430results about How to "Fluorescence enhancement" patented technology

Rolling circle replication of a padlock primer is inhibited when it is hybridized to a target nucleic acid that is long or circular. The invention provides methods of addressing this problem including cutting the target nucleic acid near or preferably at the site which hybridizes with the padlock probe, whereby a 3'-end of the cut target nucleic acid acts as a primer for rolling circle replication of the padlock probe. Also included is a method of assaying for a polyepitopic target by the use of two affinity probes each carrying an oligonucleotide tag and of a padlock probe for rolling circle replication in association with the two affinity probes

A process of amplifying a nucleic acid template dependent on partial destruction of primer molecules which have extended onto the template molecule followed by strand invasion of the partially destroyed primer template by a replacement primer. The destruction of the primer molecule may be performed by either endonuclease or exonuclease digestion. A signal generation from the amplified products may be obtained by the use of adaptors capable of binding probe molecules as well as the amplified product.

This invention relates to compositions and electroluminescent (EL) devices that have enhanced performance as a result of a novel class of anthracene derivatives used as host materials for a full range of color dopands. When using coumarin derivatives as color dopands in the anthracene derivatives in an EL device, the device performs a desirable light emitting efficiency and durability. The performance of the EL device can be further improved by using benazole derivatives as the electron transporting layer. The organic EL device of the present invention is useful in preparing display devices.

The present invention provides, in addition to other things, methods, systems, and apparatuses that involve the use of an optical fiber with grating and particulate coating that enables simultaneous heating; optical detection; and optionally temperature measurement. Methods, systems, and apparatuses of the present invention may be used in many applications including isothermal and / or thermal cycling reactions. In certain embodiments, the present invention provides methods, systems, and apparatuses for use in detecting, quantifying and / or identifying one or more known or unknown analytes in a sample.

The present invention relates to materials and methods useful in the study of cells and species associated with cells. Materials of the present invention may include emissive, photostable organic molecules (e.g., conjugated polymers, conjugated oligomers) that are appropriately functionalized to interact with a cell with essentially no disruption in normal cell functioning. The present invention may be useful in, for example, cell imaging, cell tracking, in vivo monitoring of cellular events, drug delivery, and determination of biological species. In one embodiment, a conjugated polymer or oligomer may be internalized into a cell and may exhibit a strong and stable emissive signal, allowing the cell to be monitored under extensive microscopic conditions for extended periods of time.

The present invention relates to metallic-surface detection systems for determining target substances including free bilirubin in neonatal serum in the presence of a predominantly high background of bilirubin bound Human Serum Albumin (HSA) or sensing and isolating target nucleotide sequences wherein a fluorescence signal is enhanced by close proximity of the target substances near metallic surfaces.

Provided are azido-BODIPY compounds of formula (I), cyclooctyne-based fluorogenic probes of formula (IV), and activity-based probes of formula (VI). These compounds undergo azide alkyne cycloadditions (AAC) with to form triazolyl products. The provided compounds are useful for detection and imaging of alkyne-, or azide-containing molecules. Methods for detection and imaging biomolecules using compounds of the present disclosure are disclosed.

The invention belongs to the field of nano material preparation and provides a method to achieve surface-common-enhanced fluorescence and surface-enhanced Raman in a core-shell structure. Spherical silver nano particles are used as substrates of surface-common-enhanced fluorescence and surface-enhanced Raman, the silver nano particles are placed inside the shell layers, with good surface-enhanced Raman activity, of gold nano particles, fluorescent dye molecules are disposed between the silver nano particles and the gold nano particles, and the distances among the silver nano particles, the fluorescent dye molecules and the gold nano particles to achieve surface-common-enhanced fluorescence and surface-enhanced Raman in the core-shell structure. A Stober method is used to change corresponding experiment parameters, silicon dioxide of different thicknesses is wrapped on the surfaces of the silver nano particles to adjusting the distances among the silver nano particles, the fluorescent dye molecules and the gold nano particles, surface-common-enhanced fluorescence and surface-enhanced Raman signals of the manufactured multi-layer core-shell structure composite particles are further enhanced, and the multi-layer core-shell structure composite particles are good in water solubility, uniform and stable in nature, and promising in potential application prospect in fields such as biological imaging, safety detection and catalyzing. The method is wide in raw material source, simple and easy to operate, easy in batch and large-scale production, good in industrial production foundation, and promising in application prospect.

