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178results about How to "Support growth" patented technology

Enhancement of angiogenesis to grafts using cells engineered to produce growth factors

ActiveUS20050002915A1Great ease of implantationSupport growthBiocidePeptide/protein ingredientsSecreted growth factorAngiogenesis Modulating Agents
The present invention provides methods and compositions of engineered cells for use in the continuous or transient delivery of growth factors and angiogenesis modulating agents, such as vascular endothelial growth factor (VEGF), in conjunction with constructs for replacing or augmenting organ functions. In one aspect of he invention, the genetically engineered cells can be immature cells that are capable of differentiating and assimilating into the target region. The methods of the present invention can be used to enhance vascularization locally at a target site in need of repair, growth, or implantation through the incorporation of autologous cells which have been genetically engineered to secrete a growth factor or angiogenesis modulating agent.
Owner:WAKE FOREST UNIVERSITY

Serum-free mammalian cell culture medium, and uses thereof

The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and / or increasing virus production in cultured cells.
Owner:LIFE TECH CORP +1

Blood pump bearings with separated contact surfaces

Rotary hydrodynamic blood pumps have been used to treat over a thousand patients. The Jarvik 2000 has supported a patient for seven years and uses blood immersed bearings washed by high flow to avoid excessive thrombus formation. This permits the pump to be very simple and small. Nonetheless, the present Jarvik 2000 bearings and all other mechanical blood immersed bearings of the prior art have a supporting structure that predisposes to thrombus adjacent to the bearings. The present invention provides a bearing structure that eliminates this predilection site for thrombus formation, and may provide indefinite thrombus free operation. The rotor of the preferred embodiment includes a tapered hub fabricated of wear resistant material supported by three posts at each end of the rotor, upon which the rotor rotates. Blood washes the unobstructed spaces between the posts to prevent the accumulation of a torus of thrombus that could enlarge excessively.
Owner:JARVIK ROBERT

Cryopreservation of pluripotent stem cells

The present invention relates to methods and compositions for the cryopreservation of pluripotent cells in general and human embryonic stem (ES) cells in particular. The stem cells are grown on a bottom layer of solid support matrix and subsequently covered by a top layer of solid support matrix forming a matrix-cell-matrix composition, to which an effective amount of cryopreservation media is added, prior to freezing. The methods of the invention yield cryopreserved cells that exhibit an increase in cell viability and a decrease in cell differentiation, facilitating storage, shipping and handling of embryonic stem cell stocks and lines for research and therapeutics.
Owner:WISCONSIN ALUMNI RES FOUND

Non-serum culture medium for multiple animal cell large-scale culture

InactiveCN1778902ASupport growthSupports long-term subcultureAnimal cellsHigh densityEssential amino acid
The invention opened non-serum mediums which can proper to culture many kinds of the animal cells. The character of it is to use the DMEM / F12 as the base medium, then add the growth factor, hormone, the essential amino acid and the microelement. It has the functions: (1) it can support many cells and clones; (2) it is same as the serum medium in the growth of the cell and the expression of the product; (3) it support the long heritable culture; (4) it is benefit for the isolation of the product because it contains less protein; (5) it is cheap. We can get the high density of the cells and the product concentration using the medium.
Owner:EAST CHINA UNIV OF SCI & TECH

Microbial Compositions and Methods

The present invention comprises compositions and methods for enhancing biological processes, such as plant growth or bioremediation. For example, the present invention comprises compositions and methods for effectively remediating chemical and organic wastes and reducing the environmental risk from manure, septic, sewage, oil pollution, and other contaminants.
Owner:CONCENTRIC AG CORP

MOSFET device with in-situ doped, raised source and drain structures

InactiveUS6858903B2Lack of selectivityReduces deposition rateSolid-state devicesSemiconductor/solid-state device manufacturingPhysicsMOSFET
A process for manufacturing an FET device. A semiconductor substrate is covered with a gate dielectric layer and with a conductive gate electrode formed over the gate dielectric. Blanket layers of silicon oxide may be added. An optional collar of silicon nitride may be formed over the silicon oxide layer around the gate electrode. Two precleaning steps are performed. Chemical oxide removal gases are then deposited, covering the device with an adsorbed reactant film. The gate dielectric (aside from the gate electrode) is removed, as the adsorbed reactant film reacts with the gate dielectric layer to form a rounded corner of silicon oxide at the base of the gate electrode. One or two in-situ doped silicon layers are deposited over the source / drain regions to form single or laminated epitaxial raised source / drain regions over the substrate protruding beyond the surface of the gate dielectric.
Owner:AURIGA INNOVATIONS INC +1

Non protein culture medium adapted to large-scale culture of NSO cell and production of antibody

The invention relates to a protein-free medium suitable for large-scale cultivation and antibody production of NSO cells, which is a protein-free medium provided with clear chemical compositions. The compositions of the protein-free medium comprises 21 types of amino acid, 13 types of vitamin, 8 types of salt, 4 types of fat, 6 types of microelement, progesterone, sodium bicarbonate, Hepes, glucose, a citric acid, taurine, thymidine, adenine, hypoxanthine, phenol red, mercaptoethanol and Pluronic F-68. The protein-free medium has the advantages that the protein-free medium can support long-term subculture of the NSO cells without the necessity of adaptation, can well support normal growth and antibody expression of the NSO cells, does not contain proteins, various hydrolysate and animal origin compositions, has clear chemical compositions, is favorable for separation and purification of products, improves the quality of medical and biological products, and is convenient to prepare and suitable for mass production and application.
Owner:EAST CHINA UNIV OF SCI & TECH
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