The invention relates to a method for purifying collagen derived from
pelodiscus sinensis. The method is characterized by comprising the steps of soaking,
fat removal and enzymolysis; in an enzymolysis process, carinas of the
pelodiscus sinensis, of which impurities are removed, are put into
acetic acid with the concentration of 0.5mol / L, wherein the ratio of the carinas to the
acetic acid is 1:25, pepsase enzymatic
hydrolysate is added for enzymolysis treatment, and extraction is carried out at 4-10 DEG C for 24-48 h, so that a
pelodiscus sinensis carina collagen coarse extracting solution is obtained; in a
salting-out purification process, the coarse extracting solution is dissolved into a
Tris-HCL
buffer solution containing NaCl, the
mixed solution is centrifuged to obtain supernatant, NaCl is added into the supernatant,
salting-out is carried out,
centrifugation is carried out again, and precipitates and the supernatant are respectively collected; and precipitates are dissolved into
acetic acid with the concentration of 0.5 mol / L, and freeze-
drying is carried out after
dialysis, so that the pelodiscus sinensis collagen freeze-dried product is obtained. Compared with the prior art, the method provided by the invention has the advantages that collagen extracted through enzymolysis is purified through a
salting-out purification method, so that no environment
pollution is realized, the extraction efficiency is high and the obtained product has good biological characteristics.