104 results about "Spermatogenesis" patented technology

This invention provides a class of androgen receptor targeting agents (ARTA). The compounds are selective androgen receptor modulators (SARM) useful for, inter-alia, suppressing spermatogenesis, treating a subject having a hormone related condition, treating a subject suffering from prostate cancer, delaying the progression of prostate cancer, preventing the recurrence of prostate cancer, and treating the recurrence of prostate cancer.

Methods and compositions for inhibiting or preventing spermatogenesis in a male mammal. Also disclosed are compounds and formulations for use in such methods.

A formulation for male contraception comprising a progestin possessing both estrogenic and androgenic properties is remarkably effective for spermatogenesis suppression in males. The progestin Norethisterone (NET), particularly its derivatives Norethisterone acetate and Norethisterone enanthate in sufficient doses induce oligozoospermia or azoospermia in males. Formulations further comprising an androgen, such as a testosterone derivative such as a testosterone ester, particularly testosterone undecanoate, are especially effective male contraceptive formulations.

This invention relates to sustained release compositions, and methods of forming and using said compositions, in particular for the sustained release of Follicle Stimulating Hormone (FSH). The sustained release compositions comprise a polymeric matrix of a biodegradable biocompatible polymer and stabilized FSH. The method of the invention for forming a sustained release composition includes, dissolving a biodegradable biocompatible polymer in a polymer solvent to form a polymer solution; adding biologically active stabilized FSH; removing the solvent; and solidifying the polymer to form a polymer matrix containing stabilized FSH dispersed therein. Also described is a method for providing a therapeutically effective amount of stabilized FSH in a patient in need of for a sustained period comprising administering to the patient a dose of the sustained release compositions of the invention. The sustained release composition of FSH can be used to promote maturation of follicles, promote spermatogenesis and to treat fertility disorders.

This invention provides FSH analogues having increased serum half-life relative to FSH. This invention also provides related compositions and methods for increasing fertility, egg production and spermatogenesis in a subject.

Novel compounds useful for inhibiting spermatogenesis and cancer treatment, and in particular as inhibitors of heat shock proteins and / or elongation factor 1 alpha.

This invention provides FSH analogues having increased serum half-life relative to FSH. This invention also provides related compositions and methods for increasing fertility, egg production and spermatogenesis in a subject.

The invention discloses an animal sex control method based on Rbmy gene editing and application. The method controls animal sex by using CRISPR / Cas9 system to specifically cut Rbmy gene on Y chromosome. Rbmy gene encodes a germ cell specific nucleoprotein, this protein is an important factor related to spermatogenesis, and by cutting Rbmy gene, it is possible to deactivate Rbmy gene, thus Y sperm or fertilized ovum with Y sperm is deactivated and cannot develop into a normal embryo, birth ratio of female animals is finally increased and sex control is achieved. Therefore, sex ratio of animal offspring can be controlled through the method so that the ratio of female animals with better economic value is increased and production efficiency and economic effect are greatly improved.

The present invention relates to compounds capable of acting as androgen receptor, antagonists, pharmaceutical formulations containing the same, and methods of use thereof. Such uses include, but are not limited to, use as antitumor agents, particularly for the treatment of cancers such as colon, skin and prostate cancer and to induce androgen receptor antagonist activity in a subject afflicted with an androgen-related affliction. Examples of androgen-related afflictions include, but are not limited to, baldness, hirsutism, behavioral disorders, acne, and uninhibited spermatogenesis wherein inhibition of spermatogenesis is so desired.

The invention relates to Chinese herbal medicine feed additive for stud rams, which mainly solves the problem that the sperm quality of stud rams is reduced due to centralized and high-intensity sperm collection in the prior art. The Chinese herbal medicine feed additive is prepared by crashing and mixing epimedium, actinolite, Chinese dodder seed, raspberry, Chinese magnoliavine fruit, astragalus root and dangshen and can be added into feed for usage. The invention improves ram sexual appetite, prompts spermatogenesis, increases sperm motility, effectively improves reproductive performance, solves the problem that the sperm quality of stud rams is reduced due to centralized and high-intensity sperm collection in the prior art according to spermatogenesis principle.