Methods and compositions involving enzyme-activatable fluorophore polymer imaging agents for photodetection of specific tissues and / or human diseases and disorders are provided. In certain embodiments, the imaging agent comprises a hydrophilic polymer backbone (e.g., poly(L)lysine), a hydrophobic fluorophore, and a hydrophilic solubilizing agent. The solubilizing agent may comprise a quarternary ammonium group (e.g., a 1-methylnicotinic group) to enhance self-quenching of the fluorophore. Various methods for the generation and purification of imaging agents are also provided, including methods involving solid phase probe extraction or ion exchange purification.

The invention discloses a method for preparing functional gold nanoparticles with high stability, which belongs to the technical field of material chemistry. The method comprises the following steps of: the DNA-assisted synthesis of the gold nanoparticles, the salt tolerance experiment of the synthesized gold nanoparticles, the representation of the synthesized gold nanoparticles and the biometric identification of the DNA on the surfaces of the gold nanoparticles. In the method, four kinds of DNAs with three different lengths are utilized and a chloroauric acid is reduced by trisodium citrate to realize the assisted synthesis of the gold nanoparticles; the DNAs of 12 basic groups are obtained by performing representation with an electron microscope to assist in synthesizing the uniform spherical gold nanoparticles, and particularly, the gold nanoparticles obtained by the 12-G-assisted synthesis have regular shapes and a uniform particle size; the salt-tolerant effect of the gold nanoparticles obtained by the salt tolerance experiment of the synthesized gold nanoparticles can be bettered by 20 times; and the gold nanoparticles can enhance the fluorescence of FAM-DNA, and are expected to be well applied to the preparation of nanometer medicaments and nanodevices.

The present disclosure describes a method and apparatus for collecting samples of particles from air or other gases at one or more monitored locations, and identifying in real-time whether biological agents, such as bacterial or viral pathogens, are present in the samples. The apparatus preferably uses a liquid-assisted collector to collect the sample of particles, which are suspended in a liquid that contains dyes that detect the presence of nucleic acids. An integrated detector with a light source and a light detector detects whether there is a change in the fluorescence of the liquid, which indicates the presence of a biological agent in the sample.

The present invention provides dyes, reactive dyes and labeled reagents that may be used in the detection or quantification of desirable target molecules, such as proteins and nucleic acids. Dyes are provided that may be used free in solution where the binding of the dye to the target molecule provides signal generation. Dyes are also provided that comprise reactive groups that may be used to attach the dyes to probes that will bind to desirable target molecules. The novel dyes of the present invention have been modified by the addition of charged and polar groups to provide beneficial properties.

The present invention relates to metallic-surface detection systems for determining target substances including free bilirubin in neonatal serum in the presence of a predominantly high background of bilirubin bound Human Serum Albumin (HSA) or sensing and isolating target nucleotide sequences wherein a fluorescence signal is enhanced by close proximity of the target substances near metallic surfaces.

The invention discloses a method for monitoring beta amyloid protein aggregation process by aggregation-induced emission, belonging to the technical field of biomedical research and clinical detection. With the method, the high affinity between sulfydryl on cysteine and maleimide is utilized to specially combine the beta amyloid protein on an aggregation-induced emission probe. The beta amyloid protein aggregation process is monitored on the basis of aggregation-induced emission enhancement phenomenon. The method has the advantages of high sensitivity, high selectivity, good stability, low manufacture cost and the like and is easy to control. The A beta42 and A beta40 contents can be monitored in a micromole, even a nano-mole level, the detection speed of the method is improved by 15 times if being compared with that of the detection method which utilizes probes, such as ANS (8-aniline-1-naphthalene sulfonic acid) and the like. The beta amyloid protein aggregation process can be qualitatively and quantitatively monitored, the method has a good application prospect if being applied to the pathologic diagnosis of latent patients suffering from senile dementia.

