Non protein culture medium adapted to large-scale culture of NSO cell and production of antibody

Abstract

The invention relates to a protein-free medium suitable for large-scale cultivation and antibody production of NSO cells, which is a protein-free medium provided with clear chemical compositions. The compositions of the protein-free medium comprises 21 types of amino acid, 13 types of vitamin, 8 types of salt, 4 types of fat, 6 types of microelement, progesterone, sodium bicarbonate, Hepes, glucose, a citric acid, taurine, thymidine, adenine, hypoxanthine, phenol red, mercaptoethanol and Pluronic F-68. The protein-free medium has the advantages that the protein-free medium can support long-term subculture of the NSO cells without the necessity of adaptation, can well support normal growth and antibody expression of the NSO cells, does not contain proteins, various hydrolysate and animal origin compositions, has clear chemical compositions, is favorable for separation and purification of products, improves the quality of medical and biological products, and is convenient to prepare and suitable for mass production and application.

Description

【Technical field】 [0001] The invention relates to the technical field of medium research and development of modern biotechnology, specifically, a protein-free medium suitable for large-scale cultivation of NSO cells and antibody production. 【technical background】 [0002] Modern biotechnology is one of the most important cutting-edge technologies in the 21st century. Among them, the use of animal cell culture technology to produce growth factors, vaccines and monoclonal antibodies with important medical value has become an important part of the high-tech pharmaceutical and biotechnology industry. In order to perfect the animal cell culture technology, people pay great attention to the research on the culture medium of animal cells, because the culture medium is one of the foundation and key elements of the animal cell culture technology. In the past few decades, with the development of science and technology, animal cell culture has gone through several stages of adopting s...

AI Technical Summary

Problems solved by technology

In another report, Jinyou Zhang et al. successfully developed a protein-free medium suitable for the growth of NSO cells, but due to its complex composition and the inclusion of an expensive commercial mixed lipid with undefined chemical composition, therefore, Not suitable for large-scale culture of NSO cells and antibody production

[0010] At present, in the field of protein-free medium technology, commercial serum-free and protein-free medium represented by Gibco, Hyclone, JRH, etc. have come out one after another. They have good effects on some cell lines; however, they also have some obvious disadvantages, and there will be many difficulties in using them in some researches and large-scale cell culture and antibody production processes. The main problems are: the first First, the price is expensive, and it is only suitable for small-scale research in some laboratories, not suitable for large-scale cell culture and antibody production; second, most of the components are complex, and uncertain chemical components are added, which brings difficulties to the scientific research and production process; Third, although some media can support cell growth well, they reduce or even lose the ability of cells to express products.

Images