56 results about "Disease phenotype" patented technology

A computer-assisted method of looking for pharmacologic targets, in which large numbers of persons are enrolled in drugh clinical trials, they are medically examined and documented, tissue samples are taken, the tissue samples are genotyped, and an examination is made of the genotypes to try to ascertain associations between the genotypes and the documented disease phenotypes of the patients.

Physiological imaging-defined subvolumes of tissues/disease are identified to yield spatially-defined prognostic and/or predictive indicators and/or focal therapy targets within such tissues, in particular tumors, for evaluation over time, for example, prior to and after a therapy treatment. Medical image data is analyzed to delineate subvolumes of tissue based upon multiple physiological, metabolic, and biological imaging properties, where those subvolumes are extracted and analyzed in a probabilistic manner to associate with one or more abnormal or disease phenotype conditions.

The invention belongs to the field of researching and application of biomedicine and particularly relates to human pluripotent stem cell-hereditary cardiomyopathy cardiac muscle cells and a preparation method thereof. The invention provides a human hereditary cardiomyopathy-pluripotent stem cell, which is constructed by means of TALEN or CRISPR/CAS9 genome editing technology. The human hereditary cardiomyopathy cardiac muscle cell can be combined with any scaffold materials to culture various in-vitro human hereditary cardiomyopathy cardiac muscle tissues. The human hereditary cardiomyopathy cardiac muscle cells in the invention have disease phenotypes and electrophysiology change being similar as the cardiac muscle cells of human hereditary cardiomyopathy patients. The human hereditary cardiomyopathy cardiac muscle cells are wide in sources and can be cultured in-vitro for a long time. The invention provides excellent tools and platforms for researching an effective new therapy means and researching an effective corresponding treatment medicine.

The present invention discloses disease-linked SNPs, microRNAs, and microRNA-targeted mRNAs relevant to the pathogenesis of several major human disorders including, but not limited to, multiple types of cancers, type 2 diabetes, type 1 diabetes, Crohn's disease, coronary artery disease, hypertension, rheumatoid arthritis, bipolar disorder. Also provided are methods for the identification of disease phenotype-defining sets of SNPs, microRNAs, and mRNAs that are defined here as a “consensus disease phenocode” as well as methods of using the information provided by these consensus disease phenocodes for various diagnostic, prognostic, and/or therapeutic applications.

The present invention is directed to methods and products for defining a biomarker of disease phenotype. The present invention further relates to methods and kits for determining a subject's risk of developing recurrent hepatocellular carcinoma based on a defined microRNA biomarker that reliably distinguishes hepatocellular carcinoma disease recurrence from non-recurrence. The invention also relates to methods of treating a patient having heptocellular carcinoma based on their risk of developing hepatocellular carcinoma disease recurrence.

The invention belongs to the field of biomedicine, and relates to a safe and reliable reprogrammed method for obtaining human iPS (induced pluripotent stem) cells, a method for simply, rapidly and efficiently inducing and differentiating iPS cells into spinal motoneurons, and a method for economically and efficiently obtaining functional spinal motoneurons and application of the method. Human fibroblasts are infected with Sendai virus carrying a reprogramming factor by using vitronectin as the sole growth medium, and are cultured for 3 weeks to obtain human iPS cells without exogenous serum interference; the iPS cells are efficiently induced and differentiated into spinal motoneurons by a combination of multiple small molecule compounds; and further, an astrocyte conditioned culture medium promotes maturation culture and is applied in the formation of neuromuscular junctions. The functional spinal motoneurons obtained by efficient induction can be applied to simulation of disease phenotype and discussion of pathogenesis, and provides a theoretical basis for the screening of effective therapeutic drugs in the future, and even in the future can be applied to the cell transplantation therapy of diseases.

A current system allows data-driven hypothesis generation to identify therapeutic candidates for a disease phenotype treatment by identifying drugs and clinical indications associated with lower occurrences of disease-associated phenotype(s) by a drug/drug class. A current system may include a pharmaceutical hierarchical ontology; a phenotype hierarchical ontology; a record database comprising clinical event records; a database mining engine; and a mapping engine. The database mining engine may iteratively progress through a portion of the pharmacological hierarchical ontology and phenotype hierarchical ontology to iteratively select pairs of cohort entries from each ontology; and for each pair of cohort entries, query the clinical record database for matching records. The mapping engine may map each pair of cohort entries into a matrix comprising a drug-event cell for each pair and apply a value thereto representing the number of database records matching items returned by the database mining engine for the corresponding cohort entries.

