Ammonia ion diagnosis/measuring reagent kit and ammonia ion concentration determination method
A kit and ion technology, applied in the field of measuring ammonia concentration, ammonia diagnosis/determination kit, can solve the problems such as undiscovered blood ammonia measurement method
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Embodiment 1
[0042] The ammonia (ion) diagnosis / measurement reagent of the present embodiment is a single reagent, comprising:
[0043] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0044] Stabilizer 500mmol / L
[0045] Reduced coenzyme 0.25mmol / L
[0046] Glutamine Synthetase 6000U / L
[0047] Acetate kinase 8000U / L
[0048] Aldehyde dehydrogenase 10000U / L
[0049] Glutamic acid 10mmol / L
[0050] Adenosine triphosphate 3mmol / L
[0051] Acetyl phosphate 5mmol / L
[0052] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.
[0053] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the ammonia (ion) sample to the reagent is 1 / 25, the reaction direction is negative reaction (downward reactio...
Embodiment 2
[0056] The ammonia (ion) diagnosis / measurement reagent of the present embodiment is a double reagent, comprising:
[0057] Reagent 1
[0058] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0059] Stabilizer 50mmol / L
[0060] Reduced coenzyme 0.25mmol / L
[0061]Glutamic acid 10mmol / L
[0062] Adenosine triphosphate 3mmol / L
[0063] Acetyl phosphate 5mmol / L
[0064] Reagent 2
[0065] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0066] Stabilizer 500mmol / L
[0067] Glutamine Synthetase 6000U / L
[0068] Acetate kinase 8000U / L
[0069] Aldehyde dehydrogenase 10000U / L
[0070] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.
[0071] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test subwavelength 405nm, tested ammonia (ion) sample and reag...
Embodiment 3
[0074] The ammonia (ion) diagnosis / measurement reagent of the present embodiment is three reagents, comprising:
[0075] Reagent 1
[0076] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0077] Stabilizer 50mmol / L
[0078] Reduced coenzyme 0.25mmol / L
[0079] Glutamic acid 10mmol / L
[0080] Adenosine triphosphate 3mmol / L
[0081] Acetyl phosphate 5mmol / L
[0082] Reagent 2
[0083] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0084] Stabilizer 500mmol / L
[0085] Acetate kinase 8000U / L
[0086] Aldehyde dehydrogenase 10000U / L
[0087] Reagent 3
[0088] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0089] Stabilizer 500mmol / L
[0090] Glutamine Synthetase 6000U / L
[0091] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.
[0092] When measuring the ammonia (ion) concentration, set it on the automatic biochem...
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