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Extraction and purification method of licoflavone ingredients

A technology of licorice flavonoids and a purification method, which is applied to medical preparations containing active ingredients, pharmaceutical formulas, drug combinations, etc., and can solve the problems of low water solubility and few applications

Inactive Publication Date: 2018-07-10
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Flavonoids have low water solubility at low temperature and are easy to be saturated, so crystallization can be used to separate flavonoids at different temperatures and different solvent systems, but this method is rarely used

Method used

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  • Extraction and purification method of licoflavone ingredients
  • Extraction and purification method of licoflavone ingredients
  • Extraction and purification method of licoflavone ingredients

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Proceed as follows:

[0021] (1). Preparation of licorice extract

[0022] Accurately weigh the crude extract of licorice, soak in 9 times the amount of distilled water for 25 minutes, reflux for 0.5 hours, centrifuge to separate the supernatant and medicinal residues, extract the medicinal residues with 9 times the amount of water for 35 minutes, centrifuge, and combine the supernatants twice, Concentrate the supernatant to 0.5 g crude drug amount / mL, add 95% ethanol to make the final concentration of the alcohol precipitation solution 60%, let stand at 4°C for 12 hours, centrifuge at 4000r / min for 8min, and concentrate the supernatant to dry, ready to use;

[0023] (2). Rough separation of flavonoid components in licorice extract

[0024] Add a small amount of methanol to the licorice extract concentrated to dryness until dissolved, slowly add ethyl acetate 10 times the volume of methanol under a magnetic stirrer, let it stand for 9 minutes, transfer the supernatant...

Embodiment 2

[0030] Proceed as follows:

[0031] (1). Preparation of licorice extract

[0032] Accurately weigh the crude extract of licorice, soak in 10 times the amount of distilled water for 30 minutes, reflux for 1 hour, centrifuge to separate the supernatant and medicinal residues, extract the medicinal residues with 10 times the amount of water for 40 minutes, centrifuge, combine the supernatants twice, and Concentrate the supernatant to 0.5g crude drug amount / mL, add 95% ethanol to make the final concentration of the alcohol precipitation solution 60%, let stand at 4°C for 12h, centrifuge at 4000r / min for 10min, and concentrate the supernatant to dryness ,stand-by;

[0033] (2). Rough separation of flavonoid components in licorice extract

[0034] Add a small amount of methanol to the licorice extract concentrated to dryness until dissolved, slowly add ethyl acetate 10 times the volume of methanol under a magnetic stirrer, let it stand for 10 minutes, transfer the supernatant, con...

Embodiment 3

[0040] Proceed as follows:

[0041] (1). Preparation of licorice extract

[0042] Accurately weigh the crude extract of licorice, soak in 11 times the amount of distilled water for 35 minutes, reflux for 1.5 hours, centrifuge to separate the supernatant and medicinal residues, extract the medicinal residues with 11 times the amount of water for 45 minutes, centrifuge, and combine the two supernatants, Concentrate the supernatant to 0.5 g crude drug amount / mL, add 95% ethanol to make the final concentration of the alcohol precipitation solution 60%, let stand at 4°C for 12 hours, centrifuge at 4000r / min for 12min, and concentrate the supernatant to dry, ready to use;

[0043] (2). Rough separation of flavonoid components in licorice extract

[0044] Add a small amount of methanol to the licorice extract concentrated to dryness until dissolved, slowly add ethyl acetate 10 times the volume of methanol under a magnetic stirrer, let it stand for 11 minutes, transfer the supernata...

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Abstract

The invention relates to an extraction and purification method of licoflavone ingredients, and belongs to an extraction and purification method of licoflavone ingredients in plants. Liquorice roots are used as raw materials and are extracted by distilled water to obtain a liquorice root extraction solution; then, through methanol and ethyl acetate treatment, the flavone ingredients in the liquorice roots are coarsely separated; a DM130 macroporous resin and polyamide resin combined column passing method is used; finally, high performance liquid-mass spectrometry is used for performing ingredient identification on the purified flavone ingredients. The method has the beneficial effects that the DM130 macroporous resin and polyamide resin combined method is for better separating and purifyingthe lavone ingredients in the liquorice roots; in addition, the influence of saponin ingredients is reduced to the minimum; the pharmacodynamic study and the like on flavone ingredients in the liquorice roots are facilitated; the licoflavone identified by the liquid chromatography-mass spectrometry is mainly glycyrrhizin glucoside, liquiritin, isolicorice glucoside, isoliquiritin, glycyrrhizin, isoliquiritigenin, formononetin, glicoricone, glycyrrhizic acid and glycycoumarin.

Description

technical field [0001] The invention belongs to a method for extracting and purifying flavonoid components in plants. Background technique [0002] In recent years, studies have found that flavonoids have anti-cancer, anti-cancer, anti-tumor, anti-oxidation, anti-inflammatory, anti-bacterial, anti-cardiovascular disease, lowering blood fat, anti-osteoporosis, analgesic, estrogen-like effects, and prevention of Alzheimer's disease As well as various pharmacological effects such as regulating the immune system. Since the mechanism of action of flavonoids in many herbal medicines is still unclear, people have paid more and more attention to the identification of flavonoids in herbal medicines and the research on related pharmacological effects in recent years. [0003] There are many kinds of flavonoids, which generally refer to a series of compounds formed by connecting two benzene rings (A and B) through three C atoms. The basic skeleton has a C6-C3-C6 pattern. The structure...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/484A61P29/00
CPCA61K36/484A61K2236/331A61K2236/39A61K2236/51A61K2236/53A61K2236/55
Inventor 刘忠英刘媛媛韦梦莹马莹慧
Owner JILIN UNIV
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