The invention provides nano fluorescent particles doping with rare-earth ions. The grain size of the nano particles is 1-200nm, and the nano particles comprise a nano particle matrix and rare-earth ions. The invention also provides a solar battery containing the nano particles.

The present invention provides for mixed metal structures that can be deposited on a substrate or free in solution that exhibit several distinctive properties including a broad wavelength range for enhancing fluorescence signatures. Further, metal surface plasmons can couple and such diphase coupled luminescence signatures create extra plasmon absorption bands. The extra bands allow for a broad range of fluorophores to couple therefore making more generic substrates with wider reaching applications.

The present invention provides novel engineered derivatives of green fluorescent protein (GFP) which have an amino acid sequence which is modified by amino acid substitution compared with the amino acid sequence of wild type Green Fluorescent Protein. The modified GFPs exhibit enhanced fluorescence relative to wtGFP when expressed in non-homologous cells at temperatures above 30° C., and when excited at about 490 nm compared to the parent proteins, i.e. wtGFP. An example of a preferred protein is F64L-S175G-E222G-GFP. The modified GFPs provide a means for detecting GFP reporters in mammalian cells at lower levels of expression and / or increased sensitivity relative to wtGFP. This greatly improves the usefulness of fluorescent proteins in studying cellular functions in living cells.

Provided are azido-BODIPY compounds of formula (I), cyclooctyne-based fluorogenic probes of formula (IV), and activity-based probes of formula (VI). These compounds undergo azide-alkyne cycloadditions (AAC) with to form triazolyl products. The provided compounds are useful for detection and imaging of alkyne-, or azide-containing molecules. Methods for detection and imaging biomolecules using compounds of the present disclosure are disclosed.

A compound represented by the following general formula (I):{R1, R3, R6 and R8 are methyl groups and the like, R2 and R7 are cyano groups and the like, R4 and R5 are fluorine atoms and the like, and X is a group represented by the formula (A) [R11 is a monocarboxy-substituted C1-8 alkoxy group and the like, R12, R14 and R15 are hydrogen atoms and the like, and R13 is hydroxy group and the like],provided that the combination of the aforementioned substituents is such a combination that the compound represented by the formula (I) can be substantially non-fluorescent before reacting with peroxynitrite, and the compound represented by the formula (I) in which the group represented by the formula (A) is nitrated after reacting with peroxynitrite can be substantially highly fluorescent}, or a salt thereof, which is useful as a fluorescent probe for peroxynitrite measurement.

The invention discloses a fluorescent probe, a preparation method thereof and an application of the fluorescent probe for detecting a nucleic acid G-quadruplex structure. The probe is provided with a structure in a general formula (I) is simple and stable in structure and easy to prepare. The probe can be used for specific detection of a nucleic acid G-quadruplex secondary structure, and the G-quadruplex secondary structure in solution can be rapidly detected by a fluorescence spectrometer or direct visual inspection under irradiation of a fluorescent lamp. The probe can be used for detecting the nucleic acid G-quadruplex structure in agarose gel or polyacrylamide gel and can also be used for detecting, marking or displaying existence or distribution of the G-quadruplex structure in living cells. Fluorescent materials have efficient and specific recognition capability for the nucleic acid G-quadruplex structure, have the advantages of excellent cell membrane permeability, low photo-induced toxicity, biological toxicity and photo-bleaching performance and the like, and overcome the shortcomings of high cost, high equipment requirement, complicated technical operation and the like in other detection methods.

