181results about How to "Improve fermentation performance" patented technology

The invention discloses a preparation method of a methane dry fermentation compound bacterial preparation, which comprises the following steps: mixing fermentation substrate with an inoculum which is compound bacteria composed of cellulose decomposing bacteria, proteolytic bacteria, fat decomposing bacteria, hydrogen-producing aceogenic bacteria, sulfate reducing bacteria and methanogenic archaea, adjusting the water content and pH of the mixed materials to 70-80% and 7.0 respectively, performing anaerobic fermentation under the condition of 35 DEG C plus or minus 2 DEG C for 60-90 days to obtain methane dry fermentation compound bacterial preparation. The preparation method of the invention can prepare compound bacterial preparation with a relatively complete methane fermentation function, and the total number of microbial cells and methanogenic archaea cells in the bacterial preparation are at least 10*10<10> / g and 1.0*10<8> / g respectively. Using the compound bacterial preparation as an inoculum of methane dry fermentation can reduce the starting time, and the fermentation process is stable and easy to control.

The invention provides a resource utilization method for the combined production of a biogas and a cultivation matrix through utilizing tobacco stalk fermentation. The method comprises the following steps: carrying out anaerobic fermentation of a main fermentation raw material which is waste tobacco stalks generated in the tobacco threshing redrying line to produce the biogas; carrying out solid-liquid separation of a biogas slurry and the tobacco stalks to obtain a tobacco stalk biogas residue and a biogas slurry; purifying the biogas to prepare clean fuels or to be used for electricity generation to provide partial energy for a plant area; and decomposing the tobacco stalk biogas residue, and carrying out dry molding to prepare the cultivation matrix, wherein the biogas slurry returns to a fermentation system to be recycled or to be used as a liquid organic fertilizer. The method provided by the invention has the advantages of simple production operation, concentrated and stable raw material, scale production benefiting, low production cost, realization of no pollution, zero discharge and cycle utilization of waste tobacco stalk resources of a threshing redrying plant, and generation of good economic, ecological and social benefits.

The invention relates to a low-yield high-grade alcohol high-yield ethyl lactate saccharomyces cerevisiae strain and a construction method thereof. The method comprises acquisition of a target gene, construction of a chromosome integrated vector and fermented verification of the breeding bacterial strain. The selected bacterial strain performs heterogenous secrete expression on pseudonym antarctica lipase B, compared with parent strains, the fermentation performance of the constructed saccharomyces cerevisiae is not influenced, after fermentation by sorghum raw material semi-solid liquor for 6 days, ethyl lactate content of the breeding bacterial strain is increased by 57.4%-84.5%; the high-grade alcohol production amount is obviously reduced, isoamyl alcohol is reduced by 9.5%, isobutyl alcohol is reduced by about 50.0%, and active-amyl alcohol is reduced by about 44.0%. According to the breeding bacterial strain, the production amount of ethyl lactate is obviously increased, the high-grade alcohol production amount is obviously reduced, the problem of incompatibility of flavor of the wind can be solved at certain degree, and the method has wide application prospect in the wine-related filed.

The invention discloses a high temperature resistant oil-in-water drilling fluid, which is realized through the following technical scheme of: (1) by mass, mixing 3.0%-4.0% of sodium bentonite, 0.2%-0.5% of NaOH, 0.1%-0.3% of a temperature and salt resistant filtrate reducer A, 0.2%-0.5% of a carboxymethylcellulose sodium salt, 2.0%-3.0% of sulfonated phenolic resin, 0.8%-2.0% of sulfonated lignite, 0.05%-0.15% of a high temperature stabilizer B, 1.0%-3.0% of an anti-high temperature emulsifier C, 3%-7% of KCl, 2%-5% of asphalt, and the balance water to obtain an aqueous phase; (2) mixing the aqueous phase obtained in step (1) with diesel oil or white oil according to a volume percentage of 7:3, 6:4, 5:5, 4:6 and 3:7 and performing emulsification, and then carrying out high temperature aging to obtain the high temperature resistant oil-in-water drilling fluid. The formed high temperature resistant oil-in-water drilling fluid has temperature resistance up to 180DEG C and a lowest density of 0.88g / cm<3>, can effectively prevent formation leakage of low pressure depleted oil and gas zones, is conducive to enhancement of the productivity of oil-gas wells, and is suitable for the technical field of petroleum drilling and development of low pressure oil and gas reservoirs.

