3189 results about "Bioengineerings" patented technology

Novel mixtures of sugar amides or sugar amines are disclosed that have improved thermal properties over the individual components. New feedstocks based on both the surfactant tail as well as the sugar head group allow for improved physical properties of sugar amide surfactant mixtures and thus improved formulatability. Furthermore, new sources of unique methyl esters from both bioengineering and or co-metathesis of fats and oils provide novel and improved sugar amide surfactant mixtures.

A preferred embodiment of the present invention comprises computer-implemented methods for providing user assistance in biomachine design that, first, retrieve one or more digitally-represented candidate design items stored in a bioengineering knowledge base by translating requirements provided for a biomachine according to a bioengineering domain model into queries to the knowledge base for design items capable of implementing the biomachine according to the domain model; then second, construct one or more digitally-represented candidate biomachines from the candidate design items by arranging part information represented in the candidate design items according to a selected structure, and next evaluate the candidate biomachines according to bioengineering operability knowledge associated with the candidate design items, wherein operability knowledge associated with a design item specifies requirements for that item to inter-operate with other design items. The methods may backtrack. If at least one candidate biomachine has not been satisfactorily evaluated, the methods backtracking to one or more of these steps. The invention further encompasses variations of these methods, systems and program products performing these methods, data products including digital representations of design knowledge used by these methods, data products with digital representations of designed biomachines. Also encompassed are further steps of constructing or synthesizing biomachines along with the actual biomachines themselves.

The invention belongs to the field of bioengineering and computers, relating to a phase characteristic extraction method for brain waves of compound imaginary movements of lower limbs. The method comprises the following steps of: 1, collecting and preprocessing signals of brain waves of compound imaginary movements of lower limbs; 2, decomposing oscillation mode of brain waves of compound imaginary movements of lower limbs; 3, identifying characteristic oscillation mode; 4, extracting and identifying coordinate characteristic in functional areas; and 5, identifying mode identification. The method takes the unstability of the brain wave signals into consideration adequately, and has the maximum rate of identification of 87.8% which is obviously enhanced compared with 82.3% of the traditional method.

A process for purifying 1,3-propanediol from the fermentation broth of a cultured E. coli that has been bioengineered to synthesize 1,3-propanediol from sugar is provided. The basic process entails filtration, ion exchange and distillation of the fermentation broth product stream, preferably including chemical reduction of the product during the distillation procedure. Also provided are highly purified compositions of 1,3-propanediol.

The present disclosure relates to bioengineering approaches for producing biofuel and, in particular, to the use of a C1 metabolizing microorganism reactor system for converting C1 substrates, such as methane or methanol, into biomass and subsequently into biofuels, bioplastics, or the like.

The invention belongs to the technical field of bioengineering, and particularly relates to a genetic engineering strain capable of effectively secreting D-psicose 3-epimerase and a construction method thereof. A D-psicose 3-epimerase gene rdpe from rumen bacterium Ruminococcus sp. 5_1_39B_FAA is obtained firstly, recombinant plasmid pMA5-RDPE construction and bacillus subtilis conversion are conducted, and then constitutive and secretive expression of RDPE in bacillus subtilis is achieved. By comparing three glucose-induced promoters, the optimal inducible promoter P[xylA] is obtained, and the RDPE secretion level is increased remarkably. By knocking out the xylose utilization gene xylAB (xylA and xylB), the xylose metabolism pathway of bacillus subtilis is blocked, the secretion amount of RDPE is further increased, and the optimal induced concentration of the inducer xylose is reduced to 0.5% from 4.0%. Finally, the engineered strain 1A751SD-XR is evaluated in a 7.5 L fermentation tank, and the RDPE secretion level can be as high as 95 U / mL and 2.6 g / L.

The invention discloses a method for producing Anrodia camphorata mycelia based on solid-state surface culture, and belongs to the field of bioengineering. The method comprises the following steps of: screening components of a culture medium of the Anrodia camphorate and optimizing the proportion of the cereal raw material in the culture medium so that the culture medium is firmly adsorbed to the inner wall of a culture vessel or the culture surface to bring convenience to the surface culture of the Anrodia camphorata mycelia; inoculating the Anrodia camphorata mycelia in the culture surface of a reactor and culturing the Anrodia camphorata mycelia in the environment of certain temperature and moisture; feeding the culture medium every a certain time in batch during the surface culture so as to timely replenish the nutrient components required by the growth of the Anrodia camphorata mycelia and making the growth environment of the Anrodia camphorata mycelia close to the natural culture environment until a large amount of mycelia or large colony is formed; and quickly drying the Anrodia camphorata culture at a low temperature after the culture. The method of the invention makes the growth environment of the Anrodia camphorata mycelia close to that of the wild Anrodia camphorate and improves the productivity of the Anrodia camphorata culture by timely feeding the culture medium in batch, so that the obtained Anrodia camphorata culture has high content of active ingredient and closer efficacy to the wild fruit body.

The invention relates to the field of bioengineering technology, and in particular to a method for in-vitro induction of cholangiocyte-like transformation of primary hepatocytes and for long-term culture, amplification and differentiation and an application of the method. The invention provides a hepatocyte cholangiocyte-like transformation medium determined by chemical ingredients and / or a system which is composed of a hepatocyte mature medium and is applicable to long-term stable culture, amplification and differentiation of the primary hepatocytes; the invention also provides the method for in-vitro induction of cholangiocyte-like transformation of the primary hepatocytes and for long-term culture, amplification and differentiation; and with the application of the method, the cholangiocyte-like hepatocyte conversion of the primary hepatocytes can be induced in vitro, so that the obtained hepatocytes have characteristics of biliary epithelial cells and hepatic precursor cells, and the hepatocytes are applicable to long-term stable culture and amplification. The breedable cholangiocyte-like hepatocytes and hepatocytes, which are mature in differentiation, prepared by the invention are applicable to such aspects as toxicologic and pharmacological evaluation of compounds and drugs, researches and diagnosis & treatment of hepatitis viruses, treatment by hepatocyte transplantation, preparation of bioartificial liver and the like.