Non-serum culture medium for multiple animal cell large-scale culture

Abstract

The invention opened non-serum mediums which can proper to culture many kinds of the animal cells. The character of it is to use the DMEM / F12 as the base medium, then add the growth factor, hormone, the essential amino acid and the microelement. It has the functions: (1) it can support many cells and clones; (2) it is same as the serum medium in the growth of the cell and the expression of the product; (3) it support the long heritable culture; (4) it is benefit for the isolation of the product because it contains less protein; (5) it is cheap. We can get the high density of the cells and the product concentration using the medium.

Description

technical field [0001] The invention relates to a serum-free medium used for producing biological products such as antibodies, vaccines, and gene recombinant proteins during large-scale high-density culture of animal cells. technical background [0002] Animal cell culture has been widely used in the production of various biologically active substances such as monoclonal antibodies, virus vaccines, virus vectors, immune regulators, growth factors, specific tumor antigens, and various gene recombinant protein drugs. Add a certain amount (5%-10%) of bovine serum, which contains growth factors, hormones, carrier proteins, adhesion factors, trace elements and other nutrients required for cell growth, which can greatly promote cell growth and product expression. [0003] However, the application of serum also brings many disadvantages: [0004] (1) Serum is susceptible to contamination by viruses, mycoplasma or other pathogens; [0005] (2) Differences between different batche...

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Problems solved by technology

[0004] (1) Serum is susceptible to contamination by viruses, mycoplasma or other pathogens;

[0005] (2) Differences between different batches of serum cause differences between product batches;

[0006] (3) The existence of a large amount of serum protein increases the difficulty of downstream separation and purification, which increases the cost and reduces the recovery rate;

[0007] (4) It is difficult to completely remove some serum proteins by means of separation and purification, which seriously affects the final quality of the product

[0012] (1) Due to the high price, it is only suitable for small-scale use in laboratories; it is not suitable for large-scale bioreactors;

[0013] (2) The medium is not optimized for cells, and the supported cell density is low, resulting in low yield in the production process;

[0014] (3) Although some media can support cell growth well, it often leads to the reduction or even loss of the ability of cells to express products;

[0015] (4) The medium is highly specific to cells, and usually a medium is only suitable for one cell line or cell line, but not for other cell lines or cell lines

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