The invention discloses a method for screening bio-control bacteria of cucumber fusarium wilt, and provides a method for identifying the bio-control nature of to-be-identified bacteria to pathogenic bacteria of the cucumber fusarium wilt including experimental treatment. The experiment treatment sequentially includes following steps of 1), accelerating sprouting of sterilized cucumber seeds to obtain the sprouting seeds; 2), subjecting the sprouting seeds to soaking in suspension liquid of the to-be-identified bacteria prior to cultivating the sprouting seeds till radicle length reaches by 1.5-2.0cm; 3), inoculating cucumber seedlings to suspension liquid of the pathogenic bateria of the cucumber fusarium wilt; 4), transferring the cucumber seedlings to MS solid media to cultivate, and identifying the bio-control nature of the to-be-identified bacteria to the patheogenic bacteria of the cucumber fusarium wilt by surveying disease indexes and/or observing disease phenotypes. The method is applicable to screening the bio-control bacteria of the cucumber fusarium wilt. In the method, the cucumber seedlings used as samples can truly reflect disease prevention effect of the bio-control bacteria, and can screen bacterial strains of various bio-control mechanisms.

The invention relates to an application of an Arabidopis thaliana transcription factor at3g46080 gene. By constructing a CDS sequence of the Arabidopsis thaliana at3g46080 gene into the upstream of aFLAG protein label by a Gateway technology, Arabidopsis thaliana is conversed, which affects the disease phenotype of Arabidopsis thaliana, screening and identification are carried out, and finally the transgenic plants can be obtained. The analysis of the screened plants carried out so that the plant has enhanced resistance to Pseudomonas lycoposum by Arabidopis thaliana, the gene has the same function for homologous genes in crops, and thus has important significance in production.

The embodiment of the invention provides a model training method, a mechanical ventilation phenotype recognition method and a respirator. The model training method comprises the steps that multi-dimensional feature data of a testee are acquired, and the multi-dimensional feature data at least comprise basic and scoring features, breathing features, circulation and perfusion features and residual feature in-out quantity balance of the testee; complementation and correlation analysis are performed on the multi-dimensional feature data, so that analysis data can be obtained; and Gaussian mixtureclustering is carried out based on the analysis data, model parameters are learned, and a Gaussian mixture model is determined according to the model parameters. According to the embodiment of the invention, the phenotype of the corresponding disease is determined by collecting the characteristic parameters related to the certain disease treatment equipment, and subsequent doctors can perform classified treatment and nursing on different patients according to the combination of the phenotype type of the disease and the related observation data of the certain disease treatment equipment of thepatients.

The invention discloses a gene module analysis method. The gene module analysis method comprises the following steps: inputting a gene phenotype double-layer network, a gene function similarity network and a known disease gene set s0 related to a disease phenotype; increasing the connection relationship between genes and phenotypes in the gene phenotype double-layer network; taking genes which arein edge connection with disease genes in s0 and are not in s0 in the gene phenotypic double-layer network as candidate genes; calculating and selecting a candidate gene with the maximum sum of semantic similarity, topological similarity and phenotypic relevance, and adding the candidate gene into s0; and when the expanded candidate gene set does not significantly enrich the GO ontology function annotation and the biological pathway gene related to the disease phenotype at the same time and express the differential gene in the disease phenotype sample and the normal sample any more, recordingthe current algebra as m, and outputting m-1 as candidate genes expanded in the first generation s0 and known disease genes connected with the edges of the expanded candidate genes.

Methods and compositions for identifying an agent (e.g., a gene product or small molecule compound) that modulates a Parkinson's disease phenotype are provided. In practicing the subject methods, a non-mammalian animal model, such as Drosophila melanogaster, that includes a mutant parkin gene and at least one other mutant gene are evaluated for a Parkinson's disease phenotype. Also provided are kits, and systems for practicing the subject methods, as well as methods of use of agents identified in the screening method of the invention.

High throughput sequencing has facilitated a precipitous drop in the cost of whole genome human DNA sequencing, prompting predictions of a revolution in medicine via personalization of diagnostic and therapeutic strategies to individual genetics. Disclosed is a comprehensive series of methods for identification of genetic variants and medical genotypes, phasing genetic data and using Mendelian inheritance for quality control, and providing predictive genetic information about risk for rare disease phenotypes and response to pharmacological therapy in single individuals and father-mother-child trios.

