63results about How to "Repress transcription" patented technology

The present invention discloses a coronary artery medicine coating scaffold for inhibiting endometrial hyperplasia and preventing restenosis in the scaffold. It includes dilating scaffold base body and inner coating layer coated on the dilating scaffold base body, the above-mentioned inner coating layer is formed from medicine carrier and ligustrazine, their mass ratio is 3:1-1:3, the above-mentioned inner coating layer is one of methyl acrylate, methyl methacrylate copolymer, polylactic acid, polyglycollic acid, polymonoracemic lactic acid, polylactide-diglycolide polymer, phosphorylcholine, ethyl polyurethane inorganic micropore aluminium oxide and cellulose or their composite.

The invention relates to a pTerm-SC plasmid as well as a construction method and application thereof. The pTerm-SC plasmid comprises a repE gene, a sopA gene, a sopB gene, a sopC gene, an ori2 replicon, an oriV replicon, prokaryote transcription terminators and antibiotics resistance genes. The pTerm-SC plasmid has the characteristics of very low copy number, high volume and stability and the like, can be applied to gene engineering fields such as the cloning, the screening, sequencing and the genome construction of complex-structure genes including repetitive-sequence genes, instable genes and long-fragment genes and has important role in the high-efficiency and high-quality synthesis of genes.

The invention discloses an application of improved ATMT (Agrobacterium Tumefaciens-Mediated Transformation) to construction of trichoderma atroviride T23 deltaCrel. The application is characterized in that the genomic DNA of trichoderma atroviride T23 is taken as a template, PCR (Polymerase Chain Reaction) amplification is carried out on the Crel gene sequence, and thus upstream and downstream flanking sequences of the Crel gene are obtained; then leading in respectively to knock out upstream and downstream of plasmid pC1300qh, so as to form recombinant plasmid pC1300qh-deltaCrel; converting escherichia coli competence of the recombinant plasmid pC1300qh-deltaCrel, performing verification, screening positive clone, extracting recombinant plasmid pC1300qh-deltaCrel, leading in agrobacterium tumefaciens AGL1, and carrying out improved ATMT. By using the improved ATMT technique, a trichoderma atroviride carbon metabolism inhibiting factor CRE1-knocked-out mutant strain is successfully constructed; the T23 deltaCrel bacterial strain has the functions of promoting secretion and activity of degrading enzyme of cell walls of trichoderma spp., and indirectly improving the function of trichoderma spp. to antagonize pathogenic bacteria.

The invention discloses a preparation method of a double-response nano prodrug for cancer synergistic treatment. The preparation method comprises the following steps: with cis-platinum as a precursor,performing carboxylation modification and then carboxyl activation on the cis-platinum; mixing the activated carboxyl cis-platinum with L-arginine; and forming a double-response nano prodrug with thecis-platinum/arginine composite structure through dehydration condensation of intermolecular carboxyl and amino. The method has advantages of being simple, green, and mild and being low in cost. Thecis-platinum can effectively inhibit DNA transcription to realize the effect of treating cancer; cis-platinum can promote H2O2 generation under the induction of NOX enzyme in vivo and the arginine serving as human amino acid can react with H2O2 to generate NO, so that tumor extinction is further induced. Therefore, the nano prodrug is expected to be developed into an efficient anticancer drug.

The invention relates to the technical field of biology, in particular to application of lycopene in preparation of antiviral drugs. The lycopene has a new function of inhibiting cysteine protease activity, has a broad-spectrum inhibition effect on viruses containing 3CLpro, and can be used for research and development of antiviral drugs.

The invention discloses antibacterial, worm-expelling and fresh-preserving cotton cloth which is prepared by the steps of: dipping the pure cotton cloth in pepper water with concentration of 1% for at least 10min, and then spraying molecular chitin or chitosan with the amount of 2.5g/sq.m on the cotton cloth after the cotton cloth is dried. The cotton cloth treated by the method is made into various packaging supplies with a sterilizing rate of 60%, a worm-expelling rate of 98% and a valid period of up to half a year. The packaging bag made of the antibacterial, worm-expelling and fresh-preserving pure cotton cloth is safe and non-toxic for packaging cereal products and dried fruits, good for people's health, simple and convenient to make, and worth popularizing.

The invention discloses a novel pesticide for controlling plant bacterial diseases. The novel pesticide is prepared from raw materials in percentage by weight as follows: 1%-10% of berberine sulfate, 1%-10% of a solubilizer, 5%-15% of pesticide adjuvants and 0.1%-1% of a preservative, wherein berberine sulfate is chemically synthesized or extracted and purified from a traditional Chinese medicine, and the molecular formula is C20H18NO8S; the mole ratio of the solubilizer to berberine sulfate is 1:1-2; the novel pesticide for controlling plant bacterial diseases mainly contains 0.5%-1% of berberine sulfate and the balance of the solubilizer, the pesticide adjuvants and water; the novel pesticide adopts a form of a microemulsion, an aqueous solution or an emulsion in water. The application method of the novel pesticide comprises the following steps: the pesticide is diluted to 500-1,000 times solution, and the solution is used for root irrigation or sprayed to rhizome bases of tobacco plants after tobacco seedlings are transplanted for about 7-10 days, is applied once every 8-11 days and is continuously applied 3-4 times. The novel pesticide has wide antibacterial spectrum, does not produce drug resistance easily, is low-toxic for human and livestock, is residue-free and produces little pollution, and the control effect on the plant bacterial diseases such as tobacco bacterial wilt and the like is better than that of agricultural streptomycin.

