709 results about "Biological imaging" patented technology

The invention belongs to the technical field of preparation of a carbon nano material, and particularly relates to a method for preparing a carbon dot having a high fluorescent quantum yield from citric acid and different nitrogen-containing molecules. The method comprises the following steps: weighing 1-10 mmol of solid citric acid, and dissolving in 10 ml of deionized water; weighing 1-10 mmol of ethylenediamine, ethylamine, propylamine, butanediamine, n-hexylamine, p-phenylene diamine or urea, adding into the citric acid solution, and uniformly stirring; transferring the solution into a hydrothermal or high-pressure microwave reaction kettle, reacting under hydrothermal or microwave conditions, and naturally cooling the reaction kettle to room temperature, thus obtaining a yellow or brown carbon dot water solution; and finally, purifying the carbon dot water solution, evaporating, and drying to obtain pure carbon dot solid powder. The carbon dot solution can send out bright blue fluorescence under the irradiation of a handheld ultraviolet lamp. The invention has wide application prospects in the fields of biological imaging, fluorescent printing and the like.

Fused ring bridge, ring locked dyes that form thermally stable photorfractive compositions. The fused ring bridge structures are pi -conjugated bonds in benzene-, naphthalene- or anthracene-derived fused ring systems that connect donor and acceptor groups. The donor and acceptor groups contribute to a high molecular dipole moment and linear polarizability anisotropy. The polarization characteristics of the dye molecules are stabilized since the bonds in the fused ring bridge are not susceptible to rotation, reducing the opportunity for photoisomerization. The dyes are compatible with polymeric compositions, including thermoplastics. The dyes are electrically neutral but have charge transport, electronic and orientational properties such that upon illumination of a composition containing the dye, the dye facilitates refractive index modulation and a photorefractive effect that can be utilized advantageously in numerous applications such as in optical quality devices and biological imaging.

A system and method for managing imaging data related to a biological trait, including generating a three-dimensional representation of the external surface of the body a patient; obtaining the imaging data; associating the imaging data with a coordinate location of the three-dimensional representation, to generate an associated imaging data; and managing the associated imaging data using the surface representation. The association includes uniquely associating numerical values (e.g., Cartesian coordinates) to particular surface features. The three-dimensional model includes the ability to adjust the pose of the 3D models made from data taken at different times so that the coordinate systems closely correspond.

The invention discloses a carbon nano-dot, and a preparation method and an application thereof, and solves a problem that the application of present nano-dots is restricted because of high preparation cost and easy fluorescent quenching appearance of an aggregate state. According to the invention, the carbon nano-dot having a high fluorescence quantum efficiency is prepared through adopting a polycarboxyl or polyhydroxy contained organic compound, or an amino acid as a raw material, and urea as a surface passivation modification agent, and through a microwave process, and a carbon nano-dot fluorescent ink is prepared through using the carbon nano-dot. The preparation method disclosed in the invention has the advantages of simplicity, low cost and convenient large-scale production; the fluorescent quenching of the prepared carbon nano-dot on the surface of a biological product does not appear, and the highest fluorescence quantum efficiency is 42%; and the prepared carbon nano-dot fluorescent ink is nontoxic, does not generate a precipitate after long-time dispose, and can be applied to the biological imaging field, the biological product identification field, the information storage field, the information encryption field, the false proof field, the illumination display field, the photovoltaic device field and the like.

Described herein are catheters for use with Optical Coherence Tomography (OCT) that include an optical fiber core having a first refractive index and an interface medium having a second refractive index, where the first and second refractive indexes are mismatched such that receiving electronics configured to receive optical radiation reflected from the reference interface and the target operate in a total noise range that is within 5 dB of the shot noise limit. These OCT catheters may include a silicon die mirror having a reflective coating that is embedded in the interface medium. The optical fiber can be fixed at just the distal end of the catheter, and may be managed within a handle that is attached to the proximal end of the catheter body, and is configured to allow rotation of the both catheter body and the optical fiber relative to the handle.

