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II-type collagen joint cartilage fluid and preparation method thereof

A technology of articular cartilage and repair fluid, which is applied to bone diseases, medical formulas, medical preparations with non-active ingredients, etc., and can solve the problems of non-toxic, sterile, non-pyrogenic Type II collagen joint repair fluid research reports, etc. problem, achieve the effect of improving joint motion function, preventing aggravation of the disease, and relieving pain

Active Publication Date: 2010-08-25
GUANGZHOU TRAUER BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of type II collagen for intra-articular injection for the treatment of cartilage defects and arthritis has been reported in domestic literature, but there is no non-toxic, sterile, non-pyrogenic high-quality drug prepared by the ratio of type II collagen and other polysaccharide auxiliary materials. Research Report on Concentrated Type II Collagen Joint Repair Liquid

Method used

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  • II-type collagen joint cartilage fluid and preparation method thereof
  • II-type collagen joint cartilage fluid and preparation method thereof
  • II-type collagen joint cartilage fluid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A preferred embodiment of the non-toxic, non-pyrogenic and highly active type II collagen preparation method of the present invention is:

[0033] (1) Raw material handling

[0034] The hyaline cartilage of pig joints is removed through fascial fat removal→cleaning and soaking→toxic substance treatment→disinfection to remove all bacteria, viruses and other microorganisms.

[0035] The specific method process is as follows:

[0036] The fascial adipose tissue in the porcine articular hyaline cartilage was completely removed with a blade, and sliced ​​into thin slices. Degrease and serum components with 20% NaCl+0.05M Tris (pH7.5), wash thoroughly until no lipid floats, and the liquid has no foam. After being treated with 0.5% chlorhexidine by volume at room temperature for 30 minutes, it was fully washed. Treat with 0.1% peracetic acid by volume at room temperature for 15 minutes, wash and pack into sealed bags, then send to Co 60 Sterilize by irradiation with a dose...

Embodiment 2

[0065] A preferred embodiment of the non-toxic, non-pyrogenic and highly active type II collagen preparation method of the present invention is:

[0066] (1) Raw material handling

[0067] The hyaline cartilage of pig joints is removed through fascial fat removal→cleaning and soaking→toxic substance treatment→disinfection to remove all bacteria, viruses and other microorganisms.

[0068] The specific method process is as follows:

[0069] The fascial adipose tissue in the porcine articular hyaline cartilage was completely removed with a blade, and sliced ​​into thin slices. Degrease and serum components with 20% NaCl+0.05M Tris (pH7.5), wash thoroughly until no lipid floats, and the liquid has no foam. After treating with 0.1% chlorhexidine at room temperature for 60 minutes by volume percentage, fully wash. Treat with 1% peracetic acid by volume at room temperature for 20 minutes, wash and pack into sealed bags, then send to Co 60 Sterilize by irradiation with a dose of 3...

Embodiment 3

[0098] A preferred embodiment of the non-toxic, non-pyrogenic and highly active type II collagen preparation method of the present invention is:

[0099] (1) Raw material handling

[0100] The hyaline cartilage of pig joints is removed through fascial fat removal→cleaning and soaking→toxic substance treatment→disinfection to remove all bacteria, viruses and other microorganisms.

[0101] The specific method process is as follows:

[0102] The fascial adipose tissue in the porcine articular hyaline cartilage was completely removed with a blade, and sliced ​​into thin slices. Degrease and serum components with 20% NaCl+0.05M Tris (pH7.5), wash thoroughly until no lipid floats, and the liquid has no foam. After being treated with 5% chlorhexidine at room temperature for 10 minutes by volume, it was fully washed. Treat with 3% peracetic acid by volume at room temperature for 5 minutes, wash and pack into sealed bags, then send to Co 60 Sterilize by irradiation with a dose of 1...

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Abstract

The invention provides II-type collagen joint cartilage fluid and a preparation method thereof. Bacteria, viruses and other hazardous substances of the joint cartilage are removed through degreasing-> serum removing-> surfactant treatment-> oxidant treatment-> disinfection and other links. Hybrid protein and other denatured protein of the cartilage are removed through acid hydrolysis, salt precipitation, dialysis and other processes, and high-purity and high-activity II-type collagen solution is prepared. Non-toxin and non-heat raw materials and auxiliary materials and isotonic solution are mixed in an appropriate ratio, and filled in a sterile non-heat disposable injector, to prepare II-type collagen joint cartilage fluid for disposable injection. Other components of the II-type collagen joint cartilage fluid comprise the auxiliary materials and the isotonic solution. The II-type collagen joint cartilage fluid can play the roles of lubrication, pain relieving and joint activity degree improving to the injured joints, fundamentally realizes the internal repair to the cartilage tissue, and is applicable to a patient with various joint cartilage injuries and all types of arthritis.

Description

technical field [0001] The invention relates to the field of joint orthopedic medical supplies, in particular to a type II collagen articular cartilage repair liquid and a preparation method thereof. Background technique [0002] Articular cartilage damage refers to cartilage wear and degeneration caused by the aging population and cartilage damage caused by disease, trauma, and sports injuries. The self-repair function of articular cartilage is very limited, and osteoarthritis often occurs in damaged joints, and the disease is easy to gradually aggravate, leading to the loss of joint function. It is mainly related to the following factors: 1) There is no blood vessel in the cartilage; 2) The chondrocytes are fixed in a dense solid matrix composed of collagen and proteoglycan; 3) The mitotic ability of mature chondrocytes is extremely low, and they cannot produce the cells required for repair. Chondrocytes and cartilage matrix, namely collagen and proteoglycan; 4) Participa...

Claims

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Application Information

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IPC IPC(8): A61K38/39A61K47/36A61K47/26A61P19/04
Inventor 佟刚雷静黄冬晶陈淡嫦
Owner GUANGZHOU TRAUER BIOTECH
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