The invention relates to a medicine for treating male oligozoospermia and improving semen quality. Various natural edible plant medicines such as Rhizoma Polygonati, Fructus Lycii, Cordyceps militaris (L.) Link., Fructus Rubi, Fructus Mori, Fructus Hippophae, Semen Coicis, Radix Ginseng, and Concha Ostreae (calcined) serve as raw materials, are combined suitably, and jointly have effects of invigorating the spleen and replenishing Qi, and nourishing the kidney and replenishing essence, Lepidium Meyenii contains macaenes and macamides which are used as active substances has effects of promoting sexual function, increasing the number of sperms and improving activity of the sperms, selenium yeast, aminoacid, L-carnitine tartrate, calcium carbonate and fructose can strengthen and protect energy metabolism of the sperms, taurine and vitamin effectively remove reactive oxide species and protect activity of the sperms, and folacin can promote maturity of the sperms and improve functions of the sperms. The medicine is reasonable in composition, and the synergistic effect of various components is utilized, so that the medicine is remarkable and lasting in treatment effect, safe and reliable and convenient to take orally, and can effectively promote spermatogenesis, improve survival rate of the sperms and improve activity of the sperms.

The invention discloses a traditional Chinese medicine additive for a ram and a using method thereof. The traditional Chinese medicine additive comprises herba epimedii, cistanche, semen cuscutae, morinda officinalis, hawthorn, pine needle, codonopsis pilosula, astragalus membranaceus, bighead atractylodes rhizome and liquorice. The formula has the effects of invigorating spleen to promote digestion, promoting appetite, enhancing the body immunity function, improving the sexual desire of the ram, promoting spermatogenesis, improving sperm activity and effectively improving reproductive performance.

The invention belongs to the field of gene engineering and reproductive medicines, and discloses a seminal plasma micro ribonucleic acid marker related to spermatogenesis deficiency and application thereof. The marker is selected from one or more of SEQ ID No.2, SEQ ID No.3 and SEQ ID No.4, has specificity and sensitivity to the spermatogenesis deficiency, can be used for preparing a reagent for diagnosing or monitoring the spermatogenesis deficiency, can avoid invasive diagnosis, can carry out repeated detection, and is easy to dynamically monitor the degree of the spermatogenesis deficiency.

The invention discloses a ginseng and pilose antler tea seed polypeptide lozenge. The tea seed polypeptide lozenge is characterized in that the tea seed polypeptide lozenge is composed of the following raw materials, by weight, 1300-1500 parts of tea seed meal, 4-6 parts of ginseng, 5-7 parts of pilose antler, 40-50 parts of edible fungus powder, 200-240 parts of peanut powder, 2-4 parts of okra pollen, 100-120 parts of bitter apricot kernel powder, 8-12 parts of jujube kernels, 6-8 parts of prepared rehmannia roots, 12-16 parts of sodium carboxymethylcellulose, 6-8 parts of magnesium stearate and a proper amount of neutral proteases. In the method, firstly, tea seed proteins are extracted from the tea seed meal, then the tea seed proteins are hydrolyzed to generate substances such as polypeptides, amino acids and the like, the utilization rate of tea seeds is raised, the economic benefit is raised, and the prepared polypeptide product has good functions of antioxidation, cholesterol reduction and the like. The added components such as ginseng, pilose antler and the like have health care functions of invigorating spleen, reinforcing lung, promoting spermatogenesis, benefiting blood and the like.

The invention provides a kit for male azoospermia diagnosis and a use method. The kit contains biological marker detection reagents for measuring different expression levels of plasma miR-15b, and azoospermia is prompted by excessive expression of the miR-15b. Tiny RNA (ribonucleic acid) genes miR-15b playing an important role in the sperm generation process are discovered, the miR-15b is differently expressed in male infertility patients, and significant expression change of the miR-15b can be detected in the plasma. Based on the principle of quantitative PCR (polymerase chain reaction), and the kit is fine in result repeatability, objective, accurate and easily popularized in different detection mechanisms, hospital laboratories, male laboratories and the like.

The invention discloses a seminal plasma piRNA marker combination reflecting male sperm activity and judging reproductive dysfunction and application thereof. Four piRNA markers include DQ570956, DQ571813, DQ575659 and DQ575661. The combination can be used for detecting sperm activity and predicting reproductive dysfunction caused by low sperm activity. Seminal plasma is easy to obtain, other tissue is not needed, and noninvasive examination is realized; seminal plasma piRNA can reflect pathological and physiological conditions of the whole spermatogenesis stage, detection accuracy can be improved by taking the seminal plasma piRNA as an auxiliary detection index, the pathological and physiological conditions in the whole spermatogenesis stage are reflected on the molecular level, and a potential target is provided for diagnosis and treatment of male reproductive dysfunction; a numerical value is calculated for each testee by a danger coefficient mathematical calculation method, and sperm activity can be reflected directly and visually through the numerical values, so that interference caused by subjective factors and lack of experience is avoided.