The invention belongs to the technical field of the preparation of graphene quantum dots (GQDs), and particularly relates to a method for preparing fluorescent graphene quantum dots with controllable oxidation degree and fluorescence by a solvothermal method. According to the technical scheme, the method comprises the following steps of: 1, preparing graphene oxide; 2, preparing green fluorescent graphene quantum dots by a single-step method starting from the graphene oxide; and 3, preparing the fluorescent graphene quantum dots with the controllable oxidation degree by a column chromatographic separation method. According to the method, the sizes and surface oxidation degree of the graphene quantum dots can be controlled under the synthetic condition, so that the fluorescent properties and surface chemical characteristics of the graphene quantum dots are controlled. The prepared graphene quantum dots are high in chemical stability and biocompatibility, low in biotoxicity, and high in property of applicable upconversion fluorescence, matt and the like and bleaching performance. By the excellent properties, the graphene quantum dots have a wide application range in aspects of biological imaging, photovoltaic devices and sensors, and are novel promising fluorescent nano materials.

The invention discloses a nitrogen doped carbon quantum dot as well as a preparation method and an application thereof, belonging to the technical field of material science. The preparation method comprises the steps: after mixing organic acid and organic amine, directly performing a hydrothermal reaction to prepare a nitrogen doped carbon quantum dot water dispersion solution, and extracting and drying the nitrogen doped carbon quantum dot water dispersion solution to obtain a nitrogen doped carbon quantum dot solid. The size of the nitrogen doped carbon quantum dot is not more than 10 nm, the nitrogen content is not more than 50 percent, the surface of the nitrogen doped carbon quantum dot contains amino, carboxyl and hydroxyl; and the quantum efficiency is 39.8-50 percent. The preparation method is simple to operate, environment-friendly, low in equipment requirement; and the prepared nitrogen doped carbon quantum dot has excellent light emitting property, and can be widely applied to fields such as photoelectric materials, biological imaging and fluorescence probe.

The invention relates to a preparation method of a nano-particle@minisize metal organic frame material. The preparation method comprises the following steps of 1, preparing nano-particles modified by polyvinylpyrrolidone; 2, adding the obtained nano-particles into an MOF growth reaction solution, the addition amount of an adjusting agent is controlled, and the nano-particle@minisize metal organic frame material is obtained. The method is simple and easy to implement, mild in condition and high in applicability, and the prepared nano-particle@minisize metal organic frame material can effectively prevent nano-particle aggregation, meanwhile has the advantages of being good in size selectivity, and short in diffusion range between the surface of the metal organic framework and the active center of nano-particles, and has the wide application prospect in the fields of catalysis, biological imaging and the like.

Physiological imaging-defined subvolumes of tissues / disease are identified to yield spatially-defined prognostic and / or predictive indicators and / or focal therapy targets within such tissues, in particular tumors, for evaluation over time, for example, prior to and after a therapy treatment. Medical image data is analyzed to delineate subvolumes of tissue based upon multiple physiological, metabolic, and biological imaging properties, where those subvolumes are extracted and analyzed in a probabilistic manner to associate with one or more abnormal or disease phenotype conditions.

An embodiment of the invention is method, which can be implemented in software, firmware, hardware, etc., for virtual fly over inspection of complex anatomical tubular structures. In a preferred embodiment, the method is implemented in software, and the software reconstructs the tubular anatomical structure from a binary imaging data that is originally acquired from computer aided tomography scan or comparable biological imaging system. The software of the invention splits the entire tubular anatomy into exactly two halves. The software assigns a virtual camera to each half to perform fly-over navigation. Through controlling the elevation of the virtual camera, there is no restriction on its field of view (FOV) angle, which can be greater than 90 degrees, for example. The camera viewing volume is perpendicular to each half of the tubular anatomical structure, so potential structures of interest, e.g., polyps hidden behind haustral folds in a colon are easily found. The orientation of the splitting surface is controllable, the navigation can be repeated at another or a plurality of another split orientations. This avoids the possibility that a structure of interest, e.g., a polyp that is divided between the two halves of the anatomical structure in a first fly over is missed during a virtual inspection. Preferred embodiment software conducts virtual colonoscopy fly over. Experimental virtual fly over colonoscopy software of the invention that performed virtual fly over on 15 clinical datasets demonstrated average surface visibility coverage is 99.59+ / −0.2%.

