387results about How to "Promote hyperplasia" patented technology

The invention discloses an industrial production method of hydrolyzed wheat protein for feeding, belonging to the technical field of feed additive production. In the method, through the method of synergy of multiple enzymes and combined treatment of multiple steps, the hydrolyzed wheat protein for feeding is prepared by taking wheat gluten meal and water with weight ratio of 1:10-1:2 and adding compound enzyme, ensuring the mass percent concentration of the compound enzyme in the solution is 0.2-1.4%, hydrolyzing the mixture for 1-13h under the conditions that the temperature is 30-70 DEG C and pH value is 5.0-10.0, then carrying out such processes as filtering, decoloring and drying, etc. Compared with the traditional methods, the method of the invention features mild hydrolysis conditions, sanitation and safety and maintains the original nutritive composition of amino acid free of loss; the hydrolysate is low in salt content, mellow in mouth feel and high in yield which is up to 98%. The tests that the hydrolyzed wheat protein is added in the feed for suckling pigs, chicks, calves, aquaculture and the like to be used show that the hydrolyzed wheat protein has the advantages and evident effects of improving daily feed intake, reducing diarrhea rate, enhancing immunity, slowing weanling stress, improving growth performance and the like.

The invention discloses coarse cereal food subjected to treatment of germination and a preparation method of the coarse cereal food. The coarse cereal food subjected to treatment of germination consists of the following coarse cereal materials in parts by weight: 100g of millet, 100g of glutinous broom corn, 100g of sorghum, 100g of black soybean, 50g of grain amaranth, 100g of buckwheat, 100g of oat, 100g of black rice, 150g of coix seed, 20g of mung bean, 10g of pea, 20g of red bean, 5g of cowpea and 5g of small lentil. The coarse cereal materials subjected to treatment of germination are purely natural materials and free of toxic and side effects; and the nutritions contained in the materials are activated and easy to be absorbed by the human body and can be used for promoting gastrointestinal peristalsis, helping digestion, increasing appetite and preventing gastrointestinal diseases.

The invention relates to a sustained-release oral ulcer film having biological adhesion and a preparation method of the sustained-release oral ulcer film. According to the formula, the sustained-release oral ulcer film having the biological adhesion consists of the following components in percentage by weight: 5-50% of a bio-adhesion material, 5-80% of a sustained-release film forming material, 0.5-10% of a medicine, 1-50% of a supporting agent, 1-20% of a plasticizer, 0.1-0.5% of a bacteriostatic agent and 0.5-1% of a flavoring agent. The film provided by the invention, which is prepared in a specific production mode, is good in stability, and excellent biological adhesion and sustained-release performance of the film can be kept for a long time; a soluble sustained-release gel can be gradually formed as the film is adhered to an ulcer surface and absorbs water, so that contact of the ulcer surface with external side is prevented; the oral ulcer film provided by the invention has the characteristics of diminishing inflammation, alleviating pain, achieving an antibacterial effect and being free from toxins, irritation, mutagenic action and the like; and the oral ulcer film can promote growth of blood vessel endothelium and proliferation of fibroblasts and keratinocytes, and subsequently, the oral ulcer film can promote regeneration, repair of healing of the ulcer surface.

The invention discloses fermented coarse cereal food and a preparation method of the fermented coarse cereal food. The fermented food is composed of the following coarse cereal materials in parts by weight: 100g of sorghum, 100g of millet, 100g of buckwheat, 100g of oat, 100g of barley, 100g of black rice, 100g of proso millet, 150g of coix seed, 20g of green bean, 20g of phaseolus angularis, 10g of broad bean, 10g of pea, 5g of cowpea, 100g of black bean and 13g of zymophyte. The coarse cereals adopted by the preparation method provided by the invention are completely pure natural materials, thereby being free from tonic or by effect, lactic acid bacteria is used for guaranteeing delicious and soft taste for the fermented food, and the fermented food can promote gastrointestinal peristalsis, improve digestive ability, add appetite and prevent diseases.

The invention relates to the technical field of biological engineering and the fields of food and medicaments, particularly provides a method for extracting refined yeast grease from yeast, particularly waste saccharomyces cerevisiae and oil production yeast, and further provides soft capsules prepared by compounding the yeast grease, vegetable grease rich in necessary fatty acid for a human bodyand anti-oxidation grease and a preparation method thereof. The obtained yeast grease composite soft capsules have the effects of reducing serum cholesterol and reducing blood fat; the method for producing the yeast grease by using the waste yeast has powerful results of improving the additional value of the waste yeast and generating higher economic benefit; and meanwhile, by comprehensive utilization of the waste yeast, the problem that wastes pollutes environment is solved, and the requirement of circular economy development is met.

