32 results about "Erythrose" patented technology

The invention relates to a recombinant yeast, a construction method thereof and application thereof for producing tyrosol and a derivative. The recombinant yeast is acquired by introducing an exogenous fructose-6-phosphate phosphoketolase into an engineered yeast cell, and the engineered yeast cell is a yeast cell having a metabolic pathway for the synthesis of tyrosol via erythrose-4-phosphate and phosphoenolpyruvate. It is disclosed for the first time that in the process of expressing the fructose-6-phosphate phosphoketolase in the yeast, fructose-6-phosphate is catalyzed into erythrose-4- phosphate and acetyl phosphate while synthesizing 1,6-fructose diphosphate, xylulose-5-phosphate is catalyzed into glyceraldehydes-3-phosphate and acetyl phosphate, which alters the distribution of carbon metabolism in yeast, and enhances the synthesis of erythrose-4-phosphate, an important intermediate of tyrosol biosynthesis. The metabolic pathway of synthetic tyrosol is optimized, and the yieldof derivatives such as tyrosol and hydroxytyrosol is improved.

There is provided the use as reducing agents of alpha-hydroxycarbonyl compounds capable of forming cyclic dimers. There is also provided corresponding methods of reducing reducible compounds, particularly reduction-activated prodrugs . Examples of the alpha-hydroxycarbonyl compounds used are dihydroxyacetone, glycolaldehyde , glyceraldehyde, erythrose, xylulose, erythrulose or 3-hydroxy-2-butanone.

The invention discloses a corynebacterium glutamicum engineering strain for biosynthesis of rare sugar, and a building method and application thereof, and discloses a corynebacterium glutamicum recombination strain SY10. An experiment proves that rare ketose and deoxidized ketose can be synthesized by adopting a plurality of hydroxyaldehydes and glucoses as substrates by the strain in a fermentation manner, for example, D-erythrulose can be synthesized by adopting formaldehyde and glucose as substrates, L-xylulose can be synthesized by adopting glycolaldehyde and glucose as substrates, D-sorbose and D-psicose can be synthesized by adopting D-glyceraldehyde and glucose as substrates, L-fructose can be synthesized by adopting L-glyceraldehyde and glucose as substrates, and 3R, 4S, 5R, 6R-heptose and 3R, 4R, 5R, 6R-heptose can be synthesized by adopting D-erythrose and glucose as substrates. Therefore, the corynebacterium glutamicum recombination strain SY10 disclosed by the invention can be applied to the field of production of the rare ketose and the deoxidized ketose by whole-cell fermentation; the produced rare ketose and deoxidized ketose have broad application prospects in the industries such as a food, a medicine and the like.

The invention provides methods for the production of erythrose and / or erythritol. Preferably, the methods include the step of electrolytic decarboxylation of a ribonic acid or arabinonic acid reactant to produce erythrose. Optionally, the reactant can be obtained from a suitable hexose sugar, such as allose, altrose, glucose, fructose or mannose. The erythrose product can be hydrogenated to produce erythritol.

The invention provides a new method for the preparation of high value-added chemicals by selective photocatalytic oxidation of glucose, that is, the rutile phase TiO2 loaded with metal promoters 2 As a photocatalyst, glucose can be directly converted into arabinose and erythrose through photocatalytic dehydrogenation and oxidation at room temperature. The yields of arabinose and erythrose prepared by the method are above 45mol% and 26mol% respectively. The reaction conditions are mild, no need to add acid, alkali and organic solvent, and no high temperature and high pressure conditions.

The invention relates to a preparation method of 4-phosphoric acid erythrose, comprising the following steps: immobilizing heat-resistant transketolase at the temperature of 55-65 DEG C; and then taking 6-fructose phosphate and 3-glyceraldehyde phosphate as raw materials to prepare the 4-phosphoric acid erythrose by enzymatic reaction. In the preparation method, the heat-resistant and specifically transformed transketolase of the 4-phosphoric acid erythrose is prepared by adopting a molecular biology technology, and the 6-fructose phosphate and the 3-glyceraldehyde phosphate are taken as the raw materials to efficiently prepare the 4-phosphoric acid erythrose by adopting an immobilized enzyme technology. The preparation method is simple and practical.

The invention provides a mud snail health beverage and a preparation method thereof. The raw material formula of the beverage is calculated by weight percentage: 5-15% of mud snail enzymatic hydrolysis solution, 3-10% of red nan fruit juice, and 0.2% of erythritol ~1.8%, 3~10% of Artemisia annua buds, 2~8% of Tudinggui, and the balance is drinking water; thereby, the present invention not only retains the original flavor of mud snails at full price and does not lose biologically active components, but also solves the problem of The problem of fishy smell of mud snails and restrictions on eating environment and eating methods, supplemented by red nan fruit juice, erythrose, artemisia buds, soil dinggui, etc. The physiologically active substances and nutritional and health ingredients are fully introduced into beverage products , produce synergistic effect and complement each other. Its color is light reddish, fragrant and mellow, delicious, and unique in flavor. It has better nutritional and health value and is easier for the human body to fully digest and absorb. Regular consumption can improve the body's immunity, prevent diseases, and strengthen the body. Effect.

The present invention relates to the preparation of small molecule metabolites glucose-6-phosphate, erythrose-4-phosphate, xanthine and phosphatidylcholine PC (33:1) in tissue samples as combined metabolic markers for diagnosing subjects A new application of the kit in patients with lung cancer and benign pulmonary nodules. The present invention also relates to a kit for detecting patients with lung cancer and benign pulmonary nodules in a subject, by detecting the relative concentrations of the above-mentioned combined markers in tissue samples from the subject, and calculating the combined markers based on a binary logistic regression equation The P value of the marker variable is then based on the determined cutoff value (cutoff) to diagnose patients with lung cancer and benign pulmonary nodules. In addition, the combined marker also has good diagnostic ability for patients with early lung cancer and benign pulmonary nodules. The kit can realize highly sensitive and efficient detection of several small molecule metabolites involved in the present invention, and has the characteristics of low detection cost and good repeatability.