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Spleen extracts, its preparation method and use

A technology of extract and spleen, which is applied in the field of pharmaceutical preparations and achieves the effects of exact curative effect, low cost and high yield

Active Publication Date: 2006-05-03
融致丰生制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] As we all know, there are many nutrients and active ingredients needed by the human body in mammals, and there are many reports on the extraction of active peptides from the heart, liver and muscle tissue of mammals

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] This example provides a spleen extract containing 8.0 mg of polypeptide, 10.2 mg of free amino acid, 2.2 mg of nucleic acid, 260 μg of total sugar and molecular weight less than 6000 Dalton per ml. Its preparation steps are as follows:

[0052] A) Take 10kg of fresh healthy calf spleen, remove impurities such as fascia and adipose tissue, wash with water for injection, add 10L water for injection, homogenate, adjust the pH value to 3.8, freeze at -20°C, take out after freezing and place in a water bath at 15°C The homogenate after the last thaw was centrifuged at 4000 rpm for 15 minutes, and the supernatant was filtered to obtain supernatant I.

[0053] B) Adjust the pH value of the supernatant I to 6.5, heat at 80°C for 15 minutes, cool to 35°C and centrifuge at 4000 rpm for 20 minutes, take the supernatant and filter to obtain Serum II;

[0054] C) Freeze the supernatant II at -20°C, take it out after freezing, and melt it in a water bath at 12°C, and ultrafilter th...

Embodiment 2

[0058] This example provides a spleen extract containing 5.0 mg of polypeptide, 7.0 mg of free amino acid, 1.6 mg of nucleic acid, 180 μg of total sugar and molecular weight less than 8000 Dalton per ml. Its preparation steps are as follows:

[0059] A) Take 10kg of fresh healthy pig spleen, remove impurities such as fascia and adipose tissue, wash with water for injection, add 20L water for injection, homogenate, adjust the pH value to 4.2, freeze at -18°C, take out in a water bath at 10°C after freezing Thaw, repeat freezing and thawing once, centrifuge the homogenate after the last thawing, the centrifugation condition is 3000 rpm, and the centrifugation time is 30 minutes, take the supernatant and filter to obtain supernatant I;

[0060] B) After adjusting the pH value of the supernatant I filtrate to 7.0, centrifuge, the centrifugation condition is 4000 rpm, and the centrifugation time is 30 minutes, take the supernatant and filter to obtain the supernatant II;

[0061] ...

Embodiment 3

[0065] This example provides a spleen extract containing 7.0 mg of polypeptide, 8.0 mg of free amino acid, 3.0 mg of nucleic acid, 360 μg of total sugar and molecular weight less than 5000 Dalton per ml. Its preparation steps are as follows:

[0066] A) Take 10kg of fresh healthy sheep spleen, remove impurities such as fascia and adipose tissue, wash it with water for injection, add 15L water for injection, homogenate, adjust the pH value to 3.0, freeze at -15°C, take it out in a water bath at 28°C after freezing Thaw, repeat freezing and thawing 4 times, centrifuge the homogenate after the last thawing, the centrifugation condition is 3500 rpm, the centrifugation time is 25 minutes, take the supernatant and filter to obtain supernatant I;

[0067] B) Adjust the pH value of the supernatant I filtrate to 6.0, heat at 75°C for 15 minutes, cool to 25°C and centrifuge at 3500 rpm for 30 minutes, then filter the supernatant, Clear solution II;

[0068] C) Supernatant II was froze...

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Abstract

The invention discloses a spleen extract prepared from mammalsí» spleens through fat-removing, homogenating, comminuting, settling and hyperfiltrating, and comprises the following constituents: polypeptides, free amino acid, nucleic acid and total sugar. The spleen extract can be used for preparing medicament for the treatment of cell immunodeficiency diseases, respiratory tract and lung infection, chronic hepatitis B, mumps, aphtae, leucopenia, leukemia, regenerative anemia, and malignant tumour.

Description

【Technical field】 [0001] The present invention relates to a spleen extract, its preparation method and its use, in particular to a spleen extract prepared by extracting and separating from the spleen tissue of mammals other than humans, its preparation method and its use, belonging to field of pharmaceutical preparations. 【Background technique】 [0002] As we all know, there are many various nutrients and active ingredients needed by the human body in mammals, and there are many reports on the extraction of active polypeptides from the heart, liver and muscle tissue of mammals. [0003] The spleen is the peripheral immune organ of mammals, and it is also one of the organs with the most vigorous metabolism in animals. Because it is rich in basic cell nutrients and cytokines, especially active ingredients that promote cell metabolism such as polypeptides, free amino acids, Nucleic acid and various sugars have received extensive attention from researchers. The research work o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/08A61K9/19A61K9/20A61K9/48A61K35/55A61K38/17A61P11/00A61P17/00A61P37/02
Inventor 郭智华
Owner 融致丰生制药有限公司
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