206 results about "Sperm quality" patented technology

The invention discloses a microfluidic biological chip sperm quality analyser, which comprises a biological chip and a measurement host. The biological chip comprises a sample input module and a microchannel. The measurement host comprises a chip butt module, a fluid driving module, a signal detection processing module and a result display recording module. Driven by the fluid driving module, a sperm sample flows at a set flow velocity and in a set mode in the microchannel of the biological chip, and the sperm amount, the sperm concentration and the sperm motility in the tested sperm sample are obtained by the coulter principle and a unique algorithm. The invention also provides a sperm quality analysis method. The analyser of the invention realizes fully automatic high-throughout rapid analysis of the sperm sample. A user only needs to insert the biological chip comprising the sample into the host, then the analyser can rapidly output a test result report. The analyser has simple and convenient operation and low testing cost and is suitable for medical establishments at all levels and households.

The kidney invigorating and yang strengthening Chinese medicine preparation is prepared with morinda root, malaytea scurfpea fruit, cnidium fruit, pilose antler, ursine seal's penis and testes and other 16 kinds of Chinese medicinal materials. It has the function of mildly reinforcing kidney yang, strengthening sexual function, raising sperm quality, etc.

The present invention discloses one kind of complete feed for stud boar. The complete feed consists of corn 65-85 wt%, bran 4-8 wt%, soybean oil 1-1.5 wt%, soybean dregs 10-15 wt%, stone powder 0.5-1.5 wt%, light calcium carbonate 0.5-1.5 wt%, salt 0.1-0.5 wt%, lysine 0.05-0.15 wt%, choline 0.05-0.15 wt%, phytase 0.01-0.03 wt%, additive 0.05-0.1 wt%, sow vitamins 0.05-0.1 wt%, and sow minerals 0.1-0.4 wt%. The complete feed can provide stud boar with sufficient energy, protein, vitamins and minerals and make stud boar possess robust body, hearty sexuality and high sperm quality.

The present invention relates to compositions and methods for the handling of processed sperm including samples that are freshly collected, those transported as fresh samples, samples that are frozen and thawed, those sorted into one or more subpopulations, and those that are otherwise processed or handled that impose trauma on the cell. Trauma can reduce the motility, fertility, viability and overall integrity of the sperm and reduce the ability to fertilize, produce an embryo and a healthy offspring. The present invention relates to novel compounds that can be added to the sperm cell sample to reduce the traumatic effects of physical stress during mild as well as extensive sperm cell processing, methods of using the compounds in standard sperm processing procedures, the end products made from these methods including sperm and embryos, as well as methods of using those end products in assisted reproductive biology techniques in animals.

The invention relates to Chinese herbal medicine feed additive for stud rams, which mainly solves the problem that the sperm quality of stud rams is reduced due to centralized and high-intensity sperm collection in the prior art. The Chinese herbal medicine feed additive is prepared by crashing and mixing epimedium, actinolite, Chinese dodder seed, raspberry, Chinese magnoliavine fruit, astragalus root and dangshen and can be added into feed for usage. The invention improves ram sexual appetite, prompts spermatogenesis, increases sperm motility, effectively improves reproductive performance, solves the problem that the sperm quality of stud rams is reduced due to centralized and high-intensity sperm collection in the prior art according to spermatogenesis principle.

The invention discloses a medicine for treating male oligospermia and asthenozoospermia and improving sperm quality. The medicine for treating male oligospermia and asthenozoospermia and improving sperm quality takes L-carnitine, maca, corduceps militaris, zinc-rich selenium-rich whole egg powder, vitamin C, citric acid, zizyphus jujube, deer blood, folic acid, taurine, fructose and coenzyme Q10 as active compositions, and is prepared according to a certain weight ratio. The medicine aims at the disadvantages of conventional spermatogenic medicines, and is reasonable in formula. By properly combining multiple bulk drugs and utilizing the mutual synergic effect of the bulk drugs, the treatment effect is improved, the medicine adapts to treatment requirements of complex disease conditions, adverse reaction is reduced, and the medicine is durable in treatment effect, fast in absorption, rapid in effectiveness, strong in effect, free of stimulation, safe, reliable and simple for administration, is capable of effectively promoting generation of sperms, improving the survival rate of sperms, and is an effective medicine for treating oligospermia, asthenozoospermia and sperm quality badness caused by various reasons. The total effective rate of the medicine reaches 95.6% based on 5730 examples treated by using the medicine.

