75 results about "Phosphatidase" patented technology

The invention relates to a method for preparing phosphatidylserine abundant in polyunsaturated fatty acid and belongs to the technical field of bioengineering. The method is characterized by comprising the following steps of: firstly, catalyzing ester exchange reaction between phosphatide and polyunsaturated fatty acid ester by utilizing one or a mixture of phosphatidase A and lipase to generate phosphatide abundant in polyunsaturated fatty acid; and then catalyzing phosphor-transfer esterification reaction between the phosphatide abundant in the polyunsaturated fatty acid and L-serine by utilizing phosphatidase D to generate the phosphatidylserine abundant in the polyunsaturated fatty acid. The method has the advantages of no discharge of waste water, good product quality, no solvent residue, safe process operation, few reaction byproducts, no waste generation, cost reduction, simple production process and easy realization of scale production because of utilizing two enzymes to perform sub-step catalysis and perform reaction in the same reactor, and completing the reaction process in a non-solvent system. Therefore, the invention provides a good and feasible method for preparing the phosphatidylserine abundant in the polyunsaturated fatty acid.

The invention relates to a method for digesting grease through microwave pretreatment and an aqueous enzymatic method. The method includes soaking raw materials, performing microwave puffing treatment on camellia oleifera seed slurry, digesting grease with water, performing physical refining on the obtained grease after degumming through a phosphatidase enzymatic method, or directly performing rough filtration, refined filtration and nanofiltration, and then performing vacuum dehydration and drying to obtain grease products and a by-product of wet meal. According to the method, the microwave puffing treatment is adopted on raw materials such as camellia oleifera seeds, cell structures of the raw materials can be fully destroyed, and the oil yield of the following water immersion method can be improved; grease digestion through water after microwave pretreatment for materials belongs to an environment-friendly and safe production process; and the method has the advantages of being simple and environment-friendly in process, high in oil yield, low in investment and prone to industrial production.

The invention discloses a method for degumming vegetable fat by using phosphatidase A2. The method comprises the following steps of: A, heating crude oil to 40-50 DEG C; B, adding a 45 percent (W/W) citric acid solution till the final concentration is 0.01-0.02 percent, stirring (at the speed of more than or equal to 40 revolutions per minute) or homogenizing for 10 seconds, and preserving heat for 30 minutes; C, adding water in an amount of 1-5 percent based on the weight of the crude oil, and stirring uniformly; and D, adding enzyme in an amount of 0.005-0.015 percent (W/W) based on the weight of the crude oil, stirring and reacting for 1-3 hours, and centrifuging or naturally precipitating for separating degummed oil from a gum deposit. The method is practicable, and is easy and convenient to operate; when phosphatidase A2 is used for degumming, high-value enzyme hydrolyzed phospholipid can be obtained, so that energy consumption is lowered; and expressed (or leached) crude oil with very high phosphorus content or hydrated degummed oil with relatively low phosphorus content can be directly used for degumming in the method, so that the method has a wider application range.

This invention open a cold tolerance of Serratia sand Habitat-chuen (Serratia fonticola) xjF1 CGMCC No.1971, through the strain characteristics, enzyme production conditions and the nature of enzyme research, proved a new phospholipase A1 gene consists of two open plA and plS reading frame, was certified plA gene coding phospholipase A1, plS gene coding phospholipase A1 auxiliary protein. This invention relates to the gene containing the recombinant plasmid and reorganization cell, the gene expression products phospholipase A1 in the show activity under low-temperature conditions, can be used in industries such as oil refining process can be simplified production process, lowering energy consumption, improve product quality , protecting the environment, reduce production costs, have important practical significance.

The invention relates to an acid soil amendment and its preparation method. the soil amendment contains the following ingredients (by weight): 4-12 parts of silicon dioxide, 1-6 parts of calcium oxide, 3-10 parts of biochar, 3-8 parts of lignin, 2-6 parts of urea, 2-6 parts of sodium silicoaluminate, 1-4 parts of organic bentonite, 0.3-0.7 part of polymaleic anhydride, 0.5-1 part of hydrogen peroxide, 0.3-0.7 part of beta-cyclodextrin, 0.4-1 part of phosphatidase, 0.2-0.8 part of powder of Chinese prickly ash and 0.2-0.8 part of sweet potato powder. The soil amendment provided by the invention can effectively raise pH value of acid soil and improve the defect of short period of soil. Meanwhile, the acid soil amendment can effectively kill noxious bacteria in soil and also can effectively reduce content of heavy metal ion in soil.

The invention provides a method for immobilizing phosphatidase A1. The method is improved on the technical basis of cross-linked enzyme aggregates and comprises the steps of: preparing free phosphatidase A1 into cross-linked phosphatidase aggregates; and then entrapping the obtained cross-linked phosphatidase aggregates to obtain immobilized phosphatidase A1. The influences of concentration of a phosphatidase liquid, saturation degree of a precipitating agent, precipitation Ph value, precipitation time, concentration of a cross-linking agent, cross-linking time, concentration of sodium alginate, concentration of calcium ions and other different immobilizing conditions on the immobilizing efficiency and the catalytic effect of the phosphatidase are researched, and the enzymology nature of an immobilized phosphatidase membrane is discussed. The advantage of immobilizing the phosphatidase by adopting the method for entrapping and cross-linking aggregates is further explained through an SEM (Scanning Electron Microscope) image of the immobilized phosphatidase membrane. The phosphatidase is immobilized by utilizing the excellent chemical stability, mechanical property and other properties of the entrapped and cross-linked phosphatidase aggregates, the activity and the stability of the phosphatidase in a reaction system are improved, the activity and the selectivity of the phosphatidase are adjusted and controlled, so as to be favorable for the recovery of the phosphatidase and the maintenance of higher activity recovery rate of the phosphatidase; and the activity recovery rate of the finally obtained immobilized phosphatidase is 80.2 percent, and the relative activity of the phosphatidase after being repeatedly used for seven times is kept at 65 percent above.

