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Application of cyanidin-3-O-glucoside in preparing drugs for treating and/or preventing sperm quality reduction caused by cadmium

A technology of cyanidin and sperm quality, which is applied in the field of biomedicine, can solve the problems that Cd has not been reported to induce the improvement of pubertal sperm quality, and the protection mechanism is not cleared, so as to improve the decline of sperm quality, ensure normal production, and improve cell apoptosis. death effect

Pending Publication Date: 2020-02-07
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report about the improvement effect of C3G on Cd-induced decline in pubertal sperm quality in current studies, and its protective mechanism has not yet been clarified

Method used

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  • Application of cyanidin-3-O-glucoside in preparing drugs for treating and/or preventing sperm quality reduction caused by cadmium
  • Application of cyanidin-3-O-glucoside in preparing drugs for treating and/or preventing sperm quality reduction caused by cadmium
  • Application of cyanidin-3-O-glucoside in preparing drugs for treating and/or preventing sperm quality reduction caused by cadmium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0106] Example 1 The protective effect of C3G on mouse testis and sperm

[0107] 1. Grouping of animals

[0108] After being adaptively fed with ordinary AIN93-G feed for 4 days, the mice were randomly divided into three groups according to the weight stratification method: Control group, Cd group, Cd+C3G group, 28 in each group. The Control group was given ordinary AIN93-G feed, and intragastric administration of normal saline every day; the Cd group was given ordinary AIN93-G feed, and daily intragastric administration of 5 mg / kg.bw CdCl2 solution; the Cd+C3G group was given anthocyanin feed diet, and daily gavage Stomach 5mg / kg.bw CdCl 2 solution. Mice in each group were given free access to water and food, and were treated continuously for 30 days. On the 10th day, 20th day, and 30th day, 8, 8, and 12 mice in each group were anesthetized, and their samples were collected for subsequent experiments. In addition, 12 young mice in the 0d group were set up, and they were d...

Embodiment 2

[0127] Example 2 C3G improves Cd-induced spermatogenesis arrest through histone modification

[0128] 1. C3G relieves Cd-induced spermatogenesis arrest

[0129] 1. Experimental method

[0130] The testicular seminiferous epithelium was staged by PAS staining.

[0131] 2. Experimental results

[0132] The results showed that the elongated sperm ES in the lumen of the seminiferous epithelium in stages VII-VIII was significantly reduced after Cd exposure, and the karyotype was destroyed ( Figure 8 ). At the same time, the proportion of late sperm and Sertoli cells in the lumen at different stages of sperm deformation was counted, and it was found that the proportion of late sperm decreased significantly compared with the control group from the 11th to 12th stages after Cd exposure ( Figure 9 ), revealing that pubertal Cd exposure blocks spermatogenesis at the stage of sperm metamorphosis. The results of the intervention showed that after C3G treatment, the phenotype of eac...

Embodiment 3

[0154] Example 3 Protective effect and mechanism of C3G on sperm cell apoptosis mediated by Cd-induced oxidative damage

[0155] 1. C3G inhibits the oxidative stress injury of testicular tissue caused by Cd

[0156] 1. Experimental method

[0157] Cd can cause oxidative damage to the body by inducing oxidative stress, and C3G, as an antioxidant active substance, is a potential compound to inhibit its damage. MDA is a lipid peroxidation end product, and its content represents the degree of lipid peroxidation, so its content was detected.

[0158] 2. Experimental results

[0159] Depend on Figure 18 It can be seen that the content of MDA increased significantly after Cd exposure, while C3G intervention significantly reduced its level. GSH is an antioxidant substance in the body, which can resist the oxidative damage of the body. Depend on Figure 19 It can be seen that the excessive consumption of GSH in the testis decreased after Cd injury, and the ratio of GSH / GSSG decr...

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Abstract

The invention discloses application of cyanidin-3-O-glucoside in preparing drugs for treating and / or preventing sperm quality reduction caused by cadmium. A phenomenon is found that the cyanidin-3-O-glucoside (C3G) can alleviate sperm quality reduction caused by Cd exposure during adolescence. C3G interference can slow a histone-protamine replacement process during a sperm deformation stage through histone modification and improve a testis antioxidant system to prevent sperms from apoptosis induced by Cd oxidative stress, therefore, a normal spermatogenesis process is maintained, and normal spermatogenesis is ensured.

Description

technical field [0001] The invention relates to the technical field of biomedicine, and more specifically, relates to the application of cyanidin-3-O-glucoside in the preparation of medicines for treating / or preventing sperm quality decline caused by cadmium. Background technique [0002] Epidemiological studies have shown that the number and concentration of male sperm have decreased by 50% in the past 40 years, and the infertility rate has reached as high as 15%. Among them, exposure to environmental pollutants is one of the important reasons for the continuous increase in male infertility. Cadmium (Cadmium, Cd) is a heavy metal environmental pollutant that can enter the body through food, water, air and other ways and accumulate, and can cause sperm quality to decline through oxidative damage and other ways. The adolescent male reproductive system is in the developing stage, and it is more sensitive to the damage caused by Cd than the sexual mature stage. Cyanidin-3-O-g...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P15/08A61P39/02
CPCA61K31/7048A61P15/08A61P39/02
Inventor 白卫滨李旭升蒋鑫炜孙建霞田灵敏
Owner JINAN UNIVERSITY
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