The invention relates to a bioreactor for three-dimensional tissue cell perfusion culture. A reaction chamber, a liquid storage tank and a peristaltic pump are sequentially connected through transfusion pipes, wherein the reaction chamber comprises a box cover and a box body comprising lateral walls, a bottom wall, a cut-off device, an overflow insert plate, screen frames, a static pressure channel, a sluice channel and a draining channel; the cut-off device is a gate or a weir flow dam, and a plurality of uniformly arranged water holes are formed in the same plane along the longitudinal section of the cut-off device; an overflowing opening matched with the draining channel is formed in the lateral wall of the static pressure channel; and the screen frames are fixed on two lateral walls of the box body. The invention also relates to a bioreactor with a laminated structure, which can be used for solving the problem of cell death easily caused by non-uniform flow field and non-uniform nutritional transfer in the existing perfusion culture, is suitable for three-dimensional culture of tissue cells, can be used for improving the quality of tissue engineering products, and has the characteristics of good expansibility, strong universality and wide application range.

The invention discloses health-care food for increasing bone density and a preparation method thereof. The health-care food consists of the following components in percentage by weight: 30 to 35 percent of calcium carbonate, 15 to 20 percent of chondroitin sulfate, 20 to 25 percent of glucosamine sulfate, 10 to 15 percent of collagen, 0.05 to 0.1 percent of vitamin D3, 2.5 to 5 percent of sodium hyaluronate, 8 to 10 percent of starch and 1 to 2 percent of magnesium stearate. The health-care food is prepared by crushing the components, sieving and mixing. The health-care food is scientific in formula and compatibility, incompatibility is avoided, the intake of calcium can be increased, the deposition of bone mineral substances in skeletons can also be promoted, the fixing effect of bone tissues on the calcium is improved, the absorption of trace elements of the calcium, phosphorus and the like is promoted, and calcium loss is avoided; and the health-care food can achieve the effect of increasing the bone density when eaten for a long time, is safe and does not have side effects.

The present invention discloses a coronary artery medicine coating scaffold for inhibiting endometrial hyperplasia and preventing restenosis in the scaffold. It includes dilating scaffold base body and inner coating layer coated on the dilating scaffold base body, the above-mentioned inner coating layer is formed from medicine carrier and ligustrazine, their mass ratio is 3:1-1:3, the above-mentioned inner coating layer is one of methyl acrylate, methyl methacrylate copolymer, polylactic acid, polyglycollic acid, polymonoracemic lactic acid, polylactide-diglycolide polymer, phosphorylcholine, ethyl polyurethane inorganic micropore aluminium oxide and cellulose or their composite.

The present invention discloses a fruit and coarse cereal mixed nutrition granule and a preparation method thereof. The fruit and coarse cereal mixed nutrition granule comprises the following raw materials in parts by weight: 22-26 parts of buckwheat, 20-22 parts of red beans, 17-19 parts white kidney beans, 6-8 parts of Diospyros oldhami, 11-13 parts of Rosa roxbunghii, 10-12 parts of Physalis peruviana, 9-11 parts of Holboellia latifolia, 6-8 parts of Rubus buergeri, 8-10 parts of Ribes reclinatum, 6-8 parts of Tremella aurantialba, 10-12 parts of royal jelly, 1-3 parts of camellia pollen, 2-4 parts of lotus pollen, 5-7 parts of seed coat-retained lotus seeds, 5-7 parts of poria, 1-3 parts of radix angelicae, 5-7 parts of grape seeds, 2-4 parts of cucumber seeds and 20-22 parts of crystal sugar,. The fruit and coarse cereal mixed nutrition granule is rich in nutrition and has effects in stabilizing blood pressure, lowering blood lipid and improving human immunity.

The invention provides a preparation method of poly-l-lactide particles and a preparation method of an injectable soft tissue filler. The preparation method of the poly-l-lactide particles includes the steps: first, dissolving poly-l-lactide in good solvents to obtain poly-l-lactide solution; second, adding the poly-l-lactide solution into a reactor and adding anti-solvents under the condition ofstirring to separate out the poly-l-lactide particles from reaction solution; third, filtering the reaction solution with suspending poly-l-lactide particles, purifying and drying the reaction solution to obtain the poly-l-lactide particles. The poly-l-lactide particles are prepared by a soap-free good solvent-anti-solvent precipitation method, addition of any emulsifiers and dispersing agents isomitted in the preparation process of the particles, a technological process is simple in operation, and the preparation method is low in production cost, high in production efficiency and suitable for industrial amplified production.