Disclosed are nanoparticles having a metallic core consisting essentially of superparamagnetic iron oxide; a polymeric coat surrounding said core, the coat having a matrix of polyacrylic acid and forming an outer periphery of said nanoparticle; a plurality of hydrophobic pockets formed by the polymeric coat; a plurality of carboxylic groups along an outer periphery of the polymeric coat and effective to conjugate with a predetermined targeting ligand which functionalizes the nanoparticle; a lipophylic fluorescent dye encapsulated in the plurality of hydrophobic pockets; and a drug encapsulated in the plurality of hydrophobic pockets. Associated methods of making the nanoparticles and of treatments using the nanoparticles are also disclosed.

The invention relates to preparation and application of a peroxide nitroso (ONOO<->) fluorescent probe based on a hemicyanine dye. A structural formula of the fluorescent probe is shown in the attached figure. The invention provides a preparation method of the fluorescent probe which is synthesized by adopting IR-780, 3-nitrophenol, stannous chloride and the like as raw materials. The fluorescentprobe is characterized in that the fluorescent probe is a ratio peroxide nitroso fluorescent probe with near-infrared effect and high selectivity; firstly, the fluorescent probe has higher sensitivityon ONOO<->, and the specific value of fluorescence intensity (F460 / F708) is enhanced by 153 times; then, the fluorescent probe has higher selectivity on ONOO<->, and is free from the interference byother active oxygen, active nitrogen, active sulfur and biological mercaptan; the fluorescent probe can quickly act with ONOO<->, and the response time is controlled within 80s; the fluorescent probehas been successfully applied into the study of cell imaging, and can be used for detecting the change of content of ONOO<-> in the cells.

The invention relates to a novel water-soluble sulfydryl fluorescent probe which is a 2-(2'-hydroxyphenyl) benzimidazole (HPBI) compound modified by quaternary ammonium salt. The compound has the structure of 2-[5-(trimethyl quaternary ammonium salt)-acetamido-2'-hydroxyphenyl) benzimidazole. The fluorescent probe can be used for detecting a biological sulfhydryl compound in aqueous solution and living tumor cell, i.e. the novel water-soluble sulfydryl fluorescent probe is selectively coordinated with cupric ions and an obtained coordination compound can enter the living cell to be reacted with the sulfhydryl compound, so that a fluorescent compound is changed into ionic molecules again and fluorescent light is recovered. The novel water-soluble sulfydryl fluorescent probe has the advantages that the novel water-soluble sulfydryl fluorescent probe has excellent water solubility and biocompatibility and high sulfhydryl measurement sensitivity; before and after GSH (glutathione) reaction, the fluorescent light is rapidly changed and is stable; the novel water-soluble sulfydryl fluorescent probe can be stored and used for a long time; and according to the fluorescent probe, a good research method is provided for researching a biological signal channel related to the biological sulfydryl and the significance and diagnosis of the sulfydryl in the in vivo process of diseases or organisms.

The invention relates to a preparation method and an application of a ratio type fluorescent probe for peroxynitrite (ONOO<->). The structural formula of the fluorescent probe is shown in the description. The invention provides the preparation method for synthesizing the fluorescent probe from 8-hydroxyjulolidine-9-carboxaldehyde, ethyl acetoacetate and 2-(4-diethylamino-2-hydroxybenzoyl)benzoicacid. The fluorescent probe is a near-infrared ratio type fluorescent probe for peroxynitrite. The fluorescent probe has a very high sensitivity to ONOO<->, and the fluorescence intensity ratio (F500 / F720) is enhanced by 72 times; the fluorescent probe has a very high selectivity to the ONOO<->, and is not interfered by other active oxygen, active nitrogen, active sulfur and biological thiols; and the fluorescent probe rapidly interacts with the ONOO<->, and the response time is within 7 min; and additionally, the fluorescent probe is applied to the detection of the content of peroxynitrite in living cells.

