Method for extracting isoliquirtigenin from licorice

A technology of isoliquiritigenin and licorice, applied in the separation/purification of carbonyl compounds, preparation of carbonyl compounds by hydrolysis, organic chemistry, etc., can solve problems such as not considering liquiritigenin

Inactive Publication Date: 2008-12-24
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Flavonoid glycosides were hydrolyzed by acid to obtain corresponding aglycones. Fu Yujie reported in Chinese Journal of Pharmaceutical Sciences, 2007, 42 (11): 818-821 that under acidic conditions, isoliquiritin was hydrolyzed into isoliquiritigenin, thereby improving the quality of isoliquiritigenin. However, the report did not consider the hydrolysis of glycyrrhizinic acid to generate liquiritigenin, and the factor that liquiritigenin can be converted into isoliquiritigenin under alkaline conditions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1: Glycyrrhiza was crushed, passed through a 30-60 mesh sieve, 1 kg of licorice powder was taken, 3000 mL of 1.5 mol / L hydrochloric acid was added, nitrogen was filled in the reaction kettle, and the acid hydrolyzate was heated to 100° C. for 60 minutes for hydrolysis. After the completion of hydrolysis, slowly add sodium hydroxide particles to adjust the pH value to 14, alkalinize the solution at 100°C for 10 minutes, adjust the pH value of the solution to 10, dry, and extract twice with ethanol. The ethanol extract was concentrated, and the polyamide column chromatography was eluted with a gradient of 60%-90% ethanol to obtain bright yellow crystals of isoliquiritigenin.

Embodiment 2

[0013] Example 2: Glycyrrhizae was crushed, passed through a 60-80 mesh sieve, 1 kg of licorice powder was taken, 20,000 mL of 1 mol / L hydrochloric acid was added, the reaction kettle was filled with argon, and the acid hydrolyzate was heated to 75° C. for 120 minutes for hydrolysis. After the hydrolysis is completed, slowly add potassium hydroxide particles to adjust the pH value to 13, alkalinize the solution at 30°C for 60 minutes, adjust the pH value of the solution to 7, dry it, and extract with ethanol for 3 times. The ethanol extract was concentrated, and bright yellow crystals of isoliquiritigenin were obtained by silica gel column chromatography.

Embodiment 3

[0014] Example 3: Glycyrrhizae is crushed, passed through a 60-80 mesh sieve, 1kg of licorice powder is taken, 5000mL of 2mol / L hydrochloric acid is added, a mixed gas of argon and nitrogen is filled in the reaction kettle, and the acid hydrolyzate is heated to 60°C Hydrolyzed for 180 minutes. After the hydrolysis is completed, slowly add potassium hydroxide particles to adjust the pH value to 12, alkalinize the solution at 50°C for 100 minutes, adjust the pH value of the solution to 9, dry it, and extract with ethanol for 3 times. The ethanol extract was concentrated, and bright yellow crystals of isoliquiritigenin were obtained by silica gel column chromatography.

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PUM

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Abstract

The invention relates to a method for extracting isoliquiritigenin from iquorice. The method uses a property of flavonone which can be transformed into chalcone in the presence of alkali, an acid hydrolysis product of liquidritin is transformed into the isoliquiritigenin, thereby obviously improving the yield of extracting the isoliquiritigenin. With the method, the yield of extracting the isoliquiritigenin is 32 times of that of ethanol ultrasonic extraction, and 1.7 times of that of acid hydrolysis extraction.

Description

Technical field: [0001] The invention relates to a method for extracting isoliquiritigenin from licorice. Background technique: [0002] Isoliquiritigenin is one of the most expensive active ingredients in licorice, which has anti-oxidation, anti-ulcer and anti-HIV effects. It has outstanding activities of inhibiting monoamine oxidase, anti-cancer, anti-platelet aggregation and relaxing tracheal smooth muscle. Some countries have already traded isoliquiritigenin as a raw material drug. According to the author's literature research and market research, the demand for isoliquiritigenin in the international market is large, coupled with the high price, it is not difficult to speculate that a reasonable extraction process of isoliquiritigenin will bring huge economic benefits. [0003] Liquiritin and isoliquiritin are flavonoid glycosides, liquiritigenin is the aglycone of liquiritin, and isoliquiritigenin is the aglycone of isoliquiritin. Flavonoid glycosides were hydrolyzed ...

Claims

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Application Information

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IPC IPC(8): C07C49/835C07C45/78C07C45/42
Inventor 韩博陈文王新春严欢赵洁李乐
Owner SHIHEZI UNIVERSITY
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