The invention relates to a feed for promoting estrus of boars, which belongs to the technical field of production of feeds for pigs and is used for promoting the estrus of boars and improving the boar sperm quality. The feed for promoting the estrus of the boars and improving the boar sperm quality can be used for improving the reproduction performance of boars of child-bearing age. The feed for promoting the estrus of the boars consists of a conventional feed and Chinese herbal medicines and is characterized in that the conventional feed consists of a premix feed consisting of corns, bean pulp, imported fish meal, corn gluten meal, bran, wheat middling, soybean oil, calcium hydrophosphate, mountain flour, salt, lysine, choline chloride and composite vitamins for boars, a composite microelement premix feed and cysteamine; the Chinese herbal medicines include fenugreek grass, actinolite, prepared rehmannia root, cynomorium songaricum and longspur epimedium. According to the feed, on the basis of an optimal formula, the medicine property of the Chinese herbal medicines is sufficiently utilized, the reproduction organism functions of the boars are improved, the estrus of the boars is promoted, the sperm quality of the boars and the reproduction performance of the boars of child-bearing age are improved and the economic benefit is increased.

The invention relates to a medicine for treating male oligozoospermia and improving semen quality. Various natural edible plant medicines such as Rhizoma Polygonati, Fructus Lycii, Cordyceps militaris (L.) Link., Fructus Rubi, Fructus Mori, Fructus Hippophae, Semen Coicis, Radix Ginseng, and Concha Ostreae (calcined) serve as raw materials, are combined suitably, and jointly have effects of invigorating the spleen and replenishing Qi, and nourishing the kidney and replenishing essence, Lepidium Meyenii contains macaenes and macamides which are used as active substances has effects of promoting sexual function, increasing the number of sperms and improving activity of the sperms, selenium yeast, aminoacid, L-carnitine tartrate, calcium carbonate and fructose can strengthen and protect energy metabolism of the sperms, taurine and vitamin effectively remove reactive oxide species and protect activity of the sperms, and folacin can promote maturity of the sperms and improve functions of the sperms. The medicine is reasonable in composition, and the synergistic effect of various components is utilized, so that the medicine is remarkable and lasting in treatment effect, safe and reliable and convenient to take orally, and can effectively promote spermatogenesis, improve survival rate of the sperms and improve activity of the sperms.

The invention discloses a coated carrier and the application thereof in the method for detecting maturity of sperms and separating mature sperms noninvasively; the coated carrier is a carrier coated by hyaluronic acid, or hyalurate or hyaluronic acid derivatives; the detecting method is as follows: preparing specimens; loading the specimens to the coated carrier and covering with microscopic glass; reacting for 5-60 minutes at room temperature; observing by a microscope and counting; and calculating the combination ratio of hyaluronic acid of active sperms. The separating method is as follows: preparing specimens; loading the specimens to the coated carrier, reacting for 5-120 minutes at the temperature of 18-37 DEG C; and collecting the combined sperms. The coated carrier has the advantages of precise and reliable results, simple and fast operation, low cost and good safety performance, and can be used for estimating sperms quality, sperms maturity and analysis of the causes of sterility disease of mails, and is applicable to assisting reproduction or reproduction research center to safely collect mature and active sperms, so that the goal of improving embryo quality greatly is achieved.

