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336 results about "Psicose" patented technology
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D-Psicose (C₆H₁₂O₆), also known as D-allulose, or simply allulose, is a low-calorie monosaccharide sugar used by some major commercial food and beverage manufacturers. First identified in wheat more than 70 years ago, allulose is naturally present in small quantities in certain foods.
Sweetener containing d-psicose and foods and drinks obtained by using the same
Providing a D-psicose-containing sweetener with the modification of the taste of D-psicose, comprising D-psicose, a sugar alcohol and / or a high intensity sweetener, preferably containing D-psicose as the main component, particularly a low-calorie sweetener and / or a sweetener giving refreshing feel in the oral cavity, as well as foods and drinks obtained by using the D-psicose-containing sweetener with the modification of the taste of D-psicose, and other products given with sweetness. The sugar alcohol is one or more sugar alcohols selected from the group consisting of sorbitol, mannitol, lactitol, maltitol, xylitol and erythritol, while the high intensity sweetener is one or more high intensity sweeteners as selected from aspartame, acesulfame K, sodium cyclamate, sodium saccharin, Sucralose (under trade name), stevia sweetener, dulcin, taumatin, neotame and monellin.
Owner:MATSUTANI CHEM INDS CO LTD +1
Complex crystalline sugar comprising d-psicose and d-allose and process for production of the same
ActiveUS20090068710A1Improve overall utilizationEfficient productionSugar derivativesSugar derivatives preparationAllosePsicose
Owner:KAGAWA UNIVERSITY +1
Novel sweetener having sucrose-like taste, method for producing the same, and use of the same
InactiveUS20100204346A1Great tasteLow costOrganic active ingredientsCosmetic preparationsSucroseIsomerase
Problem: To develop a method for producing a novel sweetener containing glucose, fructose, and psicose, which is produced from glucose liquid sugar using an isomerase and an epimerase; use of the novel sweetener as a food or drink material; and a novel sweetener capable of preventing obesity caused by the intake thereof.Means of Resolution: An isomerase and an epimerase are allowed to act on glucose liquid sugar produced in a glucose liquid sugar production plant to thereby produce D-psicose, thereby providing a novel sweetener (product) that maintains the degree and quality of sweetness of a glucose-fructose mixed solution and never causes obesity, a method for producing the same, and use of the same.
Owner:HAYASHIBARA CO LTD +3
Genetic engineering strain capable of effectively secreting D-psicose 3-epimerase and construction method and application thereof
ActiveCN105602879ASimple purification processLow production costBacteriaMicroorganism based processesPsicoseRumen
The invention belongs to the technical field of bioengineering, and particularly relates to a genetic engineering strain capable of effectively secreting D-psicose 3-epimerase and a construction method thereof. A D-psicose 3-epimerase gene rdpe from rumen bacterium Ruminococcus sp. 5_1_39B_FAA is obtained firstly, recombinant plasmid pMA5-RDPE construction and bacillus subtilis conversion are conducted, and then constitutive and secretive expression of RDPE in bacillus subtilis is achieved. By comparing three glucose-induced promoters, the optimal inducible promoter P[xylA] is obtained, and the RDPE secretion level is increased remarkably. By knocking out the xylose utilization gene xylAB (xylA and xylB), the xylose metabolism pathway of bacillus subtilis is blocked, the secretion amount of RDPE is further increased, and the optimal induced concentration of the inducer xylose is reduced to 0.5% from 4.0%. Finally, the engineered strain 1A751SD-XR is evaluated in a 7.5 L fermentation tank, and the RDPE secretion level can be as high as 95 U / mL and 2.6 g / L.
