111 results about "Isoliquiritigenin" patented technology

A composition having phytoestrogenic and anti-cancer activity is described. The composition comprises wogonin, isoliquiritigenin, coumestrol, their pharmaceutically acceptable salts or esters, their selectively substituted analogs, or combinations thereof. The compositions may also include an anti-cancer agent and / or an immune stimulant. A method for treating or preventing cancer or an estrogen-related disorder includes administering a therapeutically effective amount of the compositions is described. The compositions are particularly useful in the treatment of hormone-related cancers.

The present invention discloses preparation process of licoflavone from licorice and licorice slag, and features licoflavone is extracted from crushed licorice or licorice slag after extracting glycyrrhetic acid through adding organic solvent for soaking, filtering, and concentrating the filtrate; and that the prepared licoflavone contains licochalcone, isoliquiritigenin, glabidin, liquiritin, liquiritigenin and other components. The preparation process is simple, high in yield and suitable for industrial production.

A synergistic clean extraction method of effective components in licorice root adopts a solvent to synchronously extract the effective components in licorice root in order to obtain a total extract product, and the total extract product is separated one by one according to physical and chemical properties of all the components. The method comprises the following steps: 1, preparing the total extract product of the licorice root; 2, separating water-soluble and fat-soluble components; 3, separating glycyrrhizic acid from licorice pigments, licorice glucose and licorice polysaccharides in the water-soluble components; 4, separating the licorice pigments from the licorice glucose and the licorice polysaccharides; 5, separating the licorice glucose from the licorice polysaccharides; 6, separating and purifying glabridin in the fat-soluble components; 7, separating and purifying liquiritin and isoliquiritin in the fat-soluble components; 8, separating and purifying isoliquiritigenin and liquiritigenin in the fat-soluble components; 9, separating and purifying licochalcone A in the fat-soluble components; 10, separating and purifying licorice lignin in the fat-soluble components; and 11, separating and purifying licorice cellulose in the fat-soluble components.

A composition having phytoestrogenic and anti-cancer activity is described. The composition comprises wogonin, isoliquiritigenin, coumestrol, their pharmaceutically acceptable salts or esters, their selectively substituted analogs, or combinations thereof. The compositions may also include an anti-cancer agent and / or an immune stimulant. A method for treating or preventing cancer or an estrogen related disorder includes administering a therapeutically effective amount of the compositions is described. The compositions are particularly useful in the treatment of hormone-related cancers.

The invention discloses a method used for separating and identifying flavonoid matters in tobacco by adopting liquid chromatography-mass spectrography. The method comprises the following steps: a sample is ground and dried, an extractant is added according to the standard that 0.1 ml of the extractant is added into 1 mg of the sample for extraction and centrifugation, a supernatant liquor is collected, water and chloroform are added according to the standard that 0.5 ml of the water and 1 ml of the chloroform are added into 1 ml of the supernatant liquor for centrifugation, and the supernatant liquor is collected for analysis; 153+ and 151- are adopted as characteristic ions for scanning, parent ions are extracted for second level mass spectrum scanning, and an obtained spectrogram is compared with a spectral library to determine a chemical structure and verified through a reference substance; the molecules and ions of the identified matters and myricetin, dihydromyricetin, catechinic acid / epicatechin, daidzein, glycitein and glycyrrhizin / isoliquiritigenin are adopted as characteristic ions for scanning, the molecules and ions of the searched glycosylation flavonoid matters are used as the parent ions for the second level mass spectrum scanning, an obtained spectrogram is compared with the spectral library to determine a chemical structure and verified through the reference substance. According to the invention, the method is suitable for the analyzing and detecting micro even trace flavonoid matters.

The invention discloses micro-emulsion composition containing isoliquiritigenin which comprises isoliquiritigenin, emulsifying agent, assistant for emulsifying agent, oil and water. By using the composition, the solubility of the isoliquiritigenin can be greatly improved.

The invention discloses a synthetic method of a chemical substance isoliquiritigenin. The synthetic method comprises the steps as follows: A. 2,4-dihydroxyacetophenone is synthesized under the catalysis of Lewis base; B. 2-hydroxy-4-methoxyacetophenone is synthesized; C. 3-(4-hydroxyphenyl)-1-(4-methoxy-2-hydroxyphenyl)-acrylketone is synthesized and recrystallized; D. 3-(4-hydroxyphenyl)-1-(2,4-dihydroxyphenyl)-acrylketone, that is, the isoliquiritigenin, is synthesized and recrystallized; E. the crude products of the obtained isoliquiritigenin is eluted and refined by column chromatography to obtain the isoliquiritigenin product, and after the detections of ultra violet, infrared, mass spectrum and nuclear magnetic resonance, the isoliquiritigenin product has the same spectrogram with the isoliquiritigenin standard product. The synthetic method of the invention has the advantages of simple method, available raw materials, low price, higher yield, etc.

