562 results about "Ovarian follicle" patented technology

The present invention discloses a novel method of prevention of birth defects, miscarriages and infertility in women. The two therapies, disclosed herein, the hormone normalization therapy and the aromatase inhibitor-hormone normalization therapy, focus on restoring young hormonal levels in women in order to prevent female infertility and miscarriages, guide follicular and oocyte maturation, and promote correct chromosomal segregation in oocytes and early embryos.

The invention provides recombinant native and mutein forms of human follicle stimulating hormone beta subunit (FSH beta) with characteristic glycosylation patterns which are influential in the metabolic activity of the protein. The invention also provides recombinant mutant forms of the human alpha subunit common to FSH, LH, CG, and TSH, to obtain hormones which also have unique glycosylation patterns. Also provided are recombinant materials to produce these subunits separately or together to obtain complete heterodimeric hormones of regulated glycosylation pattern.

A method for synchronizing ovulation in sows and gilts by a single injection of hormones is disclosed. A hormone, gonadotropin releasing hormone (GnRH), luteinizing hormone (LH), follicle stimulating hormone (FSH), human chorionic gonadotropin (hCG), analogues, derivatives, agonists or combinations thereof is administered to an open sow post weaning at a specific time to stimulate ovulation of mature responsive follicles. The sow is then bred, without heat detection, at a specific subsequent timed interval after injection with hormone, with one or two artificial or natural breedings. In gilts, the hormone is injected at a timed interval from onset of estrus or at a specific timed interval following Prostaglandin F2a for those gilts which have been held in a state of pseudopregnancy.

The invention discloses a long-acting recombinant human follicle-stimulating hormone-Fc fusion protein (hFSH-Fc) and a preparation method thereof. The recombinant hFSH-Fc is a dimerization fusion protein. An amino acid sequence of the hFSH-Fc comprises an hFSHbeta subunit, CTP (carboxy-terminal peptide), an hFSHalpha subunit, a flexible peptide linker and a human IgG (immunoglobulin G)2 Fc variant from the N terminal to the C terminal in sequence. The hFSH-Fc has longer half-life in vivo and smaller side effects than existing hFSH. The invention also relates to an application of a recombinant hFSH-Fc composition to preparation of drugs for treating and/or preventing infertility.

The invention discloses a long-acting recombinant human follicle-stimulating hormone-Fc fusion protein (hFSH-Fc) and a preparation method thereof. The hFSH-Fc is a dimerization fusion protein. An amino acid sequence of the hFSH-Fc comprises an hFSHbeta subunit, CTP (carboxy-terminal peptide), an hFSHalpha subunit, a flexible peptide linker and a human IgG (immunoglobulin G) Fc variant from the N terminal to the C terminal in sequence. The hFSH-Fc has longer half-life in vivo and smaller side effects than existing hFSH. The invention also relates to an application of a recombinant hFSH-Fc composition to preparation of drugs for treating and/or preventing infertility.

The invention relates to the field of hypothermal medicine of biotechnology, in particular to a cryoprotectant free of DMSO (dimethyl sulfoxide). The cryoprotectant comprises 200 mmol/L of trehalose, 0.3 g/mL of PVP (polyvinylpyrrolidone) 40, 200 mu g/L of cholesterol and 200 mu g/L of lecithin. The cryoprotectant is injected into a newly obtained ovary; all macroscopic follicles on the surface of the ovary are punctured and sucked, so that content in the follicles is removed; then the ovary is put in a freezing bag to be cooled. The cryoprotectant doesn't contain DMSO, does not have cytotoxicity and is easy to remove, simple to prepare and good in cryoprotective effect, raw materials are easy to obtained, the rate of normal primordial follicles is high, and the number of dead cells is small; an ovary cryopreservation method is simple and easy to operate and good in cryoprotective effect; the histological structure can be better conserved after the whole ovary of sheep is frozen.

Methods of regulating angiogenesis, ischemic injury and/or wound healing by modulating the activity of leptin, particularly as mediated by the leptin receptor, and/or the interaction between leptin and the leptin receptor. Correspondingly, these methods can also be used to treat diseases mediated by angiogenesis, including wound healing, tumors and tumor metastasis, diabetic microangiopathy, retinal neovascularization, neovascularization of adipose tissue and fat metabolism, revascularization of necrotic tissue, enhancement or vascularization in microvascular transplants, and ovarian follicle maturation. Assays for identifying agents that modulate leptin and/or leptin receptor-mediated angiogenesis and/or wound healing and their use in treating angiogenesis-mediated diseases or conditions involving wound healing are also disclosed.