Owner:TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
Preparation method and application of allulose
ActiveCN104447888AIncrease profitSimple processSugar derivativesSugar derivatives preparationMolybdateDifferential phase
The invention provides a preparation method and application of allulose. The preparation method comprises the following step: by adopting glucose as a raw material and adopting molybdate as a catalyst, performing catalytic conversion, separation and purification to obtain allulose with corresponding configuration. The method provided by the invention mainly comprises the following step: by adopting a chemical method, preparing a crystal allulose product by virtue of working procedures such as chemical differential phase isomerism, refined chromatography separation and purification, concentration and crystallization and the like by adopting glucose as the raw material. The preparation method provided by the invention is simple in process, short in time consumption, high in reaction speed and high in efficiency, and the problems that a conventional enzymatic method for preparing D-allulose products is poor in enzyme stability and low in efficiency and the like can be solved.
Owner:SHANDONG FUTASTE
Immobilization of psicose-epimerase and a method of producing D-psicose using the same
The present invention relates to a method of successively producing D-psicose from D-fructose or D-glucose by using a psicose-epimerase derived from Agrobacterium tumefaciens which is expressed in a food safety form.
Owner:CJ CHEILJEDANG CORP
Method of utilizing physiological activity of rare saccharide and composition containing rare saccharide
A method of utilizing the physiological activity of a rare saccharide, wherein physiological-activity sensitive cells are treated with the rare saccharide to modify the function of the cells. A composition containing, as an active ingredient, a rare saccharide which is introduced into physiological-activity sensitive cells and has an effect of modifying the function of the cells. The cells are human cells. The composition is a functional food, a drug, or a cosmetic. The rare saccharide is a rare saccharide belonging to aldose and / or ketose. The aldose is D-allose, and the cells are selected from the group consisting of cancer-cell proliferation inhibitory activity sensitive cells and active-oxygen production inhibitory activity sensitive cells. The ketose is D-psicose, and the cells are selected from the group consisting of chemokine secretion inhibitory activity sensitive cells, microglia migration inhibitory activity sensitive cells, and hypoglycemic activity sensitive cells.
Owner:MATSUTANI CHEM INDS CO LTD +2
Noncarious material and anticarious agent containing rare sugar
Object: To provide a composition for preventing periodontal diseases (prophylactic agent of periodontal diseases), the composition having an excellent cariostatic property, being safe and stable for prolonged use and having less effects on flavor.Means for Resolution: A non-cariogenic material prepared by blending a rare sugar in the D form as selected from the group consisting of D-psicose, D-sorbose and D-tagatose, a rare sugar in the L form as selected from L-fructose, L-psicose and L-tagatose, or allitol as a rare sugar derivative, singly or in combination. A cariostatic agent comprising D-psicose, D-sorbose, D-tagatose, L-fructose, L-psicose and / or L-tagatose. A cariostatic agent in combination with catechins.
Owner:MATSUTANI CHEM INDS CO LTD +1
D-psicose 3-epimerase mutant with improved thermal stability, and continuous production of d-psicose using same
ActiveCN104160023AEfficient large-scale industrial preparationMaintain enzymatic activityRacemaces/epimerasesIsomerasesPsicoseMutant
The present invention relates to a D-psicose 3-epimerase mutant of which the thermal stability is improved by substituting an amino acid of a specific sequence number. In addition, the present invention relates to a recombinant vector comprising the gene of the D-psicose 3-epimerase mutant, and a recombinant strain transformed with the recombinant vector. Further, the present invention relates to an immobilized reactor prepared using the enzyme mutant or the recombinant vector, and a method for producing D-psicose using the immobilized reactor.