The present invention discloses a graphene quantum dot preparation method, belongs to the technical field of nanometer material preparation, and particularly relates to a graphene quantum dot preparation method. The technical scheme comprises that: a carbon material being subjected to deep oxidation is cut into graphene oxide nano-sheets, C=C bonds and C-C bonds of the graphene oxide are broken to prepare green fluorescence graphene oxide quantum dots, and a reduction or high temperature annealing method is further adopted to prepare the blue fluorescence graphene quantum dots. According to the present invention, the operations are simple, the raw materials are easy to obtain, and the prepared graphene quantum dots have characteristics of excellent monodispersity, good water solubility and strong fluorescence, and have potential application prospects in the fields of supercapacitors, lithium batteries, biological imaging, solar cells, field-effect transistors, OLEDs and the like.

The invention relates to a surface-enhanced Raman scattering probe and a preparation method thereof. The probe is an interlayer structure and consists of a core precious-metal nano-rod, an intermediate sandwich layer and a precious-metal shell layer which grows and is formed on the outer surface, wherein a Raman signal molecule is wrapped inside the intermediate sandwich layer. The preparation method comprises the following steps that: the surface of the precious-metal nano-rod absorbs or is coupled with the Raman signal molecule, then the Raman signal molecule is wrapped to form the intermediate sandwich layer through silicon dioxide or polyelectrolyte, and then the precious-metal shell layer grows and is formed outside the intermediate sandwich layer. Through the surface-enhanced Raman scattering probe with the sandwich structure, strong local electromagnetic density is produced through the interaction of the surface plasma between the gold nano-rod and the outer metal shell layer, so the Raman signal is greatly enhanced, the weaknesses that a two-dimensional underlay is difficult to repeat, is expensive and is complicated can be overcome, and at the same time the size of the surface-enhanced Raman scattering probe is smaller than 200nm, biological detection of living bodies and application of biological imaging can be facilitated when the surface-enhanced Raman scattering probe is used as a biological probe.

The invention discloses a nitrogen-doped fluorescent carbon quantum dot and a preparation method and application thereof, and belongs to the technical field of material science. Fish wastes (such as fish scales, bones and skins) are cleaned, then hydrothermal reaction is directly performed to obtain nitrogen-doped fluorescent carbon quantum dot aqueous dispersion, and finally filtration, dialysis and freeze-drying are performed to obtain nitrogen-doped fluorescent carbon quantum dot solid. The size of the nitrogen-doped fluorescent carbon quantum dot prepared by using the preparation method disclosed by the invention is smaller than or equal to 10nm, the nitrogen content is smaller than or equal to 20 percent, the surface of the quantum dot has nitrogen-containing and oxygen-containing functional groups, and the quantum efficiency is 17-30 percent. The nitrogen-doped fluorescent carbon quantum dot and the preparation method and application thereof have the advantages that the preparation method is environmental-friendly, the operation is simple, the requirements on equipment are low, the preparation method is suitable for large-scale production, and the prepared nitrogen-doped fluorescent carbon quantum dot has excellent fluorescent performance and can be widely applied to fields such as photoelectric materials, biological imaging and fluorescent probes.

The invention relates to a method for preparing biomass based carbon quantum dots. The method comprises the following steps: carbonizing or activating a biomass material so as to obtain a carbonized product of which the carbon content is greater than or equal to 80%, performing nano quantization on the carbonized product by using a thick acid mixing liquid so as to obtain water-soluble fluorescent carbon quantum dots, changing the reaction temperature so as to adjust the fluorescent color of the carbon quantum dots, and performing retaining separation by using a dialysis bag, thereby obtaining carbon quantum dots in a rectangular diamond cluster about 5nm. Compared with the prior art, the method is wide in raw material source based on natural biomass sources and green and environment-friendly. The chemical oxidation preparation process is simple, and preparation of small-size carbon quantum dots can be easily achieved. The carbon quantum dots are stable in fluorescent effect and good in water solubility, and can be used in fields such as biological imaging.