The invention belongs to the technical fields of preparation and application of a genetic mark of pigs, and relates to a genetic mark using SNP of a third exon of a CATSPER4 gene as sperm quality property of pigs. The genetic mark is cloned from a CATSPER4 gene exon 3 of the pigs, and the nucleotide sequence is shown as SEQID NO:3. The invention discloses an SNP site on the CATSPER4 gene exon 3 ofthe pigs and a detection method of an SNP subtype. Allelic gene mutation exists on the CATSPER4 gene exon 3, and the mutation causes AlwNI-RFLP polymorphism. The screening step comprises: extractinggenome DNA from pig blood, seeking the SNP site from sequencing results, determining the incision enzyme of the SNP site, and through primer design, PCR amplification and single nucleotide polymorphism detection, enabling the genotype and the sperm quality property to be subjected to correlation analysis. The screened mark can be used for assisting in selection of the mark of the pigs.

The present invention discloses a swine gender-controlling sperm and preparation method and application thereof, which comprises diluting the original boar semen with the original semen diluent containing seminal plasma, genistein and cow urine phenol, separating the diluted original boar semen by the flow cytometry and recycling the obtained swine gender-controlling sperm into the freezing diluent containing seminal plasma, genistein and cow urine phenol. The method provided by the present invention has simple operation, which can effectively prevent the separation and the cryopreservation of pig X, Y sperm from damaging, thereby improving the sperm quality and the fertilization ability, and prolonging the survival time of sperm, wherein the separated sperm can be used in low dose insemination, so as to reduce the production cost. The method can be widely applied to commercial promotion with broad market prospects.

The invention discloses a special diet edible food formula for improving sperm quality of males and a preparation method of the formula and belongs to the field of special diet edible food. Whey protein, sea cucumber polypeptide, oyster polypeptide, bone collagen peptide, soybean peptide, corn oligopeptide, wheat oligopeptide and casein phosphopeptides are used as basic raw materials, edible and medicinal raw materials including raspberries, fructus lycii, cortex cinnamomi and the like are added, wall-broken piney flower powder, spirulina platensis powder, a roxburgh rose extract, a maca extract, cordyceps militaris and fructooligosaccharide are matched, so that the special diet edible food is formed. The formula product is formed through steps of raw material pretreatment (cleaning, enzymolysis, extraction and the like), crushing, sieving, mixing and the like. The formula components are all food materials, and the prepared product has functions of increasing sperm density, increasingsperm motility rate, reducing sperm aberration rate and improving immunity and fatigue resistance. The product has high safety, has no toxic or side effects, has low fishy smell and bitterness and canbe eaten for a long term.

The invention discloses a natural biological preparation for improving male sperm quality through compound microorganism tradtional Chinese medicine production. The microorganism in the microorganism preparation comprises the following components in percentage by weight: 10-20% of brewer's yeast, 20-30% of lactobacillus acidophilus, 20-30% of lactobacillus plantarum and 20-30% of bifidobacterium. The natural biological preparation has the advantage of improving the male sperm quality; the effective constituents of the traditional Chinese medicines are converted through a microorganism compound fermentation technology, so that the natural biological preparation has the characteristics of being easier to absorb and utilize and remarkable in effect; and the natural biological preparation has the effects of resisting oxidation, scavenging free radicals and improving male sperm quality.

Provided are a diluent and a sperm preservation method using the diluent. The diluent is useful in preservation of sperm with high fertility, and is capable of stably achieving quality control and improving quality of the sperm. Refrigerating or freezing sperm using the diluent for sperm, which includes an aqueous solution containing at least one oligosaccharide selected from the group consisting of a fructo-oligosaccharide, an isomalto-oligosaccharide, a gentio-oligosaccharide, and a galacto-oligosaccharide, can improve the quality of preserved sperm and provide sperm having high fertility, at a reduced cost.