The invention discloses a strain for preventing and treating metabolic diseases and application for the strain. The strain is Akkermansia muiniphila strain which is named Akkermansia muciniphila SSYD-3 and has the preservation number being CGMCC No.14764. Compared with the existing Ackermann muiniphila strain ATCC BAA 835, the Akkermansia muiniphila strain provided by the invention is more ideal in fermentation performance, storage stability, acid resistance, bile salt tolerance and the effect of treating the metabolic diseases.

The invention discloses saccharomyces cerevisiae CGMCC No.2927 for producing abricotine, a preparation method for fermenting the abricotine by using saccharomyces cerevisiae strains, and the brewed abricotine. By separating and purifying a plurality of strains of saccharomyces by different paths and alcohol, SO2, high-low temperature, high-sugar tolerance experiment and abricotine fermentation experiment, a strain of abricotine saccharomyces with good brewing performance is screened; and an abricotine fermentation process is determined, and then the abricotine with golden and clear color, intense fruit taste and wine taste, and full and comfortable mouth feel is brewed. The preparation method is widely applied in the field of light industrial production, and the like.

The invention relates to a micro-crosslinking polymer fluid loss additive for drilling fluid and a preparation method of the micro-crosslinking polymer fluid loss additive and belongs to the technical field of a preparation method of chemical high-molecular polymers of oil drilling fields. The micro-crosslinking polymer fluid loss additive is prepared from an organic acid monomer A with carboxyl, acrylamide, a monomer B for inhibiting acylamino hydrolysis, a temperature-resistant monomer C with a large side group, and a crosslinking agent by adopting aqueous solution copolymerization reaction under the initiation of an oxidation-reduction initiator system; and the obtained gel product is pelletized, baked and crushed, so as to obtain the micro-crosslinking polymer fluid loss additive for drilling fluid. The micro-crosslinking polymer fluid loss additive disclosed by the invention has good high-temperature fluid loss resistance; after the micro-crosslinking polymer fluid loss additive is added to 4% base slurry and is aged for 16 hours at 270 DEG C, the filter loss is reduced along with the increase of the micro-crosslinking polymer fluid loss additive disclosed by the invention; and when the addition amount of the micro-crosslinking polymer fluid loss additive is 0.01g / mL, the filter loss is reduced to 8.2mL from 46.0mL so that good performance of the drilling fluid is kept at 270 DEG C.

The invention discloses a high-temperature-resistant high-yield cellulase bacillus subtilis and application thereof. A cellulase production strain is separated from surface soil of a corn straw pile in a Jiangjin area of Chongqing, and the preservation number of the bacillus subtilis is CCTCC NO: M 2020002; a strain Z2 with the best enzyme production performance is finally obtained through enrichment culture, a CMC-Na plate experiment, a lignin plate experiment and an enzyme production experiment, and the obtained strain is subjected to 16S rRNA sequence comparison and identification to determine that the strain is bacillus subtilis Z2. By optimizing the culture medium and the culture conditions, the filter paper enzyme activity, the CMC enzyme activity and the beta-glucosidase activity under the optimal enzyme production conditions of 0.800 U / ml, 5.20 U / ml and 2.07 U / ml are obtained respectively. Cellulase produced by the strain has relatively high activity and stability under the conditions of a buffer solution with the pH value ranging from 4.0 to 10.0 and the temperature of 30 DEG C and 80 DEG C. Cellulase produced by the strain is mixed with commercial xylanase, then a saccharification experiment is carried out on pretreated straw stalks, and 84.27 mg / ml of total reducing sugar can be obtained.

The invention discloses a preparation method of a filtrate reducer for drilling fluid.The preparation method of the filtrate reducer for the drilling fluid is characterized by comprising the following steps: 1, dissolving 2.5-3.0 parts by weight of an alkaline substance A and 0.6-0.75 part by weight of metallic oxides in 100 parts by weight of water, and stirring until the alkaline substance and the metallic oxides are dissolved fully to obtain a solution a; b, adding 9-11 parts by weight of monomer C in the solution a, and stirring until the monomer C is dissolved fully to obtain a solution b; c, adding 18-22 parts by weight of monomer D into the solution, and stirring until the monomer D is dissolved fully to obtain a solution c; d, adding 0.9-1.0 part by weight of monomer E into the solution c, and stirring until the monomer E is dissolved fully to obtain a solution d; e, continuing stirring, and heating the solution d in water bath until the temperature reaches 70+ / - 5 DEG C; and f, maintaining the temperature to be 70+ / - 5 DEG C, slowly dropwise adding 0.08-0.1 part by weight of an initiator F into the solution d while stirring, and reacting for 4-5 hours to obtain a preliminary product.The filtrate reducer prepared by the method seldom affects the performance of the water-based drilling fluid, and resistance of the water-based drilling fluid to ultra-high calcium pollution can be enhanced effectively.

