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Spleen polypeptide extract, its preparing process and use

A technology of extracts and small molecule peptides, applied in the direction of peptide source, drug combination, drug delivery, etc., to achieve the effect of exact curative effect, improvement of physical weakness and low cost

Active Publication Date: 2005-07-06
融致丰生制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] As we all know, there are many nutrients and active ingredients needed by the human body in mammals, and there are many reports on the extraction of active peptides from the heart, liver and muscle tissue of mammals

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] step 1):

[0045] A) Take 10kg of fresh healthy calf spleen, remove impurities such as fascia and adipose tissue, wash with water for injection, add 10L water for injection, homogenate, adjust the pH value to 3.8, freeze at -20°C, take out after freezing and place in a water bath at 15°C Thaw in medium, repeat freeze-thaw 2 times.

[0046] B) Centrifuge the homogenate after the last melting, the centrifugation condition is 4000 rpm, the centrifugation time is 15 minutes, the supernatant is filtered through the pulp, and the filtrate is adjusted to pH 6.5, heated at 80°C for 15 minutes, cooled After reaching 35°C, centrifuge at 4000 r / min for 20 minutes, filter the pulp to obtain the supernatant;

[0047] C) Freeze at -20°C, take out after freezing and melt in a 12°C water bath, after the pulp is filtered, the supernatant is ultrafiltered once with an ultrafiltration membrane (U.S. millipore company, standard ultrafiltration system) with a molecular weight cut-off o...

Embodiment 2

[0051] step 1):

[0052] A) Take 10kg of fresh healthy pig spleen, remove impurities such as fascia and adipose tissue, wash with water for injection, add 20L water for injection, homogenate, adjust the pH value to 4.2, freeze at -18°C, take out in a water bath at 10°C after freezing Thaw, repeat freeze-thaw once.

[0053] B) Centrifuge the homogenate after the last melting, the centrifugation condition is 3000 rpm, the centrifugation time is 30 minutes, the supernatant is filtered through the pulp, and the filtrate is adjusted to pH 7.0, heated at 70°C for 20 minutes, cooled After reaching 20°C, centrifuge at 4,000 rpm for 30 minutes, filter the pulp to obtain a clear liquid, and freeze at -18°C;

[0054] C) after freezing, take out and melt in a water bath at 25°C. After the pulp is filtered, the supernatant is first ultrafiltered once with an ultrafiltration membrane (U.S. millipore company, standard ultrafiltration system) with a molecular weight cut-off of 10,000, and th...

Embodiment 3

[0058] step 1):

[0059] A) Take 10kg of fresh healthy sheep spleen, remove impurities such as fascia and adipose tissue, wash it with water for injection, add 15L water for injection, homogenate, adjust the pH value to 3.0, freeze at -15°C, take it out in a water bath at 28°C after freezing Thaw, repeat freeze-thaw 4 times.

[0060] B) Centrifuge the homogenate after the last melting, the centrifugation condition is 3500 rpm, the centrifugation time is 25 minutes, the supernatant is filtered through the pulp, and the filtrate is adjusted to pH 6.0, heated at 75°C for 15 minutes, cooled After reaching 25°C, centrifuge at 3500 rpm for 30 minutes, filter the pulp to obtain a supernatant, and freeze at -15°C.

[0061] C) after freezing, take out and melt in a water bath at 15°C. After the pulp is filtered, the supernatant is first ultrafiltered once with an ultrafiltration membrane (U.S. millipore company, standard ultrafiltration system) with a molecular weight cut-off of 8000,...

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Abstract

The invention discloses a spleen polypeptide extract extracted from the spleen tissue of mammals other than humans. The mammalian tissue is subjected to fat removal, homogenization, acid adjustment, freezing and thawing, pH adjustment, precipitation, heating, and centrifugation. Filtration, ultrafiltration and other steps to obtain the spleen polypeptide extract; the drug is an immunomodulatory drug that can improve and improve the immune function of the body, and can be used for primary and secondary cellular immune deficiency diseases (such as eczema, thrombocytopenia, multiple Infection syndrome, etc.), respiratory tract and lung infection, habitual cold, chronic hepatitis B, mumps, recurrent aphtha and other diseases, can be used in the treatment of leukopenia, leukemia, aplastic anemia caused by radiotherapy and chemotherapy , Lymphoma and other malignant tumors, to improve the malignant transformation of tumor patients, to improve postoperative or critically ill patients when they are weak.

Description

technical field [0001] The present invention relates to a spleen polypeptide extract, its preparation method and its use, in particular, to a spleen polypeptide extract prepared by extracting and separating from the spleen tissue of mammals other than humans, its preparation method and use . Background technique [0002] As we all know, there are many various nutrients and active ingredients needed by the human body in mammals, and there are many reports on the extraction of active polypeptides from the heart, liver and muscle tissue of mammals. [0003] The spleen is the peripheral immune organ of mammals, and it is also one of the organs with the most vigorous metabolism in animals. Because it is rich in basic cell nutrients and cytokines, especially active ingredients that promote cell metabolism such as polypeptides and nucleic acids and immune Regulatory factors - oligonucleotide peptides, etc., have thus received extensive attention from researchers. The research wor...

Claims

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Application Information

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IPC IPC(8): A61K9/08A61K9/19A61K9/20A61K9/48A61K38/17A61P1/02A61P11/00A61P17/00A61P31/04A61P31/12A61P35/00A61P37/02C07K14/435C07K14/47
Inventor 王建辉
Owner 融致丰生制药有限公司
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