106 results about "Xylulose" patented technology

The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolised by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi.

The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolised by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi.

The invention relates generally to the field of industrial microbiology and butanol production from sources of 5-carbon sugars such as lignocellulosic hydrolysates. More specifically, the invention relates to the use of an xylulose or xylulose-5-phosphate-producing enzyme and micro-aerobic or anaerobic conditions to increase butanol production from such sugars and recovery of said butanol through ins situ product recovery methods.

The present invention relates to a method for obtaining a recombinant yeast of Saccharomyces cerevisiae, which ferments lignocellulose raw materials to ethanol, including introducing DNA into a yeast so as to cause the yeast to have introduced genes encoding xylose reductase, xylitol dehydrogenase and xylulokinase.

The invention discloses recombinant saccharomyces cerevisiae for producing dammarenediol and protopanoxadiol using xylose and a construction method. The construction method comprises the steps of replacing a promoter of a saccharomyces cerevisiae xylulokinase gene XKS1 with a promoter PFBA1 by virtue of a homologous recombination method, introducing xylose reductase XYL1 and a xylitol dehydrogenase XYL2 expression cassette, increasing the activities of transketolase TKL1 and transaldolase TAL1 so as to obtain recombinant bacteria 1, introducing a farnesyl-diphosphate farnesyltransferase gene ERG9, a squalene monooxygenase gene ERG1 and a dammarenediol synthase gene DS into the recombinant bacteria 1 so as to obtain recombinant bacteria 2, and introducing a nicotinamide adenine dinucleotide-hydroxymethylglutaryl coenzyme A reductase gene NADH-HMGr, farnesyl diphosphatesynthase ERG20 and a protopanoxadiol synthase-cytochrome P450 reductase fusion protein gene PPDS-ATR1 into the recombinant bacteria 2, so as to obtain recombinant bacteria 3. According to the recombinant saccharomyces cerevisiae, dammarenediol and protopanoxadiol can be artificially synthesized by virtue of xylose.

The invention discloses a method for improving the sugar utilization rate of clostridium acetobutylicum in fermentation of mixed sugar. The method comprises the following steps of: performing gene engineering modification on clostridium acetobutylicum, so that compared with wild type clostridium acetobutylicum, the clostridium acetobutylicum has the advantages that expression of g1cG gene can be inhibited, and the expression and activity of xylose transportprotein, xylose isomerase, and / or xylulokinase can be improved; and applying the obtained clostridium acetobutylicum which is subjected to gene engineering to fermentation of sugar. By the method, more xylose and arabinose can be used by clostridium acetobutylicum in the fermentation of the mixed sugar, a solvent product with high concentration can be produced, and the product yield can be improved; and the method has excellent industrial application prospect.

The invention discloses a corynebacterium glutamicum engineering strain for biosynthesis of rare sugar, and a building method and application thereof, and discloses a corynebacterium glutamicum recombination strain SY10. An experiment proves that rare ketose and deoxidized ketose can be synthesized by adopting a plurality of hydroxyaldehydes and glucoses as substrates by the strain in a fermentation manner, for example, D-erythrulose can be synthesized by adopting formaldehyde and glucose as substrates, L-xylulose can be synthesized by adopting glycolaldehyde and glucose as substrates, D-sorbose and D-psicose can be synthesized by adopting D-glyceraldehyde and glucose as substrates, L-fructose can be synthesized by adopting L-glyceraldehyde and glucose as substrates, and 3R, 4S, 5R, 6R-heptose and 3R, 4R, 5R, 6R-heptose can be synthesized by adopting D-erythrose and glucose as substrates. Therefore, the corynebacterium glutamicum recombination strain SY10 disclosed by the invention can be applied to the field of production of the rare ketose and the deoxidized ketose by whole-cell fermentation; the produced rare ketose and deoxidized ketose have broad application prospects in the industries such as a food, a medicine and the like.

The invention discloses a Gluconobacter oxydans, which is classified and named as Gluconobacter oxydans NH-10, and preserved in China General Microbiological Culture Center of Microbial Culture Collection Management Committee with the preservation number of CGMCC No.2709. The invention further discloses a D-xylulose preparation method that uses the Gluconobacter oxydans. The strain obtained by screening can transform D-Arabitol so as to generate D-xylulose in high efficiency, the D-xylulose preparation method reaches the highest D-Arabitol transformation ratio of 99.5 percent (w / w) and the D-xylulose yield ratio of 95 percent, the D-xylulose concentration of a conversion fluid can reach 95g / L, and the conversion fluid hardly contains other ingredients.