A novel sperm quality assessment device includes a main frame and a test pad. The test pad is encircled by the main frame and includes an exposed introductory portion. The test pad has greater hydrophilicity than the main frame, and includes an MTT reagent. Therefore, the activity pattern of the colored motile sperms can be used to estimate sperm quality, such as motility. The present device has the particular advantage of using paper to reduce manufacturing cost, and is also easy to use.

The invention discloses a double-tube vacuum film sperm separation method and a separation device and mainly relates to the field of cytobiology. The separation device comprises an inner centrifugal tube and a centrifugal tube, wherein the lower part of the inner centrifugal tube is of a cone structure which is opened at the tip; a filter device is mounted in the inner centrifugal tube; an inner tube seal cover is arranged at the orifice at the top of the inner centrifugal tube. By using the device to separate a seminal fluid, a density gradient solution is prepared by adopting a classical density gradient sperm separation method and a sperm lotion and sperms are sequentially dropped onto the filter device, and the inner centrifugal tube after low-speed centrifugal treatment is taken out, so that the inner centrifugal tube a is separated from the centrifugal tube, and the residues in the centrifugal tube are part of alpha% density gradient solution and bottom spermatia. The method and device disclosed by the invention have the beneficial effects that whether the seminal fluid is liquefied or not, seminal plasma and sperms are thoroughly separated through a simple operation; the damage on sperms in the pre-treatment process of the seminal fluid is avoided; the success rate, the recovery rate and the quality of sperms are excellent.

The invention discloses a sperm quality fast detection system and detection method based on a micro-fluidic chip. The system comprises the micro-fluidic chip, a reader and an intelligent terminal, wherein the micro-fluidic chip comprises a micro-fluidic channel unit plate and a substrate; the micro-fluidic channel unit plate is arranged on the substrate; the micro-fluidic channel unit plate comprises an inlet, a micro-fluidic channel, micro cavities and an air channel; one end of the micro-fluidic channel communicates with the inlet; the other end of the micro-fluidic communicates with n micro cavities arranged at equal distance; the air channel and the micro-fluidic channel are positioned on the same plane and are in adjacent arrangement; one end of the air channel is connected with a finger membrane pump; the other end of the air channel is sealed; an umbrella-shaped one-way micro valve is arranged on a trunk path of the air channel; an air overflow hole is formed in the umbrella-shaped one-way micro valve; the micro cavities are adjacent to the air channel; the reader comprises a first lens group, a second lens group, a lens barrel, a focusing screw and a light source; a camera of the intelligent terminal is aligned with the lens barrel of the reader.

The invention discloses a breeding boar feed and a preparation method thereof. The feed is prepared from following raw materials including, by weight, 35-45 parts of wheat, 15-20 parts of corn, 8-16 parts of soybean meal, 4-7 parts of fish meal, 3-5 parts of shells of ruditapes philippinarum, 5-8 parts of dried sheep manure, 3-6 parts of male silkworm moth powder, 2.5-4.5 parts of powder of cotyledon of sesbania cannabina, 2-3 parts of cell-disrupted yeast powder, 1.5-2.5 parts of MACA powder, 2-3 parts of ocimum basilcum, 3-4 parts of fish sauce, 2-3 parts of a smoking solution, 0.4-0.8 parts of red yeast powder, 5-10 parts of powder and residue of canna edulis, 4-8 parts of dried peels of persimmon, 3-6 parts of crushed tea dust, 5-10 parts of waste bacteria sticks of lentinula edodes and 2.5-4.5 parts of modified limestone powder. The feed is rich in high-quality proteins, vitamins and minerals, can provide energy required in growth, generation of sperm, hybridization activation and the like of breeding boars, is good in palatability, is easy to digest, can enable the breeding boars to have good appetite and sexual desire and can improve sperm quality and breeding capability of the breeding boars.