Methods and apparatus for interpreting vaginal measurement data by electronic means without the need for the user to take part in the data interpretation process. More specifically, an intelligent probe monitors folliculogenesis and correlates the ovarian function data to probe profile characteristics of the female reproductive cycle. A fertile window is defined with reference to the correlation between folliculogenesis and probe cyclic profiles. Data stored within the apparatus can be downloaded into an external display appliance or data analysis unit. Another aspect of the invention allows for continuous layout of the memory of preceding cycles in the inventory of the apparatus, retaining the most recent while erasing the most distant stored data. These methods assure a reliable fit for the diagnosis of fertility, treatment of infertility as far as timing of treatment procedures, management of subfertility and other gynecological disorders such as luteal phase deficiency or short luteal phase, and the management of premenstrual mood disorders, and of premenopausal and menopausal patients.

The invention relates to the special aquatic product culture technology field, particularly an acrossocheilus fasciatus simulated ecological factory large scale breeding and domestication method. The method comprises the following steps: build an acrossocheilus fasciatus breeding water quality regulation and control pool-build an acrossocheilus fasciatus parent cultivation, oviposition and adult fish domestication pond-build an acrossocheilus fasciatus hatching and fry cultivation culture pond-build an acrossocheilus fasciatus ovarian follicle hatching sieve-make the access to water source-disinfect the acrossocheilus fasciatus parent cultivation, oviposition and adult fish domestication pond-adjust the water level of the acrossocheilus fasciatus parent cultivation, oviposition and adult fish domestication pond-select the parents-oviposition of the parents-ovarian follicle collection-ovarian follicle hatching-fry cultivation-adult fish culture-disease prevention in culture stage. The pure excellent water is used in the whole process. The beneficial effects of the invention are as follows: the sexual gland of the parents grows well because of ecological cultivation; the amount of nature oviposition is huge with big granule and good quality; because of suspension hatching, the outside is dynamic and the inside is static, and the oxygen is enough; the cultivation of the fry in the culture pool is enhanced; the adult fish grows fast because of abundant bait; and similar wild quality is provided because of being similar to nature environment.

The invention discloses a culture method for high-efficiency human follicle stimulating hormone expression CHO cells and belongs to the technical field of bio-pharmaceuticals. According to the culture method, seed cells are prepared into cell suspension, and subculture is performed after resuspension through a serum-free basal culture medium to obtain seed suspension; the obtained seed suspension is inoculated into a bioreactor to be cultured, the serum-free basal culture medium is fed at regular intervals in the culture process, growth situations of the cells are monitored, glucose solution is supplemented, and the cells are harvested after culture. According to the method, the serum-free basal culture medium is used throughout the process, culture in a fermentation tank is performed after reviving and amplification through bottle shaking, the process flow is simple, the cell culture density is high and can be as high as 4*107/mL , the cell survival rate reaches more than 98%, maintaining time under high density and a high survival rate is long, the yield of end products can reach 40mg/L, and the method is applicable to industrial production.

Methods are provided for activating dormant ovarian primordial follicles in a mammal to promote development to preovulatory follicles.

A method for synchronizing ovulation in sows and gilts by a single injection of hormones is disclosed. A hormone, gonadotropin releasing hormone (GnRH), luteinizing hormone (LH), follicle stimulating hormone (FSH), human chorionic gonadotropin (hCG), analogues, derivatives, agonists or combinations thereof is administered to an open sow post weaning at a specific time to stimulate ovulation of mature responsive follicles. The sow is then bred, without heat detection, at a specific subsequent timed interval after injection with hormone, with one or two artificial or natural breedings. In gilts, the hormone is injected at a timed interval from onset of estrus or at a specific timed interval following Prostaglandin F2a for those gilts which have been held in a state of pseudopregnancy.

The invention provides a beef cattle breeding method. The beef cattle breeding method comprises the following steps that firstly, beef cattle of improved variety are selected; secondly, cows are raised; thirdly, a breeding method is conducted, wherein a, estrus induction is conducted on the cows close to the estrus, the cows are observed very carefully early in the morning and at nightfall, and observation is conducted every 4-5 hours in the daytime for estrus situation identification, and an outer observation method and a rectal palpation method for the follicular development degree are adopted as the estrus situation identification method, and b, artificial fertilization is conducted through a rectum grasping semen deposition method at estrus stage III; fourthly, the cows are kept under the precious care as long as pregnancy is confirmed according to the situation that the cows are not in estrus any longer within two to three months after semen deposition, or through rectum pregnancy check so that the cows can breed healthy calves, and if false pregnancy is found, re-copulation measures are taken in real time to reduce the breeding loss. By means of the method, the physique of the cows is improved, and the breeding rate of the beef cattle is further increased through estrus induction.