The invention provides a fluorescent probe for intracellular protein labelling as well as a synthesis method and an application of the fluorescent probe. The provided probe is synthesized in simple steps and has good light stability. Compared with existing fluorescent probes for cell labeling, the probe can be specifically bound with SNAP labels in a complicated system and label any protein in cells. The probe can be applied to detection of copper ions in the cells, fluorescence gradually disappears with increase of the concentration of the copper ions, and the copper ion detection function isrealized. The probe realizes labeling of any protein and detection of the copper ions in the complicated environment and has very important application value in the biological and medical fields.

The invention relates to a fluorescent material capable of being effectively stimulated by ultraviolet, purple-light or blue / green LEDs (Light Emitting Diodes) to emit red light as well as a preparation method thereof and a light-emitting device. The chemical general formula of the fluorescent material is MaAbQcOdDe:Rf, wherein M is one or more of Li, Na, K, Mg, Ca, Sr, Ba, Be, Zn, Y, Gd and Ga; A is one element of Li, Na, K and Bi; Q is at least one element of Mo and W; O is an oxygen element; R is at least one element of Eu, Nd, Dy, Ho, Tm, La, Ce, Er, Pr, Sm, Yb, Lu, Sb, Tb and Mn; D is one ion of Cl<->, F<->, Br<->, I<->, NH<4+>, Au<+> and Ag<+>; a, b, c, d, e and f are molar coefficients; a is more than or equal to 0.1 and less than or equal to 5, b is more than or equal to 0.01 and less than or equal to 3, c is more than 0 and less than or equal to 8, d is more than 1 and less than or equal to 32, e is more than or equal to 0 and less than or equal to 1, f is more than or equal to 0.001 and less than or equal to 1, the sum of a, b and f is more than or equal to 0.1 and less than or equal to 9, and 4c is equal to the sum of d and e. the preparation method comprises the following steps of uniformly grinding simple substances, compounds or corresponding salts of M, A, Q, R and D as well as fluxing agents, carrying out high-temperature synthesis, and processing to obtain the fluorescent material. The fluorescent material has the beneficial effects that the range of the excitation wavelength of the fluorescent material is wide (240nm-540nm), the light-emitting efficiency is high, crystals are complete, and the chemical performance is stable; the preparation method is simple, free of pollution, easy to operate and low in cost.

The invention discloses a series of novel 1,8-naphthalimide derivative compounds with triplet state fusion delayed fluorescence (TFDF) properties, and a preparation method and application thereof to electroluminescent devices. The molecules are used as the organic electroluminescent main materials, and can be prepared into high-efficiency yellow, orange and red organic light-emitting diodes; and the utilization rate of the exciton can substantially exceed the theory limit of singlet exciton utilization rate (25%, namely the external quantum yield of 5%). Therefore, the materials are promising main materials with TFDF properties, and the design and synthesis of the materials have important theoretical and practical significance to increase luminous efficiency of electroluminescence devices and promote the industrialization process of the electroluminescence devices.

Image formation via photoinduced fluorescence changes in a polymeric medium with two-photon fluorescence readout of a multi-layer structure. Fluorophore-containing polymers, possessing one or more basic functional groups, underwent protonation in the presence of a photoinduced acid generator upon exposure to a broad-band UV light source or fast-pulsed red to near-IR laser irradiation. Solution studies demonstrated formation of monoprotonated and diprotonated species upon irradiation, each resulting in distinctly different absorption and fluorescence properties. The fluorescence of the original, neutral, fluorophore was reduced upon monoprotonation, leading to a concomitant increase in fluorescence at longer wavelengths due to the monoprotonated form, the basis for multichannel data readout. Experiments in polymer films demonstrate the changes in fluorescence properties of the photosensitive polymer compositions and polymers can be employed for a high storage density, write-once read-many (WORM) data storage medium with two-photon fluorescence readout. Two-channel, two-photon fluorescence imaging provided both “positive” and “negative” image readout capability.