The invention relates to a method for producing erythritol by using broken rice. In the method, moniliellaacetoabutans (ATCC18455) is used as a production strain and broken rice sugar liquid is used as a fermentation substrate for preparing the high-purity erythritol; and the method comprises three steps, namely preparation of the broken rice sugar liquid, preparation of erythritol fermentation liquid and refining of an erythritol product. The erythritol has high transformation rate, short fermentation period and stable fermentation performance, and has no byproducts such as glycerol and the like; the method reduces the production cost, and has the advantages of low operating cost, small equipment space, high product yield, few impurities and simple process; and the purity of the final product reaches over 98 percent.

The invention discloses a method for screening cellulase producing strains and a method for producing cellulase by means of fermentation. The cellulase producing strains which are named as TP-02 are acquired from rotten wood in ecological forest in the Huangshang Mountain by means of primary screening, protoplast preparation, ultraviolet-irradiation mutation breeding and secondary screening. The methods have the advantages that the cellulase producing strains are high in cellulase producing capacity, bean pulp, ammonium sulfate, urea or ammonium chloride can be used as nitrogen sources, bran juice, lactose, starch sugar, rice straw or corn straw can be used as carbon sources, the nitrogen sources and the carbon sources are subjected to liquid-state fermentation to obtain the cellulase producing strains, the enzyme activity of filter paper can reach 16.98 IU / mL after the cellulase producing strains are cultivated for 72-84 hours, the enzyme activity of incision enzymes can reach 41.33 IU / mL, the enzyme activity of excision enzymes can reach 3.65 IU / mL, and the enzyme activity of beta-glucanase can reach 15.98 IU / mL.

The invention relates to a selectively hydrogenated isoprene-styrene diblock copolymer having a polystyrene content of at least 40 wt. %, which when added to an oil-based mud composition, substantially reduces fluid loss. The polystyrene in the diblock has a true molecular weight greater than 60 kg / mole and the total apparent molecular weight of the diblock copolymer is at least 160 kg / mole. The drilling mud that contains about 0.5 pounds per barrel to about 10 pounds per barrel of a selectively hydrogenated isoprene-styrene diblock copolymer and a weighting agent, resulting in a drilling fluid composition exhibiting good suspension of the weighting agent and improved fluid loss performance.

The object is to develop a bacterium capable of fermenting glucose, mannose and xylose simultaneously, which can ferment a saccharified solution of a cellulose-type or lignocellulose-type biomass resource to produce ethanol, and to construct an energy-saving high-efficiency bioethanol conversion process. Thus, disclosed is Zymomonas mobilis bacterium which is prepared by integrating a gene encoding a phosphomannose isomerase derived from Escharichia coli into a levansucrase gene located on the chromosome by the double cross-over by means of a homologous recombsination method, and then introducing recombinant DNA prepared by binding a DNA fragment containing genes encoding a xylose isomerase, a xylulokinase, a transaldolase and a transketolase, respectively, all derived from Escherichia coli to a vector. Also disclosed is a method for producing ethanol by continuously fermenting a saccharified solution of a cellulose-type biomass resource in a system on which the Zymomonas mobilis bacterium is immobilized.

The invention relates to a high-density low-frictional-resistance drilling fluid, and belongs to the technical field of oil-field drilling fluids. The high-density low-frictional-resistance drilling fluid employs water as a base liquid, and is obtained by mixing 1%-3% by mass of sodium bentonite, sodium carbonate accounting for 5% by mass of sodium bentonite, 0.5%-1.0% by mass of sodium hydroxide, 0.05%-0.1% by mass of tackifier A, 0.2%-0.5% by mass of filtrate reducer B, 0.5%-1.0% by mass of carboxymethyl cellulose sodium salt, 3.0%-5.0% by mass of sulfonated phenolic resin, 3.0%-5.0% by mass of sulfonated lignite, 2.0%-3.0% by mass of polymeric alcohol, 100% by mass of an organic salt C and 29.1%-122.2% by mass of barite, and performing high-temperature ageing. The drilling fluid has the temperature resistance up to 135 DEG C and the controllable density scope of 1.50-1.80 g / cm<3>, has good rheological property and filtration property at a high temperature, and has relatively low barite content, mud cake sticking coefficient and cyclic pressure dissipation, and relatively high inhibition performance.