The present invention relates to the use of nucleic acid molecules coding for a bacterial xylose isomerase (XI), preferably coming from Clostridium phytofermentans, for reaction / metabolization, particularly fermentation, of recombinant microorganisms of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol, by means of xylose fermenting yeasts. The present invention further relates to cells, particularly eukaryotic cells, which are transformed utilizing a nucleic acid expression construct which codes for a xylose isomerase, wherein the expression of the nucleic acid expression construct imparts to the cells the capability to directly isomerize xylose into xylulose. Said cells are preferably utilized for reaction / metabolization, particularly fermentation, of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol. The present invention also relates to methods for the production of bioethanol, and to methods for the production of further metabolization products, comprising the metabolization of media containing xylose.

Genetic recombinant yeast expressing xylose reductase (XR), (wild-type or mutant) xylitol dehydrogenase (XDH), and xylulokinase (XK) and a method for highly efficiently producing ethanol from xylose using the yeast are provided. Pichia stipitis-derived XR and (wild-type or modified-type) XDH genes and Saccharomyces cerevisiae-derived XK gene were introduced via chromosomal integration. Thus, a genetic recombinant yeast having a high xylose fermentation rate, being capable of producing ethanol from xylose in high yields, and having high xylose fermentability in the presence of glucose, as well as a method using the recombinant yeast for highly efficiently producing ethanol from xylose or a saccharified solution from lignocellulose-based biomass are provided. Furthermore, a method for improving the xylose fermentability of the genetic recombinant yeast of the present invention via acclimatization treatment is also provided herein.

The invention discloses a method for extraction of xylose isomerases by taking brown hot crack loving spore fungus as sources and the activity expression and application of the brown hot crack loving spore fungi xylose isomerases in Saccharomyces cerevisiae. The molecule weight of the brown hot crack loving spore fungi xylose isomerases is 43 kilodaltons; the most suitable reaction temperature of the xylose isomerases is 80 to 85 DEG C; and the most suitable reaction pH of the xylose isomerases is 7.0. The xylose isomerases can convert xyloses into xyluloses under normal biochemical reaction conditions; genes of the xylose isomerases can be recombined into the Saccharomyces cerevisiae in which the activity expression is realized, and the xyloses or other carbon sources such as lignocellulose hydrolysate and so on can be utilized for production of various fermentation products such as alcohols and so on. The method utilizes the genetic engineering means to expand substrates which are converted by the alcohols and has important theoretical significance and application value on full utilization of lignocellulose resources.

The present invention relates to microorganisms of corynebacterium which can utilize xylose and to a method for producing L-lysine using same. More particularly, the present invention relates to microorganisms of corynebacterium which are modified, in which genes encoding xylose isomerase and xylulokinase which are xylose synthases are introduced to express the xylose synthase. The present invention also relates to a method for producing L-lysine, comprising a step of culturing the modified microorganisms of corynebacterium using xylose as a carbon source, and recovering L-lysine from the culture.

The present invention is provides methods and formulations for preventing or ameliorating progression to chronic heart failure subsequent to cardiac stress, including as a consequence of myocardial infarction (MI), coronary artery disease, hypertension, cardiomyopathy, myocarditis, valvular regurgitation, severe lung disease, and / or severe anemia of chronic disease, by administration of one or more rate-limiting precursors to the synthesis of ATP. In one embodiment the ATP precursor is a pentose selected from one or more of ribose, D-ribose, ribulose, xylitol, xylulose, and a 5-carbon precursor of ribose.

The invention discloses a construction method of pediococcus acidilactici for producing L-lactic acid through co-fermentation of glucose and xylose, and belongs to the technical field of gene engineering. The construction method comprises the following construction steps: integrating heterogenous xylose isomerase, xylulokinase, transketolase and transaldolase coding genes on a genome of a strain pediococcus acidilactici TY112 (with the accession number of CGMCC NO.8664) for producing L-lactic acid by utilizing a thermosensitive knockout system; knocking out phosphoketolase and acetokinase coding genes; and carrying out adaptive evolution for improving the capacity of co-fermentation of glucose and xylose. With the construction method, an engineering strain capable of efficiently producingoptically pure L-lactic acid through co-fermentation of glucose and xylose is successfully obtained, the engineering strain is named P.acidilactici ZY271, and the accession number is CGMCC NO.13611.

The invention discovers that a xylose isomerase gene of non-glutinous rice has the function of converting xylose into xylulose. According to the invention, a recombinant plasmid containing the gene is constructed and is introduced into saccharomyces cerevisiae to obtain new engineered saccharomyces cerevisiae. Experiments prove that the saccharomyces cerevisiae which does not have the function ofconverting xylose into xylulose obtains the conversion capacity after the xylose isomerase gene is expressed in a host cell.

