110 results about "Wogonin" patented technology

The invention discloses a new application of a chemical component of chemical component used as plant estrogen, in particular relates to an application of an eucommia bark extract in preparing a medicine for treating and / or preventing a disease relevant to the estrogen hyposecretion of a mammal (particularly a human). The invention relates to an application of a composition in preparing a medicine for treating and / or preventing a disease relevant to the estrogen hyposecretion of a mammal (particularly a human), which is selected from one or more of the following components: betulinic acid, genipin, aucubin, pinoresinol diglucoside, syringaresinol diglucoside, abietinol monoglucoside, syringaresinol monoglucoside, wogonin, oroxylin A, baicalein and dihydrochalcone 3-O-beta-D-glucoside and a medicine composition containing the eucommia bark extract or an active component in the eucommia bark extract. The eucommia bark extract and a monomer chemical component in the eucommia bark extracthave the effect of effective plant estrogen.

The invention relates to a pharmaceutical composition for inhibiting cancer stem cell growth or cancer cell metastasis by using wogonin, and an application thereof. The pharmaceutical composition comprises a wogonin compound and a pharmaceutically acceptable vector. The application of the pharmaceutical composition is to prepare medicaments for inhibiting the cancer stem cell growth or the cancer cell metastasis by using the wogonin compound.

The invention relates to a method of preparing, separating and purifying baicalein and wogonin by endogenous enzymatic hydrolysis of baicalin and wogonoside in scutellaria, and aims at providing a simple, safe, economical and high-efficient method of extracting, separating and purifying the high-purity baicalein and wogonin. An adopted technical scheme is that the traditional Chinese medicine scutellaria is used as a raw material, a unique endogenous enzyme induction bioconversion technology, a negative-pressure cavitation extraction technology, a liquid-liquid extraction technique, a normal-phase silica-gel medium-and-low-pressure preparation liquid chromatography technology, a low-temperature crystallization and recrystallization technology and other high-efficient conversion, extraction, separation and purification technologies are used, and finally the high-purity baicalein and wogonin are obtained, wherein conversion rates of the baicalin and the wogonoside can respectively reach 98.39 % and 98.16 %, contents of the target products: baicalein and wogonin are increased by 4.47 times and 2.85 times, and a purity can be more than 98 %. The method overcomes disadvantages of large damaging effects on the baicalin and the wogonoside, low conversion rate and serious pollution existing in prior art, has advantages of simple production process, safe and environmental protection, high conversion rate and high target compound purity, and has a very important significance for development of medicines and health care products, and industrial production and application.

The invention relates to a novel purpose of eucommia chemical components as blood vessel protective agents. Specifically, the invention relates to a purpose of eucommia or eucommia extracts in preparing medicines used as blood vessel protective agents and / or antihypertensive agents, or in preparing medicines used for treating and / or preventing vascular proliferation diseases. The eucommia extracts comprise at least one component selected from the following: geniposide, wogonin, Qianye extract A, alpha-oxygen-beta-D-glucopyranoside-4,2', 4'-trihydroxyl dihydrochalcone, and betulic acid. According to the invention, the 5 chemical components or the eucommia extracts comprising the 5 chemical components can be used as effective blood vessel protective agents and / or antihypertensive agents, and can be used for treating and / or preventing vascular proliferation diseases.

An anti-inflammatory tablet HPLC fingerprint construction method comprises the following steps: 1, preparing a test solution: grinding an anti-inflammatory tablet, weighing the ground tablet, placing the ground tablet in an extractor, adding petroleum ether, carrying out hot reflux extraction, removing the petroleum ether, adding methanol, carrying out ultrasonic treatment, supplementing methanol, and filtering the obtained solution; 2, preparing a reference solution: taking a chlorogenic acid reference substance, an aesculetin reference substance, a scutelloside reference substance, a linarin reference substance, a baicalein reference substance and a wogonin reference substance; and 3, determining: respectively taking the reference solution and the test solution, respectively injecting the reference solution and the test solution to a liquid chromatograph, recording the chromatogram in 120min, and processing the chromatogram through using fingerprint software to obtain the fingerprint of the anti-inflammatory tablet. The method has the advantages of establishing the common mode of the HPLC characteristic fingerprint of the anti-inflammatory tablet, calibration of 27 common peaks, effective characterization of the quality of the anti-inflammatory tablet, overcoming of unicity and one-sidedness of original quality control methods, and high application values.