The invention provides an organic selenium composition which comprises selenium-enriched edible fungus powder, wolfberries, honeysuckle and vitamin E. The organic selenium composition can be used as a food, can also be used as a medicine, can be taken for a long period of time, and is capable of improving the human immunity, preventing tumors, inhibiting growth of cancer cells and synthesis of DNA, RNA and protein, inhibiting oncogene transcription and interfering with metabolism of carcinogenic substances. The selenium-enriched edible fungus powder is matched with the wolfberries, the honeysuckle, the vitamin E and Jerusalem artichoke for use, so that the synergistic effects can be developed, the human immunity is enhanced, especially, the immunity of patients receiving tumor chemotherapy is enhanced, and the chemotherapy effect is strengthened.

The invention belongs to the field of hepatitis B prevention and treatment, and in particular relates to a hepatocyte nuclear factor 4a specificity short hairpin RNA with an inhibition effect for hepatitis B virus replication and applications thereof. The technical problem to be solved by the invention is to provide a novel effective choice for HBV resistant treatment. The technical scheme of the technical problem solved by the invention is to provide a specificity short hairpin RNA aiming at the hepatocyte nuclear factor 4a and a carrier capable of expressing the sequence. The shRNA sequence and the expression carrier thereof provided by the invention are testified to be able to inhibit internal HBV transcription and copy effectively and have no obvious side effects, and provide a novel effective choice for HBV resistance, and have favorable application prospects in preparing HBV-resistant medicaments.

The invention discloses a method for inhibiting geminivirus infection by overexpression of arabidopsis ABI5 protein, which inhibits transcription of geminivirus coding genes by overexpression of plant transcription factor ABI5 protein and combination of ABI5 protein and a promoter of geminivirus through specificity, and realizes improvement of virus resistance of plants.

The invention provides application of xanthohumol in preparation of a SARS-CoV-2 inhibitor. The anti-virus active compound xanthohumol with high binding capacity with a Mpro protease is obtained through structure-based virtual screening by taking the Mpro protease, which causes lung inflammation, of the SARS-CoV-2 as a target point. The xanthohumol is bound with the target Mpro protease to inhibitthe activity of the Mpro protease, so that replication and transcription of viruses are inhibited, and the effect of resisting SARS-CoV-2 infection is achieved. The xanthohumol can be applied to preparation of the SARS-CoV-2 Mpro protease inhibitor so as to be applied to preparation of a medicine for preventing and/or treating SARS-CoV-2 inflammation. The binding energy of the xanthohumol and theMpro protease is -63.427 KJ/mol, the IC50 of the xanthohumol to the Mpro protease is 4.97 +/- 1.79 [mu]M, and the inhibition constant Ki value is 2.14 [mu]M.

The invention discloses a composition for treating testicular atrophy and a preparation method thereof, and relates to the field of medicines for treating testicular atrophy, in particular to a composition for treating testicular atrophy and a preparation method thereof. The invention aims to solve the problem of poor testicular atrophy treatment effect of the existing platelet-rich plasma. The composition comprises a platelet lysis buffer and a BMSCs exosome, and the volume ratio of the platelet lysis buffer to the BMSCs exosome is 1: 1. The platelet lysate sPL and the BMSCs exosome are prepared into the composition for promoting cell regeneration, and the composition is used for improving and treating testicular atrophy of rats. By taking activation and recovery of functions of spermatogonial stem cells as an entry point, a sPL preparation method capable of increasing secretion of three growth factors capable of promoting proliferation of spermatogonial stem cells, namely EGF, bFGF and IGF-1, and a BMSCs culture method for highly expressing miR-486-5p exosomes are found. The medicine is used for treating testicular atrophy.

The invention discloses an application of two phenylethanoid glycoside compounds, namely verbascoside and isoacteoside to preparation of antidiabetic drugs. Monomeric compounds of verbascoside and isoacteoside can be extracted from various plants, so that the source is wide, the toxicity is low, and the stability is good. An inventor proves by experiments that (1) the verbascoside and the isoacteoside can protect and repair an injury of an NIT-1 pancreatic beta-cell caused by alloxan, and promote insulin secretion of the injured pancreatic beta-cell in a dose-dependent manner; (2) a free radical injury of the pancreatic beta-cell caused by the alloxan can be reduced; and (3) the activity of the pancreatic cell can be enhanced, the survival rate of the cell is improved, and the mechanisms of the verbascoside and the isoacteoside can activate transcription of an apoptosis-inhibiting gene Bcl-2 mRNA and inhibit transcription of an apoptosis-promoting gene Bax mRNA and finally inhibit transcription of a Caspase-3 gene and expression of Caspase-3 and Bax proteins, thereby inhibiting apoptosis of the pancreatic cell injured by the alloxan. Therefore, the two phenylethanoid glycoside compounds, namely the verbascoside and the isoacteoside disclosed by the invention have potential value of being developed into antidiabetic candidate drugs.