Owner:CJ CHEILJEDANG CORP
Utilization of Rare Sugars in Plant or Microorganism
InactiveUS20080182752A1Low toxicityEasy to degradePlant growth regulatorsBiocidePlant hormoneGrowth retardant
[OBJECT] To provide an agricultural chemical and the like with the use of an effect of inducing systemic acquired resistance in a plant. To provide a growth inhibitor of not only a plant pathogenic bacterium but also a harmful microorganism.[MEANS FOR RESOLUTION] Utilization of a rare sugar for inducing systemic acquired resistance in a plant or inhibiting the growth of a microorganism. Utilization thereof as an agricultural chemical with the use of the effect of inducing systemic acquired resistance in a plant, a plant disease inhibitor, an inducer of a plant growth regulatory factor (i.e., an inducer of plant hormone-like actions consisting of disease resistance, insect resistance, fruit maturation, breaking of dormancy, regulation of germination, drying resistance, and other than this, resistance to environmental stresses such as low temperature resistance, high temperature resistance, salt resistance and heavy metal resistance and promotion of flowering) and a microorganism growth inhibitor. The rare sugar is an aldose (D-allose, D-altrose or L-galactose) or a ketose (D-psicose or a mixture of D-psicose and D-fructose).
Owner:KAGAWA UNIVERSITY
Preparation method for high-purity D-psicose
ActiveCN106520746AImprove the ability to convert into D-psicoseEasy to controlSugar derivativesOrganic chemistry methodsChromatographic separationCentrifugation
Owner:SHANDONG BAILONG CHUANGYUAN BIO TECH
Immobilization of psicose-epimerase and a method of producing d-psicose using the same
The present invention relates to a method of successively producing D-psicose from D-fructose or D-glucose by using a psicose-epimerase derived from Agrobacterium tumefaciens which is expressed in a food safety form.
Owner:CJ CHEILJEDANG CORP
High-catalytic-activity mutant enzyme for D-allulose 3-epimerase and application thereof
ActiveCN103710330AHigh catalytic activityMicroorganism based processesOxidoreductasesMolecular biologyPsicose
The invention discloses a high-catalytic-activity mutant enzyme for D-allulose 3-epimerase and application thereof, belonging to the technical field of gene engineering of enzymes. The single mutant enzyme Y68I is obtained by taking D-sicose 3-epimerase (DPE enzyme for short) derived from clostridiumbolteae ATCC BAA-613 as a parent and replacing 68-bit tyrosine Tyr by isoleucine Ile with a gene mutation technology, is high in catalytic activity, and has an important industrial application value.
Owner:山东星光首创生物科技有限公司
Application of d-psicose to suppression of abnormal circadian increase in blood glucose level
ActiveUS20090062215A1Suppressing intra-day variationSuppressing abnormal increase of plasma glucose concentrationBiocideAntibiotics chemistryAcute hyperglycaemiaFood additive
A novel use of D-psicose (for suppressing the abnormal intra-day increase of blood glucose level) is provided. A composition containing D-psicose as the active component and for use in suppressing the abnormal intra-day increase of plasma glucose concentration. The composition is a composition in blend with D-psicose and / or a derivative thereof. The composition is in a form selected from sweeteners, seasonings, food additives, food materials, food and drink products, health food and drink products, pharmaceutical products, and feeds and blended with D-psicose, and / or a derivative thereof as the active component, for use in preventing and therapeutically treating diseases requiring the saving of insufficient insulin, the amelioration of insulin sensitivity and the amelioration of hyperglycemia, and / or diseases of which the symptoms can be ameliorated via the suppression of the abnormal intra-day increase of plasma glucose concentration or of which the onset can be prevented via the suppression thereof, such as diabetes mellitus, occult diabetic states, obesity, hyperglycemia, and / or arteriosclerosis. A method for using D-psicose in suppressing the abnormal intra-day increase of plasma glucose concentration, comprising giving D-psicose at given times to suppress the abnormal increase of plasma glucose concentration throughout the day.