A method of testing sperm quality including obtaining a sample of sperm to be tested; detecting and measuring the testis-specific HspA2 chaperone protein (or, the chaperone protein homologues to HspA2) in human and animal sperm; and determining a sperm quality parameter based upon the chaperone protein, wherein an increased amount of the chaperone protein species indicates a higher sperm quality. The chaperone protein is detected and measured either by binding one or more antibodies specific to the sperm chaperone protein to the sperm and measuring the antibody content or measuring ATP bound to the sperm chaperone protein. In the case of the latter method, the chaperone protein may be detected and measured by measuring ATP bound to the sperm chaperone protein, and such measuring is by chaperone protein-bound and CK-B generated ATP measurement, or by bioluminescence of the chaperone protein bound-ATP.

The invention discloses a sperm quality evaluation method based on flow cytometry, which comprises the following steps of: firstly, commonly dyeing a semipermeable membrane nucleic acid fluorescent dye, a non-semipermeable membrane fluorescent nucleotide dye and a sample to be detected; and then, incubating a fluorescent marker monoclonal antibody and the dyed sample to be detected and carrying out flow cytometric detection to obtain the final detection results,. The fluorescent marker monoclonal antibody can be reacted with a sperm acrosome membrane. The laser excitation wavelength differences between the fluorescent marker monoclonal antibody and the semipermeable membrane fluorescent nucleotide dye and between the fluorescent marker monoclonal antibody and the non-semipermeable membrane fluorescent nucleotide dye are 100-200 nm, and the fluorescent scattering wavelength differences are 75-150 nm. The sperm quality evaluation method has the beneficial effects through one-time detection, a plurality of parameter results can be simultaneously obtained, so that the apoptosis rate, the plasma membrane integrity rate and the survival rate of sperm are obtained, and the detection labor strength is reduced; and the demands on a large batch of sample detection in the clinical can be satisfied.

The invention relates to the technical field of medicaments, in particular to a detection kit for sperms and a check method thereof. The invention discloses the detection kit for the sperms and the check method thereof. Many indexes of the sperms such as pH value, sperm volume, liquefying time, sperm density and the like can be quickly and accurately detected by adopting a sample so as to comprehensively reflect the sperm quality; meanwhile, compared with the traditional method, the method saves much time and has simple operation and convenient use; and the detection kit is purchased in a pharmacy and used at home, and is convenient for a detected person to know many indexes of own sperms.

The invention discloses application of cyanidin-3-O-glucoside in preparing drugs for treating and / or preventing sperm quality reduction caused by cadmium. A phenomenon is found that the cyanidin-3-O-glucoside (C3G) can alleviate sperm quality reduction caused by Cd exposure during adolescence. C3G interference can slow a histone-protamine replacement process during a sperm deformation stage through histone modification and improve a testis antioxidant system to prevent sperms from apoptosis induced by Cd oxidative stress, therefore, a normal spermatogenesis process is maintained, and normal spermatogenesis is ensured.

The invention discloses medicine for fast improving the sperm quality and the sexual function. The medicine is prepared by using medlar, herba epimedii, polygonum multiflorum, salvia miltiorrhiza, cortex acanthopanacis, semen cuscutae, ginseng, liquorice and schisandra chinensis as raw materials through extraction according to a certain weight ratio. The medicine can be prepared into any one ordinary oral dosage form. Clinical observation and laboratory study prove that the medicine has the fast and obvious treatment efficacy on symptoms with smaller sperm quantity, lower vitality, survival rate insufficiency and many defective sperms, has a good effect of promoting the sexual desire and improving the sexual function and has a good heath-care effect on male impotence and premature ejaculation, and through clinical observation, the effect taking speed is high, and the toxic or side effect is avoided.

L-carnitine, an amino acid, improves sperm quality and motility. Ferulic acid, an antioxidant found in Dong quai, also improves sperm quality. Sperm are highly susceptible to free radical or oxidative damage from environmental toxicants and natural aging. Vitamins C and E, green tea, and selenium are all potent antioxidants that help improve overall reproductive health. Zinc and B vitamins (B6, B 12 and folate) are critical nutrients in male reproductive systems for hormone metabolism, sperm formation and motility.