The invention discloses a rapid fruit tree wound healing protective agent. The rapid fruit tree wound healing protective agent is prepared from the following raw materials in parts by weight: 25-35 parts of vinyl acetate-ethylene copolymerization emulsion, 3-5 parts of hydroxypropylcellulose, 2-4 parts of sodium benzoate, 0.8-1.2 parts of fatty alcohol polyoxyethylene ether, 7-9 parts of ethylene glycol, 20-30 parts of a film forming agent, 5-10 parts of a nutritional agent, 0.2-0.4 part of a growth regulator, 3-5 parts of a chemical bactericide, 6-8 parts of a botanical fungicide, 5-9 parts of an insecticide and 10-20 parts of water. The rapid fruit tree wound healing protective agent disclosed by the invention has the advantages of strong capability of resisting to diseases and pests, outstanding effect, high security and rain wash resistance and can effectively protect a wound after being smeared on a tree body wound caused by pruning, sawing and girdling as well as diseases, prevent infection of germs, reduce water evaporation and stimulate fission of tree body cells to form calluses, a new bark is grown rapidly, the wound is promoted to be rapidly healed, and the rapid fruit tree wound healing protective agent has the special effect on protection of scars caused by applet tree canker, canker and gummosis as well as the wound and a sawing bite.

The invention relates to compound emulsifiable paste for treating acne and a preparation method thereof, in particular to a medicament for treating acne and a preparation method thereof. The compound emulsifiable paste solves the problems of long treatment course and skin oxidation, dim skin and formation of color spots in the healing process in the conventional medicament for treating the acne. The compound emulsifiable paste is prepared from clindamycin hydrochloride, metronidazole, vitamin A, vitamin C, an emulsifying agent, stearic acid, albolene, glycerol monostearate, lanolinum, glycerol and purifying water. The preparation method comprises the following steps of: adding the vitamin A into uniform solution prepared from the stearic acid, the albolene, the glycerol monostearate and the lanolinum to obtain an oil phase; dissolving the glycerol and the emulsifying agent into the purifying water, adding the clindamycin hydrochloride, the metronidazole and the vitamin C, and stirring uniformly to obtain a aqueous phase; and adding the oil phase into the aqueous phase, stirring, homogenizing and standing the mixture to obtain the compound emulsifiable paste for treating the acne. The treatment period of the compound emulsifiable paste is between 14 and 24 days, skin is bright and clean after healing, and the compound emulsifiable paste can treat the acne, seborrheic dermatitis, acne rosacea and folliculitis.

The invention provides a Chinese medicinal preparation for treating virus B hepatitis. The preparation is prepared from the following bulk pharmaceuticals in parts by weight: 10-20 parts of chickenbone herb, 10-20 parts of spreading hedyotis herb, 10-20 parts of fortune eupatorium herb, 10-20 parts of fructus gardenia, 10-20 parts of Chinese thorowax root, 10-20 parts of virgate wormwood herb, 10-20 parts of oriental waterplantain rhizome, 10-20 parts of weeping forsythia, 10-20 parts of isatis root, 10-20 parts of Indian buead, 10-20 parts of common selfheal fruit-spike, 10-20 parts of Chinese magnoliavine fruit, 10-20 parts of immature bitter orange, 10-20 parts of large-head atractylodes rhizome, 10-20 parts of dogwood, 10-20 parts of glossy privet fruit, 10-20 parts of white paeony root, 10-20 parts of baikal skullcap root, 10-20 parts of turmeric root-tuber, 10-20 parts of purple perilla, 10-20 parts of szechwan Chinaberry fruit, 10-20 parts of curcuma zedoary and 10-20 parts of large-leaved gentian. The Chinese medicinal preparation has the effects of soothing the liver, regulating the qi, dispelling the stagnation, promoting blood circulation to dissipate stasis, benefiting qi, reinforcing deficiency, supporting the healthy energy, tonifying liver and kidney, clearing away heat and toxic materials, inducing diuresis, detoxifying, clearing away fire of liver, gallbladder and stomach, protecting liver, lowering transaminase, removing toxins and restoring the body immunity function to normal.