The invention relates to a method for preparing luminescent carbon dots from carbohydrate. The carbohydrate based carbon dots prepared by the invention can be used for biological imaging and analysis to determine the content of metal ions. The method for preparing luminescent carbon dots from carbohydrate provided by the invention is as below: mixing carbohydrates with a modifier with amino at different molar ratios; reacting for a certain time by a hydrothermal method; filtering; regulating the pH to a neutral state, conducting dialysis and purification to remove excessive salt, so as to obtain the carbohydrate based luminescent carbon dots. The method for preparing the luminescent carbon dots provided by the invention uses carbohydrates as raw materials, which are easily available and have low cost; the obtained carbon dots have high quantum yield; and the equipment for the preparation process is simple,the method can be completed in general chemistry laboratory, and is easy for popularization.

This invention provides nucleoside polyphosphate analogs each of which comprises a tag comprising a plurality of Raman-scattering moieties; compounds comprising said nucleoside polyphosphate analogs; and methods for determining the sequence of a single-stranded DNA or RNA using said nucleoside polyphosphate analogs. This invention also provides methods for detecting the interaction of a plurality of predetermined compounds, at least one of which having attached thereto a tag comprising a plurality of Raman-scattering moieties.

The invention relates to the field of a carbon nano material, and provides a nitrogen-doped fluorescent carbon-dot and a carbon-dot graphene composite as well as a production method and an application thereof. The nitrogen-doped fluorescent carbon dot is characterized in that the nitrogen element exists in the form of pyridine nitrogen, pyrrole nitrogen and graphitic nitrogen; the nitrogen atom and carbon atom are in a molar ratio of 0.035 to 0.057; the average granularity is 2.5nm to 4.1nm. The invention also provides a preparation method of a fluorescent carbon dot. The preparation method comprises the following steps: (1) carbonizing coal with high content of nitrogen to obtain a carbonized material, wherein the coal with high nitrogen content refers to the coal with the nitrogen content of 1.1 to 3.0 percent; (2) producing an anode through the obtained carbonized material, and electrochemically oxidizing the anode to obtain the fluorescent carbon dot. The invention also provides a carbon-dot-graphene composite material prepared through the nitrogen-doped fluorescent carbon dot. In addition, the invention also provides an application of the fluorescent carbon dot in the fields such as photoelectric devices, biological imaging, oxidation-reduction electric catalyst, detection and sensing and an application of an oxidation-reduction electrochemical catalyst in the field of a fuel battery.

A system for optical coherence tomography includes a source of optical radiation, an optical fiber, and a graded index fiber attached to a distal end of the optical fiber. The optical fiber and the graded index fiber are together configured to provide a common path for optical radiation reflected from a reference interface at a distal end of the graded index fiber and from a target.

A system for optical coherence tomography includes a source of optical radiation, an optical fiber, and a graded index fiber attached to a distal end of the optical fiber. The optical fiber and the graded index fiber are together configured to provide a common path for optical radiation reflected from a reference interface at a distal end of the graded index fiber and from a target.

The invention discloses a preparation method of a double-emission carbon-based nano probe prepared based on an electrostatic interaction induced self-assembling method and application of a product ofthe preparation method to in-vivo and in-vitro temperature detection of organisms, and belongs to the technical field of colorimetric fluorescence detection. The preparation method comprises synthesisof blue fluorescence carbon dots (B-CDs) which have no temperature sensitivity and negative charges on surfaces, preparation of red fluorescence carbon dots (R-CDs) which have temperature sensitivityand positive charges on surfaces and preparation of the double-emission carbon-based nano probe. According to the double-emission carbon-based nano probe obtained by the preparation method, the double-emission carbon-based nano probe has different responsiveness aiming at different temperature and has different compound temperature color changes; the double-fluorescence emission nano probe can beused for colorimetrically detecting in-vivo and in-vitro temperature of the organisms by naked eyes, and has high sensitivity (0.93 percent / DEG C) and operation repeatability, so that the double-fluorescence emission nano probe provides convenience for biological imaging, temperature sensing, environment monitoring, food safety and the like.