The invention discloses a drug-target-disease interaction prediction method based on a hypergraph. The method comprises the following steps: 1) establishing a semantic hypergraph G according to a binary relationship R between every two drugs, targets and diseases; according to the drug molecule fingerprints, the target sequences and the disease phenotypes, establishing feature similarity graphs of drug nodes, target nodes and disease nodes; 2) applying a hypergraph neural network on the semantic hypergraph G to obtain node representations corresponding to drugs, targets and diseases; respectively applying a graph convolutional network on the feature similarity graphs of the drugs, the targets and the diseases to obtain node representations corresponding to the drugs, the targets and the diseases; 3) fusing the node representations obtained in the step 2); 4) training a prediction model by using the fused node representation corresponding to each hyperedge obtained in the step 3); 5) generating node representations of a to-be-predicted drug a and a to-be-predicted disease c, and inputting the node representations into the trained prediction model for prediction to obtain a prediction probability that the drug a treats the disease c through the target b.

The invention relates to the technical field of bioinformatics, in particular to a method for screening disease phenotype related mutation sites and application thereof. The method comprises the following steps: obtaining sequencing data of a plurality of disease samples and normal samples, and carrying out variation detection; performing association rule mining by taking the phenotype of the samples and the mutation type of the detected mutation site as a total project set to obtain a mutation site having a strong association relationship with the phenotype of the disease samples; and performing modeling analysis on the mutation sites obtained through association rule mining and screening to obtain mutation sites related to disease phenotypes. Alleles are converted into classification variables for association rule mining, and then modeling analysis is carried out on sites strongly associated with disease phenotypes, so that the total quantity of analyzed samples can be effectively reduced, and the influence of allele frequency on an analysis result is avoided; and screening and analysis of disease phenotype related sites can be completed only by obtaining mutation genotype information.

The invention discloses a semantic structuralization processing method for the medical text phenotypic information. A finer and more accurate disease phenotypic knowledge database is obtained by constructing a phenotypic semantic structure unit, automatically identifying the phenotypic semantic structure unit and interactively proofreading the phenotypic semantic structure unit. The method is advantaged in that phenotypic knowledge in the medical text can be subjected to more accurate and deeper structured representation on the semantic level, and fine-grained representation on the semantic level is deeper. According to the method, development of the medical informatization and intelligent career of China can be greatly promoted.

The invention discloses a queue identification method and device applied to a special disease clinical test. The method comprises the following steps: based on a pre-established knowledge system of aspecial disease clinical test in-and-out standard, generating query information of the special disease clinical test in-and-out standard; acquiring electronic medical record data, and performing information analysis on the electronic medical record data to obtain special disease phenotype analysis information; and based on the special disease phenotypic analysis information and the query information of the special disease clinical test in-and-out standard, determining a special disease clinical test queue matched with the query information. According to the invention, the knowledge system of the special disease clinical test in-and-out standard is a system for expressing the special disease clinical test in-and-out standard by knowledge of computer recognizable information; the in-and-outstandard is automatically converted into the structured query language, the electronic medical record data can be directly processed, a subject meeting the special disease clinical test is obtained, the information conversion processing efficiency is improved, and the accuracy of the queue identification work of the clinical test is improved.

The invention discloses a cotton verticillium wilt disease phenotype recognition method. The method comprises the following steps of 1, cultivating cotton seedlings in a non-woven fabric seedling raising bag; step 2, acquiring a canopy spectrum curve by utilizing a near-infrared multispectral technology; step 3, analyzing sensitive waveband correlation among different resistant varieties; step 4, establishing an estimation model according to the waveband combination; and step 5, obtaining a sensitive waveband corresponding to the disease grade. The method disclosed by the invention is low in destructiveness, simple to operate, labor-saving, high in inoculation success rate, uniform and consistent in morbidity and suitable for carrying out large-batch material identification work on vast scientific research workers, the bottoms of the nutrition pots do not need to be cut off, and the accuracy is higher than that in the prior art.

The invention discloses a non-therapeutic-purpose gene editing method based on PINK1 and PARK7 through injection of a virus into specific brain regions of animals. The virus is directly injected intothe specific brain regions of non-human primates by using a brain stereotaxic injection pump, wherein the injection volume is 1 [mu]L of the injected virus per 1 m<3>, and the titer of the injected virus is 10*10<13>vg/mL. Through the method, gene editing is performed directly on the specific brain regions of the non-human primates, and transgenic monkey models with disease phenotypes can be obtained in a short time, so that urgent research needs of Parkinson's syndromes (PD) at the present stage are met.