The present invention relates to a compound preparation for effectively curing epithelial erosion. Said compound preparation includes 0.05-15% of aloe yellow juice dried product, immunological competent substance, bactericidal / bacteriostatic agent and biological adhesive or mucosal adhesive carrier, in which the described immunological competent substance is freeze-dried aloe gel product or aloe gel spray-dried product, the described bactericidal / bacteriostatic agent is one kind of chlorhexidine digluconate, chlorhexidine acetate and new benzalkonium chloride or several kinds of them, and the described biological adhesive or mucosal adhesive carrier includes one of hydroxypropyl methyl cellulose, carbomer, alginate, pectine, xylose polyglucosan and polycarbophil or several kinds of them. Said invention also provides its correspondent preparation method, and said compound preparation can be used for effectively curing the diseases of female genital viral and bacterial infection and cervical erosion, etc.

The invention discloses an application of an umbilical cord mesenchymal stem cell medicine in promoting skin wound healing and belongs to the technical field of biological medicines. Umbilical cord mesenchymal stem cells and human fibroblasts induced and differentiated by the umbilical cord mesenchymal stem cells are taken as medicine bodies, the target ability of the medicine is improved throughbonding of small molecular active peptides and medicine-carrying liposome and addition of magnetic particles to the liposome, besides, cells can be supplemented with magnetism, cell activity can be improved, aging can be delayed, and epidermal cell growth is facilitated. An injection is magnetized before injection, the magnetic susceptibility of the injection is increased through a compound electrolyte, the magnetized injection can activate cells in the medicine and cells in the human body, and the medicine effect and the self-healing capacity can be improved. In short, the medicine is applicable to chronic wounds which cannot heal easily and free of toxicity and risks.

The invention provides a pharmaceutical composition for treating thrombocytopenia, which comprises gen-seng, epimeddium, radix astragali, angelica sinensis, radix rehmanniae preparata, spatholobus stems, hairyvein agrimony, and tangerine peel. The pharmaceutical composition of the invention can be prepared into clinically-acceptable dosage forms by adding routine auxiliary materials according to routine technology. The pharmaceutical composition of the invention has significant effect on treating thrombocytopenia and leucopenia, promoting proliferation of bone marrow hematopoietic stem cells, repairing damage and injury of bone marrow hematopoietic tissue caused by radiation, preventing marrow inhibition caused by radiotherapy and chemotherapy, or improving symptoms and increasing life quality of tumor patients, and has no side effect.

The invention provides a nano molecule drug-carrying compound, which is formed by encapsulating a DNA tetrahedron and wogonin according to a molar ratio of 1: (40-200). The invention also provides a preparation method of the compound. The invention also provides the application of the compound in a medicament for the treatment of osteoarthritis. Compared with the single use of DNA tetrahedron andwogonin, the compound has better effect in the aspects of inhibiting inflammatory reaction and improving bone injury, and the compound has good industrialization prospect when being used for preparingmedicaments for treating osteoarthritis.

The invention relates to the field of plant cultivation, and discloses a cultivation method of a peony. The cultivation method comprises the following steps: truncating a peony plant to obtain a stock, selecting a bud, grafting the bud to the stock, healing a grafted part with a healing agent, conveying a first nutritional solution to the peony grafted part with a cotton thread after branch leaves of the peony are rotted to increase the grafting survival rate, then performing covering and conservation measures on the grafted plant, preparing a second nutritional solution from liquid in which potato dregs, plant ash and egg shell powder are soaked, watering the peony with a limited automatic watering device through a liquid guide pipe to enable nutrition to be absorbed in a more balanced, and cultivating a potted peony. Compared with the prior art, the cultivation method of the peony is higher in peony transplanting survival rate and higher in grafting survival rate.

The invention relates to a preparation method of an allogeneic biological patch, and belongs to the technical field of biological material preparation. The preparation method comprises the steps of 1,obtaining people's skin tissues and a corium layer; 2, placing the corium layer in a fixing solution to perform fixing treatment; 3, placing the fixed corium layer in a decellularization solution toperform decellularization treatment to obtain decellularization corium tissues. The fixing solution is a phosphate buffer solution containing 1%-2% formaldehyde and 1.5%-3.0% o-phthalaldehyde; the decellularization solution is a 1.5-2.0 mol / L NaO or KOH alkaline solution containing 10-500 U / L trypsin, 1-25 mmol / L calcium chloride and 1-25 mmol / L magnesium chloride. The allogeneic biological patchhas effective fixing and supporting effects on breast implants, and different sizes are designed to satisfy the requirement of different patients and operating methods; in addition, because extremelylow immunogenicity and extremely excellent biocompatibility of the allogeneic biological patch, it is expected that occurrence rate of diolame contracture after the breast implants are implanted can be significantly reduced.