Owner:MATSUTANI CHEM INDS CO LTD
Method for efficiently preparing D-psicose 3-epimerase and use of D-psicose 3-epimerase
InactiveCN107723307ALow costHigh catalytic efficiencyBacteriaMicroorganism based processesIsozymePsicose
The invention discloses a method for efficiently preparing D-psicose 3-epimerase and a use of the D-psicose 3-epimerase and provides a method for efficiently preparing an enzyme catalyst for catalyzing the same reaction through isozyme combined expression. The method utilizes corynebacterium glutamicum as a host cell to construct a recombinant strain expressed by plasmid dissociation and chromosomal integration, measures D-fructose catalytic efficiency, improves enzyme catalytic efficiency by 2-4 times than that of single expression of D-psicose 3-epimerase through combined expression and hasa conversion ratio of 29% when 70% of fructose is a substrate. The method improves D-psicose production efficiency and is suitable for industrial production of D-psicose. The invention also disclosesa novel use of the D-psicose 3-epimerase in psicose synthesis. The enzyme is derived from Paenibacillus senegalensis, has catalytic activity of about 25 U / mg and can be used to convert D-fructose intoD-psicose.
Owner:TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
68th and 109th double mutant enzyme of D-psicose 3-epimerase and application thereof
InactiveCN103849612AHigh catalytic activityFunction increaseMicroorganism based processesOxidoreductasesGenes mutationGenetics
The invention provides a 68th and 109th double mutant enzyme of D-psicose 3-epimerase and an application thereof, and belongs to the technical field of enzyme genetic engineering. The invention discloses the mutant enzyme Y68I / G109P which is obtained by using the D-psicose 3-epimerase (called DPE enzyme for short) derived from clostridium bolteae ATCCBAA-613 as a parent and utilizing a gene mutation technology for respectively replacing 68th tyrosine Tyr and 109th glycine Gly with isoleucine Ile and praline Pro. The heat stability and catalytic activity of the mutant enzyme Y68I / G109P are improved, therefore, the mutant enzyme Y68I / G109P has important industrial application value.
Owner:JIANGNAN UNIV
Application of L-rhamnulose-1-phosphate aldolase in catalytic synthesis of rare sugars
The invention discloses application of L-rhamnulose-1-phosphate aldolase in catalytic synthesis of rare sugars. In order to avoid the direct use of DHAP (dihydroxyacetone phosphate) and greatly decrease the synthesis cost of rare sugars, a 'single kettle with four enzymes' strategy is adopted, the L-rhamnulose-1-phosphate aldolase derived from Thermotoga maritima MSB8 is used for synthesizing a series of rare sugars such as D-allulose, D- sorbose, L-fructose and L-tagatose. Experiments show that the aldolase has good thermal stability and can be used for highly selectively synthesizing D-allulose having important application value while D-glyceraldehyde is used as a receptor.
Owner:JIANGNAN UNIV
D-psicose-3-epimerase mutant with improved catalytic activity and application of mutant
The invention provides a D-psicose-3-epimerase mutant with improved catalytic activity and an application of the mutant and particularly provides mutational D-psicose-3-epimerase. The mutational D-psicose-3-epimerase is obtained by mutation in at least three loci selected from the following groups: the 73rd aspartic acid (D), the 111st tyrosine (Y), the 188th asparaginate (N) and the 250th glycine(G) of wild D-psicose-3-epimerase corresponding to SEQ ID NO:1. The mutational D-psicose-3-epimerase has very high catalytic activity and catalytic efficiency (reaching up to 29.6%).
Owner:LIVINGZONE SHANGHAI BIO CHEM TECH CO LTD
D-psicose 3-epimerase mutant with improved thermal stability, and continuous production of d-psicose using same
ActiveUS20140199732A1Improve thermal stabilityEfficient productionBioreactor/fermenter combinationsBiological substance pretreatmentsWild typePsicose
The present invention relates to a D-psicose 3-epimerase variant with improved thermostability by substituting an amino acid at a specific position of an amino acid sequence of a wild type D-psicose 3-epimerase. Further, the present invention provides a recombinant expression vector including a gene of the D-psicose 3-epimerase variant, and a recombinant strain transformed with the recombinant expression vector. Furthermore, the present invention provides an immobilized reactor prepared using the D-psicose 3-epimerase variant or the recombinant strain, and a method of continuously producing D-psicose using the immobilized reactor.