The invention discloses an extraction and preparation method by comprehensively utilizing liquorice and belongs to the technical field of traditional Chinese medicine. Isoliquiritigenin, glycyrrhizic acid and liquorice polysaccharide which have high additional values are separated and prepared by a polyamide-macroporous resin coupling technique. The extraction and preparation method has the advantages of simple technical route and green and safe production process, does not need to use toxic and organic solvents and is suitable for mass production, the comprehensive utilization degree of the liquorice is greatly improved, the traditional Chinese medicine resource is saved, and good social benefit and economic benefit are generated.

The invention relates to a method for extracting isoliquiritigenin from iquorice. The method uses a property of flavonone which can be transformed into chalcone in the presence of alkali, an acid hydrolysis product of liquidritin is transformed into the isoliquiritigenin, thereby obviously improving the yield of extracting the isoliquiritigenin. With the method, the yield of extracting the isoliquiritigenin is 32 times of that of ethanol ultrasonic extraction, and 1.7 times of that of acid hydrolysis extraction.

The invention discloses an antitumor isoliquiritigenin tablet. The isoliquiritigenin is used as an effective component to prepare antitumor tablet. The antitumor isoliquiritigenin tablet contains isoliquiritigenin, filler, adhesive, disintegrant, wetting agen and lubricant, which are proportioned according to a certain proportion. The formula is reasonable and the use is convenient. The drug combination prepared in the method has good antitumor function, good treating effects and low side effects.

The invention belongs to the field of medicine and specifically relates to novel application of isoliquiritigenin, more specifically to application of isoliquiritigenin in preparation of drugs used for improving and treating psoriasis, drugs used for improving or treating parakeratosis, hyperkeratosis, acanthosis and inflammatory cell infiltration, drugs used for inhibiting the expression levels of NF-kB and p-NF-kB, and drugs used for improving or treating autoimmune diseases.

The invention discloses the use of isoliquiritigenin as a medicament for preventing and treating or curing postoperative metastasis and relapse of malignant tumors, which belongs to technical field ofmedicaments. The invention discloses the use of isoliquiritigenin as a medicament for preventing and treating or curing postoperative metastasis and relapse of malignant tumors. Researches show thatthe surgical resection of primary tumors can promote tumor metastasis. At present, no ideal therapeutic method or medicament for treating malignant tumor metastasis and relapse after the resection ofthe primary tumors is available. Experiments implemented by the inventor prove that the isoliquiritigenin has a remarkable inhibition effect on the relapse and metastasis of various malignant tumors after the surgical resection of the primary tumors. Based on the effect, the isoliquiritigenin can be used in clinic prevention or treatment of the relapse and metastasis of lung cancer, liver cancer,breast cancer, colorectal cancer and other various malignant tumors after the surgical resection of the primary tumors and has the obvious advantages of high safety, low price, good patient complianceand the like. The effect of the isoliquiritigenin may be related to the inhibition of the isoliquiritigenin on the self renewal and multiplication capacity of cancer stem cells.

The invention relates to an extraction and purification method of licoflavone ingredients, and belongs to an extraction and purification method of licoflavone ingredients in plants. Liquorice roots are used as raw materials and are extracted by distilled water to obtain a liquorice root extraction solution; then, through methanol and ethyl acetate treatment, the flavone ingredients in the liquorice roots are coarsely separated; a DM130 macroporous resin and polyamide resin combined column passing method is used; finally, high performance liquid-mass spectrometry is used for performing ingredient identification on the purified flavone ingredients. The method has the beneficial effects that the DM130 macroporous resin and polyamide resin combined method is for better separating and purifyingthe lavone ingredients in the liquorice roots; in addition, the influence of saponin ingredients is reduced to the minimum; the pharmacodynamic study and the like on flavone ingredients in the liquorice roots are facilitated; the licoflavone identified by the liquid chromatography-mass spectrometry is mainly glycyrrhizin glucoside, liquiritin, isolicorice glucoside, isoliquiritin, glycyrrhizin, isoliquiritigenin, formononetin, glicoricone, glycyrrhizic acid and glycycoumarin.

The invention discloses an endophytic bacterial strain Penicillium commune CGMC CNO.6113 and an application in the flavone production. Compared with coordinative bacterial strains, the Penicillium commune CGMC CNO.6113 bacterial strain is apparent in the flavone production in mycology. When the bacterial strain is fermented, main ingredients of glycyrrhiza flavonoids of liquiritin, glycyrrhizin, isoliquiritigenin and the like can be produced, and the bacterial strain can be applied in antioxidation, senescence delaying, inflammation elimination and the like as a prevention and treatment method, accordingly, the endophytic fungi for producing flavones and the application thereof provide a novel approach for the development and application of glycyrrhiza flavonoids and have wide applicationvalues.

