111 results about "Isoliquiritigenin" patented technology

The invention discloses a method used for separating and identifying flavonoid matters in tobacco by adopting liquid chromatography-mass spectrography. The method comprises the following steps: a sample is ground and dried, an extractant is added according to the standard that 0.1 ml of the extractant is added into 1 mg of the sample for extraction and centrifugation, a supernatant liquor is collected, water and chloroform are added according to the standard that 0.5 ml of the water and 1 ml of the chloroform are added into 1 ml of the supernatant liquor for centrifugation, and the supernatant liquor is collected for analysis; 153+ and 151- are adopted as characteristic ions for scanning, parent ions are extracted for second level mass spectrum scanning, and an obtained spectrogram is compared with a spectral library to determine a chemical structure and verified through a reference substance; the molecules and ions of the identified matters and myricetin, dihydromyricetin, catechinic acid / epicatechin, daidzein, glycitein and glycyrrhizin / isoliquiritigenin are adopted as characteristic ions for scanning, the molecules and ions of the searched glycosylation flavonoid matters are used as the parent ions for the second level mass spectrum scanning, an obtained spectrogram is compared with the spectral library to determine a chemical structure and verified through the reference substance. According to the invention, the method is suitable for the analyzing and detecting micro even trace flavonoid matters.

A sobering-up beverage is proportionally prepared from 8 raw materials including purified water, pear juice, pueraria root (or flower), ginsenoside Rg3, rebescensine A, isoliquiritigenin, etc.

The invention relates to a method for controlling quality of a liquorice medicinal material and a liquorice preparation. The method concretely relates to the step of simultaneously determining the content of the liquorice medicinal material and the content of six main active ingredients, namely liquiritin, isoliquiritin, liquiritigenin, isoliquiritigenin, glycyrrhizic acid and glycyrrhetinic acid in the liquorice preparation by utilizing a high performance liquid chromatography. A new method for controlling the quality of the liquorice and the liquorice preparation is established and the six main active ingredients, namely liquiritin, isoliquiritin, liquiritigenin, isoliquiritigenin, glycyrrhizic acid and glycyrrhetinic acid are quantitatively determined simultaneously by utilizing the high performance liquid chromatography. Confirmed by methodological investigation and test, the method is simple and convenient, correct in result and good in reproducibility, the defect of the existing quality control is overcome, and the quality control on the liquorice and the liquorice preparation is perfect and scientific.

The invention relates to a fusion protein, a gene used for encoding the fusion protein and a recombinant engineering bacterium containing the gene. The fusion protein comprises chalcone synthase and chalcone reductase, and the chalcone synthase is connected with the chalcone reductase through a connexin GGGS. The fusion protein can be applied to biosynthesis of chalcone and isoliquiritigenin.

The invention relates to a method for synthesizing isoliquiritigenin, comprising the following steps: using 4-hydroxy benzaldehyde and 2,4-dihydroxyacetophenone as raw materials; and carrying out hydroxyl protection, aldol condensation and dehydroxylation protective reaction to obtain the isoliquiritigenin. The method is simple, and the raw materials have low cost and high yield and are easy to obtain.