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Method for preparing functional gold nanoparticles with high stability

A gold nanoparticle, high-stability technology, applied in the field of material chemistry, can solve problems such as inability to form crystal nuclei, and achieve the effects of improved salt resistance, uniform particle size, and regular shape

Inactive Publication Date: 2011-03-23
JIANGNAN UNIV
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Problems solved by technology

However, hydroxylamine has been shown to exist only as a reducing agent and cannot form nuclei

Method used

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  • Method for preparing functional gold nanoparticles with high stability
  • Method for preparing functional gold nanoparticles with high stability
  • Method for preparing functional gold nanoparticles with high stability

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Embodiment Construction

[0044] (1) DNA-assisted synthesis of gold nanoparticles

[0045] Preparation of chloroauric acid aqueous solution: Dissolve 1 g of chloroauric acid in 500 mL of double-distilled water once to prepare a solution of about 5 mM. Nanogold was prepared by reducing chloroauric acid with trisodium citrate. Both DNA and FAM-DNA used in the present invention were purchased from Shanghai Sangon Bioengineering Co., Ltd., China, and purified by polyacrylamide gel electrophoresis. The glass instruments used were soaked in strong acid, washed with double distilled water, and dried for later use.

[0046] The specific steps for the DNA-assisted synthesis of gold nanoparticles are: add 1.9 mL of Milli-Q ultrapure water to a clean glass bottle, then add 0.1 mL of chloroauric acid solution (concentration 5 mM), and mix at 37°C. Then add 20 μL of 50 μM DNAs of different lengths and sequences to the mixed system (various DNAs were tested separately). Stir and mix at 37° C. for 15 min, and then...

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Abstract

The invention discloses a method for preparing functional gold nanoparticles with high stability, which belongs to the technical field of material chemistry. The method comprises the following steps of: the DNA-assisted synthesis of the gold nanoparticles, the salt tolerance experiment of the synthesized gold nanoparticles, the representation of the synthesized gold nanoparticles and the biometric identification of the DNA on the surfaces of the gold nanoparticles. In the method, four kinds of DNAs with three different lengths are utilized and a chloroauric acid is reduced by trisodium citrate to realize the assisted synthesis of the gold nanoparticles; the DNAs of 12 basic groups are obtained by performing representation with an electron microscope to assist in synthesizing the uniform spherical gold nanoparticles, and particularly, the gold nanoparticles obtained by the 12-G-assisted synthesis have regular shapes and a uniform particle size; the salt-tolerant effect of the gold nanoparticles obtained by the salt tolerance experiment of the synthesized gold nanoparticles can be bettered by 20 times; and the gold nanoparticles can enhance the fluorescence of FAM-DNA, and are expected to be well applied to the preparation of nanometer medicaments and nanodevices.

Description

technical field [0001] The invention discloses a method for preparing high-stability and functionalized gold nanoparticles, which belongs to the technical field of material chemistry. Background technique [0002] In recent years, due to the unique properties of gold nanoparticles in optics, chemistry, electronics, catalysis and surface-enhanced Raman scattering spectroscopy, their preparation and application have increasingly aroused the research interest of many scientists. Through the further utilization of these properties of gold nanoparticles, the self-assembly of gold nanoparticles, high-specificity selective catalysis, synthesis of crystal materials, and sensitive sensors are applied. [0003] So far, there have been many different preparation methods for the preparation of gold nanoparticles, such as trisodium citrate reduction method, sodium borohydride reduction method, trisodium citrate tannic acid reduction method, and seed crystal growth method. The reduction ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F9/24C12Q1/68G01N21/64
Inventor 胥传来徐丽广马伟陈伟朱颖越赵媛屈昌隆严文静徐乃丰
Owner JIANGNAN UNIV
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