The invention belongs to the technical field of medicine eutectic crystal, and concretely discloses an isoliquiritigenin pyrazinamide eutectic crystal and a preparation method thereof. According to the method, isoliquiritigenin and pyrazinamide are used as raw materials; a solvent auxiliary grinding method or a solvent suspension method is used for preparing the isoliquiritigenin pyrazinamide eutectic crystal. The obtained eutectic crystal has a novel crystal form, is favorable for novel preparation development, and promotes the application of the isoliquiritigenin to medicine clinics.

A method for measuring contents of 14 chemical components in a traditional Chinese medicine composition is disclosed. HPLC-MS / MS is adopted to simultaneously measure the contents of the 14 chemical components in the composition. The composition is a pulse-invigorating heart-tonifying pill. The 14 chemical components include emodin, lobetyolin, 2,3,5,4'-tetrahydroxy stilbene-2-O-beta-D-glucoside, glycyrrhizic acid, liquiritin, liquiritigenin, polydatin, rutoside, isoliquiritigenin, ononin, verbascoside, gallic acid, schizandrin, and rhein. The method includes building standard curves of the 14chemical components; acquiring multi-reaction ion chromatograms of the 14 chemical components in the pill; and determining the contents of the 14 chemical components in the pill. The method has characteristics of high sensitivity, high reliability, a high efficiency and capability of being rapid. Through measuring the contents of the 14 chemical components in the pill, quality control on the pillcan be more effectively performed, thus ensuring clinical treatment effects of the pill.

The invention provides Caulis Spatholobi extract, an application thereof and a new application of isoliquiritigenin, and particularly provides Caulis Spatholobi extract containing isoliquiritigenin, an active part and a monomer compound isoliquiritigenin as well as an application of a composition in preparing medicine for treating and preventing triple-negative breast cancer. The composition consists of isoliquiritigenin and one or more active ingredients in gallocatechin, epicatechin and catechin.

A sobering-up beverage is proportionally prepared from 8 raw materials including purified water, pear juice, pueraria root (or flower), ginsenoside Rg3, rebescensine A, isoliquiritigenin, etc.

The invention relates to the technical field of organic synthesis, and particularly discloses a synthesis method of isoliquiritigenin. According to the synthesis method of isoliquiritigenin, 2,4-hydroxyacetophenone and 4-hydroxybenzaldehyde are used as raw materials, and the product namely isoliquiritigenin can be obtained through hydroxyl protection, aldol condensation and hydroxyl deprotection reaction. The synthesis method of isoliquiritigenin, provided by the invention, is simple and easy to implement, mild in reaction conditions, safe, reliable, high in raw material availability, low in price and low in cost, adopts at least one of dimethyl tert-butyl chlorosilane and triisopropyl chlorosilane to protect 4-hydroxyl groups of 4-hydroxybenzaldehyde and 2,4-dihydroxyacetophenone, reducesthe risk of byproduct generation, improves the yield of isoliquiritigenin, and is suitable for industrial production.

The invention discloses application of isoliquiritigenin or isoliquiritoside to preparation of anti-aging cosmetic products with high safety performance. Researches prove that isoliquiritigenin or isoliquiritoside can improve the expression level of the procollagen I alpha 1 chain (Col1A1) in human skin fibroblasts, reduce the expression level of matrix metalloproteinase 1 (MMP1), and promote the expression level of the silent information regulator 1 (Sirt1); therefore, isoliquiritigenin or isoliquiritoside can inhibit human skin fibroblast apoptosis and promote synthesis of collagen in human skin fibroblasts, so as to resist aging. Furthermore, isoliquiritigenin or isoliquiritoside has low toxicity to human skin fibroblasts. Researches show that isoliquiritigenin or isoliquiritoside is of great application value in preparation of anti-aging cosmetic products with high safety performance.

The invention provides an external preparation for preventing or treating breast cancer. The external preparation comprises active ingredients and external-preparation auxiliary materials, and is characterized in that the active ingredients are prepared from caulis-spatholobi extract and / or isoliquiritigenin. The caulis spatholobi extract is aulis-spatholobi water extract and ethanol extract, or is extract prepared in the mode that caulis-spatholobi water extract and ethanol extract are extracted sequentially through petroleum ether, ethyl acetate and normal butanol or one of petroleum ether, ethyl acetate and normal butanol, or is an effective part obtained in the mode that the obtained extract is purified through a macroporous resin column. Experimental investigation shows that the caulis-spatholobi extract and / or the isoliquiritigenin have / has the effects of remarkably inhibiting breast cancer to grow and transfer in vitro and vivo, and have / has the good preventing and treating effects on breast cancer.