The invention relates to a non-bitter fishy smell, instant whole egg powder and a preparation method thereof. The method comprises: (a) adopting two-stage dynamic high-pressure micro-jet treatment; (b) sequentially adding compound protease and phospholipid to the whole egg liquid Enzyme A1; wherein the composite protease is papain and flavor protease, the dosage ratio of the two is 2:1, the amount of composite protease added in the whole egg liquid is whole egg liquid: composite protease=100:0.8-1.2, add The amount is calculated by mass ratio; phospholipase A1 is enzymatically hydrolyzed at room temperature, and the added amount in the whole egg liquid is whole egg liquid: phospholipase A1=100:0.01‑0.05, and the added amount is calculated by kg/L; (c) After spray drying, whole egg powder is fluidized bed granulated with a binder. The invention cooperates with dynamic high-pressure micro-jet homogenization, enzymatic hydrolysis and fluidized bed technology to obtain whole egg powder with good instant solubility, and no stratification is found within 10 hours after brewing.

The invention relates to a phosphatidase A1 immobilization method. The method comprises the following steps: 1, allowing a carrier selected from macroporous adsorption resin or ion exchange resin to contact with phospholipase A1 in order to immobilize the phospholipase A1 on the carrier; and 2, drying the phospholipase A1 immobilized carrier obtained in step 1 in order to obtain immobilized phospholipase A1. The invention also provides immobilized phospholipase A1 prepared through the method, and an application of the immobilized phospholipase A1. The immobilized phospholipase A1 has the functions of esterification and acid hydrolysis, can be repeatedly used in the oil industry, and has the advantages of high reaction efficiency low cost and potential industrial application.

The invention belongs to the technical field of genetic engineering of enzymes, and particularly relates to a method for obtaining phosphatidase B mutants with improved enzyme activity through a PCR (sequential error-prone) technology in vitro directed evolution, using the phosphatidase B mutants to prepare glycerophosphatidylcholine and performing oil degumming. The phosphatidase B mutant PLBM is obtained; the enzyme activity is improved by 25 percent through being compared with that of wild phosphatidase B.

The invention discloses a purpose of phosphatidase Dzeta2 protein on the aspect of adjusting the sensibility of plants to somatotropic hormone or the response aspect to the low phosphorus stress. The invention also discloses a preparation method for the plants which have high sensibility to the somatotropic hormone or high responsibility to the low phosphorus stress. The invention leads the phosphatidase Dzeta2 protein, and the somatotropic hormone sensibility and the low phosphorus stress responsibility of the plants to be related, genetically modified plants which have high sensibility to the somatotropic hormone or high responsibility to the low phosphorus stress can be prepared, thereby the application amount of field somatotropic hormone can be greatly decreased, the absorption of the plants to low phosphorus is increased, and the agricultural cost can be greatly reduced.

The invention provides a split cladosporium-expressed phosphatidase C strain, and is preserved in General Microbiological Center of China Committee of Culture Collection for Microorganisms with a preservation number of CGMCC No. 7508. The provided bacterial strain and phosphatidase C prepared by the preparation method are used for grease refining, degumming effect is good, and the bacterial strain and phosphatidase C prepared by the preparation method can be used for grease refining, additive and medicine fields.

The invention provides phosphatidase, a coding gene, a preparation method and application thereof. Specifically, the invention provides isolated polypeptide, which contains: (1) the 21st to 565th amino acid sequence of SEQ ID NO:2; or (2) a polypeptide derived from (1) after substitution, deletion or addition of one or more amino acids in the amino acid sequence of (1) and reservation of the 21st to 565th amino acid sequence of SEQ ID NO:2. The invention also relates to a polynucleotide sequence for coding the polypeptide, a nucleic acid construct containing the polynucleotide sequence, a host cell of the polynucleotide sequence or the nucleic acid construct, a composition containing the polypeptide, and application of the polypeptide.

The invention discloses a freeze-drying protective agent for sphingomyelinase and a preparation method, and belongs to the technical field of biology. The freeze-drying protective agent of sphingomyelinase comprises the following components: 0.2%-0.3% (w/v) of cane sugar, 0.1%-0.4% (w/v) of trehalose, 0.2%-0.5% (w/v) of D-mannitol, 0.1%-0.4% (w/v) of glycine, 0.02%-0.05% (v/v) of Tween 20 and 5-10 mM of Mg<2+>. Through formula screening and optimization, the obtained sphingomyelinase freeze-dried powder has the characteristics of high enzyme activity, good thermal stability, long storage time and good appearance.