An orally taken target medicine for treating the ulcerative colitis with high curative effect and low dosage is prepared from the extract of American periplaneta. Its preparing process is also disclosed.

The invention provides a special makeup remover for minimizing pores and moistening skin. The makeup remover is prepared from raw materials of components in percentage by weight as follows: 5%-20% of a surfactant, 5%-20% of a moisturizer, 2%-5% of salicylic acid, 0.5%-2% of a pot marigold extract, 0.5%- 1% of a seaweed extract, 1%-3% of a tea extract, 1%-3% of pentapeptide, 0.5%-2% of vitamin A, 0.01%-0.02% of methylisothiazolinone, 0.2%-1% of a coenzyme Q10, 0.1%-0.5% of sodium citrate and the balance of deionized water. The makeup remover is prepared from four components including salicylic acid, the pot marigold extract, the seaweed extract and the tea extract which have effects of shrinking and minimizing the pores, the four components are matched for use and have the more excellent effect than one single component, the pore minimizing effect is very significant, and besides, the makeup remover removes makeup thoroughly, is free of residues, irritation and oily feeling and can effectively prevent the skin from ageing, moisten and tender the skin and smooth the pores. Meanwhile, the invention further provides a preparation method of the makeup remover.

The invention belongs to the technical field of wash supplies, and relates to a pure nature moisturizing and acne removal handmade soap and a preparation method and application thereof. The handmade soap is prepared from the following raw materials in percentage by weight: 20-30 parts of butyrospermum parkii butter and / or sweet almond oil and / or macadamia nut seed oil, 41-45 parts of olive oil and / or grape seed oil and / or sesame oil, 20-30 parts of coconut oil and / or avocado oil and / or jojoba oil, 1.5-3 parts of tea tree essential oil and / or lavender essential oil and / or ravensare essential oil, 0.5-1 part of white willow bark essence and / or natural salicylic acid, and 0.1-0.5 part of encalyptus globulus leaf oil and / or melaleuca viridiflora essential oil and / or angelica essential oil. Thepure natural moisturizing and acne removal handmade soap can better achieve the effect of moisturizing and acne removing of human body through washing and protection. The preparation method of the pure natural moisturizing and acne removal handmade soap can better prepare the pure natural moisturizing and acne removal handmade soap under the situations that natural plant raw materials are completely adopted, no chemical ingredients and preservatives is added, the soap is pure naturally aired, no waste materials and waste gas is generated and no pollution is emitted.

The invention provides a preparation method for multifunctional hydrogel used for 3D printing.Raw herbal materials of radix angelica sinensis and radix astragali are mixed and smashed, and then a water-extraction and alcohol-precipitation method is used for extracting radix angelica sinensis and radix astragali polysaccharides; radix angelica sinensis and radix astragali polysaccharide powder is added into a dry reaction kettle, then ethyl alcohol is added, NaIO4 with the mass 0.4-0.6 time that of the radix angelica sinensis and radix astragali polysaccharides is dissolved in 200 ml of water, and the solution is added into a reaction system for reacting for 8 h in a lucifugal state; glycol is added, absolute ethyl alcohol with the volume about 2 times that of the reaction system is added into the reaction system, stirring is carried out for 10 min before suction filtration, then a dialysis bag with the molecular cut-off of 3,500 is used for dialysis for 72 h, drying is carried out for 12 hours at the temperature of 50 DEG C, and oxidized radix angelica sinensis and radix astragali polysaccharides are obtained; the oxidized radix angelica sinensis and radix astragali polysaccharides are weighed and dissolved in distilled water according to the mass-to-volume ratio of 1:20, N,O-carboxymethyl chitosan with the mass 1-3 times that of the oxidized radix angelica sinensis and radix astragali polysaccharides is weighed and dissolved in distilled water according to the mass-to-volume ratio of 1:10, the two solutions are mixed in an isovolumetric mode, standing is carried out for 10 min at room temperature, and the multifunctional radix angelica sinensis and radix astragali polysaccharide gel is obtained.