The invention belongs to the technical field of medicines, and discloses application of isoliquiritigenin to prepare medicaments of inducing a tumor to be differentiated. Tests prove that when the concentration of the isoliquiritigenin is low, the isoliquiritigenin cannot kill the tumor cells, but has the action of reversing the tumor cells in leukemia, liver cancer, malignant melanoma and the like into the cell with normal functions. Based on the characteristics, the isoliquiritigenin is mixed with other medical auxiliary materials to prepare an oral or injectable preparation; when the oral or the injectable preparation is clinically used for treating various malignant tumors, the preparation has the advantages of confirmed curative effect, no obvious toxic and side effects, and the suitability for long-period and continuous medicine-taking; and the medicament prepared by the isoliquiritigenin is obviously better than the prior medicament clinically used for treating the tumors,.

The invention relates to a fusion protein, a gene used for encoding the fusion protein and a recombinant engineering bacterium containing the gene. The fusion protein comprises chalcone synthase and chalcone reductase, and the chalcone synthase is connected with the chalcone reductase through a connexin GGGS. The fusion protein can be applied to biosynthesis of chalcone and isoliquiritigenin.

The invention provides a new use of isoliquiritigenin and its derivative, and especially provides application of isoliquiritigenin, new isoliquiritigenin, natural plants containing isoliquiritigenin and its derivative or its extract in tumor resistance and synergists of chemotherapeutic drugs or drugs reducing the drug resistance of chemotherapeutic drugs.

The invention discloses a soybean chalcone reductase gene CHR4 and an application. The gene CHR4 is a new chalcone reductase gene in soybean; experiments prove that an expression product of the gene can catalyze the synthesis of isoliquiritigenin; the CHR4 comes from soybean and has an optimizing codon suitable for dicotyledons such as soybean and the like; and a genetic engineering receptor is mainly suitable for the dicotyledons such as soybean, tobacco, cotton and the like, and is also suitable for monocotyledons such as rice, wheat, corn and the like. The gene CHR4 can be transferred into a plant as a target gene and is used for catalyzing the synthesis of a precursor substance isoliquiritigenin required for the daidzein biosynthesis so as to improve the content of daidzein in the plant; and moreover, as a plant defensin, the daidzein can improve the plant disease resistance, has an effect on preventing and treating multiple diseases of human bodies, and also is a good healthcare product.

The invention belongs to the technical field of medicine eutectic crystals, and concretely discloses an isoliquiritigenin nicotinamide eutectic crystal and a preparation method thereof. The above medicinal eutectic crystal preparation method adopts isoliquiritigenin as a medicinal active component and nicotinamide as an eutectic precursor, and adopts a solvent assisted grinding technology or a solvent suspension technology to crystallize, and the obtained eutectic crystal has a brand new crystallization form, and is completely different from isoliquiritigenin and nicotinamide crystals in characteristics.

The invention belongs to the technical field of medicines, and relates to application of isoliquiritigenin. The invention provides the application of the isoliquiritigenin, and experimental results show that the isoliquiritigenin can safely and effectively control neuroinflammatory response, so that the cognitive function, dysmnesia and motor coordination ability of brain tissues are improved, and the isoliquiritigenin can be used for preventing and treating Alzheimer's disease, Parkinson's disease and the like. The isoliquiritigenin is applied to preparation of medicines or health-care products for preventing and treating neuroinflammation, and has good application value and development prospect.

The invention relates to eye drops and a preparation method thereof and specifically provides isoliquiritigenin eye drops using isoliquiritigenin as a raw material and a preparation method thereof. The raw materials of the isoliquiritigenin eye drops have an optimum ratio as below: an isoliquiritigenin concentration of 40 mug / ml, a NaCl concentration of 8 mg / ml, a NaH2PO4.H2O concentration of1.44 mg / ml, a KCl concentration of 0.2 mg / ml, a KH2PO4 concentration of 0.24 mg / ml and a carboxymethylcellulose sodium concentration of 5 mg / ml. The eye drops of the present invention are prepared by a simple method and have substantial control effect on newborn blood vessel and wide application and developing prospect. Researches have proved that the eye drops prepared from isoliquiritigenin can effectively inhibit growth of newborn blood vessel of alkali burned mice, reduce infiltration of cornea inflammation cells and improve cornea oedema. Therefore, isoliquiritigenin has substantial curative effect on controlling cornea newborn blood vessel caused by cornea alkali burn and can effectively inhibit generation and development process of newborn eye blood vessel.

The invention relates to a method for synthesizing isoliquiritigenin, comprising the following steps: using 4-hydroxy benzaldehyde and 2,4-dihydroxyacetophenone as raw materials; and carrying out hydroxyl protection, aldol condensation and dehydroxylation protective reaction to obtain the isoliquiritigenin. The method is simple, and the raw materials have low cost and high yield and are easy to obtain.