The invention discloses a rhodobacter sphaeroides engineering strain for producing beta-carotene and a construction method thereof. The method includes first optimizing a codon of a lycopene cyclase gene crtYPa derived from pantoea agglomerans to obtain an opt crtYPa gene, then replacing an endogenous phytoene three-step dehydrogenase gene crtI3 of rhodobacter sphaeroides with the opt crtYPa genecontaining a promoter prrnB and a phytoene four-step dehydrogenase gene crtIPa gene derived from the pantoea agglomerans in a traceless mode, then knocking out an endogenous neurosporene hydroxylase gene crtC of the rhodobacter sphaeroides and a 6-phosphoglucose dehydrogenase gene zwf and finally knocking out an endogenous 1-deoxyxylulose-5-phosphate synthase gene dxs of the rhodobacter sphaeroides with integration expression at zwf position to obtain the rhodobacter sphaeroides engineering strain for producing the beta-carotene. The strain is cultured by fermentation, and the content of the beta-carotene can reach 30mg / g DCW.

A method for producing a strain of Saccharomyces cerevisiae with introduced genes coding for xylose reductase, xylitol dehydrogenase and xylulokinase and with improved ethanol production, improved xylose conversion, reduced xylitol production and improved inhibitor tolerance is described. The method comprises culturing a strain of Saccharomyces cerevisiae at a continuous mode with a medium comprising essentially only xylose as carbon source at a temperature of 25-38° C., preferably 30-35° C., and an airflow of 0.040-0.055 vvm, and increasing the dilution rate to maintain a constant cell level, said cell level being in the range of 1.5-3.0 determined by optical density or equivalent analytical means, and adding at least one inhibitor to the cells and gradually increasing the addition of said inhibitor. Further, strains of Saccharomyces cerevisiae obtained by the method according to the invention are described.

The invention discloses a building method of pediococcus acidilactici ZP26 for producing D-lactic acid by co-fermenting glucose and xylose, and belongs to the field of gene engineering. The method comprises the following building steps of integrating heterogenous xylose isomerase, xylulokinase, transketolase and transaldolase encoding genes onto pediococcus acidilactici ZP26 (with the preservationnumber being CGMCC NO.8665) genomes by using a thermosensitive knock-out system; knocking out phosphotransferase encoding genes so as to block the generation of byproducts of acetic acids; the capability of the engineering strain for co-fermenting glucose and xylose is obviously improved through an adaptive domestication strategy. The method provided by the invention has the advantages that the building of the xylose metabolism path is successfully realized for the first time; the engineering strain for producing optical pure D-lactic acid by co-fermenting glucose and xylose at high efficiency is obtained, is named as P.acidilactici ZY15, and has the preservation number of CGMCC NO.13612.

Xylose-utilizing Zymomonas strains studied were found to accumulate ribulose when grown in xylose-containing media. Engineering these strains to increase ribose-5-phosphate isomerase activity led to reduced ribulose accumulation, improved growth, improved xylose utilization, and increased ethanol production.

The invention discloses a salvia 1-deoxidation xylulose-5-phosphat synthase gene1, protein which is encoded by the salvia 1-deoxidation xylulose-5-phosphat synthase gene1 and the use thereof, which fills a gap that the 1-deoxidation xylulose-5-phosphat synthase gene1 is separated and cloned from salvia which is precious traditional Chinese medicine in China. The 1-deoxidation xylulose-5-phosphat synthase gene1 which is provided by the invention has a nucleotide sequence or a homologous sequence which adds, replaces, inserts or losses one or a plurality of nucleotides or allele thereof and the nucleotide sequence which is derived from the 1-deoxidation xylulose-5-phosphat synthase gene1, which are displayed in the SED ID No.1. The protein which is encoded by the gene has an amino acid sequence or the homologous sequence which adds, replaces, inserts or losses one or a plurality of amino acids, which is displayed in the SEQ ID No.2. The 1-deoxidation xylulose-5-phosphat synthase gene1 which is provided by the invention has prominent effect of increasing the content of tanshinone in salvia through the technology of excessive expression of the gene and can be widely applied in improving the quality of salvia mi ltiorrhiza, which has very good application prospect.

The present invention provides for a genetically modified host cell capable of producing 1-deoxyxylulose 5-phosphate or 1-deoxy-D-xylulose 5-phosphate (DXP) (12), and optionally one or more DXP derived compounds, comprising: (a) a mutant RibB, or functional variant thereof, capable of catalyzing xylulose 5-phosphate and / or ribulose 5-phosphate to DXP, or (b) a YajO, or functional variant thereof, and a XylB, or functional variant thereof.

