The invention relates to the field of health-care, and in particular relates to a culture method of hair follicle stem cell for treating baldness, as well as a syringe. The culture method comprises the following steps: A, digesting excised hair follicles by utilizing separase; B, cutting bugle of follicles; C, digesting the follicle bugle by utilizing pancreatin; D, centrifugally collecting the follicle bulge obtained in the step C to obtain cells; E, culturing the cells in DEME (Dulbecco's Modified Eagle's Medium) culture solution, and primarily separating; F, culturing the product which is obtained in primary separation of the step E in a culture dish; and G, centrifugally collecting upper unattached keratinocyte and follicle fibroblast which are cultured in the step F, and culturing to obtain hair follicle stem cells. The syringe comprises a hair follicle stem cell extraction component and a syringe needle, wherein the hair follicle stem cell extraction component is used for quantitatively extracting hair follicle stem cells, and connected with the syringe needle; and the syringe needle is bent and blunt. According to the method and the syringe, hair growth under baldness situation can be improved.