and drying to obtain the soybean protein-based tissue engineering scaffold with bioactivity. The porous bracket prepared by the method has a relatively great bore diameter and porosity and good biocompatibility, has certain bioactivity, can be used for promoting secretion of cellular matrixes and directional differentiation of stem cells and can be used as an ideal carrier of the cell in the tissue engineering field.

The invention discloses the preparation and application of flavonol as a brain targeting synergist. The inventors have found in long-term experimental research that some flavonol compounds, especially kaempferol, rutin, troxerutin, myricetin, hesperidin, and their hydroxy derivatives, especially their glycosides, esters, ethers Derivatives, can be very good to promote the therapeutic drug molecules ginsenoside, stilbene glycoside, resveratrol, levodopa, edaravone, vinpocetine, nicergoline, cytosine When choline, oxiracetam, etc. enter the brain tissue, the concentration of the drug in the brain tissue is greatly increased without increasing its blood concentration, and the curative effect of the drug is effectively improved.

The invention discloses the application of a piezoelectric material in the proliferation and / or differentiation of stem cells, wherein ultrasonic waves are used to excite the piezoelectric effect of the piezoelectric material. When the piezoelectric material is applied to the proliferation and / or differentiation of stem cells, it will not reduce the viability and stemness of the stem cells, but can also maintain good biocompatibility and promote the proliferation of stem cells; and, by using ultrasound to excite the piezoelectric material The piezoelectric effect can convert the mechanical signal of ultrasound into an electrical signal and provide it to stem cells, which is beneficial to induce the directional differentiation of stem cells.