51 results about "Acetyl phosphate" patented technology

The invention relates to a recombinant yeast, a construction method thereof and application thereof for producing tyrosol and a derivative. The recombinant yeast is acquired by introducing an exogenous fructose-6-phosphate phosphoketolase into an engineered yeast cell, and the engineered yeast cell is a yeast cell having a metabolic pathway for the synthesis of tyrosol via erythrose-4-phosphate and phosphoenolpyruvate. It is disclosed for the first time that in the process of expressing the fructose-6-phosphate phosphoketolase in the yeast, fructose-6-phosphate is catalyzed into erythrose-4- phosphate and acetyl phosphate while synthesizing 1,6-fructose diphosphate, xylulose-5-phosphate is catalyzed into glyceraldehydes-3-phosphate and acetyl phosphate, which alters the distribution of carbon metabolism in yeast, and enhances the synthesis of erythrose-4-phosphate, an important intermediate of tyrosol biosynthesis. The metabolic pathway of synthetic tyrosol is optimized, and the yieldof derivatives such as tyrosol and hydroxytyrosol is improved.

The invention relates to a preparation method of glutamine dipeptide. Escherichia coli is used for expressing amino acid ligase from bacillus subtilis and acetokinase from clostridium acetobutylicum respectively, and a double-enzyme coupling system is formed through mixing after purification. The amino acid ligase is used for catalyzing L-alanine and L-glutamine to generate the glutamine dipeptide, meanwhile ADP is formed along with ATP dephosphorylation, and the acetokinase is used for catalyzing acetyl phosphate and the ADP to form ATP, so that cyclic regeneration of the ATP is realized. By using the double-enzyme coupling system, 32.5 mM of glutamine dipeptide can be obtained through reaction for 8 h under appropriate reaction conditions, and the molar conversion rate is 64.5%. The production method of the glutamine dipeptide provided by the invention has the advantages of low raw material cost, short enzymatic conversion time, simple and convenient operation, low production cost and the like, and has relatively high industrial application value.

Microorganisms comprising modifications for producing pyruvate, ethanol, and other compounds. The microorganisms comprise modifications that reduce or ablate activity of one or more of pyruvate dehydrogenase, 2-oxoglutarate dehydrogenase, phosphate acetyltransferase, acetate kinase, pyruvate oxidase, lactate dehydrogenase, cytochrome terminal oxidase, succinate dehydrogenase, 6-phosphogluconate dehydrogenase, glutamate dehydrogenase, pyruvate formate lyase, pyruvate formate lyase activating enzyme, and isocitrate lyase. The microorganisms optionally comprise modifications that enhance expression or activity of pyruvate decarboxylase and alcohol dehydrogenase. The microorganisms are optionally evolved in defined media to enhance specific production of one or more compounds. Methods of producing compounds with the microorganisms are provided.