The invention discloses a Lanqin oral solution fingerprint spectrum establishment method, a fingerprint spectrum of a Lanqin oral solution and application of the fingerprint spectrum. A test solutionis prepared from the Lanqin oral solution, a reference substance solution is a solution which dissolves adenosine, epigoitrin, phellodendrine chloride, chlorogenic acid, magnoflorine, geniposide, benzoic acid, berberine hydrochloride, baicalin, wogonoside, baicalein and wogonin, and the liquid chromatography is measured by using a high-performance liquid chromatograph; the obtained liquid chromatography is introduced into a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system for analysis, and the Lanqin oral solution fingerprint spectrum is obtained after multi-point correction, data matching and analysis. According to the Lanqin oral solution fingerprint spectrum establishment method and the fingerprint spectrum of the Lanqin oral solution, the quality information of the Lanqin oral solution can be comprehensively reflected, and uniform and stable product quality is ensured.

The present invention relates to application of scutellaria total flavone extract and baicalin in preparation of medicine for curing and / or preventing osteoporosis. The baicalin is 5,6-dihydroxyflavone-7-0-glucoglycuronide, and the scutellaria total flavone extract includes baicalin, baicalein, wogonin, wogonoside and neobaicalein, etc. as its main effective components.

The invention relates to a new usage of eucommia ulmoides chemical composition as vessel protective agent, in particular to the new application of the eucommia ulmoides or eucommia ulmoides extractive for preparing the medicines as vessel protective agent and / or antihypertensions, or the medicines for curing and / or preventing angiogenesis disease. The eucommia ulmoides extractive contains at least one component selected from the group consisting of geniposide, wogonin, chiba A, alpha-oxygen-belta-D-glucopyranosyl-4,2',4'-trihydroxy dihydrochalcone and betulinic acid. According to the invention, the five chemical components or the eucommia ulmoides extractive containing the chemical components can be used as effective vessel protective agent and / or antihypertensions for curing and / or preventing angiogenesis disease.

The invention provides a method for measuring the contents of baicalin, baicalein and wogonin in cold cough syrup.The method for measuring the contents of baicalin, baicalein and wogonin in cold cough syrup aims at being accurate in measurement and easy to implement.The method comprises the steps that firstly, a standard solution is prepared; secondly, a test solution is prepared, wherein l ml of the test solution is measured and put in a 20 ml volumetric flask and dissolved with 50% methyl alcohol, ultrasonic treatment is carried out for 5 min, 50% of the solution is used for reaching the constant volume of the 20 ml scale mark, even mixing is carried out, 1 mL of the solution is precisely measured and passes through a C18-SPE column at the flow speed around 1 mL / min, elution is carried out with 2 mL of 50% methyl alcohol, the flowing-out solution and the elution solution are mixed and filtered with a 0.22-micrometer organic membrane, and a sample to be detected is obtained and put into a high performance liquid chromatograph for detection.The method belongs to the technical field of chemical detection.

The invention relates to genetically engineered saccharomycetes for producing baicalein compounds as well as a construction method and application of the genetically engineered saccharomycetes. A series of exogenous genes are introduced into yeast engineering bacteria through a gene recombination technology to obtain the yeast engineering bacteria capable of producing baicalein, baicalin or wogonin. The yeast engineering bacteria have the characteristics of a low metabolic background, strong heterologous expression ability, no need of additional addition of a precursor, capability of synthesizing a final product in a whole-cell manner, easiness in separation of the final product and the like, and a novel thought is provided for industrial production of flavonoid drugs.