Method for simultaneously separating wogonin and baicalein monomers from scutellaria baicalensis

Abstract

The invention provides a method for simultaneously separating wogonin and baicalein monomers from scutellaria baicalensis. The method comprises the steps of crushing a scutellaria baicalensis medicinal material, adding a proper amount of water and edible enzyme to carry out enzymolysis, supercritically extracting the medicinal material subjected to the enzymolysis, fractionally collecting the medicinal material by using an entrainer, and re-crystallizing to respectively obtain baicalein and wogonin. By using the method, baicalein compound monomers can be simultaneously extracted from the scutellaria baicalensis medicinal material, the yield is increased by over 3 times than the yield of wogonin and baicalein monomers obtained by using a direct separation method, and the purities of the prepared wogonin and baicalein monomers are both over 98%; moreover, the method is simple and convenient in flow, environment-friendly, very important in production practical significance, and suitable for large-scale industrial production.

Description

Technical field [0001] The invention relates to the field of separation of natural active substances, in particular to a method for simultaneously separating wogonin and baicalein monomers from scutellaria. Background technique [0002] Scutellaria baicalensis is the dried root of Scutellaria baicalensis Georgi, a plant in the Lamiaceae family. It has the functions of clearing away heat and dampness, purging fire and detoxification, hemostasis, and anti-fetus. The main components of scutellaria are flavonoids such as Baicalin, Wogonside, Oroxylin and a small amount of Chrysin. Drug metabolism studies have found that the main components absorbed into the blood by the extract of Scutellaria baicalensis are baicalein and wogonin and a small amount of lamellarin. It is difficult for baicalin and wogonin to be absorbed by the intestine, and the bioavailability is extremely low. In recent years, it has been confirmed that wogonin has a clear anti-tumor effect. For example, wogonin can...

AI Technical Summary

Problems solved by technology

Due to the low content of baicalein and wogonin in plants (generally 0.05-0.5%), it is difficult to separate directly; acid hydrolysis method can convert part of baicalin into its aglycone, but because flavonoid glycosides are 7- 0-Glucuronic acid-binding glycoside, the glycosidic bond is relatively strong and difficult to break, so the transfer rate of acid hydrolysis is limited to a certain extent; the degradation conditions of endogenous enzyme method are relatively mild, but it takes a long time, and silica gel column and a large amount of organic solvent are still needed after degradation Baicalein and wogonin can only be separated and obtained. Therefore, the existing preparation methods have certain limitations in terms of transfer rate, operability, safety, cost, etc.

In addition, baicalein and wogonin have o-triphenolic hydroxyl groups in their structures, and their properties are unstable. They are easily oxidized into quinone structures and denatured, which increases the difficulty of separation and preparation of these compounds.

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