41 results about "Transketolase" patented technology

The object is to develop a bacterium capable of fermenting glucose, mannose and xylose simultaneously, which can ferment a saccharified solution of a cellulose-type or lignocellulose-type biomass resource to produce ethanol, and to construct an energy-saving high-efficiency bioethanol conversion process. Thus, disclosed is Zymomonas mobilis bacterium which is prepared by integrating a gene encoding a phosphomannose isomerase derived from Escharichia coli into a levansucrase gene located on the chromosome by the double cross-over by means of a homologous recombsination method, and then introducing recombinant DNA prepared by binding a DNA fragment containing genes encoding a xylose isomerase, a xylulokinase, a transaldolase and a transketolase, respectively, all derived from Escherichia coli to a vector. Also disclosed is a method for producing ethanol by continuously fermenting a saccharified solution of a cellulose-type biomass resource in a system on which the Zymomonas mobilis bacterium is immobilized.

Process for the production of -lysine by constructing a recombinant microorganism which has a deregulated lysine 2,3-aminomutase gene and at least one deregulated gene selected from the group (i) which consists of aspartokinase, aspartatesemialdehyde dehydrogenase, dihydrodipicolinate synthase, dihydrodipicolinate reductase, tetrahydrodipicolinate succinylase, succinyl-amino-ketopimelate transaminase, succinyl-diamino-pimelate desuccinylase, diaminopimelate epimerase, diaminopimelate dehydrogenase, arginyl-tRNA synthetase, diaminopimelate decarboxylase, pyruvate carboxylase, phosphoenolpyruvate carboxylase, glucose-6-phosphate dehydrogenase, transketolase, transaldolase, 6-phosphogluconolactonase, fructose 1,6-biphosphatase, homoserine dehydrogenase, phophoenolpyruvate carboxykinase, succinyl-CoA synthetase, methylmalonyl-CoA mutase, provided that if aspartokinase is deregulated as gene (i) at least a second gene (i) other than aspartokinase has to be deregulated, and cultivating said microorganism.

The invention discloses recombinant corynebacterium glutamicum capable of being used for highly yielding L-phenylalanine and a method for producing the L-phenylalanine (Phe) by fermentation and belongs to the field of metabolic engineering. According to the invention, a corynebacterium glutamicum engineering strain C.glutamicum19AF / 99TP capable of being used for highly yielding the L-Phe is obtained in a corynebacterium glutamicum type strain ATCC13032 by carrying out induction expression on the following four genes: a 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase gene, a chorismate mutase / prephenate dehydratase gene which has resistance to feedback inhibition, a transketolase gene and a phosphoenolpyruvic acid synthetase gene. A shikimic acid metabolic pathway of an L-Phe synthetic metabolic pathway is over-expressed in the C.glutamicum ATCC13032; a key enzyme gene of a chorismic acid metabolic pathway enables the yield of the L-Phe to reach 3.47g / L; two enzyme genes which combines an expression center metabolic pathway to improve a precursor enables the highest yield of the L-Phe to reach 4.86g / L; and the original strain ATCC13032 which is used as a contrast strain cannot detect accumulation of the L-Phe in the integral fermenting process.

The invention relates to a preparation method of 4-phosphoric acid erythrose, comprising the following steps: immobilizing heat-resistant transketolase at the temperature of 55-65 DEG C; and then taking 6-fructose phosphate and 3-glyceraldehyde phosphate as raw materials to prepare the 4-phosphoric acid erythrose by enzymatic reaction. In the preparation method, the heat-resistant and specifically transformed transketolase of the 4-phosphoric acid erythrose is prepared by adopting a molecular biology technology, and the 6-fructose phosphate and the 3-glyceraldehyde phosphate are taken as the raw materials to efficiently prepare the 4-phosphoric acid erythrose by adopting an immobilized enzyme technology. The preparation method is simple and practical.

The invention discloses a recombinant Saccharomyces cerevisiae strain and a construction method for producing dammarenediol and protopanaxadiol by using xylose. Through homologous recombination, the promoter of Saccharomyces cerevisiae xylulokinase gene XKS1 is replaced with a promoter Sub P FBA1 , and then introduce xylose reductase XYL1, xylitol dehydrogenase XYL2 expression cassettes, and then increase the activity of transketolase TKL1 and transaldolase TAL1 to obtain recombinant bacterium 1, and then introduce farnesyl diphosphate into recombinant bacterium 1 Farnesyltransferase gene ERG9, squalene monooxygenase gene ERG1 and dammarene diol synthase gene DS were obtained to obtain recombinant bacteria 2, and nicotinamide adenine dinucleotide-hydroxymethyl was introduced into recombinant bacteria 2 Glutaryl-CoA reductase gene NADH-HMGr, farnesyl pyrophosphate synthase ERG20, protopanaxadiol synthase-cytochrome P450 reductase fusion protein gene PPDS-ATR1 were obtained to obtain recombinant bacteria 3, the recombinant brewing wine of the present invention Yeast uses xylose to synthesize dammarenediol and protopanaxadiol.