This invention provides a novel class of androgen receptor targeting agents (ARTA). The agents define a new subclass of compounds which are tissue-selective androgen receptor modulators (SARM), which are useful for oral testosterone replacement therapy, male contraception, maintaining sexual desire in women, treating prostate cancer and imaging prostate cancer. These agents have an unexpected in-vivo activity for an androgenic and anabolic activity of a nonsteroidal ligand for the androgen receptor. These agents may be active alone or in combination with progestins or estrogens. The invention further provides a novel class of nonsteroidal agonist compounds and methods of binding an androgen receptor, modulating spermatogenesis, treating and imaging prostate cancer, and providing hormonal therapy for androgen-dependent conditions.

The invention discloses the FSH receptor special bonding area polypeptide, expression vector, the preparation method and their pharmaceutical application. Wherein, the polypeptide has amino acid sequence shown as SEQ ID No.2, SEQ ID No.4 or SEQ ID No.6, and can restrain spermatogenesis safely without effect to androgen and as the gene vaccine for male contraceptive.

The invention discloses a Chinese medicine composition for curing oligospermia and asthenozoospermia and a preparation method of Chinese medicine composition and relates to the technical field of Chinese herbal medicines. The Chinese medicine composition comprises, by weight, 10-15 grams of Chinese wolfberry fruits, 3-8 grams of rehmannia glutinosa, 10-20 grams of Chinese dodder seeds, 5-15 grams of dogwood, 5-15 grams of raspberry, 5-15 grams of cistanche, 10-20 grams of polygonum multiflorum, 10-20 grams of red-rooted saliva roots, 10-20 grams of herba epimedii, 5-15 grams of pericarpium citri reticulatae, 5-15 grams of codonopsis pilosula, 5-15 grams of rhizoma atractylodis macrocephalae, 3-10 grams of liquorice, 5-15 grams of rhizoma polygonati, 5-15 grams of angelic sinensis and 5-15 grams of poria cocos. The Chinese medicine composition can effectively promote spermatogenesis, increase survival rate of sperms and sperm activity, is used for curing the oligospermia and the asthenozoospermia resulted from pathogenic reasons, is particularly suitable for idiopathic oligospermia or asthenospermia, and simple, safe, convenient and effective to take.

A formulation for male contraception comprising a progestin possessing both estrogenic and androgenic properties is remarkably effective for spermatogenesis suppression in males. The progestin Norethisterone (NET), particularly its derivatives Norethisterone acetate and Norethisterone enanthate in sufficient doses induce oligozoospermia or azoospermia in males. Formulations further comprising an androgen, such as a testosterone derivative such as a testosterone ester, particularly testosterone undecanoate, are especially effective male contraceptive formulations.

The present invention relates to intranasal testosterone gels for the controlled release of testosterone into the systemic circulation of males and females for providing constant effective testosterone blood levels, without inducing undesired testosterone spike in blood levels, following pernasal administration. The intranasal testosterone gels of the present invention are safe, convenient to use, well tolerated, stable and easily and reproducibly manufactured on scale up. Moreover, because supra- normal and sub-normal testosterone blood levels are believed to be essentially kept to a minimum or avoided and the testosterone serum levels are believed to remain essentially constant during dose life, i.e., the intranasal testosterone gels of the present invention mimic or restore testosterone blood levels to normal physiologic daily rhythmic testosterone levels, the novel intranasal testosterone gels of the present invention are uniquely suited for testosterone replacement or supplemental therapy and effective for treating males diagnosed with, for example, male testosterone deficiency, such as, low sexual libido, low sexual drive, low sexual activity, low fertility, low spermatogenesis, aspermatogenesis, depression and / or hypogonadism, and females who are diagnosed with, for example, female sexual dysfunction, such as, low sexual libido, low sexual drive, low sexual activity, low amygdala reactivity, low sexual stimulation, hypoactive sexual desire disease ("HSDD"), female sexual arousal disorder and / or anorgasmia. The present invention also relates to methods and pre- filled multi-dose airless applicator systems for pernasal administration of the nasal testosterone gels of the present invention.

A Mettl3-knockout spermatogenesis disorder animal model and a building method thereof are provided. The method includes building a Mettl3 conditionally gene-knockout rodent by applying a CRISPR / Cas9 technique; and mating the Mettl3 conditionally gene-knockout rodent and a rodent of the same kind with germ cells capable of specific expression of Cre so as to obtain a Mettl3-knockout spermatogenesisdisorder rodent model with germ cell specificity. The model built provides new ideas and new means for the cause diagnosis, prevention and treatment of human male infertility and prevention and reduction of paternal birth defects.