The invention discloses a plant bud grafting wound healing agent. The plant bud grafting wound healing agent is prepared from, by weight, 6-8 parts of polyvinyl alcohol, 10-16 parts of water, 3-5 parts of sodium humate, 2-4 parts of nutrient element additive, 0.01-0.05 part of chitooligo saccharide, 0.5-0.9 part of iron vitriol, 1-2 parts of bactericide, 12-14 parts of a protective agent, 2-3 parts of a tackifier, 2-4 parts of nutrient and 0.1-0.3 part of auxin. The healing agent can effectively avoid bud grafting incisional wound infection and rotting, bacterium infection is avoided, water evaporation is reduced, wound healing is promoted, the bud grafting success rate is increased, the healing agent integrates sterilization, nourishment and moisturizing, use is flexible and convenient, the effect is outstanding, the safety is high, and rain wash resistance is good; meanwhile the preparation method is low in material cost, raw materials are easy to obtain, the technology is simple, and the high practical value and the good application prospect are achieved.

The preparation process of high content immune globulin whey powder includes the following steps: improving the heat stability of cow colosrum, defatting, precipitating casein, isocratic elution for ultrafiltering and concentrating immune globulin from whey, measuring the separation degree of the immune globulin, vacuum freeze drying immune globulin to obtain milk powder, and packing. Test shows that the high content immune globulin whey powder has effective immune globulin content over 50 % and can raise immunity and prevent diseases effectively.

The invention discloses a big tree transplanting method. Aiming at some aspects of tree species selection, tree body selection, soil treatment before excavation, soil ball treatment and plantation treatment, a big tree transplanting processing method is systematically provided to promote the increase of absorbing roots of trees before transplantation, and therefore the survival rate and the seedling growth quality of big trees after transplantation are improved, appropriate transplantation time and tree species with a high survival rate are prerequisites to big tree survival, the soil treatment before excavation gives moisture and nutrient to the roots of trees to be excavated, damage to the roots during the excavation process is reduced, the quality of the roots when the trees are transplanted and excavated is guaranteed, by employing a correct soil ball protection and transport method, damage to soil balls is reduced, by employing a correct plantation method and nutrition supply of filled earth, nutrient and growth of the big trees after transplantation are guaranteed, and therefore the survival rate and the seedling quality of the big trees after transplantation are effectively improved.

The invention relates to a traditional Chinese medicine compound for treating diabetes mellitus and a complication thereof as well as a preparation method thereof. The traditional Chinese medicine compound comprises the following compositions: euonymus alatus, coptis chinensis, pseudo-ginseng and trichosanthin or comprises effective parts thereof. The traditional Chinese medicine compound has the actions of controlling diabetic peripheral neuropathy and retinopathy, promoting beta cell hyperplasia, lowering glycosylated hemoglobin and the like.

The invention provides a method for preparing an integration bone cartilage stent based on a 3DP and laser cladding compound process. The 3DP technology is adopted for preparing a stent cartilage lower bone layer, the laser cladding technology is adopted for preparing a cartilage layer, the 3DP and laser cladding technology is adopted for preparing a stent calcification layer, the requirement forthe biocompatibility of the bone cartilage is met while precise preparation of the integration bone cartilage stent is achieved, the requirement for the connecting strength and mechanical strength among all layers of tissue of the stent is met, and the integration bone cartilage stent which meets the structural gradient and mechanical gradient bionics is prepared.

The invention relates to a chitosan / calcium alginate needleless electrospinning nanofiber membrane for medical dressings and a preparation method thereof, and belongs to the field of natural biological polymer materials. According to the method, chitosan and a PEO (or PVP) solution and a sodium alginate solution and a PEO (or PVP) solution are placed on the two sides of an electrospinning solutionbox respectively, the two kinds of the solutions are gathered at spinnerets, polyelectrolyte self-crosslinking of the sodium alginate and the chitosan is allowed to be conducted in the volatilizationprocess of an electrospinning solvent to obtain a polyelectrolyte electrospinning nanofiber composite membrane composed of the self-crosslinking chitosan / sodium alginate, crosslinking is conducted onthe nano composite membrane by adopting a calcium chloride crosslinking method, so that calcium alginate is generated by the replacement of the sodium alginate and divalent calcium ions, and therefore the calcium alginate-chitosan composite nanofiber membrane is obtained. By means of the method, the mechanical performance and using performance of the material are improved through electrostatic force and calcium chloride crosslinking synergistic reaction, and the biological compatibility of the chitosan and the calcium alginate is fully achieved.