Owner:CJ CHEILJEDANG CORP
Novel sweetener having sucrose-like taste, method for producing the same, and use of the same
To develop a method for producing a novel sweetener containing glucose, fructose, and psicose, which is produced from glucose liquid sugar using an isomerase and an epimerase; use of the novel sweetener as a food or drink material; and a novel sweetener capable of preventing obesity caused by the intake thereof. An isomerase and an epimerase are allowed to act on glucose liquid sugar produced in a glucose liquid sugar production plant to thereby produce D-psicose, thereby providing a novel sweetener (product) that maintains the degree and quality of sweetness of a glucose-fructose mixed solution and never causes obesity, a method for producing the same, and use of the same.
Owner:MATSUTANI CHEM INDS CO LTD +3
Preparation method of psicose crystals
ActiveCN110627847AHigh purityFine purity at high supersaturationSugar derivativesSugar derivatives preparationChromatographic separationEvaporation
The present invention relates to a preparation method of psicose crystals. The preparation method comprises the following steps: carrying out chromatographic separation of psicose liquid after an enzymatic conversion is finished and purifying the psicose to a purity of 98.5% or more; concentrating the purified psicose to a solid content by mass of 75-85% and conducting rapid cooling to 35-45 DEG C; adding seed crystals, keeping temperature at 35-45 DEG C and a vacuum degree of -0.03 to -0.09 MPa, and performing constant-temperature evaporative crystallization; and performing centrifugal separation to obtain crystals with a particle size larger than 60 meshes, and conducting washing and drying to obtain the psicose crystals. The technical means of controllable evaporation, constant-temperature crystallization, etc. are used to solve a problem that fine crystals are generated under a condition of high supersaturation degree, greatly improve particle diameter of the crystals, enable one-time crystallization yield to reach 60-70%, greatly simplify preparation processes and also improve crystallization yield.
Owner:SHANDONG BAILONG CHUANGYUAN BIO TECH
D-psicose 3-epimerase mutant with improved catalytic efficiency
ActiveCN108018278AHigh catalytic efficiencyIncreased relative enzyme activityBacteriaMicroorganism based processesPsicoseMutant enzyme
The invention discloses a D-psicose 3-epimerase mutant with improved catalytic efficiency and belongs to the technical field of enzyme engineering. The Dorea sp. DPEase mutant enzyme A38E / G105A keepsthe optimal catalytic conditions. Under the optimal catalytic conditions, the relative enzyme activity of the enzyme for catalytic conversion of D-fructose as a substrate into D-psicose is improved by38.6%. The discovery has an important research value for the industrial production of D-psicose.
Owner:JIANGNAN UNIV
Thermal stability improved mutant enzyme of D-psicose 3-epimerase and application thereof
InactiveCN103849613AImprove thermal stabilityMicroorganism based processesIsomerasesIsomerasePsicose
The invention provides thermal stability improved mutant enzyme of D-psicose 3-epimerase and application thereof, and belongs to the technical field of enzyme genetic engineering. The mutant enzyme is characterized in that D-psicose 3-epimerase (DPE enzyme for short) sourced from clostridium ((i) Clostridium bolteae ( / i)) ATCC BAA-613 is used as a parent, and glycine Gly at the position 109 is replaced by proline Pro through the gene mutagenesis technique so as to obtain single mutant enzyme G109P; the stability of the mutant enzyme is improved; high industrial application value is brought.