The invention relates to yellow fluorescence carbon dots with high quantum yield. A preparation method of the yellow fluorescence carbon dots is characterized by comprising the following steps: dissolving organic acid and organic amine in water; performing microwave radiation after fully mixing the organic acid and the organic amine, thus obtaining a brown solid crude product; performing reverse-phase silica gel column chromatography purification by taking a mixed solution of the water and methyl alcohol as an eluting agent, thus obtaining the yellow fluorescence carbon dots. The yellow fluorescence carbon dots are characterized in that the average grain diameter is 3.0 to 4.0 nm; bright yellow fluorescent light can be emitted under the irradiation of an ultraviolet lamp, the excitation wavelength is 400 to 420 nm, the emission wavelength is 510 to 550 nm, the luminescent property of solids is shown, and the quantum yield can reach 44 percent. The yellow fluorescence carbon dots prepared by the invention are good in water solubility and stable in fluorescence signals, are suitable for cell imaging under physiological conditions, have a function of nuclear staining and can be widely applied to the fields of analysis and detection, biochemical sensing, biological imaging, biological marking and the like.

Described herein are catheters for use with Optical Coherence Tomography (OCT) that include an optical fiber core having a first refractive index and an interface medium having a second refractive index, where the first and second refractive indexes are mismatched such that receiving electronics configured to receive optical radiation reflected from the reference interface and the target operate in a total noise range that is within 5dB of the shot noise limit. These OCT catheters may include a silicon die mirror having a reflective coating that is embedded in the interface medium. The optical fiber can be fixed at just the distal end of the catheter, and may be managed within a handle that is attached to the proximal end of the catheter body, and is configured to allow rotation of both the catheter body and the optical fiber relative to the handle.

The invention discloses fluorescent carbon quantum dots as well as a preparation method and an application thereof. The preparation method of the carbon quantum dots comprises the steps of adding secondary water into malic acid (or malic acid and ethanol amine, or malic acid and ethane sulfonic acid), which serves as a raw material, carrying out ultrasonic treatment so as to obtain a clarified solution, putting the clarified solution into a microwave oven, reacting for 5-20 minutes so as to obtain solid, dissolving, filtrating, and dialyzing, so as to obtain undoped (or nitrogen-doped, or sulfur-doped) water-soluble fluorescent carbon quantum dots. The preparation process of the carbon quantum dots disclosed by the invention is simple, and the prepared carbon quantum dots are stable in optical property and good in biocompatibility. The carbon quantum dots disclosed by the invention can be applied to the fields of the analysis and detection on Fe<3+> in water, the biological imaging of living cells, fluorescent ink and the like.

The invention belongs to the field of nano material preparation and provides a method to achieve surface-common-enhanced fluorescence and surface-enhanced Raman in a core-shell structure. Spherical silver nano particles are used as substrates of surface-common-enhanced fluorescence and surface-enhanced Raman, the silver nano particles are placed inside the shell layers, with good surface-enhanced Raman activity, of gold nano particles, fluorescent dye molecules are disposed between the silver nano particles and the gold nano particles, and the distances among the silver nano particles, the fluorescent dye molecules and the gold nano particles to achieve surface-common-enhanced fluorescence and surface-enhanced Raman in the core-shell structure. A Stober method is used to change corresponding experiment parameters, silicon dioxide of different thicknesses is wrapped on the surfaces of the silver nano particles to adjusting the distances among the silver nano particles, the fluorescent dye molecules and the gold nano particles, surface-common-enhanced fluorescence and surface-enhanced Raman signals of the manufactured multi-layer core-shell structure composite particles are further enhanced, and the multi-layer core-shell structure composite particles are good in water solubility, uniform and stable in nature, and promising in potential application prospect in fields such as biological imaging, safety detection and catalyzing. The method is wide in raw material source, simple and easy to operate, easy in batch and large-scale production, good in industrial production foundation, and promising in application prospect.