The invention relates to the technical field of breeding, in particular to a wasp breeding method. The wasp breeding method comprises the following steps: (1) cultivating a queen bee; (2) feeding male bees; (3) breeding; (4) overwintering. According to the wasp breeding method, female bees are bred, feed is changed before mating, food materials and traditional Chinese medicines capable of promoting secretion of female hormone are added, the trace elements in the feed are supplemented, sufficient nutrition preparation is provided for mating, the resistance of the queen bee is effectively guaranteed, the survival rate of newly born bee eggs is effectively increased and reaches 98.1 percent, and the survival rate of young eggs after 7 days reaches 97.5 percent; feed is changed for the male bees before mating, the secretion of male hormone is effectively promoted, the sperm number of the male bees is increased, the sperm quality of the male bees is improved, the insemination rate is effectively increased during mating, guarantee is provided for increasing the survival rate of the young eggs, and the sperm number of single male bee reaches 0.95 [mu]L.

The invention provides a sperm collection analysis system, which comprises a sperm collection function and a sperm quality analysis function. The sperm collection device is used for stimulating a vagina environment, the sperm collection strength is controllable, and the sperm collection process is easy and pleasant with massaging, pumping, sucking, vibration and other modes for massaging stimulation, reciprocating stimulation and multimedia video stimulation. Sperm quality analysis comprises a digital microscope system and a computer sperm analysis software system, which are used for analyzing the number of sperms, sperm survival rate classification, movement speed classification and other related parameters. The sperm collection analysis system is integrated with sperm collection and sperm analysis, so that the cleanness and freshness of collected sperms and objective truth of sperm quality analysis data can be ensured. The sperm collection device can be used in prospermia and desensitization exercise.

The present invention relates generally to the fields of reproductive medicine. More specifically, the present invention relates to methods and kits for determining the human sperm quality.

The invention relates to a method for inducing Verasper moseri sperm maturation and enhancing activity by using slow releasing hormone. Sexually mature parent fish is selected, and salmon gonadotropin releasing hormone (sGnRH) is implanted into the back muscle (35 mu g / kg) to promote the sperm to finally become mature and enhance the sperm activity to more than 85-90%, thereby increasing the fertility rate by 4-7 times. The method has the advantages of low reproductive menace for parent fish, high survival rate and sex gland maturation rate of parent fish, and favorable spawning effect, and can obtain massive high quality oosperms. The method is a complete control technique. When the sperm quality starts to degrade, implantation is carried out on the parent fish for the second time, and the repeating effect is good. The method can ensure the large-scale artificial propagation of fries to be smooth.

The invention relates to a making method of an antibiotic-free traditional Chinese medicine type feed for stock boars. The antibiotic-free traditional Chinese medicine type feed is prepared by mixing the following components in a certain proportion: astragalus membranaceus, epimedium, desertliving cistanche, actinolite, cinnamon, radix rehmanniae, prepared rhizomes of rehmannia, Chinese angelica, nutgrass galingale rhizomes, dipsacus roots, semen cuscutae, figwort, radix codonopsis, ferrous sulfate, manganese sulfate, zinc sulfate, calcium iodate, sodium selenite, complex vitamins, lysine, threonine, choline chloride, Ketian, phytase, chlorotetracycline, an antioxidant, table salt, baking soda, dicalcium phosphate, calcium carbonate, zeolite powder, corn, soybean meal, broken rice, rice bran and wheat bran. The antibiotic-free traditional Chinese medicine type feed disclosed by the invention has the beneficial effects that after adult stock boars are fed with the feed provided by the invention, the weight can be maintained, sperm quality can be guaranteed, the sexuality of the boars is promoted, or vigorous sexuality of the boars is maintained, the boars are maintained in the healthy state, and the stocking life (adolescent ) of the boars is prolonged.