Owner:JIANGNAN UNIV
Method for preparation of co-expressed recombinase with genetic engineering technology
InactiveCN103710329AImprove thermal stabilityIncrease vitalityOxidoreductasesIsomerasesBiotechnologyNucleotide
The invention belongs to the technical field of co-expression of dienzyme, and in particular relates to co-expression of site-specific mutational glucose isomerase and D-allulose-3-epimerase. For overcoming the shortcomings of the prior art, the invention provides a method for performing site-specific mutagenesis to the 146th amino acid of the glucose isomerase and performing co-expression with the glucose isomerase and the D-allulose-3-epimerase. Specifically, the method comprises the following steps: respectively performing PCR (Polymerase Chain Reaction) amplification, purification and cloning to the nucleotide sequence of the glucose isomerase after site-specific mutagenesis and the nucleotide sequence of the D-allulose-3-epimerase; respectively digesting the cloned bienzyme nucleotide sequence with a pCDFDuet-1 carrier; purifying and connecting the digestion product to build a recombinant expression vector; inducing expression to obtain recombinant bacteria; collecting the recombinant bacteria and treating the recombinant bacteria by ultrasonication to obtain a crude enzyme liquid containing bienzyme.
Owner:SHANXI TIANJIAO FOOD +2
D-psicose 3-epimerase production strain and immobilization method thereof
ActiveCN108102995AGood continuous conversion abilityRealize continuous productionBacteriaMicroorganism based processesFructoseMicrobiology
The invention discloses a D-psicose 3-epimerase production strain and an immobilization method thereof, and belongs to the technical field of bioengineering. According to the invention, recombinant bacillus subtilis pHY300PLK-DPEase for producing D-psicose 3-epimerase is constructed, and the recombinant bacterium (the recombinant bacillus subtilis) serves as the production strain; an enzyme activity recovery rate of immobilized cells reaches 64%, the optimum temperature of the immobilized cells is improved by 5 DEG C in comparison with that of whole cells and the optimum pH value of the immobilized cells is free from obvious change; and after continuous conversion with 300g / L of fructose as a substrate for seven times, a conversion rate can be still kept at 28% and residual enzyme activitycan be still kept at 81%. The immobilization method is simple and easy to implement and immobilized particles are excellent in performance; therefore, a quite high industrial application value is achieved.
Owner:JIANGNAN UNIV
Novel D-allulose 3-epimerase and application thereof
ActiveCN109306347AImprove conversion rateImprove thermal stabilityBacteriaMicroorganism based processesBiotechnologyThermal stability
The invention belongs to the technical field of genetic engineering and in particular relates to novel D-allulose 3-epimerase, DNA (Deoxyribonucleic Acid) for encoding the novel D-allulose 3-epimerase, an expression vector and a transformant containing the DNA and application of the enzyme to production of D-allulose. The D-allulose 3-epimerase is derived from thermoacidophile mesoaciditoga lauensis of a deep-sea spring and has relatively high conversion rate and thermal stability when being used for converting D-fructose into the D-allulose.
Owner:JILIN COFCO BIOCHEM +1
Psicose epimerase and composition for conversion to psicose using same
A D-psicose 3-epimerization enzyme of the present invention is an enzyme with excellent activity for producing psicose by epimerizing a third carbon location of fructose. By applying the enzyme based on the wide pH range useful for industries and stability at high temperatures, the psicose with high economic feasibility can be mass-produced. The enzyme can be applied for producing functional saccharide, and for health food materials, medicines, and cosmetics using the same.
Owner:SAMSANG CORP
Allulose syrups
ActiveUS20180049458A1Broad customer appealQuality improvementFood ingredient as antioxidantSugar food ingredientsPsicoseFood products
Owner:TATE & LYLE SOLUTIONS USA LLC
Sweetener composition for alleviating diabetes, containing slowly digestible ingredient
InactiveUS20140342074A1Slowly digestibleAvoid symptomsOrganic active ingredientsSugar food ingredientsPsicoseDigestion
The present invention relates to a sweetener composition for alleviating diabetes, containing psicose and a slowly digestible or digestion-resistant polysaccharide as active ingredients, wherein diabetes alleviating effects and the quality of sweetness are enhanced.
Owner:CJ CHEILJEDANG CORP
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