The invention relates to a silicon-based rhodamine-nitrogen oxide fluorescent probe. The structures of the probe are shown in the following figures (please see the figures in the specification), wherein the Lyso-SiRB-NO in the figure II has a lysosome positioning function. The probe has the advantages that excitation and emission wavelengths of the probe are located in a near infrared light area, and when the probe is applied to biological imaging, the interference of the fluorescent background of a living body itself can be reduced; the probe is high in penetrating power and little damages tissue or cells of the living body; the probe can specifically detect nitrogen oxide molecules in lysosome, and a reliable research tool is provided for research on the physiological effect of nitrogen oxide molecules.

The invention relates to near infrared fluoro-boron dipyrrole fluorescent dyes and a synthesis method of the fluorescent dyes. The dyes have the general formula shown in the specification. The fluorescent dyes are synthesized from aldehyde or imine compounds of halogenated pyrrole or isoindazole and pyrrole derivatives by a one-pot method. The emission wavelength of the fluorescent dyes is more than 600nm, and the emission spectra of the monostyryl-substituted dyes and derivatives thereof can be up to 714nm. The fluorescent dyes have excellent light, physical and chemical performances of higher fluorescent quantum yield (0.61-0.91) and better light stability and the like, and has excellent application prospects in fluorescence labeling, biological imaging and other bioanalysis fields.

The invention relates to a rare earth doped lithium yttrium fluoride nanometer material and a preparation method and application thereof. A modified high-temperature coprecipitation method is adopted to synthesize the rare earth doped lithium yttrium fluoride nanometer material with upper conversion / lower transfer luminescence, at the same time, epitaxial growth is conducted on the basis of a core, a mono-layer core-shell structure nanometer material which is uniformly covered can be obtained, and a lithium yttrium fluoride based multi-layer core-shell nanometer material is further prepared. The three prepared and obtained materials are good in monodispersity, uniform in particle size and excellent in luminescent property, after acid pickling treatment, water-soluble nanometer particles can be used for biological detection and biological imaging.

The present invention relates to fluorescent, radio-opaque and magnetic quantum nanoparticles, useful as multifunctional contrast agents or probes for in vivo bioimaging, and methods of their use. The invention provides for multifaceted bioimaging (e.g., intra-arterial pre-operative brain mapping and broad based in vivo diagnostic imaging), including imaging of various cell types, such as stem cells.

A system and method for managing imaging data related to a biological trait, including generating a three-dimensional representation of the external surface of the body a patient; obtaining the imaging data; associating the imaging data with a coordinate location of the three-dimensional representation, to generate an associated imaging data; and managing the associated imaging data using the surface representation. The association includes uniquely associating numerical values (e.g., Cartesian coordinates) to particular surface features. The three-dimensional model includes the ability to adjust the pose of the 3D models made from data taken at different times so that the coordinate systems closely correspond.

The invention discloses a preparation method and a time-resolved biological imaging application of thermally activated delayed fluorescent (TADF) organic material-based water-soluble long-life fluorescent nanoparticles. The nanostructure of the nanoparticles is represented by figure 1, a thermally activated delayed fluorescent molecule represented by CzPy is represented by a general formula (I). The method comprises the following steps: synthesizing the pure organic TADF micro-molecular material CzPy through adopting a one-step process, and rapidly injecting a CzPy / lecithin-polyethylene glycol mixed solution to deionized water to obtain the CzPy nanoparticles with good water solubility, high luminescence intensity and long fluorescence life. HELA cell dyeing marking and zebra blood vessel imaging results show that the nanoparticles have the advantages of long fluorescence life, low cytotoxicity and biotoxicity, stable spectrum signal, realization of time-resolved fluorescence imaging in cell or in vivo imaging, and good application prospect.