The invention discloses a traditional Chinese medicine composite for treating immune thrombocytopenic purpura. The traditional Chinese medicine composite is prepared from the following raw materials in parts by weight: 10-30 parts of cornu bubali, 10-30 parts of indigo naturalis, 10-30 parts of lithospermum, 10-30 parts of pseudo-ginseng, 10-30 parts of selaginella tamariscina, 10-30 parts of angelica sinensis, 1-5 parts of ginseng, 10-30 parts of astragalus mongholicus, 10-30 parts of rehmannia root, 1-5 parts of antler slices, 10-30 parts of tortoise shell, 1-5 parts of placenta hominis, 10-15 parts of radix salviae miltiorrhizae, 10-30 parts of polygonum cuspidatum and 10-20 parts of cortex moutan. The traditional Chinese medicine composite is prepared into powder, pills, pastes or tablets by weighing the raw materials in the weight proportion and using a conventional traditional Chinese preparation method. The traditional Chinese medicine composite is high in components and is quantitatively blended; the dosage form can be powder, pills, pastes or tablets; and the medicines are matched with each other, the composite has the functions of replenishing qi to invigorate the spleen, governing the blood, tonifying kidney to perform spermatogenesis and culture the blood, promoting blood circulation to remove blood stasis to generate new blood, so that the human immunocompetence can be obviously improved, the micro-circulation is improved, the blood cells are increased, and the worries and the poor reaction of the patients caused by hormonotherapy of western medicine and spleen cutting are improved.

Herein are disclosed methods for producing female sperm by incorporating a female's chromosomes into a sperm cell via mostly natural spermatogenesis processes. The methods include transplanting (altered) female stem cells or (altered) cloned germ cells into sterilized testes of a male. Diploid female stem / germ cells are altered in two ways—transdifferentiation to facilitate the expression of spermatogenesis factors (for example, using artificial chromosomes) and / or retrodifferentiation to increase pluripotency and imprinting erasures. The altered cells are transplanted into (artificial) male testes to develop into sperm, which are used to fertilize an egg of a second female, or an egg of the original female. Also disclosed herein are methods for producing male eggs by adding an extra X chromosome to an adult male's germ cells, and cultivating the germ cells in vitro.

The invention discloses a polysaccharide complex of cordyceps sinensis and snow lotuses for tonifying kidney and improving spermatogenesis. The polysaccharide complex of the cordyceps sinensis and snow lotuses for tonifying the kidney and improving the spermatogenesis comprises the following components in parts by weight: 45 to 55 parts of a cordyceps polysaccharide complex, 8 to 12 parts of oyster peptides, 8 to 12 of a rhizoma polygonatum extract, 6 to 10 parts of venison powder, 6 to 8 parts of snow lotuses, 4 to 8 parts of sea cucumber peptides, 8 to 10 parts of medlar, and the balance ofmedical or food health auxiliary materials. According to the invention, efficacies of the rhizoma polygonatum extract, oyster peptides, venison powder, snow lotuses and sea cucumber peptides are further enhanced through the particularity of the cordyceps polysaccharide complex; the cordyceps polysaccharide complex is in compatibility with the oyster peptides, medlar, rhizoma polygonatum, venison powder, snow lotuses and sea cucumber peptides to achieve the functions of nourishing yin, tonifying the kidney and improving the spermatogenesis, and in practice, the cordyceps polysaccharide complexcan effectively tonify the kidney, improve the spermatogenesis and enhance kidney functions of men and women to enhance the sexual abilities of men and women. In addition, the polysaccharide complex uses raw materials of homologous raw materials of medicines and food, and adopts the highest safety level, namely the food raw materials which can be used without limitation are in reasonable compatibility, so that the polysaccharide complex has both the efficacies and safety, and no side effects and dose limit.

The invention relates to a traditional Chinese medicine composition for treating vertigo, which is composed of the following traditional Chinese medicine raw materials in parts by weight: 10-40 parts of astragalus, 5-30 parts of angelica, 5-25 parts of Gastrodia elata, 10-40 parts of Codonopsis pilosula, 5 parts of Fushen ~30 servings, 5-25 servings of polygala, 5-25 servings of yuan meat, 10-40 servings of fried jujube kernels, 10-40 servings of dragon teeth, 5-30 servings of night vine, 5-25 servings of taro meat, 5-25 servings of yam 30 parts, Rehmannia glutinosa 10-40 parts, Guizhi 5-25 parts, Aconite 5-20 parts, Cuscuta 5-30 parts, Angelica dahurica 5-20 parts, Schisandra chinensis 5-25 parts, mulberry octopus 5-30 parts, roses 5 to 25 servings. The composition of the invention has the effects of calming the liver and quenching the wind, removing phlegm and reducing turbidity, nourishing the heart and spleen, nourishing the liver and kidney, and benefiting qi and generating essence. It has a remarkable and reliable curative effect on treating vertigo. Advantages of toxic side effects.