The invention discloses a
cell nucleus DNA staining method. The method comprises the following steps: preparing an AF stationary liquid, a
hydrolysate, a rinsing liquid and an
eosin staining liquid; preparing a
cell DNA staining solution which is composed of a
cell DNA staining solution A and a cell
DNA staining solution B in a volume ratio of 1: 1; placing a
pathological sample into the AF stationary liquid to be fixed; washing the sample with running water, and then placing the sample into a
hydrolysate to hydrolyze; washing the sample with running water, and then placing the sample in the cell
DNA staining solution for staining; washing the sample with running water, and then placing the sample in a rinsing liquid for rinsing; and carrying out dehydrating with
ethanol step by step, placing the sample into an
eosin staining solution, and carrying out decolorizing with absolute ethyl
alcohol, airing, and sealing. According to the method,
dyeing time is greatly shortened, the
hydrolysate is composed of non-precursor chemicals and belongs to an environment-friendly
reagent, compared with a traditional stationary liquid, the AF stationary liquid does not include
acetic acid and lesssimulates the
human body, and the cell
DNA staining solution is simple to prepare, does not need to be heated and boiled, has the validity period longer than 18 months, is ready-to-use and is suitablefor commercial popularization.