There is provided the use as reducing agents of alpha-hydroxycarbonyl compounds capable of forming cyclic dimers. There is also provided corresponding methods of reducing reducible compounds, particularly reduction-activated prodrugs . Examples of the alpha-hydroxycarbonyl compounds used are dihydroxyacetone, glycolaldehyde , glyceraldehyde, erythrose, xylulose, erythrulose or 3-hydroxy-2-butanone.

The invention relates to a method for selecting a 1-deoxy-D-xylulose-5-phosphate synthase inhibitor from a plant extract. The inbibitional effect of the added plant extract on phosphotriose isomerization activity of DXS enzyme is assessed by detecting the conversion quantity of a substrate with pre-column derivatization-high performance liquid chromatography is evaluated. The method mainly comprises the following steps of: adding the plant extract in a phosphotriose isomerization reaction system catalyzed by the DXS enzyme; after reaction at a certain temperature, hydrolyzing the phosphotriose in the reaction system with phosphatase; and next, adding 2,4-dinitrophenylhydrazine to the reaction system for derivatization and then performing detection by using an ultraviolet detector and taking methanol-water or acetonitrile-water as eluent gradients with high performance liquid chromatography. In the selection process of the method, the substrate D-glyceraldehyde 3-phosphoric acid (D-GAP) or dihydroxyacetone phosphate(DHAP) contains no isotope labeling; and the selection process is simple, quick and high in feasibility without any expensive large instrument for detection.

The invention discloses a red sage root 1-deoxy-xylulose-5-phosphate synthase II gene and its encoded proteins and applications, which fills the blank of separating the 1-deoxy-xylulose-5-phosphate synthase II gene from the traditional Chinese herb red sage root. The 1-deoxy-xylulose-5-phosphate synthase II gene provided by the present invention has the nucleotide sequence shown by the SEQ ID No.1 or homology sequence with one or a plurality of added, replaced, inserted or deleted nucleotide and its allelomorphic gene as well as it derived nucleotide sequence; the encoded protein has amino acid sequence shown in SEQ ID No.2 or r homology sequence with one or a plurality of added, replaced, inserted or deleted nucleotide. The 1-deoxy-xylulose-5-phosphate synthase II gene plays a significant role for improving the tanshinone content in the red sage root by a gene excess expression technique.

This disclosure generally relates to the use of enzyme combinations or recombinant microbes comprising same to make isoprenoid precursors, isoprenoids and derivatives thereof including prenylated aromatic compounds. Novel metabolic pathways exploiting Claisen, aldol, and acyioin condensations are used instead of the natural mevalonate (MVA) pathway or 1-deoxy-d-xylulose 5-phosphate (DXP) pathways for generating isoprenoid precursors such as isopentenyl pyrophosphate (IPP), dimethylallyl pyrophosphate (DMAPP), and geranyl pyrophosphate (GPP). These pathways have the potential for better carbon and or energy efficiency than native pathways. Both decarboxylative and non-carboxylative condensations are utilized, enabling product synthesis from a number of different starting compounds. These condensation reactions serve as a platform for the synthesis of isoprenoid precursors when utilized in combination with a variety of metabolic pathways and enzymes for carbon rearrangement and the addition / removal of functional groups. Isoprenoid alcohols are key intermediary products for the production of isoprenoid precursors in these novel synthetic metabolic pathways.

The invention discloses a corynebacterium glutamicum engineering strain for biosynthesis of rare sugar, and a building method and application thereof, and discloses a corynebacterium glutamicum recombination strain SY10. An experiment proves that rare ketose and deoxidized ketose can be synthesized by adopting a plurality of hydroxyaldehydes and glucoses as substrates by the strain in a fermentation manner, for example, D-erythrulose can be synthesized by adopting formaldehyde and glucose as substrates, L-xylulose can be synthesized by adopting glycolaldehyde and glucose as substrates, D-sorbose and D-psicose can be synthesized by adopting D-glyceraldehyde and glucose as substrates, L-fructose can be synthesized by adopting L-glyceraldehyde and glucose as substrates, and 3R, 4S, 5R, 6R-heptose and 3R, 4R, 5R, 6R-heptose can be synthesized by adopting D-erythrose and glucose as substrates. Therefore, the corynebacterium glutamicum recombination strain SY10 disclosed by the invention can be applied to the field of production of the rare ketose and the deoxidized ketose by whole-cell fermentation; the produced rare ketose and deoxidized ketose have broad application prospects in the industries such as a food, a medicine and the like.